• 한국미생물·생명공학회지
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• 제26권6호
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• pp.471-475
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• 1998

신생아 분변으로부터 장내세균 127균주를 분리하였다. 각각의 균주들의 배양균체를 검정종양세포들에 대해 항종양활성을 측정한 결과, 항종양활성을 나타내는 분리균주는 27균주였고, 그 중 비교적 강한 항종양활성을 보이는 균주는 5균주였으며, 5균주중 위암세포주, SNU-1에 선택적으로 강한 항종양활성을 나타내는 2B4-1 균주를 최종분리하였다. API 20 S system kit를 이용하여 분리 균주 2B4-1을 동정한 결과, Enterococcus faecalis와 99.9%의 % ID value를 나타내었다. 동정된 분리균주 2B4-1과 type strain, Enterococcus faecalis NCTC 775와의 생리생화학적 특성들을 비교해 본 결과, 온도별대 성장률, 내염성, 내산성, arabinose등의 당 이용성 및 통성혐기 상태에서의 생육 등에서는 일치된 결과를 나타내었으나 hippurate hydrolysis test의 음성반응 및 $\beta$-hemolysis test의 음성반응을 나타낸 것으로 보아 Enterococcus faecalis NCTC 775와 2가지의 다른 특성을 나타내었다. 따라서 분리균주 2B4-1은 Enterococus faecalis NCTC 775와는 약간의 특성이 다른 변종으로 확인되어 Enterococcus faecalis 2B4-1으로 동정하였다.

• Molecules and Cells
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• 제20권3호
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• pp.392-400
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• 2005

The genes encoding the DNA gyrase A (GyrA) and B subunits (GyrB) of Methylovorus sp. strain SS1 were cloned and sequenced. gyrA and gyrB coded for proteins of 846 and 799 amino acids with calculated molecular weights of 94,328 and 88,714, respectively, and complemented Escherichia coli gyrA and gyrB temperature sensitive (ts) mutants. To analyze the role of type II topoisomerases in the intrinsic quinolone resistance of methylotrophic bacteria, the sequences of the quinolone resistance-determining regions (QRDRs) in the A subunit of DNA gyrase and the C subunit (ParC) of topoisomerase IV (Topo IV) of Methylovorus sp. strain SS1, Methylobacterium extorquens AM1 NCIB 9133, Methylobacillus sp, strain SK1 DSM 8269, and Methylophilus methylotrophus NCIB 10515 were determined. The deduced amino acid sequences of the QRDRs of the ParCs in the four methylotrophic bacteria were identical to that of E. coli ParC. The sequences of the QRDR in GyrA were also identical to those in E. coli GyrA except for the amino acids at positions 83, 87, or 95. The $Ser^{83}$ to Thr substitution in Methylovorus sp. strain SS1, and the $Ser^{83}$ to Leu and $Asp^{87}$ to Asn substitutions in the three other methylotrophs, agreed well with the minimal inhibitory concentrations of quinolones in the four bacteria, suggesting that these residues play a role in the intrinsic susceptibility of methylotrophic bacteria to quinolones.

• 한국식품영양과학회지
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• 제28권3호
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• pp.526-533
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• 1999

Two species of antimicrobial agent producing bacteria and one sensitive strain were isolated from soil. Those were identified as B. subtilis, B. licheniformis, and Curtobacterium sp. by morphological, biochemical, physiological and chemotaxonomic characteristics. These were designated as B. subtilis cx1, B. licheniformis cy2 and Curtobacterium sp. cf3, respectively. Antimicrobial agent produced by B. subtilis cx1 showed high antibacterial activity against gram positive bacteria including of B. subtilis, Curtobacterium sp., L. mesenteroids, Staphy. aureus, S. faecalis and even gram negative bacteria, P. aeruginosa. Antimicrobial agent from B. licheniformis cy2 showed slightly lower antimi crobial activity than that from B. subtilis cx1. These two strains showed maximum production of antimicrobial agents at 30oC for 9~21hr cultivation. Curtobacterium sp. cf3 showed more sensitive activity than a sensitive strain of B. subtilis ATCC 6633 which was same genus or species with the B. subtilis cx1 and B. subtilis cy2, when the antimicrobial agent producing strains, B. subtilis cx1 and B. subtilis cy2, were directly applied onto these sensitive lawns.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• KSBB Journal
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• 제18권6호
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• pp.490-494
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• 2003

토양으로부터 분리한 균주 중 항진균 활성이 우수한 균주를 선발하여 B-1이라 명명하였다. B-1의 항균성 물질 생산에 대한 배양조건을 검토하기 위해 최적배지, 탄소원, 질소원, pH, 온도별로의 영향을 조사하였다. PDB 배지에서 항균력이 우수하였으며 탄소원$.$질소원에는 별다른 영향이 없었다. pH와 온도에서는 각각 pH 5.0, 28$^{\circ}C$에서 항균력이 우수하였다. 항균 활성은 진균에서 강하게 나타났으며 일부 세균에서도 항균력을 나타내었다. 항진균성 물질은 산, 알칼리 처리 후 잔존 활성은 70∼80%로 나타났으며 열처리시 완전히 실활되어 열에 대해 상당히 불안정하다는 것을 확인하였다. 항균성 물질위 용매 전용성을 위해 TLC를 통하여 분석하였으며 plate assy를 실시하여 항균성물질을 확인하였다. 항진균성 물질의 대량생산을 위해 기본배지인 PDB를 직접 실험실 내에서 제조하였으며 시판의 PDB와 비교하였을때 항균성 물질 생산면에서나 가격면에서 더 나은 결과를 보였다.

• 한국응용과학기술학회지
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• 제39권5호
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• pp.605-613
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• 2022

Flavobacterium 속(genus)은 Bacteriodetes 문(phylum), Flavobacteriaceae 과(family)의 대표속(type genus)으로서, 이 속의 세균들은 황색 색소를 함유하는 그람 음성 간균이다. 이 속 세균들은 자연계의 다양한 환경에서 분리되고 있다. 황색색소를 함유하는 그람음성 간균이 경남 창원시 소재 창원대학교 교내의 연못에서 분리되었고, 이 세균은 균주 B1으로 명명되었다. 균주 B1을 생리학적, 생화학적 및 계통분석학적으로 분석한 결과, Flavobacterium 속에 속하는 것으로 결론지어졌다. 이 세균의 16S rRNA 유전자 염기서열을 BLAST로 분석해 본 결과, 다른 어떠한 세균과도 16S rRNA 유전자 염기서열의 상동성이 99.0%를 넘지 않았다. 균주 B1의 주된 지방산은 iso-C_15:0(19.6%), summed feature 3(C_16:1 ω7c and/or C_16:1 ω6c, 16.1%), iso-CC_17:0 3OH(10.2%), iso-C_15:0 3OH(8.4%) 및 iso-C_15:1 G(6.6%)인 것으로 밝혀졌는데, 이는 다른 Flavobacterium 종들의 지방산 함량과 확연한 차이가 있는 것을 알 수 있었다. 이 세균의 16S rDNA 염기서열은 genbank에 accession number OP060681로 등록되었다.

• 한국응용과학기술학회지
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• 제40권4호
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• pp.881-889
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• 2023

Hymenobacter 속(genus)은 Bacteroidota 문(phylum), Hymenobacteraceae 과(family)의 대표 속(type genus)이다. 이 속에 속하는 세균들은 붉은색 색소를 함유하는 그람 음성 간균으로서, 자연계의 다양한 환경에서 분리되고 있다. 본 연구에서 붉은색 색소를 함유하는 그람 음성 간균이 경남 창원시 소재 창원대학교 교내의 연못에서 분리되었고, 이 세균은 균주 B2로 명명되었다. 균주 B2를 계통분석 및 생화학적으로 분석한 결과, Hymenobacter 속에 속하는 것으로 밝혀졌다. 이 세균의 16S rRNA 유전자 염기서열을 genbank의 BLAST로 분석해 본 결과, 다른 어떠한 세균과도 16S rRNA 유전자 염기서열의 상동성이 새로운 미생물로 인정되는 기준인 98.7%보다 낮은 것으로 나타났다. 균주 B2의 지방산을 분석해 본 결과, 주된 지방산은 summed feature 3(C_16:1 ω7c and/or C_16:1 ω6c, 22.8%), iso-C_15:0(16.2%), anteiso-C_15:0(12.9%), C_16:1ω5c(12.4%) 및 summed feature 4 (iso-C_17:1 I/anteiso-C_17:1)(9.5%)인 것으로 밝혀졌는데, 결과적으로 균주 B2의 지방산 함량은 다른 Hymenobacter 종들의 지방산 함량과 뚜렷한 차이가 있는 것을 알 수 있었다. 이 세균의 16S rRNA 유전자 염기서열은 genbank에 accession number OQ318247로 등록되었다.

• 약학회지
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• 제48권1호
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• pp.70-74
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• 2004

Bacillus polyfermenticus SCD which is commonly called as Bisroot (equation omitted) has been appropriately used for the treatment of long-term intestinal disorder's. This strain strongly inhibited against methicillin resistance Staphylococcus aureus (MRSA) and various pathogenic microorganisms. Effects of B. polyfermentius SCD administration on death rates and egg-laying rates in two groups of hens were investigated. This strain decreased the death rates of two groups by 16.26％ and 11.72％, respectively. Also this strain increased the egg-laying rates of those 2.74％ and 2.66％, respectively Clinical tests of B. polyfermenticus SCD administration to healthy adults showed not adverse effects but decreased glyceride concentration from 154.52 mg/dl to 135.41 mg/dl after two week administrations.

• 동아시아식생활학회지
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• 제14권5호
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• pp.508-512
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• 2004

In other to produce vinegar using Schizandra chinensis Ballon(omija), acetic acid bacteria(AAB) were selected from several conventional vinegars, and total 30 acetic acid bacterial strains were isolated. Among the isolated strains, a strain was selected from medium containing omija juice which showed the highest productivity of acetic acid. The strain was identified as Acetobacter sp. C5-1b. Optimum conditions for acetic acid production of Acetabacter sp. C5-1b were involved with 30t: of fermentation temperature and shaking culture. The acidity of culture medium was reached to 5.3% after 8 days shaking cultivation at 30℃.

• The Plant Pathology Journal
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• 제38권5호
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• pp.449-460
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• 2022

This study was performed to reveal phenotypic characters and identity of symbiont bacteria of Nasutitermes as well as investigate their potential as antagonist of plant pathogenic fungi. Isolation of the symbiont bacteria was carried out from inside the heads and the bodies of soldier and worker termite which were collected from 3 locations of nests. Identification was performed using phenotypic test and sequence of 16S ribosomal DNA (16S rDNA). Antagonistic capability was investigated in the laboratory against 3 phytopathogenic fungi i.e., Phytophthora capsici, Ganoderma boninense, and Rigidoporus microporus. Totally, 39 bacterial isolates were obtained from inside the heads and the bodies of Nasutitermes. All the isolates showed capability to inhibit growth of P. capsici, however, 34 isolates showed capability to inhibit growth of G. boninense and 32 isolates showed capability to inhibit growth of R. microporus. Two bacterial strains (IK3.1P and 1B1.2P) which showed the highest percentage of inhibition were further identified based on their sequence of 16S rDNA. The result showed that 1K3.1P strain was placed in the group of type strain and reference strains of Lysinibacillus fusiformis meanwhile 1B1.2P strain was grouped within type strain and reference strains Paenibacillus alvei. The result of this study supply valuable information on the role of symbiont bacteria of Nasutitermes, which may support the development of the control method of the three above-mentioned phytopathogenic fungi.