Browse > Article
http://dx.doi.org/10.6116/kjh.2013.28.6.53.

Molecular Authentication of Pinelliae Tuber from its adulterants by the analysis of DNA barcodes, matK and rbcL genes  

Lee, Young Mi (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
Moon, Byeong Cheol (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
Ji, Yunui (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
Kim, Wook Jin (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
Kim, Ho Kyoung (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
Publication Information
The Korea Journal of Herbology / v.28, no.6, 2013 , pp. 53-58 More about this Journal
Abstract
Objectives : Pinelliae Tuber has been used as a typical unauthentic herbal medicines. Due to the morphological similarity between Pinelliae Tuber and adulterants, the correct authentication is very difficult. Therefore, we introduced DNA barcode to establish a powerful tool for the authentication of Pinelliae Tuner from adulterants. Methods : To obtain DNA barcode regions, genomic DNA was extracted from nineteen specimens of Pinellia ternata, Pinellia pedatisecta, Pinellia tripartita, and Typhonium flagelliforme, and matK and rbcL genes were amplified. For identification of species specific sequences and analysis phylogenetic relationship, a comparative analysis were performed by the ClastalW and UPGMA based on entire sequences of matK and rbcL genes, respectively. Results : In comparison of two DNA barcode sequences, we elucidated the phylogenetic relationship showing distinct four groups depending on species and identified 40 and 20 species specific nucleotides enough to distinguish each species from matK and rbcL gene, respectively. The sequence differences at the corresponding positions were avaliable genetic marker nulceotides to discriminate the correct species among analyzed four species. These results indicated that phylogentic and comparative analysis of matK and rbcL genes are useful genetic markers to authenticate Pinelliae Tubers. Conclusions : The marker nucleotides enough to distinguish P. ternata, P. tripatrita, P. peditisecta, and T. flagelliform, were observed at 40 positions in matK gene and 20 positions in rbcL gene sequence, respectively. These differences can be used to authenticate Pinelliae Tuber from adulterants as well as discriminate each four species.
Keywords
DNA barcode; Pinelliae Tuber; matK gene; rbcL gene; Molecular Authentication; Marker Nucleotide;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Herbology Editorial Committee of Korean Medicine School. Herbology(Boncho-hak). Seoul : Younglimsa. 2005 : 487-8.
2 Tae KH, Kim DK, Kim JH. Analysis of phylogenetic relationship among Korean Pinellia Tenore (Araceae) using RAPD markers. Korean J Pl Taxon. 2005 ; 35 : 161-74.   과학기술학회마을
3 Korea Food & Drug Administration. The Korean Pharmacopoeia. 10th Edition. Chungwon : KFDA. 2012 : 41-2.
4 Choi YS, Kil KJ, L YJ. A study of morphological identification of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma. Kor J Herbology. 2007 ; 22 : 103-8.   과학기술학회마을
5 Chung HS, Um JY, Kim MS, Hong SH, Kim SM, Kim HK, Park SJ, Kim SC, Hwang WJ, Kim HM. Determination of the site of origin of Pinellia ternata roots based on RAPD analysis and PCR-RFLP. Hereditase. 2002 : 126-9.
6 Liu T, Guo X. Detection of Arisaema yunnanense as adulterant of traditional medicine Pinellia ternata using allele-specific diagnostic polymerase chain reaction (PCR). Afr J Biotechnol. 2010 ; 9 : 8597-601.
7 Song MJ, Kim H. Taxonomic Identities of Cirsium setidens, C. chanroenicum and C. toraiense. Korean J Pl Taxon. 2005 ; 35(4) : 227-45.   과학기술학회마을
8 Guo Y, Ge S. Molecular phylogeny of Oryzeae (Poaceae) based on DNA sequences from chloroplast, mitochondrial, and nuclear genomes. Am J Bot. 2005 ; 92 : 1548-58.   DOI   ScienceOn
9 Cabrera LI, Salazar GA, Chase MW, Mayo SJ, Bogner J, Davila P. Phylogenetic relationships of Aroids and Duckweeds (Araceae) inferred from coding and noncoding plastid DNA. Am J Bot. 2008 ; 95 : 1153-65.   DOI   ScienceOn
10 Kato H, Oginuma K, Gu Z, Hammel B, Tobe H. Phylogenetic relationships of Betulaceae based on matK sequences with particular reference tp the positions of Ostryopsis. APG. 1998 ; 49 : 89-97.
11 Mansion G, Rosenbaum G, Schoenenberger N, Bacchetta G, Rossello JA, Conti E. Phylogenetic analysis informed by geological history supports multiple, sequential invasions of the Mediterranean Basin by the angiosperm family Araceae. Syst Biol. 2008 ; 57 : 269-85.   DOI   ScienceOn
12 Thompson JD, Higgins DG, Gibson JJ, CLUSTAL W. improving the sensitivity of progressive multiple alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res. 1994 ; 11 : 4673-80.
13 Yi TS, Li H, Li DZ. Chromosome variation in the genus Pinellia (Araceae) in China and Japan. Bot J Linn Soc. 2005 ; 147 : 449-55.   DOI   ScienceOn
14 Sang T, Crawford DJ, Stuessy TF. Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am J Bot. 1997 ; 84 : 1120-36.   DOI   ScienceOn
15 Wendel JF. Genome evolution in polyploids. Plant Molecular Biology. 2000 ; 42 : 225-49.   DOI   ScienceOn
16 Stebbines GL. Variation and evolution in plants. New York : Columbia university press. 1971.
17 Olmstead RG, Reeves PA. Evidence for the polyphyly of the Scropulariaceae based on choloroplast rbcL and ndhF sequences. Ann Missouri Bot Gard. 1995 ; 82 : 176-93.   DOI   ScienceOn
18 Grant V. Plant speciation. New York : Columbia university press. 1981.
19 Masterson J. Stomatal size in fossil plants : evidence for polyploidy in majority of angiosperms. Science. 1994 ; 264 : 421-4.   DOI   ScienceOn
20 Lee WK, Kim MJ, Heo K. Phylogeny of Korean Rhus spp. based on ITS and rbcL sequences. Korean J Medicinal Crop Sci. 2004 ; 21 : 60-6.   과학기술학회마을