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A Study on Seed Germination, Seedling Growth and Absorption of Inorganic Nutrients of Deutzia crenata Seedlings Treated with Poultry Manure (계분 처리에 의한 빈도리나무(Deutzia crenata) 종자발아·유묘생육 및 무기물 흡수에 관한 연구)

  • Lee, Chang-Heon;Kim, Mi-Ja;Jin, Jae-Jun;Kang, Hag-Mo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.6
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    • pp.15-25
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    • 2018
  • This study was conducted to obtain proper amount of solid poultry manure in the beginning phase of Deutzia crenata growth. Seedling growth increment, dry weight, inorganic matter uptake and chemical changes of soil according to the concentration of solid poultry manure fertilization. 1. When treated with solid poultry manure, seed germination rate was highest on the control. However, germination rates tended to decrease when treated with at high concentrations. 2. The growth of seedlings treated with poultry manure was always higher than that in control. At the 1.0% of poultry manure treatment, the growth rate and dry weight of the seedlings was highest. 3. The amount of inorganic nutrients absorbed by the seedling was generally high with the 1.0% treatment, declined sharply with the 2.0% treatment. 4. For the planting soil of Deutzia crenata, the higher the concentration of poultry manure, the lower the soil pH. However, nitrogen, available P, K, Na and Mg contents in the soil have increased with higher concentrations.

Determination of Thermal Radiation Emissivity and Absorptivity of Thermal Screens for Greenhouse (온실 스크린의 장파복사 방사율 및 흡수율 결정)

  • Rafiq, Adeel;Na, Wook Ho;Rasheed, Adnan;Kim, Hyeon Tae;Lee, Hyun Woo
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.311-321
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    • 2019
  • Greenhouse farmers often use thermal screens to reduce greenhouse heating expenses during the winter, and for shade during hot, sunny days in the summer, as it is an inexpensive solution to temperature control relative to other available options. However, accurate measurements of their emitted and absorbed radiations are important for the selection of suitable screens that offer maximum performance. Material's ability to save energy is highly dependent on these properties. Limited studies have investigated the measurement of these properties under natural conditions, but they are only applicable to materials having partial porosities. In this work, we describe a new radiation balance method for determining emissive power and absorptive capacity, as well as reflectivity, transmissivity and emissivity of materials having complete and partial transparency by using pyrgeometer and net radiometer. In this study, four materials with zero porosity, were tested. The emissivity value of PE, LD-13, LD-15 and PH-20 was $0.439{\pm}0.020$, $0.460{\pm}0.010$, $0.454{\pm}0.004$, and $0.499{\pm}0.006$, respectively. All tested samples showed high emitted radiation as compared to absorbed radiation.

Recent Academic Publishing Trends through Bibliometric Analysis of COVID-19 Articles: Focused on Medicine and Life Science (코로나19 연구논문의 계량서지학적 분석을 통한 최근 학술출판 동향 - 의학과 생명과학 분야를 중심으로 -)

  • Shin, Eun-Ja
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.32 no.1
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    • pp.115-132
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    • 2021
  • This study collected data on COVID-19 research papers published in international journals by Korean authors from WoS. Bibliographical analysis was performed on subject categories, institutions, funder distribution and so on. In addition, open access and journal review speed were also analyzed, which play an important role in facilitating academic publishing and distribution. The results showed that COVID-19-related papers published in international journals by Korean authors in 2020 included more papers on some specific fields, such as medicine, biology, and multidisciplinary. These researchers have published lots of papers not only in foreign journals but also in domestic English journals. 94% of papers were open access, and gold open access, which is available immediately after publication, was about 70% of the total. The COVID-19 orthopedic papers produced by Korean researchers were collected from PubMed and analyzed, and the average of review days was about 24 days. The analysis, including open access and review speed, showed that there has been an atmosphere of cooperation in the academic publishing ecosystem after the COVID-19 crisis. It would be desirable to continue this cooperation and address chronic problems in academic publishing system, such as promoting the publication of gold open access and reviewing efficiency.

Distributed Edge Computing for DNA-Based Intelligent Services and Applications: A Review (딥러닝을 사용하는 IoT빅데이터 인프라에 필요한 DNA 기술을 위한 분산 엣지 컴퓨팅기술 리뷰)

  • Alemayehu, Temesgen Seyoum;Cho, We-Duke
    • KIPS Transactions on Computer and Communication Systems
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    • v.9 no.12
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    • pp.291-306
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    • 2020
  • Nowadays, Data-Network-AI (DNA)-based intelligent services and applications have become a reality to provide a new dimension of services that improve the quality of life and productivity of businesses. Artificial intelligence (AI) can enhance the value of IoT data (data collected by IoT devices). The internet of things (IoT) promotes the learning and intelligence capability of AI. To extract insights from massive volume IoT data in real-time using deep learning, processing capability needs to happen in the IoT end devices where data is generated. However, deep learning requires a significant number of computational resources that may not be available at the IoT end devices. Such problems have been addressed by transporting bulks of data from the IoT end devices to the cloud datacenters for processing. But transferring IoT big data to the cloud incurs prohibitively high transmission delay and privacy issues which are a major concern. Edge computing, where distributed computing nodes are placed close to the IoT end devices, is a viable solution to meet the high computation and low-latency requirements and to preserve the privacy of users. This paper provides a comprehensive review of the current state of leveraging deep learning within edge computing to unleash the potential of IoT big data generated from IoT end devices. We believe that the revision will have a contribution to the development of DNA-based intelligent services and applications. It describes the different distributed training and inference architectures of deep learning models across multiple nodes of the edge computing platform. It also provides the different privacy-preserving approaches of deep learning on the edge computing environment and the various application domains where deep learning on the network edge can be useful. Finally, it discusses open issues and challenges leveraging deep learning within edge computing.

The status of food allergy and parental burden of preschoolers in Jeju area (제주지역 미취학 아동의 식품알레르기 현황 및 이에 따른 부모의 양육 부담)

  • Oh, Jeong Eun;Kim, Eunyoung;Lee, Yunkyoung
    • Journal of Nutrition and Health
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    • v.54 no.6
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    • pp.664-678
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    • 2021
  • Purpose: Food allergies in children are known to impact the quality of life for growing children as well as their parents. This study investigates the status of food allergy and its management among preschoolers, and evaluates the effect on parental burden. Methods: In May 2019, five kindergartens and daycare centers in Jeju area were randomly selected, and parents (n = 638) who had preschoolers younger than 6-year-old were surveyed using a questionnaire. A total of 387 participants were included in the final data analysis. Results: The percentage of children (aged 3 to 6 years old) who experienced food allergies was 16.8%; 44.6% had been diagnosed as having a food allergy by doctors. Food allergies were first experienced in more than two-thirds of the subjects when they were less than a year old. Egg and milk were the most frequent allergenic foods with some skin related symptoms. A mere 15.4% children suffering from food allergies were offered substitutes for their allergenic foods. Lastly, as compared to other parents, the parents of children who experienced food allergies had not only higher nutritional knowledge, but also heavier parental burden including emotional distress. Regardless of their nutritional knowledge, parents of children with food allergy expressed their willingness to attend nutrition education classes, if available in future. Conclusion: For prevention of food allergies and proper nourishment of children with food allergies, there is a necessity to provide customized-nutritional education for the affected parents to help alleviate their parental burden, especially for reducing emotional distress.

Plasma metabolites associated with physiological and biochemical indexes indicate the effect of caging stress on mallard ducks (Anas platyrhynchos)

  • Zheng, Chao;Wu, Yan;Liang, Zhen Hua;Pi, Jin Song;Cheng, Shi Bin;Wei, Wen Zhuo;Liu, Jing Bo;Lu, Li Zhi;Zhang, Hao
    • Animal Bioscience
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    • v.35 no.2
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    • pp.224-235
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    • 2022
  • Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks. Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database. Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted.

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

  • Wang, Laiyou;Guo, Shuxian;Zeng, Bo;Wang, Shanshan;Chen, Yan;Cheng, Shuang;Liu, Bingbing;Wang, Chunyan;Wang, Yu;Meng, Qingshan
    • Mycobiology
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    • v.50 no.1
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    • pp.66-78
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    • 2022
  • The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

A Study on Utilization Method of the Metaverse in Digital Heritage (디지털 문화유산의 메타버스 활용방안 연구)

  • Seol, Yeonsu;Joo, Chungmin;Yoo, Jongwon
    • Smart Media Journal
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    • v.10 no.4
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    • pp.111-120
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    • 2021
  • This study looked at how the digital cultural heritage, which is being actively built by the government and public institutions, can be utilized in the metaverse service. Through an expert interview, we investigated how the digital cultural heritage of Bulguksa can be serviced with the four elements of the metaverse: augmented reality, mirror world, lifelogging, and virtual world. As a result of the study, it was confirmed that when digital cultural heritage is used in a virtual space called metaverse, interactive and realistic services can be provided to users. In addition, it was found that users can exhibit, experience, and educate online parts that are not available in real life in the same situation as if they were in the field. Through this, it was confirmed that if digital cultural heritage is designed as a metaverse service, it can provide functions and services of a different level than before. In particular, the result was obtained that it is possible to provide services considering the characteristics of each of the four elements of the metaverse. First, it is possible to obtain the convenience of augmenting the senses by using augmented reality, and secondly, it is possible to obtain the scalability and efficiency of reproducing the real space by using it as a mirror world element. Third, by using the lifelogging element, communication can be strengthened through the user's record and connection, and fourthly, through the virtual world element, roles and activities in the virtual world can be given to the user. Therefore, if digital cultural heritage is developed as a metaverse service in consideration of these factors, a more active and open experience environment can be provided to users.

Establishment of intestinal organoids from small intestine of growing cattle (12 months old)

  • Kang Won, Park;Hyeon, Yang;Min Gook, Lee;Sun A, Ock;Hayeon, Wi;Poongyeon, Lee;In-Sul, Hwang;Jae Gyu, Yoo;Choon-Keun, Park;Bo Ram, Lee
    • Journal of Animal Science and Technology
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    • v.64 no.6
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    • pp.1105-1116
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    • 2022
  • Recently, we reported the robust in vitro three-dimensional (3D) expansion of intestinal organoids derived from adult bovine (> 24 months) samples. The present study aimed to establish an in vitro 3D system for the cultivation of intestinal organoids derived from growing cattle (12 months old) for practical use as a potential alternative to in vivo systems for various purposes. However, very few studies on the functional characterization and 3D expansion of adult stem cells from livestock species compared to those from other species are available. In this study, intestinal crypts, including intestinal stem cells, from the small intestines (ileum and jejunum) of growing cattle were isolated and long-term 3D cultures were successfully established using a scaffold-based method. Furthermore, we generated an apical-out intestinal organoid derived from growing cattle. Interestingly, intestinal organoids derived from the ileum, but not the jejunum, could be expanded without losing the ability to recapitulate crypts, and these organoids specifically expressed several specific markers of intestinal stem cells and the intestinal epithelium. Furthermore, these organoids exhibited key functionality with regard to high permeability for compounds up to 4 kDa in size (e.g., fluorescein isothiocyanate [FITC]-dextran), indicating that apical-out intestinal organoids are better than other models. Collectively, these results indicate the establishment of growing cattle-derived intestinal organoids and subsequent generation of apical-out intestinal organoids. These organoids may be valuable tools and potential alternatives to in vivo systems for examining host-pathogen interactions involving epithelial cells, such as enteric virus infection and nutrient absorption, and may be used for various purposes.

Alternative Immunossays

  • Barnard, G.J.R.;Kim, J.B.;Collins, W.P.
    • Korean Journal of Animal Reproduction
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    • v.9 no.2
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    • pp.133-139
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    • 1985
  • An immunoassay may be defined as an analytical procedure involving the competitive reaction between a limiting concentration of specific antibody and two populations of antigen, one of which is labelled or immobillized. The advent of immunoassay has revolutionised our knowledge of reproductive physiology and the practice of veterinary and clinical medicine. Radioimmunoassay (RIA) was the first of these methods to be developed, which meausred the analyte with good sensitivity, accuracy and precision (1,2). The essential components of RIA are:-(i) a limited concentration of antibodies, (ii) a reference preparation, and (iii) an antigen labelled with a radioisotope (usually tritium or iodine-125). Most procedures invelove isolating the antibody-bound fraction and measuring the amount of labelled antigen. Good facilities are available for scintilltion counting, data reduction nd statistical analysis. RIA is undergoing refinement through:-(i) the introduction of new techniques to separate the antibody-bound and free fractions which minimize the misclassification of labelled antigen into these compartments, and the amount of non-specfic binding. (3), (ii) the development of non-extration for the measurement of haptens (4), (iii) the determination of a, pp.rent free (i.e. non-protein bound) analytes (5), and (iv) the use of monoclonal antibodies(6). In 1968, Miles and Hales introduced in important new type of immunoassay which they termed immunora-diometric assay (IRMA) based on t도 use of isotopically labelled specific antibodies(7) in a move from limited to excess reagent systems. The concept of two-site IRMAs (with a capture antibody on a solid-phase, and a second labelled antibody to a different antigenic determinant of the analyte) has enabled the development of more sensitive and less-time consuming methods for the measurement of protein hormones ovar wide concentration of analyte (8). The increasing use of isotopic methos for diverse a, pp.ications has exposed several problems. For example, the radioactive half-life and radiolysis of the labelled reagent limits assay sensitivity and imposes a time limit on the usefulness of a kit. In addition, the potential health hazards associated with the use and disposal of radioactive cmpounds and the solvents and photofluors necessary for liquid scientillation counting are incompatable with the development of extra-laboratory tests. To date, the most practical alternative labels to radioisotopes, for the measurement of analytes in a concentration > 1 ng/ml, are erythrocytes, polystyrene particiles, gold sols, dyes and enzymes or cofactors with a visual or colorimetric end-point(9). Increased sensitivity to<1 pg/ml may be obtained with fluorescent and chemiluminescent labels, or enzymes with a fluorometric, chemiluminometric or bioluminometric end-point. The sensitivity of any immunoassay or immunometric assay depends on the affinity of the antibody-antigen reaction, the specific activity of the label, the precision with which the reagents are manipulated and the nonspecific background signal (10). The sensitivity of a limited reagent system for the measurement of haptens or proteins is mainly dependent upon the affinity of the antibodies and the smalleest amount of reagent that may be manipulated. Consequently, it is difficult in practice to improve on the sensitivity obtained with iodine-125 as the label. Conversely, with excess reagent systems for the measurement of proteins it is theoretically possible to increase assay sensitivity at least 1000 fold with alternative luminescent labels. To date, a 10-fold improvement has been achieved, and attempts are being made to reduce the influence of other variables on the specific signal from the immunoreaction.

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