• Journal of Food Hygiene and Safety
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• v.35 no.1
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• pp.45-50
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• 2020

In this study, seafood products (n=26) sold on sushi buffet restaurants in the city of Wonju were monitored by analyzing sequences of DNA barcode markers (cytochrome c oxidase subunit I and 16S ribosomal RNA genes). NCBI BLAST database was screened with the barcode sequences analyzed as a query for species identification. The BLAST search revealed that fifteen samples (58%) analyzed were consistent with their labeling information; however, the ingredients used in seven samples (27%) were not compliant with their label information. In the case of these mislabeled products, ingredients for sutchi catfish sushi and cherry bass sashimi were identified as Pangasianodon hypophthalmus and Lampris guttatus, respectively. For Japanese flying-fish roe sushi and Pacific herring roe sushi, roe of Mallotus villosus was used as an ingredient. Amphioctopus fangsiao and A. membranaceus were used in octopus sushi and soybean-marinated squid products, respectively. This monitoring result can contribute to the protection of consumer rights and the reduction of fraudulent practices in the food industry.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.10
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• pp.2242-2250
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• 2009

Recently, with the advance of Ubiquitous era, the types of services become diverse. Especially, due to the rapid development of mobile technology, the new functions of mobile phones are added and the new applications of mobile phones are developed actively. Among the various applications related to mobile phones, 2 dimensional barcode-based applications are increasing. 2 dimensional barcode is mostly used for the management of past record. However, by combing 2 dimensional barcode with mobile phones, the application areas of 2 dimensional barcode are expanded to the means of publicity for education, tourism, and festivals. In this paper, we develop a QR code decoder running on smartphones, which connects on-line and off-line. In addition, we modify our decoder by detecting the point for performance enhancement based on TRIZ. We compare our decoder with an open-source based decoder in terms of the code size of decoding and the speed of decoding in order to prove that our decoder has a better performance than the other. Finally, we introduce two applications: u-map and u-pamphlet as QR code applications.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.3
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• pp.276-281
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• 2019

Food printers must use food ink cartridges approved by the Ministry of Food and Drug Safety (MFDS). A 2D bar code is used to read whether the ink cartridge is authentic. However, since the dye is diverged by heat pressure and printed, the barcode is contaminated. In this paper, we propose a pre-processing algorithm to solve the problem of barcode contamination by food coloring dust in a latte art printer. The algorithm is based on various morphological operations. We apply this algorithm before reading contaminated barcode images with a general QR code reader. It has been confirmed that, as compared with the existing QR code reader, the contamination rate that can be perceived is increased from 25% to 40% and even at a contamination rate of 45%, the recognition rate reaches 50%.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.75-84
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• 2013

Objectives : The origin of Breeae Herba (So-gye) and Cirsii Herba (Dae-gye) is differently prescribed in Korean and Chinese modern pharmacopoeia. Since the similar morphological characteristics and chaotic plant names, moreover, the aerial part of Carduus crispus have been used as the Cirsii Herba. To develop a reliable method for correct identification of these herbal medicines and to evaluate the genetic relationship of these closely related plant species, we analyzed sequences of DNA barcode regions. Methods : Thirty-one samples of 6 medicinal plants (B. segeta, B. setosa, C. japonicum var. maackii, C. setidens, C. chanroenicum, and C. crispus) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL) were analyzed after amplification using appropriate primers reported in previous studies. The nucleotides of species-specific authentic marker and phylogenetic relations were estimated based on the entire sequences of DNA barcodes by the analysis of ClastalW and UPGMA, respectively. Results : In comparative analysis of DNA barcode sequences, we obtained specific nucleotides to discriminate the medicinal plant of Breeae/Cirsii Herba in species level and evaluated the phylogenetic relationship of these species. Futhermore, we identified distinct marker nucleotides enough to authenticate respective species. These sequence differences at corresponding positions were avaliable genetic markers to determine the botanical origins of Breeae Herbal as well as Cirsii Herba. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by providing of definitive information that can identify each plant species and distinguish from unauthentic adulterants and substitutes.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.53-58
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• 2013

Objectives : Pinelliae Tuber has been used as a typical unauthentic herbal medicines. Due to the morphological similarity between Pinelliae Tuber and adulterants, the correct authentication is very difficult. Therefore, we introduced DNA barcode to establish a powerful tool for the authentication of Pinelliae Tuner from adulterants. Methods : To obtain DNA barcode regions, genomic DNA was extracted from nineteen specimens of Pinellia ternata, Pinellia pedatisecta, Pinellia tripartita, and Typhonium flagelliforme, and matK and rbcL genes were amplified. For identification of species specific sequences and analysis phylogenetic relationship, a comparative analysis were performed by the ClastalW and UPGMA based on entire sequences of matK and rbcL genes, respectively. Results : In comparison of two DNA barcode sequences, we elucidated the phylogenetic relationship showing distinct four groups depending on species and identified 40 and 20 species specific nucleotides enough to distinguish each species from matK and rbcL gene, respectively. The sequence differences at the corresponding positions were avaliable genetic marker nulceotides to discriminate the correct species among analyzed four species. These results indicated that phylogentic and comparative analysis of matK and rbcL genes are useful genetic markers to authenticate Pinelliae Tubers. Conclusions : The marker nucleotides enough to distinguish P. ternata, P. tripatrita, P. peditisecta, and T. flagelliform, were observed at 40 positions in matK gene and 20 positions in rbcL gene sequence, respectively. These differences can be used to authenticate Pinelliae Tuber from adulterants as well as discriminate each four species.

• Journal of Ecology and Environment
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• v.34 no.3
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• pp.323-331
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• 2011

Biodiversity and the community composition of micro-eukaryotic organisms were investigated in the Upo wetland in Korea using molecular analysis. Molecular identification was performed using cytochrome oxidase I (COI) and small subunit ribosomal DNA (SSU rDNA). The genomic DNA was isolated directly from soil samples. The COI and SSU rDNA regions were amplified using universal primers and then sequenced after cloning. In a similarity search of the obtained sequences with BLAST in the Genbank database, the closely related sequences from NCBI were used to identify the amplified sequences. A total of six eukaryotic groups (Annelida, Arthropoda, Rotifera, Chlorophyta, Bacillariophyta, and Stramenopiles) with COI and six groups (Annelida, Arthropoda, Rotifera, Alveolata, Fungi, and Apicomplexa) with SSU rDNA genes were determined in the Upo wetland. Among 38 taxa in 20 genera, which are closely related to the amplified sequences, 10 genera (50%) were newly reported in Korea and five genera (25%) were shown to be distributed in the Upo wetland. This approach is applicable to the development of an efficient method for monitoring biodiversity without traditional taxonomic processes and is expected to produce more accurate results in depositing molecular barcode data in the near future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.77-78
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• 2015

Image Binarization is essential process in the digital image processing for the read out of a one-dimensional barcode. Local threshold method is suitable for binarization of a bar code. However, It has problem that processing time is slower than other binarization algorithm. Also, It's results not appropriate If the image has a noise. In this paper, we propose the modification method for solve these problems. Proposed algorithm help to improve the speed of local thresholding method using average image. Also, we proposed a high frequency filter to one-dimensional barcode for improvement quality of binary image.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.947-948
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• 2015

IOT(Internet of things) is a technology to embed various sensors and communication features on diverse things such as home appliances, mobile devices, wearable computers and connect to the Internet and through this technology, the status of things connected to the network can be analyzed and controlled with various data. On the other hand, this paper suggests to develop a merchandise management app using Android-based barcode to systematically manage expiry date of various goods that we purchase in our daily life. Therefore, individual goods are recognized with mobile-based barcode and divided under each category. By additionally supporting the notification service to let us know about expire date, goods can be efficiently managed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.1C
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• pp.17-23
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• 2012

RFID(Radio Frequency IDentification) is now being applied in the lots of fields as an alternative of barcode system. However, it is restricted within product manufacturing, sales, and delivery parts. In this paper, research results regarding an actual progress of an RFID-based system and its effect are described. In addition, this paper confirms that the RFID-based process of manufacture gives much better return of investment than the barcode-based one. Therefore, the results of this paper are highly expected to contribute to make many people, who might hesitate to replace the barcode and apply RFID system to the process of manufacture, a positive decision.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.45-53
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• 2014

Objectives : Due to the morphological similarity of in the roots of herbal medicine, the official herbal medicine is very difficult to authenticate between the original plants of Patriniae Radix and two adulterant Patrinia species. Therefore, we introduced DNA barcode analysis to establish a powerful tool for the authentication of Patriniae Radix from its adulterants. Methods : To analyze DNA barcode regions, genomic DNA was extracted from twenty-nine specimens of Patrinia scabiosaefolia, Patrinia villosa, Patrinia saniculifolia, and Patrinia rupestris, and internal transcribed spacer 2(ITS2), matK and rbcL genes were amplified. For identification of species specific sequences, a comparative analysis was performed by the ClastalW based on entire sequences of ITS2, matK and rbcL genes, respectively. Results : In comparison of three DNA barcode sequences, we identified 22, 22, and 12 species-specific nucleotides enough to distinguish each four species from ITS2, matK and rbcL gene, respectively. The sequence differences at the corresponding positions were available genetic marker nucleotides to discriminate the correct species among analyzed four species. These results indicated that comparative analysis of ITS2, matK and rbcL genes were useful genetic markers to authenticate Patriniae Radix. Conclusions : The marker nucleotides enough to distinguish P. scabiosaefolia, P. villosa, P. saniculifolia, and P. rupestris, were obtained at 22 SNP marker nucleotides from ITS2 and matK DNA barcode sequences, but they were confirmed at 12 SNP marker nucleotides from rbcL. These differences could be used to authenticate Patriniae Radix from its adulterants as well as discriminating each four species.