• Title/Summary/Keyword: batch experiment

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Analysis on the distribution of nitrogen and phosphorus removing microorganisms and nitrifying activity in a trickling filter (살수여상에서의 질소, 인 제거 미생물 분포 및 질산화 활성 조사)

  • Kim, Dong-Jin;Yoo, Ik-Keun;Ahn, Dae-Hee
    • Journal of Environmental Science International
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    • v.18 no.6
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    • pp.691-698
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    • 2009
  • Trickling filter has been extensively studied for the domestic wastewater treatment especially for the small scale plants in rural area. The performance of the trickling filter depends on the microbial community and their activity in the biofilms on the media. Nitrification. denitrification, and phosphorus removal of the trickling filter from the wastewater depend on the activity and the amount of the specific microorganisms responsible for the metabolism. For the estimation of the performance of a trickling filter, batch nitrification experiment and fluorescence in situ hybridization (FISH) were carried out to measure the microbial activity and its distribution on the media of the trickling filter. Batch nitrification activity measurement showed that the top part of the 1st stage trickling filter had the highest nitrification activity and the maximum activity was 0.002 g $NH_4$-N/g MLVSS${\cdot}$h. It is thought that higher substrate (ammonia) concentration yields more nitrifying bacteria in the biofilms. The dominant ammonia oxidizer and nitrite oxidizer in the biofilm were Nitrosomonas species and genus Nitrospira, respectively, by FISH analysis. Less denitrifiers were found than nitrifiers in the biofilm by the probe Rrp1088 which specifically binds to Rhodobacter, Rhodovulum, Roseobacter, and Paracoccus. Phosphorus accumulating bacteria were mostly found at the surface of the biofilm by probe Rc988 and PAO651 which specifically binds to Rhodocyclus group and their biomass was less than that of nitrifiers.

Bio-kinetic and Design Analysis of a Sequencing Batch Reactor by Aerobic Granular Sludge (호기성 입상화 슬러지를 이용한 SBR 운전의 동력학적 해석 및 설계분석)

  • Choi, Seong-Woo
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.4
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    • pp.275-280
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    • 2011
  • In the present work, the aerobic particle's characteristics were enhanced. A polymer was used to make aerobic granular sludge in short period of time. And operation parameters were calculated for organic matter removal in domestic wastewater using a sequencing batch reactor (SBR). The experiment for sewage (Influent concentration of 63~72 mg COD/L) by using mature aerobic granular sludge showed the organic matter removal rate k and oxygen utilization coefficient a', b' were $10.161d^{-1}$ and 0.87 mg $O_2/mg$ $COD_r$, 0.11 mg $O_2/mg$ MLVSS d respectively. Therefore, it was more effective than K value $5{\sim}8d^{-1}$ of conventional activated sludge process. The sludge synthetic value and sludge auto-oxydation value were 0.45 mg VSS/mg $COD_r$ and 0.05 mg VSS/mg MLVSS d respectively. Consequently, mortality rates of microorganisms was lower than conventional activated sludge process.

Hydrogen Production and Organic Removal according to Mixture Ratio of Food Wastewater and Swine Wastewater using Anaerobic Batch Reactor (회분식 혐기성 소화 반응기에서 음식물탈리액과 양돈폐수의 혼합비에 따른 수소 생산 및 유기물 제거)

  • Kim, Choong-Gon;Kang, Seon-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.5
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    • pp.641-647
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    • 2007
  • This study aims to find out optimum condition for hydrogen production and organic removal when treating food and swine wastewater together. For this purpose, various batch tests were conducted by changing mixture ratio from 6:4 (food wastewater:swine wastewater) to 1:9 without pretreatment process. For hydrogen production through anaerobic fermentation, the mixture ratios of R-1 (6:4), R-2 (5:5) and R-6 (1:9) were out of pH range appropriate for hydrogen production and mixture ratios of R-3 (4:6), R-4 (3:7), and R-5(2:8) showed appropriate hydrogen production where their pH ranges were 5.1~5.5. Especially in case of R-3, it consistently maintained appropriate pH range for hydrogen production for 72hr and produced maximum hydrogen. The characteristics of hydrogen production and cumulative hydrogen production according to each mixture ratio showed that R-1, R-2 and R-6 did not produce any hydrogen, and maximum hydrogen productions of R-3, R-4 and R-5 were 593ml, 419ml and 90ml, respectively. Total cumulative hydrogen productions of R-3, R-4 and R-5 were 1690ml, 425ml and 96ml, respectively. Based on previous results, it was concluded that, the most appropriate mixture ratio of food wastewater and swine wastewate rwas 4:6 (R-3). The experiment for COD removal rate to evaluate organic removal efficiency revealed that R-3, R-4 and R-5 showed high removal efficiencies during the highest hydrogen production amount and the highest efficiency was 41% with R-3.

Optimization of Substract Concentration in Cell Production of Fungal Chitosan (균류키토산의 균체생산에서 기질농도 최적화에 관한 연구)

  • 김봉섭;서명교;노종수;이용희;이국의
    • Journal of Environmental Health Sciences
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    • v.29 no.3
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    • pp.72-78
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    • 2003
  • In the process of producing chitosan from crustacean shell, the use of excessive acid and alkli is causing the problems of environmental pollution and of production cost. In this study, one way to solve these problems is to cultivate fungi, then, to extract chitosan from the cell wall. By means of flask incubation and batch cultivation, the optimum cultivation conditions for mass production of continuous cultivation was found. Four strains used for the production of fungal chitosan were Gongronella butleri IF08080, Absidia coerulea IF05301, Rhizopus delemar IF04775, Mucor tuberculisporus IF09256. In flask incubation to select strain of producing much chitosan by means of experiment of the effect of initial pH, Absidia coerulea IFO 5301 had highest yield in FCs, 258.1 $\pm$ 47.3 mg/200 $m\ell$l at pH 6.5. In flask incubation under the optimum cultivation condition, temperature 27$^{\circ}C$, culture time 6days, glucose 2%, peptone 1%, (NH$_4$)$_2$ SO$_4$ 0.5%, $K_2$HPO$_4$ 0.1 %, Nacl 0.1 %, MgSO$_4$ㆍ7$H_2O$ 0.05%, CaCl$_2$ㆍ2$H_2O$ 0.01 %, the yield of DCW brought the highest yields. In batch bioreactor, the optimum cultivation condition was that cell suspended solution was 70 $m\ell$, aeration rate 0.5 l/min, agitation rate 800 rpm, culture time 36 hr. In continuous bioreactor, the optimum substrate flow rate was 4 ι/day.

Bacteriophage removal in various clay minerals and clay-amended soils

  • Park, Jeong-Ann;Kang, Jin-Kyu;Kim, Jae-Hyun;Kim, Song-Bae;Yu, Seungho;Kim, Tae-Hun
    • Environmental Engineering Research
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    • v.20 no.2
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    • pp.133-140
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    • 2015
  • The aim of this study was to investigate the bacteriophage removal in various clay minerals and clay-amended soils. Batch experiments in kaolinite, montmorillonite, and bentonite showed that kaolinite was far more effective at the MS2 removal than montmorillonite and bentonite. In kaolinite, the log removal increased from 0.046 to 2.18, with an increase in the adsorbent dose from 0.3 to $50g\;L^{-1}$, whereas the log removals in montmorillonite and bentonite increased from 0.007 to 0.40 and from 0.012 to 0.59, respectively. The MS2 removal in kaolinite-amended silt loam soils was examined at three different soil-to-solution (STS) ratios. Results indicated that the log removal of MS2 increased with an increase in the kaolinite content and the STS ratio. At the STS ratio of 1:10, the log removal of MS2 increased from 2.33 to 2.80 with an increase in the kaolinite content from 0% to 10% in kaolinite-amended soils. The log removals of MS2 at the STS ratios of 1:2 and 1:1 increased from 2.84 to 3.47 and from 3.46 to 4.76, respectively, with an increase in the kaolinite content from 0% to 10%. Results also indicated that the log removals of PhiX174 and $Q{\beta}$ in kaolinite-amended soils were similar to each other, but they were far lower than those of MS2 at all the kaolinite contents. The log removal of PhiX174 increased from 0.16 to 0.32, whereas the log removal of $Q{\beta}$ changed from 0.17 to 0.22 with an increase in the kaolinite content from 0% to 10%.

Characteristics of Aerobic Granular Activated Sludge According to Electron Acceptors in Sequencing Batch Reactor Process (SBR공정에서 전자수용체에 따른 호기성 입상활성슬러지의 공정별 특성)

  • Kim, I-Tae;Lee, Hee-Ja;Bae, Woo-Keun
    • Journal of Korean Society on Water Environment
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    • v.20 no.5
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    • pp.480-487
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    • 2004
  • This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

Characterization of Quintinite Particles in Fluoride Removal from Aqueous Solutions

  • Kim, Jae-Hyun;Park, Jeong-Ann;Kang, Jin-Kyu;Son, Jeong-Woo;Yi, In-Geol;Kim, Song-Bae
    • Environmental Engineering Research
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    • v.19 no.3
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    • pp.247-253
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    • 2014
  • The aim of this study was to characterize quintinite in fluoride removal from aqueous solutions, using batch experiments. Experimental results showed that the maximum adsorption capacity of fluoride to quintinite was 7.71 mg/g. The adsorption of fluoride to quintinite was not changed at pH 5-9, but decreased considerably in highly acidic (pH < 3) and alkaline (pH > 11) solution conditions. Kinetic model analysis showed that among the three models (pseudo-first-order, pseudo-second-order, and Elovich), the pseudo-second-order model was the most suitable for describing the kinetic data. From the nonlinear regression analysis, the pseudo-second-order parameter values were determined to be $q_e=0.18mg/g$ and $k_2=28.80g/mg/hr$. Equilibrium isotherm model analysis demonstrated that among the three models (Langmuir, Freundlich, and Redlich-Peterson), both the Freundlich and Redlich-Peterson models were suitable for describing the equilibrium data. The model analysis superimposed the Redlich-Peterson model fit on the Freundlich fit. The Freundlich model parameter values were determined from the nonlinear regression to be $K_F=0.20L/g$ and 1/n=0.51. This study demonstrated that quintinite could be used as an adsorbent for the removal of fluoride from aqueous solutions.

Improved Feature Extraction Method for the Contents Polluter Detection in Social Networking Service (SNS에서 콘텐츠 오염자 탐지를 위한 개선된 특징 추출 방법)

  • Han, Jin Seop;Park, Byung Joon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.11
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    • pp.47-54
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    • 2015
  • The number of users of SNS such as Twitter and Facebook increases due to the development of internet and the spread of supply of mobile devices such as smart phone. Moreover, there are also an increasing number of content pollution problems that pollute SNS by posting a product advertisement, defamatory comment and adult contents, and so on. This paper proposes an improved method of extracting the feature of content polluter for detecting a content polluter in SNS. In particular, this paper presents a method of extracting the feature of content polluter on the basis of incremental approach that considers only increment in data, not batch processing system of entire data in order to efficiently extract the feature value of new user data at the stage of predicting and classifying a content polluter. And it comparatively assesses whether the proposed method maintains classification accuracy and improves time efficiency in comparison with batch processing method through experiment.

Verification Algorithm for the Duplicate Verification Data with Multiple Verifiers and Multiple Verification Challenges

  • Xu, Guangwei;Lai, Miaolin;Feng, Xiangyang;Huang, Qiubo;Luo, Xin;Li, Li;Li, Shan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.15 no.2
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    • pp.558-579
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    • 2021
  • The cloud storage provides flexible data storage services for data owners to remotely outsource their data, and reduces data storage operations and management costs for data owners. These outsourced data bring data security concerns to the data owner due to malicious deletion or corruption by the cloud service provider. Data integrity verification is an important way to check outsourced data integrity. However, the existing data verification schemes only consider the case that a verifier launches multiple data verification challenges, and neglect the verification overhead of multiple data verification challenges launched by multiple verifiers at a similar time. In this case, the duplicate data in multiple challenges are verified repeatedly so that verification resources are consumed in vain. We propose a duplicate data verification algorithm based on multiple verifiers and multiple challenges to reduce the verification overhead. The algorithm dynamically schedules the multiple verifiers' challenges based on verification time and the frequent itemsets of duplicate verification data in challenge sets by applying FP-Growth algorithm, and computes the batch proofs of frequent itemsets. Then the challenges are split into two parts, i.e., duplicate data and unique data according to the results of data extraction. Finally, the proofs of duplicate data and unique data are computed and combined to generate a complete proof of every original challenge. Theoretical analysis and experiment evaluation show that the algorithm reduces the verification cost and ensures the correctness of the data integrity verification by flexible batch data verification.

Experimental Assessment of Mesophilic and Thermophilic Batch Fermentative Biohydrogen Production from Palm Oil Mill Effluent Using Response Surface Methodology

  • Azam Akhbari;Shaliza Ibrahim;Low Chin Wen;Afifi Zainal;Noraziah Muda;Liyana Yahya;Onn Chiu Chuen;Farahin Mohd Jais;Mohamad Suffian bin Mohamad Annuar
    • Korean Chemical Engineering Research
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    • v.61 no.2
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    • pp.278-286
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    • 2023
  • The present work evaluated the production of biohydrogen under mesophilic and thermophilic conditions through dark fermentation of palm oil mill effluent (POME) in batch mode using the design of experiment methodology. Response surface methodology (RSM) was applied to investigate the influence of the two significant parameters, POME concentration as substrate (5, 12.5, and 20 g/l), and volumetric substrate to inoculum ratio (1:1, 1:1.5, and 1:2, v/v.%), with inoculum concentration of 14.3 g VSS/l. All the experiments were analyzed at 37 ℃ and 55 ℃ at an incubation time of 24 h. The highest chemical oxygen demand (COD) removal, hydrogen content (H2%), and hydrogen yield (HY) at a substrate concentration of 12.5 g COD/l and S:I ratio of 1:1.5 in mesophilic and thermophilic conditions were obtained (27.3, 24.2%), (57.92, 66.24%), and (6.43, 12.27 ml H2/g CODrem), respectively. The results show that thermophilic temperature in terms of COD removal was more effective for higher COD concentrations than for lower concentrations. Optimum parameters projected by RSM with S:I ratio of 1:1.6 and POME concentration of 14.3 g COD/l showed higher results in both temperatures. It is recognized how RSM and optimization processes can predict and affect the process performance under different operational conditions.