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Hazard Analysis of Staphylococcus aureus in Ready-to-Eat Sandwiches (즉석섭취 샌드위치류의 황색포도상구균에 대한 위해분석)

  • Park, Hae-Jung;Bae, Hyun-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.7
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    • pp.938-943
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    • 2007
  • This study investigated the hazard analysis of ready-to-eat sandwiches sold in various establishments. Sandwich samples were collected from convenience stores, discount stores, sandwich chain stores, bakery shops, fast-food chain stores, and food service operations located in Daegu and Gyeongbuk. Out of 174 samples, 18 (10.3%) contained coagulase positive staphylococci with counts ranging from 0.30 to 4.08 log CFU/g. There was significant seasonal difference in Staphylococcus aureus isolation; the average count in summer (3.24 log CFU/g) was 3 times higher than that of winter (1.10 log CFU/g) (P<0.001). According to the microbiological guidelines of PHLS for ready-to-eat foods, 95.4% of the samples were acceptable. As a result of enterotoxin producing experimental data ($35^{\circ}C$, pH 5.8, NaCl 0.5%), enterotoxin was not produced in a sandwich until Staphylococcus aureus increased to a level greater than 4.95 log CFU/g. This microbiological hazard analysis data could be applied to future studies on quantitative risk assessment of ready-to-eat foods.

Differential Expression of Glucose Transporter Gene in Mouse Early Embryos (생쥐 초기배아의 Glucose Transporter유전자 발현 양상에 관한 연구)

  • Youm, Hye-Won;Byun, Hye-Kyung;Song, Gyun-Ji;Kim, Hae-Kwon;Lee, Ho-Joon
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.1
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    • pp.77-86
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    • 1998
  • The uptake of glucose for metabolism and growth is essential to most animal cells and is mediated by glucose-transporter (GLUT) proteins. The aim of this study was to determine which class of glucose transporter molecules was responsible for uptake of glucose in the mouse early embryo and at which stage the corresponding genes were expressed. In addition, co-culture system with vero cell was used to investigate the effect of the system on GLUT expression. Two-cell stage embryos were collected from the superovulated ICR female and divided into 3 groups. As a control, embryos were cultured in 0.4% BSA-T6 medium which includes glucose. For the experimental groups, embryos were cultured in either co-culture system with vero cells or glucose-free T6 medium supplemented with 0.4% BSA and pyruvate as an energy substrate. 2-cell to blastocyst stage embryos in those groups were respectively collected into microtubes (50 embryos/tube). Total RNA was extracted and RT-PCR was performed. The products were analysed after staining ethidium bromide by 2% agarose gel electrophoresis. Blastocysts were collected from each group at l20hr after hCG injection. They were fixed in 2.5% glutaraldehyde, stained with hoechst, and mounted for observation. In control, GLUT1 was expressed from 4-cell to blastocyst. GLUT2 and GLUT3 were expressed in morula and blastocyst. GLUT4 was expressed in all stages. When embryos were cultured in glucose-free medium, no significant difference was shown in the expression of GLUT1, 2 and 3, compared to control. However GLUT4 was not expressed until morular stage. When embryos were co-cultured with vero cell, there was no significant difference in the expression of GLUT1, 2, 3 and 4 compared to control. To determine cell growth of embryos, the average cell number of blastocyst was counted. The cell number of co-culture ($93.8{\pm}3.1$, n=35) is significantly higher than that of control and glucose-free group ($76.6{\pm}3.8$, n=35 and $68.2{\pm}4.3$, n=30). This study shows that the GLUT genes are expressed differently according to embryo stage. GLUTs were detectable throughout mouse preimplantation development in control and co-culture groups. However, GLUT4 was not detected from 2- to 8-cell stage but detected from morula stage in glucose-free medium, suggested that GLUT genes are expressed autocrinally in the embryo regardless of the presence of glucose as an energy substrate. In addition, co-culture system can increase the cell count of blastocyst but not improve the expression of GLUT. In conclusion, expression of GLUT is dependent on embryo stage in preimplantation embryo development.

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An Optimization of Hashing Mechanism for the DHP Association Rules Mining Algorithm (DHP 연관 규칙 탐사 알고리즘을 위한 해싱 메커니즘 최적화)

  • Lee, Hyung-Bong;Kwon, Ki-Hyeon
    • Journal of the Korea Society of Computer and Information
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    • v.15 no.8
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    • pp.13-21
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    • 2010
  • One of the most distinguished features of the DHP association rules mining algorithm is that it counts the support of hash key combinations composed of k items at phase k-1, and uses the counted support for pruning candidate large itemsets to improve performance. At this time, it is desirable for each hash key combination to have a separate count variable, where it is impossible to allocate the variables owing to memory shortage. So, the algorithm uses a direct hashing mechanism in which several hash key combinations conflict and are counted in a same hash bucket. But the direct hashing mechanism is not efficient because the distribution of hash key combinations is unvalanced by the characteristics sourced from the mining process. This paper proposes a mapped perfect hashing function which maps the region of hash key combinations into a continuous integer space for phase 3 and maximizes the efficiency of direct hashing mechanism. The results of a performance test experimented on 42 test data sets shows that the average performance improvement of the proposed hashing mechanism is 7.3% compared to the existing method, and the highest performance improvement is 16.9%. Also, it shows that the proposed method is more efficient in case the length of transactions or large itemsets are long or the number of total items is large.

Architecture Design of High Performance H.264 CAVLC Encoder Using Optimized Searching Technique (최적화된 탐색기법을 이용한 고성능 H.264/AVC CAVLC 부호화기 구조 설계 기법)

  • Lee, Yang-Bok;Jung, Hong-Kyun;Kim, Chang-Ho;Myung, Je-Jin;Ryoo, Kwang-Ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.431-435
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    • 2011
  • This paper presents optimized searching technique to improve the performance of H.264/AVC. The proposed CAVLC encoder uses forward and backward searching algorithm to compute the parameters. By zero-block skipping technique and pipelined scheduling, the proposed CAVLC encoder can obtain better performance. The experimental result shows that the proposed architecture needs only 66.6 cycles on average for each $16{\times}16$ macroblock encoding. The proposed architecture improves the performance by 13.8% than that of previous designs. The proposed CAVLC encoder was implemented using VerilogHDL and synthesized with Megnachip $0.18{\mu}m$ standard cell library. The synthesis result shows that the gate count is about 15.6K with 125Mhz clock frequency.

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An Intra Prediction Hardware Architecture Design for Computational Complexity Reduction of HEVC Decoder (HEVC 복호기의 연산 복잡도 감소를 위한 화면내 예측 하드웨어 구조 설계)

  • Jung, Hongkyun;Ryoo, Kwangki
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.5
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    • pp.1203-1212
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    • 2013
  • In this paper, an intra prediction hardware architecture is proposed to reduce computational complexity of intra prediction in HEVC decoder. The architecture uses shared operation units and common operation units and adopts a fast smoothing decision algorithm and a fast algorithm to generate coefficients of a filter. The shared operation unit shares adders processing common equations to remove the computational redundancy. The unit computes an average value in DC mode for reducing the number of execution cycles in DC mode. In order to reduce operation units, the common operation unit uses one operation unit generating predicted pixels and filtered pixels in all prediction modes. In order to reduce processing time and operators, the decision algorithm uses only bit-comparators and the fast algorithm uses LUT instead of multiplication operators. The proposed architecture using four shared operation units and eight common operation units which can reduce execution cycles of intra prediction. The architecture is synthesized using TSMC 0.13um CMOS technology. The gate count and the maximum operating frequency are 40.5k and 164MHz, respectively. As the result of measuring the performance of the proposed architecture using the extracted data from HM 7.1, the execution cycle of the architecture is about 93.7% less than the previous design.

Bacteriological Contamination of Water in Han River basin (한강유역의 수중미생물 오염도 조사)

  • Choi, Han-Young;Park, Chung-Oh
    • Journal of environmental and Sanitary engineering
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    • v.4 no.2 s.7
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    • pp.47-59
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    • 1989
  • In order to investigate the bacteriological contamination of water in Han river, the survey was carried out in eight reservoirs of Seoul water supply during the period from January to December in 1985. 1. The counts by means of total bacteria in eight reservoirs by standard plate count method were as follows: $7.7\times10^2$ per ml in Paldang reservior, $9.6\times10^3$ per ml in Gueiri, $8.4\times10^4$ per ml in Doogdo, $1.6\times10^6$ per ml in Bogwang, $2.5\times10^6$ per ml in Noryangjin, $2.2\times10^6$per ml in Seon yoo, $5.9\times10^6$ per ml in Yungdeungpo and $1.9\times10^7$per ml in Gayang. 2. The average counts of total coliform in eight reservoirs by MPN method were as follows : $2.4\times10$ per 100 ml in Paldang, $5.6\times10^2$ per 100 ml in Gueiri, $2.3\times10^3$ per 100 ml in Doogdo, $5.1\times10^4$ per 100 ml in Noryang-jin, $1.2\times10^5$ per 100 ml in Bogwang, $6.2\times10^4$ per 100 ml in Seonyoo, $1.1\times10^5$ per 100 ml in Yungdeungpo and $2.8\times10^5$ per 100 ml Gayang. 3. The counts by means of fecal coliform in eight reservoirs by MPN method were as follows : non detection per 100 ml in Paldang, 5.2 per 100 ml in Gueiri, $1.2\times10^2$ per 100 ml in Doogdo, $1.6\times10^3$ per 100ml in Bogwang, $2.0\times10^3$per 100ml in Noryangjin, $6.6\times10^2$ per 100ml in Seonyoo, $1.2\times10^3$ per 100 ml in Yungdeungpo and $2.5\times10^3$per 100 ml in Gayang. 4. The counts by means of fecal streptococci in eight reservoirs by MPN method were as follows: non detection per 100 ml in Paldang and Gueiri, $6.9\times10$ per 100 ml in Doogdo, $3.2\times10^2$ 102 per 100 ml in Bogwang, $2.9\times10^2$ per 100 ml in Noryangjin, $3.0\times10^2$ per 100 ml in Seonyoo, $4.0\times10^2$ per 100 ml in Yungdeungpo and $14\times10^3$ per 100 ml in Gayang. 5. The counts means of pseudomonas aeruginosa in eight reservoirs by MPN method were as follows; non detection per 100 ml in Paldang, 2.4 per 100 ml in Gueiri, $1.5\times10$ per 100 ml in Doogdo, $2.0\times10$ per 100ml in Bogwang,$6.2\times10$ per 100mI in Noryangjin, $2.1\times10$ per 100ml in Seonyoo, $6.4\times10$ per 100mI in Yungdeungpo and $7.1\times10$ per 100ml in Gaynag.

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Supplement of Conjugated Linoleic Acid Increases Neutrophil Phagocytosis in Pigs (Conjugated Linoleic Acid를 급여한 돼지의 호중구 탐식능 증강)

  • Sang-chul, Han;Ji-houn, Kang;Sung-mok, Son;Chung-soo, Chung;Chul-young, Lee;Mhan-pyo, Yang
    • Journal of Veterinary Clinics
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    • v.20 no.4
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    • pp.437-442
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    • 2003
  • To examine the in vivo immunostimulating effect of conjugated linoleic acid (CLA) in pigs, the change of peripheral blood cells and the phagocytic response of phagocytes were evaluated. Spayed male pigs, 80 kg of average body weight, fed a diet containing either 0.5% 10t-12c CLA or 0.5% CLA mixture (mostly 9c-11t CLA and 10t-12c CLA) for 4 weeks. The change of blood cell values (PCV, WBC, differential count of WBC) and the phagocytic activities of phagocytes were evaluated on week 0, 2, 4, and 5, respectively. There were no change in the PCV values regardless of CLA supplement. The number of WBC, especially neutrophils, in pigs fed a diet with CLA was significantly increased (p<0.05 to 0.01) when compared with control pigs fed a diet without CLA. The phagocytosis of peripheral blood mononuclear cell (MNC) and peripheral blood polymorphonuclear cells (PMN) were analyzed by a flow cytometry system. There was no change in the phagocytic activity of MNC and monocyte-rich cells regardless of CLA supplement. However, the phagocytic activity of PMN composed by approximately 95% neutrophils was remarkably increased (p < 0.05 to 0.01) on week 2, 4, and 5 as compared wth control pigs. These results suggested that supplement of CLA into pigs induces the increase of neutrophil number and the enhancement of neutrophil phagocytosis.

Effects of IgY Supplementation on Hanwoo Calves Fed withHigh Quality Roughage (양질 조사료 급여에 따른 한우 송아지의 IgY 처리효과)

  • Hong, Byung-Cheon;Shin, Jong-Suh;Park, Byung-Ki;Kim, Byong-wan;Sung, Kyung-Il;Ahn, Jung-Sang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.2
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    • pp.159-166
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    • 2016
  • This study was conducted to examine the effect of IgY supplementation on growth performance, blood metabolism, and disease occurrence in the weaned calves at 3 or 4 month of age. Average daily gain (ADG) and feed intakes were not affected by IgY supplementation, regardless of weaning months. White blood cell (WBC) count of the control group was higher in the final stage than in the initial stage (p<0.05), while that of the IgY supplemented group was lower in the final stage compared to the initial stage (p<0.05). regardless of treatments. Hematocrit (HCT) and red blood cell (RBC) counts were lower in the final stage than in the initial stage (p<0.05), but mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) counts were higher in the final stage than in the initial stage, regardless of treatments. In the control group, concentrations of albumin and globulin were higher in the final stage compared to the initial stage in the weaned calves at 3 month of age (p<0.05), and concentration of blood urea nitrogen (BUN) was increased in the IgY supplemented group. In the control group, concentrations of aspartate aminotransferase (AST) and gamma (${\gamma}$)-glutamyl transferase (GGT) were higher in the final stage compared to the initial stage (p<0.05). In contrast, concentrations of aspartate aminotransferase (AST) and gamma(${\gamma}$)-glutamyl transferase (GGT) were lower in the final stage compared to the initial stage for the IgY supplemented group. A diarrhea calf was only found in the control group. Manure score was higher in the control group compared to IgY supplemented group (p<0.05). Thus, these results indicates that IgY supplementation had positive effects on some blood metabolites, fecal condition score and diarrhea without negative effect on growth performance of the weaned Hanwoo calves.

Studies on the Physicochemical Characteristics of Traditional Kochujang (전통 고추장의 품질특성)

  • Shin, Dong-Hwa;Kim, Dong-Han;Choi, Ung;Lim, Dae-Kwan;Lim, Mi-Sun
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.157-161
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    • 1996
  • Physicochemical and microbial characteristics of traditional kochujang (fermented hot pepper-soybean paste) collected from 55 households at different regions were investigated. The traditional kochujang contained $46.71{\pm}5.98%$ moisture, $46.87{\pm}8.83%$ total sugar, $11.77{\pm}3.90%$ crude protein, $15.01{\pm}6.48%$ salt, $27.52{\pm}7.32%$ reducing sugar, $0.26{\pm}0.15%$ amino nitrogen and $2.69{\pm}2.35%$ ethanol. The pH and titrable acidity were $4.60{\pm}0.23$ and $27.26{\pm}10.98\;ml/10\;g$, respectively. The average water activity of traditional kochujang were $0.79{\pm}0.04$. The Hunter L, a, and b values of kochujang were $16.03{\pm}2.89$, $20.42{\pm}4.37$, and $9.71{\pm}1.92$, respectively. The viable cell counts of aerobic, anaerobic bacteria and yeasts in the traditional kochujang were $1.02{\times}10^8{\pm}1.29{\times}10^8\;CFU/g$, $2.24{\times}10^7{\pm}3.90{\times}10^7\;CFU/g$ and $5.90{\times}10^5{\pm}2.25{\times}10^6\;CFU/g$, respectively. The kochujang collected from various regions showed quite strong liquefying and saccharogenic amylase and protease at different level by samples.

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A Study on the Bibliographic Characteristics of Most-cited Articles - Focusing on the Domestic Social Science Field - (피인용 최상위 학술논문의 서지적 특성에 관한 연구 - 국내 사회과학 분야를 대상으로 -)

  • Lee, Soo-Sang
    • Journal of Korean Library and Information Science Society
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    • v.49 no.4
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    • pp.53-71
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    • 2018
  • The purpose of this study was to identify bibliographic characteristics of the top level articles among KCI indexed academic articles. Unlike the existing analysis on citation and most-cited articles, this study analyzed their bibliographic characteristics. The 3,453 articles were collected from 17 social science fields in KCI. The characteristics of authors, institutions, publications, journals, and citations were analyzed. According to the result of the analysis, there was a difference in the ratio of single authors to co-authors and in the number of co-authors. The authors were mostly single authors or two co-authors. In general, the percentage of authors of one article was high. Second, the types of institutions were mostly universities, and there were differences in the number of institutions with authors in each field. And the frequency of the authors' affiliation appeared uneven. There were a number of excellent research institutes and authors depending on the field. Third, the average of years to take to become the top articles for publication was 11 years in 2018. Fourth, most of the published journals were KCI registered journals, and the influence of the journals was not a factor. Fifth, there were differences in the number of cited counts between fields. This study is meaningful to grasp the influencing factors on the bibliographic characteristics of the most-cited articles for the social science field.