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Characterization of Ca2+-Dependent Protein-Protein Interactions within the Ca2+ Release Units of Cardiac Sarcoplasmic Reticulum

  • Rani, Shilpa;Park, Chang Sik;Sreenivasaiah, Pradeep Kumar;Kim, Do Han
    • Molecules and Cells
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    • v.39 no.2
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    • pp.149-155
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    • 2016
  • In the heart, excitation-contraction (E-C) coupling is mediated by $Ca^{2+}$ release from sarcoplasmic reticulum (SR) through the interactions of proteins forming the $Ca^{2+}$ release unit (CRU). Among them, calsequestrin (CSQ) and histidine-rich $Ca^{2+}$ binding protein (HRC) are known to bind the charged luminal region of triadin (TRN) and thus directly or indirectly regulate ryanodine receptor 2 (RyR2) activity. However, the mechanisms of CSQ and HRC mediated regulation of RyR2 activity through TRN have remained unclear. We first examined the minimal KEKE motif of TRN involved in the interactions with CSQ2, HRC and RyR2 using TRN deletion mutants and in vitro binding assays. The results showed that CSQ2, HRC and RyR2 share the same KEKE motif region on the distal part of TRN (aa 202-231). Second, in vitro binding assays were conducted to examine the $Ca^{2+}$ dependence of protein-protein interactions (PPI). The results showed that TRN-HRC interaction had a bell-shaped $Ca^{2+}$ dependence, which peaked at pCa4, whereas TRN-CSQ2 or TRN-RyR2 interaction did not show such $Ca^{2+}$ dependence pattern. Third, competitive binding was conducted to examine whether CSQ2, HRC, or RyR2 affects the TRN-HRC or TRN-CSQ2 binding at pCa4. Among them, only CSQ2 or RyR2 competitively inhibited TRN-HRC binding, suggesting that HRC can confer functional refractoriness to CRU, which could be beneficial for reloading of $Ca^{2+}$ into SR at intermediate $Ca^{2+}$ concentrations.

Analysis of Genes with Alternatively Spliced Transcripts in the Leaf, Root, Panicle and Seed of Rice Using a Long Oligomer Microarray and RNA-Seq

  • Chae, Songhwa;Kim, Joung Sug;Jun, Kyong Mi;Lee, Sang-Bok;Kim, Myung Soon;Nahm, Baek Hie;Kim, Yeon-Ki
    • Molecules and Cells
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    • v.40 no.10
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    • pp.714-730
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    • 2017
  • Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

Solar Photovoltaics Technology: No longer an Outlier

  • Kazmerski, Lawrence L.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.70-70
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    • 2011
  • The prospects of current and coming solar-photovoltaic (PV) technologies are envisioned, arguing this solar-electricity source is beyond a tipping point in the complex worldwide energy outlook. Truly, a revolution in both the technological advancements of solar PV and the deployment of this energy technology is underway; PV is no longer an outlier. The birth of modern photovoltaics (PV) traces only to the mid-1950s, with the Bell Telephone Laboratories' development of an efficient, single-crystal Si solar cell. Since then, Si has dominated the technology and the markets, from space through terrestrial applications. Recently, some significant shift toward technology diversity have taken place. Some focus of this presentation will be directed toward PV R&D and technology advances, with indications of the limitations and relative strengths of crystalline (Si and GaAs) and thin-film (a-Si:H, Si, Cu(In,Ga)(Se,S)2, CdTe). Recent advances, contributions, industry growth, and technological pathways for transformational now and near-term technologies (Si and primarily thin films) and status and forecasts for next-generation PV (nanotechnologies and non-conventional and "new-physics" approaches) are evaluated. The need for R&D accelerating the now and imminent (evolutionary) technologies balanced with work in mid-term (disruptive) approaches is highlighted. Moreover, technology progress and ownership for next generation solar PV mandates a balanced investment in research on longer-term (the revolution needs revolutionary approaches to sustain itself) technologies (quantum dots, multi-multijunctions, intermediate-band concepts, nanotubes, bio-inspired, thermophotonics, ${\ldots}$ and solar hydrogen) having high-risk, but extremely high performance and cost returns for our next generations of energy consumers. This presentation provides insights to the reasons for PV technology emergence, how these technologies have to be developed (an appreciation of the history of solar PV)-and where we can expect to be by this mid-21st century.

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Compressibility of $FeS_{2}$ ($FeS_{2}$의 압축성 연구)

  • Kim, Young-Ho;Hwang, Gil-Chan;Cho, Hyen-Goo
    • Journal of the Mineralogical Society of Korea
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    • v.19 no.3 s.49
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    • pp.189-195
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    • 2006
  • Compression work on a pyrite powder has been carried out using energy dispersive X-ray diffraction (EDXRD) with Mao-Bell type diamond anvil cell (DAC) and synchrotron radiation(SR) at room temperature. It has been reported the bulk moduli of pyrite show the large variations depending on the experimental conditions as well as the apparatus used. Thus, two kinds of sample in different pressure transmitting media of both NaCl and MgO powder emerged in alcoholic fluids were subjected to measure their compressibilities. Bulk moduli thus obtained are 138.9 GPa and 198.2 GPa, respectively, and this result contradicts to the anticipated values according to the hydrostaticity conditions of the sample chamber. This might be due to the alcoholic fluids phase transition mainly with the side effects from the difference of both solid state detector (SSD) used and E*d value applied. All experiments were performed at the Beam Line 1B2 of Pohang Light Source (PLS).

A Study on Fashion Souvenir Ornament Design for Female Dolls with the Application of Relic and Painting Motifs in the Late Goryeo Period -Focus on Investigated Design and Costume Coordination- (고려 말기 복식유물문양과 회화자료를 응용한 여성형 인형장신구 문화상품 디자인 연구 -고증디자인 및 복식 코디네이션을 중심으로-)

  • Choi, Jeong
    • Journal of the Korean Society of Clothing and Textiles
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    • v.37 no.5
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    • pp.691-703
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    • 2013
  • The costume culture of ladies in late Goryeo is an important and rare source for cultural fashion souvenir design. This study investigates the historical costume design sources of late Goryeo to suggest high quality traditional and practical ornament souvenir designs for female dolls as well as examples of coordination with doll costumes. The costume culture of late Goryeo contained golden Buddhism and a revival of $12^{th}$ century Goryeo costume characteristic after the period of Mongolian influences from the Yuan. The main sources for doll ornament designs are $14^{th}$ century Buddhist paintings, a few ornament relics of Goryeo, ancient literature, and portraits of ladies in the $14^{th}$ century that originated from five doll's traditional ornaments (lotus hairpin, cloud-shaped hairpin, Buddhist flower hair ornament, vase-shaped Binyeo, and bell of animalhuman face) and three wig designs (wigs from Buddhist paintings and portraits, and hairpiece) with bleached gilding silver, silk, various gemstones and synthetic hair. These doll ornament and wig designs imply the symbol of Buddhism, exorcism, and costume culture of $14^{th}$ century Goryeo ladies that can be matched with two doll gowns, half-sleeved jacket, skirt, two Jogoris, apron, under trousers as formal, semi-formal, and town-wear according to the T.P.O of late Goryeo; in addition, five ornaments can be used as owner's ornaments. The partial changing colors and materials due to the characteristics of doll ornament goods as well as better communication between researcher and constructor remain to be improved.

The performance of a wedge type jellyfish excluder device inserted in a trawl net (트롤 어구에 부착된 쐐기형 해파리 분리배출장치의 성능 연구)

  • Park, Chang-Doo;Kim, In-Ok;Lee, Kyoung-Hoon;Lee, Gun-Ho;Park, Seong-Wook
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.46 no.4
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    • pp.302-312
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    • 2010
  • In order to describe the performance of a wedge type jellyfish excluder device, a series of fishing experiments was carried out in the coastal areas of Yokji Island, southern Korea in 2009, using a trawl net with a cover net. The body size and weight of each individual (fish or jellyfish) caught in the experimental fishing were measured. In the case of giant jellyfish the bell diameter and weight were measured. The catch species was composed of giant jellyfish (Nemopilema nomurai), silver croaker (Pennahia argentata), yellow croaker (Larimichthys polyactics), finespotted flounder (Pleuronichthys cornutus), largehead hairtail (Trichiuruslepturus), melon seed (Psenopsisanomala) and so on. The weight ratio and individual ratio of total fish escaped through the outlet of the excluder device were 0.322 and 0.320, respectively. The weight ratios of giant jellyfish excluded from the trawl net ranged from 0.740 to 0.921 (average 0.852/haul). It means that the wedge type jellyfish excluder device performed well and allowed the most of the giant jellyfish to exclude through the trawl net. The approximately 70% of fish entered in trawl net was caught. The wedge type excluder device needs some improvements to minimize the fish escape from the trawl nets in the future.

A Farm on Broiler Chickens' Welfare in Korea (동물복지 관련 육계농가 실태조사)

  • Cheon, Si-Nae;Lee, Jun-Yeob;Choi, Yang-Ho;Park, Kyu-Hyun;Kim, Doo-Hwan;Song, Jun-Ik;Jeon, Jung-Hwan
    • Journal of Animal Environmental Science
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    • v.19 no.1
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    • pp.33-38
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    • 2013
  • We conducted investigations of the information and welfare of broiler chickens in Korea. The livestock housing of all broiler chicken farms were windowless type, bell feeder and nipple waterer were used, and stocking density was relatively higher (ranging from 18.2 $birds/m^2$ to 24.2 $birds/m^2$) than RSPCA's welfare standards for chicken hens (less than 19 $birds/m^2$). The ratios of sandwich panel, urethane form, slate, and steel plate in roofing materials were 61%, 21%, 13%, and 5%, respectively. The ratios of sandwich panel, urethane form, brick, and steel plate in wall materials were 61%, 21%, 13%, and 5%, respectively. The ratios of soil and concrete in flooring materials were 10% and 90%. The mist spray, fan, and cooling pad in cooling facilities were 42%, 32%, and 26%, respectively. Thus we believe that present data contribute to develop the animal welfare certification for broiler chickens and to improve animal welfare in Korea.

A Study on the Hoop Applied on Contemporary Fashion (현대(現代)패션에 응용(應用)된 후프(Hoop)에 관(關)한 연구(硏究))

  • Jeong, Kyung-Hee;Bae, Soo-Jeong
    • Journal of Fashion Business
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    • v.8 no.2
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    • pp.126-140
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    • 2004
  • The purpose of this study is to examine the fashion design applying to the hoop, which have become various since 1980. To do this, this study is investigated the origin and the changes of the hoop historically, then classified its types, and finally analyze the collections from 1980 to 2003. The hoop was appeared on the late 15th century. Then in the mid 16th century, bell-shaped farthingale was prevailed, followed by drum-shaped wheel farthingale and hausse-cul in England and France. In the 17th century, slim silhouette was popular, so that the hoop declined gradually. In the 18th century, panier and panier double were introduced which is to make side-expanded skirt silhouette. In the 19th century, crinoline and bustle were created. The hoops applied to modern collections are used various materials, techniques, and forms. It is a main item of historicism which appeared in the trend of post-modernism and the phenomenon of applying underwear to an outer garment like corset. The hoop is adapted into three ways: only with the frame of hoop, wearing a skirt with some parts of the frame exposed on purpose, and wearing a skirt on the frame, thus not showing the frame. Thus, it is thought that wearing a hoop as a frame is used an element of recreation of past fashion, at the same time it was affected by historicism which is a branch of post-modernism. Applying the hoop as an outer garment may be affected by eroticism which changed underwear into an outer wear in postmodernism and deconstructivism.

A Study on the Spatial Composition Characteristics of Elderly Housing facilities in Korea (한국 노인주거시설의 공간계획 특성에 관한 연구)

  • Oh Hye Kyung;Hong Yi Kyung;Him Hyun Jee
    • Journal of Families and Better Life
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    • v.22 no.6 s.72
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    • pp.133-142
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    • 2004
  • The purpose of this study was to investigate the spatial composition characteristics of elderly housing facilities in Korea, in order to refer them in establishing the basic plan for elderly housing in Korea. The case study performed in this study from October 16 to October 31 is based on ten housing facilities for the elderly in KOREA. During the site visits, the interview with the custodians, survey of the space, picture taking and diagram gathering were carried out. The results of this study were as follows; 1. The type of floor plan was classified into ㅁ type, L type, and ㅡ type. And they hadn't an outside space called a garden(courtyard). 2. The kinds of common spaces could be grouped as the space for basic living, for leisure, and for convenient living. Also it was all common that the housing facility had common diningroom laundry room once, and activity room. The type of the common spaces was categorized into three types, the centralized type where the shared space was located at the center of the housing facilities, the distributed type, depending on the degree of separation between common spaces and units, and another each building type. 3. The kinds of units was classified into studio, oneroom, one bedroom and two bedroom. 4. Elevator, safety grab bar, emergency bell, and the door handle of lever or bar type were placed in these facilities.

Performance of Hybrid DS/FH Spread-Spectrum Systems in Cellular Packet Radio Network (셀룰러 패킷무선망에서 하이브리드 DS/FH 확산대역 시스팀의 성능)

  • 조현욱;박상규
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.10A
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    • pp.1462-1470
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    • 1999
  • In this paper, the performance of packet radio networks using the ALOHA protocol in Rayleigh fading channels is analyzed in terms of the capture probability and the throughput. We consider capture effect in order to increase the probability of the channel access and to include cellular environment we assume that the traffic is a truncated bell-shaped distribution. The packet proposed in this paper consists of a competition part and a data part to decrease the probability of collision in the channel access. In the competition part, DS system with common spreading code is used and in the data, hybrid DS/FH system is used. We consider the linear correlation receiver and the hard-limiting correlation receiver as the receiver model. BPSK modulation is also employed. Because hybrid DS/FH systems are not sensitive to variation of the interference power, as we choose a proper chip number N and a frequency hopping number q, we can obtain a steady system performance even though the variation of interference power is severe fading channels such as Rayleigh fading.

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