• 한국콘텐츠학회논문지
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• 제10권9호
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• pp.126-136
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• 2010

본 연구는 일본의 공영방송인 NHK의 역사드라마 콘텐츠가 가져다주는 경제적 효과'에 주목하고 있다. 연구결과, NHK의 역사드라마는 일본의 지역경제 발전에 결정적인 역할을 하는 것으로 나타났다. 일본 은행의 분석에 의하면 2010년 작품인 '료마덴'은 드라마의 무대인 고치현과 나가사키현 등에 무려 6127억원의 경제효과를 가져다주는 것으로 나타났다. 이는 드라마가 큰 인기를 끌면서 지역을 찾는 관광객이 크게 늘어난데 기인한 것이다. NHK는 또 같은 시기에 특정지역을 배경으로 한 드라마 3편을 집중적으로 방송함으로써 해당 지역에 대한 관심도를 부각시키는 전략도 사용하고 있다. NHK의 이런 노력은 제조업계와 서비스업계에도 긍정적인 영향을 미쳤다. 국민이 낸 수신료를 통해 운영되는 NHK는 역사드라마 콘텐츠를 통해 '지역경제발전'을 꾀하는 등 방송의 공공성을 극대화하기 위해 노력하고 있는 것으로 평가되고 있다.

• 대한한방내과학회지
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• 제26권2호
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• pp.333-340
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• 2005

Background: Cancer reseach is done in earnest world-wide, because cancer is one of most threatening diseases to humans. Orostachyos Herba is a widely used herb that has long been in use in Korea as an anti-inflammatory and anti-cancer therapy. The purpose of this study is to verify any anti-cancer effects on stomach and liver cancer in vitro. Materials & Methods: AGS and KATO III stomach cancer cells and Hep3B and HepG2 liver cancer cells, all obtained from Korean Cell Line Bank, were used. The boiled extract of Orostachyos Herba(20 and 40 microliters) were injected into cultures and observed at 0 hours, and at 24-hour intervals up to 96 hours. The destruction of stomach and liver cancer cells was measured through Trypan blue exclusion testing. The suppression on viability of stomach and liver cancer cells was observed, and anti-cancer mechanisms was examined by analyzing the cell cycle. Results: In morphologic change, AGS, KATO III, HepG2 and Hep3B showed some of the withdrawn and floating appearance that is typical in cellular imparment. AGS, KATO III, HepG2 and Hep3B showed more destruction of stomach cancer cells in each test group than in the control group to a statistically significant degree. Analysis of the cell cycle after introduction of Orostachyos Herba showed very little inhibition of divisions of all cell lines. Conclusions: This experiment suggests that Orostachyos Herba has some anti-tumor effects on stomach and liver cancer cells. Progressive research on Orostachyos Herba and it's anti-tumor effects will be needed to determine its practicability as a cancer treatment.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.326-331
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• 2005

A rumen simulated continuous culture (RSCC) system was used to study the influence of supplementation of the three different types of protein sources such as urea, casein and soy protein on rumen microbial synthesis in terms of rumen microbial synchronization. The urea treatment showed the highest pH value. Ammonia nitrogen concentration was rapidly increased after feeding and not significantly different in the urea treatment (13.53 mg/100 ml). Protozoa numbers were not significantly different for soy protein and casein treatment compared to urea treatments during incubation. The average concentration of total VFA (mMol) was not detected with significant difference among treatments, but iso-butyrate production showed the highest for soy protein treatment among treatments (p<0.001). The lowest concentration in total iso-acids (iso-butyrate and iso-valerate) production was observed in urea treatment. The soy protein treatment showed no significantly change in acetate/propionate. The amounts of dry matter (DM) out flow showed no significant difference among treatments. Organic matter (OM) flow was the highest for urea treatments and the lowest for casein treatment (p<0.03). The nitrogen flow for casein treatment was not significantly different from other treatments. The efficiency of microbial protein synthesis in terms of microbial nitrogen (MN) synthesis (g MN/kg ADOM) digested in the rumen was highest for casein treatment (58.53 g MN/kg ADOM) compared to soy protein and urea (p<0.05). This result suggests that rumen ammonia releasing rate may influence on microbial protein synthesis in the rumen.

• 한국방재학회 논문집
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• 제10권5호
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• pp.125-133
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• 2010

일반적으로 사행하천의 만곡부에서는 홍수시 월류의 위험성이 높을 뿐만 아니라, 국부적으로 집중되는 흐름분포는 제방의 안정성을 위협한다. 따라서 본 연구는 두 개의 다른 실험수로에 대한 CCHE2D모형의 적용성 검토와 급변만곡의 형태를 갖는 자연하천의 흐름특성분석을 수행하는데 그 목적이 있다. 모의결과 실험에 대한 수위의 백분율 오차는 4.9%이내였으며, 실측치와 근사한 유속분포를 보였다. 실험수로를 통하여 보정된 모형을 이용하여 용담댐 하류 대유리에 위치한 만곡부를 대상으로 흐름특성 분석을 수행하였다. 모의결과는 만곡부의 지형에 의한 영향을 고려하지 못하는 HEC-RAS모형에 비하여 상류부에서 수위가 1.5 m 정도 높게 나타났다. 그러나 모의결과를 기존의 경험식과 비교한 결과 CCHE2D모형이 편수위 모의에 적합함을 알 수 있었으며, 자연하천의 홍수위와 유속산정에도 적합함을 확인할 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권4호
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• pp.316-322
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• 2004

A low molecular weight component named bone morphogenetic protein(BMP) chemically isolated from the organic matrix of bone, induce postfetal connective tissue cells surrounding small blood vessels to differentiate into cartilage and bone. The end product of BMP is a spherical ossicle of lamella bone filled with red bone marrow for the functional loading. This is a important point that the graft material is embedded the defect site during the implant surgery. Because present knowledge of the relationship between BMP and bone regeneration arises mainly from studies of induced bone formation in heterotopic sites, it would be helpful to determine whether BMP plays any part in the process of bone healing. The BMPs have been shown to play crucial roles in normal skeletal development as well as bone healing and are able to activate transcription of genes involved in cellular migration, proliferation, and differentiation. The delivery of BMP on matrices has been efficacious in the treatment of defect bone in implant surgery. The purpose of the histologic study was to evaluate the effect of DLB(demineralized lyophilized bone) coated with purified human BMP(hBMP-I) in immediate implant surgery with bony defect to obtain the functional structure of implant asap. The ability of a graft of hBMP-I to accelerate bony defect repair provides a rationale for its use in immediate implant surgery that have large bone defect in edentulous area.

• 통상정보연구
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• 제15권2호
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• pp.25-47
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• 2013

중국 중소형 기업들에게 상업은행을 통한 자금 조달은 매우 중요한 자금 조달의 원천이 되고 있다. 그러나 이러한 기업들의 신용보증 부족으로 중국 상업은행은 중소형 기업들에 대한 자금 공여를 꺼리고 있는 실정이며 이러한 현실은 이들 기업의 무역활동에 부정적인 영향을 미칠 수 있을 것이다. 본 연구에서는 중국 중소형 기업들에 대한 신용 보증기관의 위험에 관해 VAR 모델을 적용하여 중국 보증기관의 위험에 영향을 미치는 요인을 분석하고 자 한다. 분석 결과, 기업 규모는 그다지 통계적으로 유의한 요인이 되지 못하였으나 기업의 고정자산 비율 등 담보 관련 정보는 신용보증기관의 VAR를 감소시키는 요인으로 분석되었다. 또한 기업 관련 제품의 독창성 여부 및 중소형 중국기업의 운영위험은 신용보증기관의 VAR와 양의 관계를 보여주고 있다고 분석되었다.

• 재무관리연구
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• 제23권1호
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• pp.135-163
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• 2006

본 연구는 기업공개(IPO)전 은행과의 관계가 최초공모주 가격결정에 어떠한 영향을 미치는가를 실증분석하였다. 본 연구에서는 2001년 1월부터 2003년 9월까지를 대상기간으로 하여 코스닥시장에 신규 상장된 총 343개 기업을 표본으로 삼았다. 미국의 경우를 연구한 James and Wier(l990)의 결과와는 달리 본 논문에서는 우리나라의 경우 기업공개전에 형성된 은행관계가 오히려 기업공개 후 초기 초과수익률의 규모를 증가시키는 역할을 하고 있음을 발견하였다. 또한 은행 및 벤처캐피탈로부터 중복 지원을 받은 기업들의 초기 초과수익률이 가장 높게 나타나고, 두 금융기관의 지원이 전혀 없는 기업들의 초기 초과수익률이 가장 낮게 나타나고 있음을 발견하였다. 이러한 IPO 초기성과는 은행차입금의존도가 높을수록 그리고 청약경쟁률이 높을수록 크게 나타나는 경향이 있었다. 본 연구의 결과는 우리나라 IPO시장에서 기업공개 직후 관찰되는 정(+)의 초기성과는 대부분 과도한 수요로 인하여 높게 형성되는 시장가격 때문이라는 최문수 (1999)의 연구결과와 전반적으로 일치하고 있다.

• Molecules and Cells
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• 제27권3호
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• pp.365-381
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• 2009

The chloroplast DNA sequences of Megaleranthis saniculifolia, an endemic and monotypic endangered plant species, were completed in this study (GenBank FJ597983). The genome is 159,924 bp in length. It harbors a pair of IR regions consisting of 26,608 bp each. The lengths of the LSC and SSC regions are 88,326 bp and 18,382 bp, respectively. The structural organizations, gene and intron contents, gene orders, AT contents, codon usages, and transcription units of the Megaleranthis chloroplast genome are similar to those of typical land plant cp DNAs. However, the detailed features of Megaleranthis chloroplast genomes are substantially different from that of Ranunculus, which belongs to the same family, the Ranunculaceae. First, the Megaleranthis cp DNA was 4,797 bp longer than that of Ranunculus due to an expanded IR region into the SSC region and duplicated sequence elements in several spacer regions of the Megaleranthis cp genome. Second, the chloroplast genomes of Megaleranthis and Ranunculus evidence 5.6% sequence divergence in the coding regions, 8.9% sequence divergence in the intron regions, and 18.7% sequence divergence in the intergenic spacer regions, respectively. In both the coding and noncoding regions, average nucleotide substitution rates differed markedly, depending on the genome position. Our data strongly implicate the positional effects of the evolutionary modes of chloroplast genes. The genes evidencing higher levels of base substitutions also have higher incidences of indel mutations and low Ka/Ks ratios. A total of 54 simple sequence repeat loci were identified from the Megaleranthis cp genome. The existence of rich cp SSR loci in the Megaleranthis cp genome provides a rare opportunity to study the population genetic structures of this endangered species. Our phylogenetic trees based on the two independent markers, the nuclear ITS and chloroplast MatK sequences, strongly support the inclusion of the Megaleranthis to the Trollius. Therefore, our molecular trees support Ohwi's original treatment of Megaleranthis saniculifolia to Trollius chosenensis Ohwi.

• BMB Reports
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• 제49권4호
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• pp.220-225
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• 2016

Cancer cells have different characteristics due to the genetic differences where these unique features may strongly influence the effectiveness of therapeutic interventions. Here, we show that the spontaneous reactivation of extracellular signalregulated kinase (ERK), distinct from conventional ERK activation, represents a potent mechanism for cancer cell survival. We studied ERK1/2 activation in vitro in SW480 colorectal cancer cells. Although ERK signaling tends to be transiently activated, we observed the delayed reactivation of ERK1/2 in epidermal growth factor (EGF)-stimulated SW480 cells. This effect was observed even after EGF withdrawal. While phosphorylated ERK1/2 translocated into the nucleus following its primary activation, it remained in the cytoplasm during late-phase activation. The inhibition of primary ERK1/2 activation or protein trafficking, blocked reactivation and concurrently increased caspase 3 activity. Our results suggest that the biphasic activation of ERK1/2 plays a role in cancer cell survival; thus, regulation of ERK1/2 activation may improve the efficacy of cancer therapies that target ERK signaling.

• Food Science and Biotechnology
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• 제15권2호
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• pp.202-206
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• 2006

The oxidation stability of fish oil containing omega-3 polyunsaturated fatty acids (PUFAs), which is very susceptible to oxidative deterioration, needs to be improved before it can be successfully applied to functional foods. The antioxidant activities of 17 species of materials in anchovy oil (AO) were compared and a potent antioxidant was determined to improve the shelf-life of refined AO. Antioxidant activities of the 0.05% (w/w) materials in AO were compared against control during storage at $30^{\circ}C$ for 10 days. While no antioxidant effect was shown in alpha tocopherol against control, 3 species of grapefruit seed extracts (GSEs), astaxanthin (AX), soybean lecithin, and green tea extract showed good antioxidant activities. Especially, GSE B, GSE C, and AX showed significantly high peroxide inhibitory activities (PIAs) of $16.2{\pm}2.1$, $20.{\pm}3.5$, and $17.7{\pm}3.5%$, respectively, after the 4th day (p<0.01). Radical scavenging activities (RSAs) of GSE B, GSE C, and AX were $85.1{\pm}0.8$, $95.3{\pm}0.3$, and $85.9{\pm}0.8%$, respectively. Correlation between PIAs and RSAs was high ($R^2=0.926$) in GSE B, GSE C, and AX. Therefore, we concluded that one of the main antioxidative mechanisms of GSEs and AX must operate through an RSA pathway. The $RC_{50}$ (concentration required for 50% reduction of 1,1-diphenyl-2-picryl-hydrazyl, DPPH) of GSE C was $258\;{\mu}g/mL$.