• 식물병연구
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• 제26권3호
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• pp.159-169
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• 2020

Pactobacterium carotovorum subsp. carotovorum (Pcc)에 의한 무름병은 전세계적으로 문제가 되고 있고 특히 온난 다습한 지역에서 문제가 심각하다. 본 연구는 Pcc에 의해 발생하는 배추 무름병에 대한 효율적인 저항성 검정법을 확립하기 위하여 실험하였다. 시판 배추 품종 65개의 Pcc KACC 10225에 대한 저항성 정도를 조사하고, 추후 실험을 위해 저항성에 차이를 보이는 3개 품종을 선발하였다. 이들 3개 품종의 접종하는 배추의 생육 시기, 접종원 농도, 접종 후 재배 온도 등의 발병 조건에 따른 무름병 발생을 조사하였다. 이들 실험의 결과로부터 배추의 무름병에 대한 저항성을 검정하기 위해서는, 배추 종자를 파종하고 온실(25±5℃)에서 21일 동안 재배한 유묘에 Pcc 균주의 세균현탁액(1×10⁷ cfu/ml)을 식물체 기부에 5 ml씩 관주하여 접종하고, 접종한 식물은 25℃ 습실상에 24시간 동안 배양한 후에 25℃, 상대습도 80%의 생육상으로 이동하여 재배하는 것을 제안하고자 한다.

• 농약과학회지
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• 제13권4호
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• pp.325-331
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• 2009

세 종류의 유용세균, Lactobacillus KLF01, Lactococcus KLC02 그리고 Paenibacillus KPB3는 무름병원세균 Pectobacterium carotovorum subsp. carotovorum (Pcc)에 대한 기내 길항효과를 나타내었으며, 온실 내 포트에서 배추 무름병에 대한 생물적 방제 효과를 병원균 접종 4, 8, 12일 후 각각 조사한 결과 KLC02 균주는 64%, 50%, 56%, KPB3 균주는 66%, 57%, 58%로 나타났다. 반면, KLF01 균주는 KLC02 및 KPB3 균주에 비해 그 효과가 다소 낮았으나 유의성이 인정될 수 있는 방제효과로 조사되었다. 또한 재배포장에서 배추 무름병에 대한 생물적 방제효과는 KLF01, KLC02 및 KPB3 모두 각각 55%, 60% 그리고 62%로 매우 효과적임을 알 수 있었다. 따라서 세 종류의 유용세균 Lactobacillus KLF01, Lactococcus KLC02 그리고 Paenibacillus KPB3 균주를 이용하여 배추 무름병을 억제하기 위한 생물적 방제제로 활용할 수 있음을 보고한다.

• The Plant Pathology Journal
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• 제35권2호
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• pp.178-187
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• 2019

Plants are exposed to biotic stresses caused by pathogen attack and complex abiotic stresses including heat and drought by dynamic climate changes. To alleviate these stresses, we investigated two bacterial stains, H26-2 and H30-3 in two cultivars ('Ryeokkwang' and 'Buram-3-ho') of Chinese cabbage in plastic pots in a greenhouse. We evaluated effects of bacterial strains on plant growth-promotion and mitigation of heat and drought stresses; the role of exopolysaccharides as one of bacterial determinants on alleviating stresses; biocontrol activity against soft rot caused by Pectobacterium carotovorum subsp. carotovorum PCC21. Strains H26-2 and H30-3 significantly increased fresh weights compared to a $MgSO_4$ solution; reduced leaf wilting and promoted recovery after re-watering under heat and drought stresses. Chinese cabbages treated with H26-2 and H30-3 increased leaf abscisic acid (ABA) content and reduced stomatal opening after stresses treatments, in addition, these strains stably colonized and maintained their populations in rhizosphere during heat and drought stresses. As well as tested bacterial cells, exopolysaccharides (EPS) of H30-3 could be one of bacterial determinants for alleviation of tested stresses in Chinese cabbages, however, the effects were different to cultivars of Chinese cabbages. In addition to bacterial activity to abiotic stresses, H30-3 could suppress incidence (%) of soft rot in 'Buram-3-ho'. The tested strains were identified as Bacillus aryabhattai H26-2 and B. siamensis H30-3 based on 16S rRNA gene sequence analysis. Taken together, H26-2 and H30-3 could be candidates for both plant growth promotion and mitigation of heat and drought stresses in Chinese cabbage.

• 식물병연구
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• 제29권1호
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• pp.64-71
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• 2023

In October 2021, soft rot disease seriously affected radish crop in Dangjin, Chungcheongnam-do, Korea. The infected radishes were stunted and turned dark green, with yellowish leaf foliage. A slimy, wet, and decayed pith region was observed in the infected roots. The bacterial strain KNUB-03-21 was isolated from infected roots. The biochemical and morphological characteristics of the isolate were similar to those of Pectobacterium brasiliense. Phylogenetic analysis based on the sequences of the 16S rRNA region and the concatenated DNA polymerase III subunit tau (dnaX), leucine-tRNA ligase (leuS), and recombinase subunit A (recA) genes confirmed that the isolate is a novel strain of P. brasiliense. Artificial inoculation of radish with P. brasiliense KNUB-03-21 resulted in soft rot symptoms similar to those observed in infected radish in the field; subsequently, P. brasiliense KNUB-03-21 was reisolated and reidentified. To our knowledge, this is the first report of P. brasiliense as a causal pathogen of radish soft rot in Korea.

• 식물병연구
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• 제29권4호
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• pp.445-451
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• 2023

Zamioculcas zamiifolia is a popular indoor ornamental plant in Korea. In August 2021, a severe outbreak of soft rot disease affected Z. zamiifolia in Emseong, Chungcheongbuk-do, Korea. Infected plants displayed wilting, water-soaked lesions, stem collapse, and green-brown discoloration. The bacterial strain KNUB-05-21 was isolated from infected stems and identified as Pectobacterium aroidearum using 16S rRNA nucleotide sequencing and multilocus sequence analysis based on partial sequences of dnaX, leuS, and recA genes. Confirmation of its affiliation with P. aroidearum was also obtained through biochemical and morphological characterization. To confirm the pathogenicity of strain KNUB-05-21, its suspension was injected into Z. zamiifolia stems. Within a week, soft rot developed on the stems, exhibiting symptoms similar to those observed in field-infected plants. The reisolated strain was identical to those of P. aroidearum. Before this study, P. aroidearum was not reported as a causative pathogen of Z. zamiifolia soft rot in Korea.

• 식물병연구
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• 제29권4호
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• pp.452-458
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• 2023

In the Goesan region, located in Chungcheongbuk-do, Korea, a significant outbreak of soft rot infections was documented in August 2021, affecting fruits of Momordica charantia, commonly known as bitter melon or bitter gourd. The symptoms included a noticeable transition to yellowing in the affected fruits, eventually leading to their collapse. The bacterial strain KNUB-09-21 was isolated from the diseased fruits. Molecular analysis, using the sequences of the 16S rRNA region and three housekeeping genes (dnaX, recA, and leuS), along with the results of compound utilization in the API ID 32 GN system, provide strong evidence for the identification of the isolate KNUB-09-21 as Pectobacterium brasiliense. The pathogenicity of strain KNUB-09-21 on M. charantia was confirmed through a controlled inoculation test. Within two days, inoculated fruits displayed soft rot symptoms closely resembling those observed in naturally affected fruits. This is the first report of soft rot on M. charantia in Korea.

• The Plant Pathology Journal
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• 제17권6호
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• pp.362.1-362
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• 2001

• 생물환경조절학회지
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• 제6권4호
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• pp.317-323
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• 1997

Erwinia rarotovora subsp carotovora에 의한 콩나물 무름병 발생을 인위적인 조건하에서 조사하였다. 콩나물의 재배 온도가 콩나물 무름병 발생과 생장에 미치는 영향은 3$0^{\circ}C$이상의 고온에서 무름병 발생율이 높아졌으며 35$^{\circ}C$에서는 약 22%의 발병율을 나타내었다. 종피와 자엽 부위에 인위적인 상처를 낸 경우 70%이상의 발병율을 보였으며 콩나물의 신장을 억제하였다. 습도는 90%이상 유지시 33%의 발병율을 보였으며 콩나물의 생장을 억제시켰다. 콩나물 재배 배출액을 관수액으로 재이용하였을 때 무름병의 발병율이 증가하였다.

• 식물병연구
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• 제30권1호
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• pp.95-98
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• 2024

In a survey conducted in March 2023, Euphorbia hypogaea plants cultivated within greenhouses in Yongin, Korea exhibited water-soaked areas near the stem base, close to the soil. Subsequent isolation from diseased E. hypogaea led to the identification of a bacterial strain, designated as CBNUMPBL-103. The isolate was identified as Dickeya dadantii through sequencing of the 16s rRNA and phylogenetic analysis. The pathogenicity of the isolate was confirmed by inoculating it into healthy E. hypogaea, resulting in the manifestation of similar symptoms observed during the survey. The re-isolated strain recovered from inoculated plants showed a similar morphology with the inoculated strain. This is the first documentation of D. dadantii causing soft rot of E. hypogaea in Korea.

• 식물병연구
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• 제6권1호
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• pp.6-9
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• 2000

1998년 수원, 성남, 양재, 대전의 비닐하우스에서 재배되고 있는 인삼고무나무(Ficus retusa), 황금대만고무나무(Ficus retusa \`Golden leaf\`), 벤자민고무나무(Ficus benjamina)에 새로운 세균병이 관찰되었다. 인삼고무나무의 뿌리에 무름증상을 보이는 세균병을 \"P.cichorii\"에 의한 인삼고무나무의 세균성뿌리썩음병\", 황금대만고무나무와 벤자민고무나무에 마름증상을 보이는 세균병을 \"P.viridiflava에 의한 황금대만고무나무와 벤자민고무나무의 세균성잎마름병\"으로 명명할 것을 제안한다.마름병\"으로 명명할 것을 제안한다.제안한다.