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Supplement of Conjugated Linoleic Acid Increases Neutrophil Phagocytosis in Pigs  

Sang-chul, Han (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine)
Ji-houn, Kang (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine)
Sung-mok, Son (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine)
Chung-soo, Chung (Department of Animal Science, Chungbuk National University)
Chul-young, Lee (Department of International Livestock Industry, Chinju National University)
Mhan-pyo, Yang (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine)
Publication Information
Journal of Veterinary Clinics / v.20, no.4, 2003 , pp. 437-442 More about this Journal
Abstract
To examine the in vivo immunostimulating effect of conjugated linoleic acid (CLA) in pigs, the change of peripheral blood cells and the phagocytic response of phagocytes were evaluated. Spayed male pigs, 80 kg of average body weight, fed a diet containing either 0.5% 10t-12c CLA or 0.5% CLA mixture (mostly 9c-11t CLA and 10t-12c CLA) for 4 weeks. The change of blood cell values (PCV, WBC, differential count of WBC) and the phagocytic activities of phagocytes were evaluated on week 0, 2, 4, and 5, respectively. There were no change in the PCV values regardless of CLA supplement. The number of WBC, especially neutrophils, in pigs fed a diet with CLA was significantly increased (p<0.05 to 0.01) when compared with control pigs fed a diet without CLA. The phagocytosis of peripheral blood mononuclear cell (MNC) and peripheral blood polymorphonuclear cells (PMN) were analyzed by a flow cytometry system. There was no change in the phagocytic activity of MNC and monocyte-rich cells regardless of CLA supplement. However, the phagocytic activity of PMN composed by approximately 95% neutrophils was remarkably increased (p < 0.05 to 0.01) on week 2, 4, and 5 as compared wth control pigs. These results suggested that supplement of CLA into pigs induces the increase of neutrophil number and the enhancement of neutrophil phagocytosis.
Keywords
conjugated linoleic acid (CLA); neutrophil; phagocytosis; pig;
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