Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Experimental Assessment of Mesophilic and Thermophilic Batch Fermentative Biohydrogen Production from Palm Oil Mill Effluent Using Response Surface Methodology

  • Azam Akhbari (Higher Institution Centre of Excellence (HICoE), UM Power Energy Dedicated Advanced Centre (UMPEDAC), Level 4, Wisma R&D, University of Malaya) ;
  • Shaliza Ibrahim (Institute of Ocean and Earth Sciences (IOES), University of Malaya) ;
  • Low Chin Wen (Institute of Ocean and Earth Sciences (IOES), University of Malaya) ;
  • Afifi Zainal (TNB Research Sdn.Bhd., No 1, Lorong Ayer Itam, Kawasan Institusi Penyelidikan) ;
  • Noraziah Muda (TNB Research Sdn.Bhd., No 1, Lorong Ayer Itam, Kawasan Institusi Penyelidikan) ;
  • Liyana Yahya (TNB Research Sdn.Bhd., No 1, Lorong Ayer Itam, Kawasan Institusi Penyelidikan) ;
  • Onn Chiu Chuen (Department of Civil Engineering, Faculty of Engineering, University of Malaya) ;
  • Farahin Mohd Jais (Department of Civil Engineering, Faculty of Engineering, University of Malaya) ;
  • Mohamad Suffian bin Mohamad Annuar (Institute of Biological Sciences, University of Malaya)
  • Received : 2022.08.29
  • Accepted : 2022.11.15
  • Published : 2023.05.01
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Abstract

The present work evaluated the production of biohydrogen under mesophilic and thermophilic conditions through dark fermentation of palm oil mill effluent (POME) in batch mode using the design of experiment methodology. Response surface methodology (RSM) was applied to investigate the influence of the two significant parameters, POME concentration as substrate (5, 12.5, and 20 g/l), and volumetric substrate to inoculum ratio (1:1, 1:1.5, and 1:2, v/v.%), with inoculum concentration of 14.3 g VSS/l. All the experiments were analyzed at 37 ℃ and 55 ℃ at an incubation time of 24 h. The highest chemical oxygen demand (COD) removal, hydrogen content (H2%), and hydrogen yield (HY) at a substrate concentration of 12.5 g COD/l and S:I ratio of 1:1.5 in mesophilic and thermophilic conditions were obtained (27.3, 24.2%), (57.92, 66.24%), and (6.43, 12.27 ml H2/g CODrem), respectively. The results show that thermophilic temperature in terms of COD removal was more effective for higher COD concentrations than for lower concentrations. Optimum parameters projected by RSM with S:I ratio of 1:1.6 and POME concentration of 14.3 g COD/l showed higher results in both temperatures. It is recognized how RSM and optimization processes can predict and affect the process performance under different operational conditions.

Keywords

Acknowledgement

The authors acknowledge the profound financial support from TNB Research Sdn. Bhd., Malaysia through Fundamental Research Grant Scheme PV035-2021.

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