• Title/Summary/Keyword: availability growth

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Study of the Function of β-D-fructofuranosidase by Plant Histological Method (식물 조직학적 방법에 의한 β-D-fructofuranosidase의 기능 연구)

  • Donggiun Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.587-592
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    • 2023
  • Plant growth is regulated by a variety of factors, including organic matter availability. Organic nutrients are carbohydrate molecules from photosynthetic products produced by tissues associated with carbon and energy fixation called "sources". These compounds flow through plant vascular bundles into non-photosynthetic or growing tissues called "sinks". Among these possible compounds, the disaccharide fructosyl glucose, sucrose, is the most representative. During the transport of sucrose, the pathway from the source to the sinks can include hydrolysis of sucrose into glucose and fructose derivatives or direct transfer of sucrose. Among the enzymes involved in this, β-D-fructofuranosidase is the most important. Soluble neutral β-D-fructofuranosidase, one of several isoenzymes, is located in intracellular protoplasts and helps plant cells metabolize sucrose to produce energy. In order to track the activity of this enzyme during the course of plant growth, histological methods were used for the most effective immunolocalization. As a result, the activity was higher in the phloem and epidermis than in the mesophyll tissue in the leaf. In the growing stem, activity was high in the phloem, epidermis, and cortex. The activity of the root, which is a sink tissue, was high in all parts, but especially the highest in the root tip part. It is thought that this is because it helps unloading of sucrose in sink tissues that require sucrose degradation and plays a role in hydrolysising sucrose.

Application Effect of Chlorella Fusca CHK0059 as a Biofertilizer for Strawberry Cultivation (딸기재배를 위한 생물비료로 Chlorella fusca CHK0059의 시비효과)

  • Young-Nam Kim;Jun Hyeok Choi;Song Yeob Kim;Hyeonji Choe;Yerim Shin;Young-Eun Yoon;Keum-Ah Lee;Min-Jeong Kim;Yong Bok Lee
    • Korean Journal of Environmental Agriculture
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    • v.41 no.4
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    • pp.282-287
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    • 2022
  • BACKGROUND: Green algae (Chlorella spp.) has been widely used as a biofertilizer to improve the crop yield and quality. However, available information is not enough to verify the mechanism of green algae's beneficial impact on strawberry. This study was conducted to investigate the effect of Chlorella fusca CHK005 application on the growth and nutrient status of strawberry plant and fruit characteristics. METHODS AND RESULTS: A total of 800 seedlings of strawberry variety 'Kuemsil' were planted. Once a week, C. fusca culture solution (1.0 × 107 cells mL-1) was applied into soil via irrigation in four different concentrations: no application (control), 1/1000 times (× 0.5), 1/500 times (× 1), and 1/250 times (× 2). Result showed that growth of strawberry plant was enhanced by Chlorella application and the highest impact on fresh weight (FW) and chlorophyll content of the plants were observed in × 2 treatment, followed by × 1, × 0.5, and control treatments. The phosphorus (P) concentration in the plant was significantly higher in × 1 and × 2 treatments compared to control. In case of fruit quality, sugar content (°Brix), hardness, and FW were lowest in control, but these values increased as application levels of Chlorella were higher. Also, P and K contents in the fruits increased with increasing the application levels and significant correlation between P content and oBrix in the fruits was found. CONCLUSION(S): Overall, Chlorella application seemed to improve plant growth and fruit quality by increasing the utilization efficiency of P and K in strawberries.

Shading Treatment-Induced Changes in Physiological Characteristics of Thermopsis lupinoides (L.) Link (차광처리에 따른 갯활량나물의 생리 특성)

  • Seungju Jo;Dong-Hak Kim;Jung-Won Yoon;Eun Ju Cheong
    • Journal of Korean Society of Forest Science
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    • v.113 no.2
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    • pp.198-209
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    • 2024
  • This study aimed to investigate the impact of light intensity, manipulated through different shading levels, on the growth and physiological responses of Thermopsis lupinoides. To assess the effects of shading treatments, we examined leaf mass per area, chlorophyll content, chlorophyll fluorescence response, and photosynthetic characteristics. T. lupinoidesexhibited adaptive responses under low light conditions (50% shading), showing increased leaf area and decreased leaf mass per area as shading levels increased. These changes indicate morpho-physiological adaptations to reduced light availability. At 50% shading, the physiological and ecological responses were favorable, with optimal photosynthetic functions including chlorophyll content, photosynthesis saturation point, photosynthetic rate, carbon fixation efficiency, stomatal conductance, transpiration rate, and water use efficiency. However, at 95% shading, the essential light conditions for growth were not met, significantly impairing photosynthetic functions. Consequently, 50% shading was determined to be the most optimal condition for T. lupinoides growth. These findings provide valuable insights for effective ex-situconservation practices and site selection for T. lupinoides, serving as foundational data for habitat restoration efforts.

Enhancement of Plant Growth and Drying Stress Tolerance by Bacillus velezensis YP2 Colonizing Kale Root Endosphere (Bacillus velezensis YP2 균주의 근권 정착에 의한 케일의 생육 촉진 및 건조 스트레스 완화 효과)

  • Kim, Da-Yeon;Han, Ji-Hee;Kim, Jung-Jun;Lee, Sang-Yeob
    • Korean Journal of Organic Agriculture
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    • v.26 no.2
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    • pp.217-232
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    • 2018
  • Drought is a major obstacle to high agricultural productivity, worldwide. In drought, it is usually presented by the simultaneous action of high temperature and drying. Also there are negative effects of plant growth under drying conditions. In this study, the effect of Bacillus velezensis YP2 on plant growth-promotion and soil drying stress tolerance of kale plants, Brassica oleracea var. alboglabra Bailey, were investigated under two different conditions; greenhouse and field environments. Root colonization ability of B. velezensis YP2 was also analysed by using plating culture method. As a result of the greenhouse test, the YP2 strain significantly promoted the growth of kale seedlings in increasement of 26.7% of plant height and 142.2% of shoot fresh weight compared to control. B. velezensis YP2 have the mitigation effect of drying injury of kale by decreasing of 39.4% compared to control. In the field test, B. velezensis YP2 strain was also found to be effective for plant growth-promotion and mitigation of drying stress injury on kale plants. Especially, relative water contents (RWC; %) were higher in B. velezensis YP2 treated kales than in control at 7, 10, 14 day after non-watering. The root colonization ability of YP2 strain was continued at least for 21 days after soil drenching treatment of B. velezensis YP2. Our result suggested that enhancement of plant growth and drying injury reduction of kale plants were involved in kale root colonization by B. velezensis YP2, which might be contributed to increasing water availability of plants. Consequentially, the use of B. velezensis YP2 might be a beneficial influence for improving productivity of kale plants under drying stress conditions.

Effects of Nursery Environmental Factors on the Growth of Pacific Oyste, Crassostrea gigas (양식어장 환경요인이 참굴 (Crassostrea gigas)의 성장에 미치는 영향)

  • 배평암;한창희
    • Journal of Aquaculture
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    • v.11 no.3
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    • pp.391-400
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    • 1998
  • This study was performed to investigate the effects of environmental parameters on the growth of Pacific oyster, Crassostrea gigas at old suspended culturing grounds of Korsung Bay and newly deveoped bottom, culturing grounds of Haenam region located in southern coast of Korea. The Kosung Bay was semiclosed and water exchange occurred through the small mouth and the current speed was very slow as the value of below 0.5kn. Haenam was opened and littoral zone. Current speed of the surroundings of this Bay about to 5.0knand transparency was low with the mean depth of 1.2m by the resuspension of the sediments. Water temperature and salinity regimes were suitable for oysters at those sites, however the mean value of those parameters and seasonal fluctuations was high at Kosung. The mean concentration of dissolved oxygen in survey area was above $5.0mg/\ell$. In Kosung, however, it was decreasing in June and showed the minimum with the concentration of $4.0mg/\ell$ in August at the bottom layer and recovered in September. Eutrophication parameters such as COD were 1.44 mg/l at this location. This mean value was higher than that of the Haenam Bay's $0.96mg/\ell$. The men value of chlorophyll-$\alpha$ at Haenam was two times higher than the other sites. Growth of shell height and meat weight of oysters at Kosung Bay were higher and the fatness was lower compared to thost of Haenam. As a result, absolute growth of oysters was high at Kosung and allometric growth, such as fatness, was good at Haenam. Low water temperature and frequent exposure of oysters to the air during ebb tide may retard the shell growth. On the other hand, relatively high food availability was profitable for the fatness at haenam site.

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Growth-promoting Effect of New Iron-chelating Fertilizer on Lettuce (산세수와 게껍질을 이용한 신기능성 철분 비료의 상추 생육 촉진 효과)

  • Hwang, Ji Young;Jun, Sang Eun;Park, Nam-Jo;Oh, Ju Sung;Lee, Yong Jik;Sohn, Eun Ju;Kim, Gyung-Tae
    • Journal of Life Science
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    • v.27 no.4
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    • pp.390-397
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    • 2017
  • Iron (Fe) is an important micronutrient for the health and growth of plants. Iron is usually provided by fertilizers, and iron-chelate fertilizers are well absorbed by plants. This study presents the plant growth-promoting effects of a new functional iron fertilizer, Fe-chelating crab shell powder (FCSP), which is generated from the chelation of Fe ions with crab shell powder. Iron chelate was derived from spent pickling liquor, which is rich in reductive iron, iron(II) oxide. To analyze the effects of FCSP on plant growth, we treated lettuce with several concentrations of FCSP in both lab- and field-scale experiments. In the lab-scale test, the treatment of 50 ppm of FCSP highly promoted growth and resulted in increases in the size, weight, number and chlorophylls content of leaves of plants compared to the treatment of crab shell powder. Fifty ppm of FCSP also increased the size and weight of leaves up to 2 times compared to the application of chemical fertilizer and/or compost in field conditions. In addition, the FCSP treatment resulted in the highest ion uptake of Fe in lettuce leaves. Moreover, FCSP led to increases in the amounts of Fe, Ca, available phosphorus and organic matter in treated soil, indicating that soil quality was improved. Taken together, our results demonstrate that FCSP promotes lettuce growth via enhancement of Fe availability and improves soil quality. Therefore, FCSP can be utilized as a new functional iron fertilizer.

Phosphorus availability of water-logged soil (논토양(土壤)의 인산유효도(燐酸有効度)에 관(關)한 연구(硏究))

  • Chung, Yee-Keun;Hong, Chong-Woon
    • Korean Journal of Soil Science and Fertilizer
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    • v.10 no.1
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    • pp.55-60
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    • 1977
  • In order to study the availability of soil P and applied P to rice plant under water-logged system, a pot experiment with five soils having different levels of available P (24, 64, 100, 144 and 231 ppm) under four levels of applied P (0, 30, 60 and 90 ppm) was conducted. The availability of P was measured in terms of plant performance and the behaviors of P in soils were studied through the fractionation of various forms of P and by measuring the adsorption and desorption characteristics. The results are summarized us following. 1. The rice plant responded to applied P in the soils containing less than 144 ppm of available P as measured by Lancaster's method in terms of number of tillers in early growth stages. However, when the response of rice plant to applied P was evaluated in terms of grain yield there was no response even in the soil containing 24 ppm of available P. 2. Applied P was fixed as Al-P at the early stages and converted into Fe-P at later stages. 3. The P adsorption maxima of soils measured by Langmuir's isotherm ranged from 70 to 100mg/100g. No relationships between the level of available P and P adsorption maxima were observed. 4. There was a trend that the higher the level of available P, the higher the release of water soluble p. 5. The reduction of soil increased the level of available P by the factor of 1.8 times of air dried soils.

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Reset of Cultivation Zones of Green Manure Crops under Paddy Field in South Korea: Focused on Minimum Temperature and Drainage Class (국내 주요 녹비작물 논재배 적지 재설정: 1월 평균 최저기온 및 배수등급을 기준으로)

  • Kim, Min-Tae;Kim, Kwang Seop;Park, Ki Do;Ryu, Jin-Hee;Choi, Jong-Seo;Ku, Ja-Hwan;Kim, Suk-Jin;Lee, Choon-Woo;Lee, Kyungdo;Kang, Hang-Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.1
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    • pp.91-96
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    • 2015
  • Stable production of green manure crop is one of the critical factor for successful implementation of environmental-friendly agriculture in South Korea. Most green manure crops including hairy vetch cultivated in South Korea must be passed cold winter season. This study was conducted to establishment of cultivation zones on green manure crops under paddy field in South Korea using on minimum temperature and drainage class. In order to build the spatial distribution map, hairy vetch, triticale, barley, rye, and clover as green manure crop were selected and mean of daily minimum temperature and drainage class in paddy field were considered as limiting factor for stable growth of green manure crop. And cultivation zone on green manure crops was divided into two categories; 'Availability area' and 'Safety area'. Low limit temperature on green manure crops in this study was the following. Temperature on 'Availability area' was $-10^{\circ}C$, $-8^{\circ}C$, $-6^{\circ}C$, and $-20^{\circ}C$ for hairy vetch (including triticale), barley, clover, and rye respectively. And temperature on 'Safety area' was lower $-2^{\circ}C$ compared to that of 'Availability area' exception rye which was the same degree. Rye was expected to safely cultivated the almost whole paddy field (99.71%) of South Korea. 'Safety area' among the whole paddy field for hairy vetch, barley, and clover were 86.58, 53.06, and 15.76% respectively. The spatial distribution map of green manure crops reestablished in this study could be used the policy and agricultural management for environmental-friendly agriculture using green manure crops in South Korea.

Utilization of Liquid Pig Manure as a Substitute for Chemical Fertilizer in Double Cropping system of Rice Followed by Onion (벼·양파 작부체계에서 화학비료 절감을 위한 돈분뇨액비의 활용)

  • Lee, Jong-Tae;Lee, Chan-Jung;Kim, Hee-Dae
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.3
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    • pp.149-155
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    • 2004
  • This study was conducted to evaluate the availability of liquid pig manure (LPM) as a substitute for chemical fertilizer (CF) in double cropping system of rice followed by onion. The LPM applied for rice in silty clay loam soil cultivated in the double cropping system with onion contained $3.8g\;N\;kg^{-1}$, $1.8g\;P_2O_5\;kg^{-1}$ and $2.7g\;K_2O\;kg^{-1}$. The LPM applied for onion after rice contained $4.9g\;N\;kg^{-1}$, $1.4g\;P_2O_5\;kg^{-1}$ and $2.1g\;K_2O\;kg^{-1}$. Soil pH increased after rice culture irrespective of treatments. The rice growth and grain yield among CF and LPM applications were not significantly different. The application of LPM without top dressing of CF delayed onion growth at mid and late stage. But when the LPM was applied as basal fertilizer and CF was added for top dressing, onion growth was maintained until late growth stage. The nutrient uptake of LPM and top-dressing by CF (rice)/LPM and top-dressing by CF (onion) were similar to CF (rice)/CF (onion). The highest yield of onion bulb was 58.5 Mg ha-1 at CF (rice)/LPM and top-dressing by CF (onion) treatment, but showed no significant difference with other treatments except CF (rice)/LPM only (onion) and no fertilization (rice)/no fertilization (onion) treatments. In conclusion, in double cropping system of rice followed by onion, rice was capable of being grown by only liquid pig manure but additive chemical fertilizer was needed for optimal onion growth.

Effect of Water Activity and Temperature on Growth, Germination, Sporulation, and Utilization of Carbon Source of Penicillium oxalicum (PENOX) as a Biocontrol Agent(BCA) for control of Clover(Trifolium repens L.) (토끼풀(Trifolium repens L.) 방제용 생물제제 Penicillium oxalicum (PENOX)의 발아, 생장, 포자생성 및 탄소원이용에 미치는 수분활성 및 온도의 영향)

  • Lee, Hyang-Burm;Kim, Chang-Jin
    • The Korean Journal of Pesticide Science
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    • v.4 no.3
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    • pp.68-74
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    • 2000
  • Penicillium oxalicum (PENOX) has shown the potential as a biocontrol agent(5CA) for control of a weed, clover(Trifolium repens L.) in grass plots. The bioherbicidal activity may be due to germinative and growth capacities and substrate availability of the agent over a range of environmental factors. The influences of different water activities($0.94{\sim}0.995\;a_w$) and temperatures($18{\sim}30^{\circ}C$) on mycelial growth, conidial germination, sporulation oil 2% MEA(malt extract agar) adjusted to different water activities with glycerol, and carbon source utilization using BIOLOG GN MicroPlate were determined in vitro. Decreases in $a_w$ on MEA caused a reduction in mycelial growth and conidial germination depending on temperature. The mycelial growth of PENOX was greatest at $30^{\circ}C/0.995\;a_w$. At some lowered water activity($0.97\;a_w$), the growth was similar between 25 and $30^{\circ}C$, and considerably decreased at lowered temperature($20^{\circ}C$). The germination rate was also greatest at $30^{\circ}C/0.995\;a_w$. Lag phase times for PENOX at $18^{\circ}C$ on MEA were >6hrs at tile whole $a_w$ level tested, and at 18 and $25^{\circ}C$ they were >18hrs and >12hrs at $0.94\;a_w$, respectively. However, its sporulation was some better at $0.97\;a_w$ than $0.995\;a_w$ or $0.94\;a_w$, and better at $20^{\circ}C$ than $30^{\circ}C$. In contrast, the number of carbon sources(niche size) utilized by PENOX varied with $a_w$ and temperature. Under some water stress condition($0.95\;a_w$), the agent utilized smaller number of carbon sources than $0.995\;a_w$ depending on temperature. The niche size at 0.995 and $0.95\;a_w$ were highest at $25^{\circ}C$, and showed 86 and 65, respectively. At $30^{\circ}C$, the niche size at 0.995 and $0.95\;a_w$ showed 84 and 50, respectively. There was no carbon source utilized by PENOX at $0.90\;a_w$ regardless of temperature. These information of tile fungal ecophysiology will be useful for the effective development of BCA.

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