• Journal of Conservation Science
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• v.38 no.3
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• pp.217-233
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• 2022

The remains found in Geumcheok-ri, Gyeongju are located in close proximity to the Ancient Tombs in Geumcheok-ri, Gyeongju (Historic Site) which were built between the late 5th and early 6th centuries, and these tombs are known to belong to the powerful rulers of that area. Using metallurgical techniques, this study was conducted on the iron objects obtained from the excavated remains in Geumcheok-ri, Gyeongju which are presumed to have a close relation to the nearby ruins that played an important role in the growth of Silla. To identify differences in manufacturing techniques based on the purpose of the iron objects, eight objects were selected after classifying them by use and the microstructure and non-metallic inclusions were investigated. The analyses results confirmed that the manufacturing process involved forging iron with a high or low carbon content to produce a particular shape, and that the carburization process was applied to iron post forging a shape to increase its strength when necessary. The mechanical properties were improved by selectively applying the steelmaking method and the heat treatment technique considering the functions of the parts, and the low temperature reduction was applied to the smelting process. Furthermore, in comparison with the iron objects excavated from the remains located in the center of Gyeongju and its outskirts, it is confirmed that there is similarity in the smelting and manufacturing techniques between these objects.

• The Journal of the Convergence on Culture Technology
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• v.8 no.5
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• pp.641-651
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• 2022

This research compares and analyzes evacuation time depending on the change in stair width in case of fire at Goshiwons. For this, a simulation has been conducted based on possible evacuation time according to the calculation method for the number of people admittable to a specific target for fire fighting equipped with accommodation. Currently, Gosiwon, which is classified as an accommodation facility (a total floor area of 500 m² or more), uses blind spots prescribed by the Fire Services Act, Building Act, and Parking Act to build a high-rise building on a small area of land, and most Gosiwon is transformed into a modified accommodation. This is in line with the owner's operating profit, so it is expected to show a continuous increase. Securing the golden time of Gosiwon evacuation time is the last bastion of Gosiwon residents who belong to the economically disadvantaged in our society, and we hope this study will serve as a starting point for discussions on revising related laws and regulations to establish a social safety net As a result of the evacuation simulation analysis, the evacuation time was the least when the width of the group and the evacuation stairs were expanded to 200cm, and the evacuation time of the existing building was reduced by up to 166.3 seconds by comparing 648.4 seconds and scenario 6. This analysis can be meaningful, in that the width of the evacuation stairs revision of related laws and regulations for the safety of multiplex available premises.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.3
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• pp.198-205
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• 1982

In 1980, rice was considerably damaged by abnormal low temperature. In this paper, to determine the effect of low temperature on the growth and nutrition of rice, the experiment were carried out: varietal response to low temperature in the region with different elevations. Regional differences of heading response to low temperature were observed among varieties. The difference of days between regions were bigger in tongil lines than Japonica lines. Especially, Milyang 42 and Hangangchalbeo might belong the cold suceptible group, since the varieties were severely delayed their heading in the high mountainous region as comparred to plain region. The delay of heading with low temperature was brought out grain sterility, and fertility and ripening ratio is influenced cold tolerance and elevations, and it's decreased yield. Varieties with higher grain sterility by low temperature have higher total nitrogen content, but tended to have lower potassium and phosphate contents in the flag leaf. High content of total nitrogen, low contents of potassium and silicate were observed in the sterilized grain chaff an the opposite result were noted in the ferilized grain chaff at the ripening stage. The results reveal that the balance of these mineral element may play an important role in ripening and possibly cold tolerance.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.73-81
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• 2023

Sirtuins (SIRTs) belong to the nicotinamide adenine dinucleotide (NAD+)-dependent class III histone deacetylase family. They are key regulators of cellular and physiological processes, such as cell survival, senescence, differentiation, DNA damage and stress response, cellular metabolism, and aging. SIRTs also influence carcinogenesis, making them potential targets for anticancer therapeutic strategies. In this study, we investigated the anticancer properties and underlying molecular mechanisms of a novel SIRT1 inhibitor, MHY2251, in human colorectal cancer (CRC) cells. MHY2251 reduced the viability of various human CRC cell lines, especially those with wild-type TP53. MHY2251 inhibited SIRT1 activity and SIRT1/2 protein expression, while promoting p53 acetylation, which is a target of SIRT1 in HCT116 cells. MHY2251 treatment triggered apoptosis in HCT116 cells. It increased the percentage of late apoptotic cells and the sub-G1 fraction (as detected by flow cytometric analysis) and induced DNA fragmentation. In addition, MHY2251 upregulated the expression of FasL and Fas, altered the ratio of Bax/Bcl-2, downregulated the levels of pro-caspase-8, -9, and -3 proteins, and induced subsequent poly(ADP-ribose) polymerase cleavage. The induction of apoptosis by MHY2251 was related to the activation of the caspase cascade, which was significantly attenuated by pre-treatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, MHY2251 stimulated the phosphorylation of c-Jun N-terminal kinase (JNK), and MHY2251-triggered apoptosis was blocked by pre-treatment with SP600125, a JNK inhibitor. This finding indicated the specific involvement of JNK in MHY2251-induced apoptosis. MHY2251 shows considerable potential as a therapeutic agent for targeting human CRC via the inhibition of SIRT1 and activation of JNK/p53 pathway.

• Journal of Life Science
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• v.33 no.1
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• pp.91-101
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• 2023

Bacteriocins are antimicrobial peptides synthesized on ribosomes, produced by bacteria, that inhibit the growth of similar or closely related bacterial strains. Since the discovery of nisin, many bacteriocins with unique structures and various modes of antibacterial activity have been described, and genes encoding production, secretion, and immunity have been reported. Nisin is one of the bacteriocins applied in cheese, liquid eggs, sauces and canned foods. Many of the bacteriocins of the genus Bacillus belong to lantibiotics, which are modified peptides after translation. Other genus Bacillus also produce many non-lantibiotic bacteriocins. Bacteriocins of the genus Bacillus are sometimes becoming more important because of their broader antibacterial spectrum. Bacteriocins are considered attractive compounds in the food and pharmaceutical industries to prevent food spoilage and growth of pathogenic bacteria. Bacteriocins can be used as biological preservatives in a variety of ways in the food system. Biopreservation refers to extending shelf life and improving safety of foods using microorganisms and/or their metabolites. The demand for new antimicrobial compounds has generated great interest in new technologies that can improve food microbiological safety. Applications of bacteriocins are expanding from food to human health. Today, many researchers are shifting their interest in bacteriocins from food preservation to the treatment of bacteria that cause infections and antibiotic-resistant diseases. This exciting new era in bacteriocin research will undoubtedly lead to new inventions and new applications. In this review, we summarize the various properties and applications of bacteriocins produced by the genus Bacillus.

• Parasites, Hosts and Diseases
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• v.61 no.3
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• pp.317-324
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• 2023

Standard- and large-sized eggs of Trichuris trichiura were found in the feces of schoolchildren in Yangon, Myanmar during epidemiological surveys and mass deworming with albendazole in 2017-2019. The standard-sized eggs were identified as those of T. trichiura, but it was necessary to exclude the possibility of the large-sized eggs belonging to Trichuris vulpis, a dog whipworm. We conducted morphological and molecular studies to determine the species of the 2 types of Trichuris eggs. Individual eggs of both sizes were isolated from Kato-Katz fecal smears (n=20) and mechanically destroyed using a 23G injection needle. Nuclear DNA was extracted, and the 18S rRNA region was sequenced in 15 standard-sized eggs and 15 large-sized eggs. The average size of standard-sized eggs (T. trichiura) was 55.2×26.1 ㎛ (range: 51.7-57.6×21.3-28.0 ㎛; n=97), whereas the size of large-sized eggs was 69.3×32.0 ㎛ (range: 65.1-76.4×30.1-34.5 ㎛; n=20), slightly smaller than the known size of T. vulpis. Regarding standard-sized eggs, the 18S rRNA nucleotide sequences exhibited 100% homology with T. trichiura deposited in GenBank and 88.6-90.5% homology with T. vulpis. Regarding large-sized eggs, the nucleotide sequences showed 99.8-100% homology with T. trichiura in GenBank and 89.6-90.7% homology with T. vulpis. Both standard- and large-sized eggs of Trichuris spp. found in Myanmar schoolchildren during 2017-2019 were morphologically and molecularly confirmed to belong to T. trichiura. The conversion of eggs from smaller to large sizes might be due to anthelmintic treatments with albendazole.

• Mycobiology
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• v.51 no.1
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• pp.36-48
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• 2023

Xanthoria elegans is a lichen symbiosis, that inhabits extreme environments and can absorb UV-B. We reported the de novo sequencing and assembly of X. elegans genome. The whole genome was approximately 44.63 Mb, with a GC content of 40.69%. Genome assembly generated 207 scaffolds with an N50 length of 563,100 bp, N90 length of 122,672 bp. The genome comprised 9,581 genes, some encoded enzymes involved in the secondary metabolism such as terpene, polyketides. To further understand the UV-B absorbing and adaptability to extreme environments mechanisms of X. elegans, we searched the secondary metabolites genes and gene-cluster from the genome using genome-mining and bioinformatics analysis. The results revealed that 7 NR-PKSs, 12 HR-PKSs and 2 hybrid PKS-PKSs from X. elegans were isolated, they belong to Type I PKS (T1PKS) according to the domain architecture; phylogenetic analysis and BGCs comparison linked the putative products to two NR-PKSs and three HR-PKSs, the putative products of two NR-PKSs were emodin xanthrone (most likely parietin) and mycophelonic acid, the putative products of three HR-PKSs were soppilines, (+)-asperlin and macrolactone brefeldin A, respectively. 5 PKSs from X. elegans build a correlation between the SMs carbon skeleton and PKS genes based on the domain architecture, phylogenetic and BGC comparison. Although the function of 16 PKSs remains unclear, the findings emphasize that the genes from X. elegans represent an unexploited source of novel polyketide and utilization of lichen gene resources.

• Informatization Policy
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• v.30 no.1
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• pp.41-66
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• 2023

This study aims to reveal the direction of municipal ordinances for smart cities, while focusing on 74 municipal ordinances from 72 municipal governments through topic modeling. As a result, the main keywords that show a high frequency belong to establishment and operations of the Smart City Committee. From the result of topic modeling Latent Dirichlet Allocation(LDA), it classifies municipal ordinances for smart cities into eight topics as follows: Topic 1(security for process of smart cities), Topic 2(promotion of smart city industry), Topic 3(composition of a smart city consultative body for local residents), Topic 4(support system for smart cities), Topic 5(management for personal information), Topic 6(use of smart city data), Topic 7(implementation for intelligent public administration), and Topic 8(smart city promotion). As for topic categorization by region, Topics 5, 6, and 8 which are mostly related to the practical operation of smart cities have a significant portion of municipal ordinances for smart cities in the Seoul metropolitan area. Then, Topics 2, 3, and 4 which are mostly related to the initial implementation of smart cities have a significant portion of municipal ordinances for smart cities in provincial areas.

• Korean Journal of Plant Taxonomy
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• v.53 no.1
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• pp.32-37
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• 2023

Spiraea prunifolia f. simpliciflora Nakai is a perennial shrub widely used for horticultural and medicinal purposes. We simultaneously obtained the complete plastid genome (plastome) and nuclear ribosomal gene transcription units, 45S nuclear ribosomal DNA (nrDNA) and 5S nrDNA of S. prunifolia f. simpliciflora, using Illumina short-read data. The plastome is 155,984 bp in length with a canonical quadripartite structure consisting of 84,417 bp of a large single-copy region, 18,887 bp of a short single-copy region, and 26,340 bp of two inverted repeat regions. Overall, a total of 113 genes (79 protein-coding genes, 30 tRNAs, and four rRNAs) were annotated in the plastome. The 45S nrDNA transcription unit is 5,848 bp in length: 1,809 bp, 161 bp, and 3,397 bp for 18S, 5.8S, and 26S, respectively, and 261 bp and 220 bp for internal transcribed spacer (ITS) 1 and ITS 2 regions, respectively. The 5S nrDNA unit is 512 bp, including 121 bp of 5S rRNA and 391 bp of intergenic spacer regions. Phylogenetic analyses showed that the genus Spiraea was monophyletic and sister to the clade of Sibiraea angustata, Petrophytum caespitosum and Kelseya uniflora. Within the genus Spiraea, the sections Calospira and Spiraea were monophyletic, but the sect. Glomerati was nested within the sect. Chamaedryon. In the sect. Glomerati, S. prunifolia f. simpliciflora formed a subclade with S. media, and the subclade was sister to S. thunbergii and S. mongolica. The close relationship between S. prunifolia f. simpliciflora and S. media was also supported by the nrDNA phylogeny, indicating that the plastome and nrDNA sequences assembled in this study belong to the genus Spiraea. The newly reported complete plastome and nrDNA transcription unit sequences of S. prunifolia f. simpliciflora provide useful information for further phylogenetic and evolutionary studies of the genus Spiraea, as well as the family Rosaceae.

• Korea Trade Review
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• v.42 no.4
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• pp.21-47
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• 2017

This study examines the effects of technological capability and domestic marketing capability of SMEs on the export market orientation, focusing on the moderating effect of market turbulence. This empirical analysis, based on surveys collected from 206 small and medium-sized firms located in Gwangju and Jeonnam provinces, yielded the following conclusions. First, technological capability of SMEs has a positive impact on export market orientation. This result not only is consistent with preceding studies but also suggests that when technological capability of SMEs is utilized as their own unique asset to reduce liability of smallness or liability of foreignness, the tendency to export might be increased. Second, domestic marketing capability of SMEs has a positive impact on export market orientation. This result suggests that as the domestic marketing capability of SMEs is used as prior knowledge reducing uncertainty to enter the overseas markets, the tendency to export might be increased. Third, as a result of examining moderating effect of market turbulence, it shows that market turbulence moderates the relationship between domestic marketing capability and export market orientation toward a negative(-) direction. That is, since market turbulence might weaken the relationship between domestic marketing capability and export market orientation, SMEs which belong to the industry considering marketing capability important need to develop strategy with consideration for market turbulence. This study provides theoretical and practical implications in that it reveals factors positively affecting export market orientation and market turbulence can be utilized as a moderator.