• 문화기술의 융합
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• 제10권1호
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• pp.579-584
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• 2024

현재 우리나라는 지하철 선로를 인접하여 대규모, 대심도 굴착시공이 이루어지고 있다. 인접굴착공사 시 흙막이 구조물 및 지하구조물의 안전성 확보가 매우 중요하므로 자동화계측 시스템을 도입하여 지하철에 대한 안전성을 관리하고 있다. 인접굴착공사 시 지하철 궤도 변형은 열차주행안정성에 영향을 미칠 수 있으며, 이는 열차 탈선사고와 연결될 수 있는 인자이다. 그러나 현재 자동화계측 시스템을 이용한 지하철 궤도 안전성평가는 측정된 데이터의 최댓값에만 의존하여, 이상거동을 과소, 과대평가할 수 있는 기법이다. 따라서 자동화계측 시스템 결과의 활용도를 개선시킬 수 있는 방법이 필요한 실정이다. 이에 본 연구에서는 방대한 양의 지하철 궤도 변형 측정결과를 정량적으로 평가할 수 있는 기법인 가우시안 확률밀도함수 분석기법을 이용하여 분석하였다. 방대한 양의 데이터를 확률통계 분석기법을 이용하여 인접굴착공사에 따른 지하철 궤도 변형에 대한 안전성평가를 수행하였다.

• Tuberculosis and Respiratory Diseases
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• 제61권1호
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• pp.54-59
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• 2006

연구 배경 : 결핵의 대부분을 차지하는 폐결핵 진단에 객담, 기관지 세척액, 흉수액을 이용한 TB-PCR 검사의 유용성에 대해서는 여러 연구가 있었으나 폐 이외의 결핵 진단을 위한 신선 생검 조직 검체에서의 TB-PCR법 연구는 아직 미흡하다. 이에 저자들은 자동분석법인 COBAS AMPLICOR MTB PCR assay (Roche Molecular System)를 이용하여 신선 생검 조직 검체로 TB-PCR법의 유용성을 알아보았다. 방법 및 대상 : 2004년 10월부터 2005년 12월까지 가톨릭대학교 대전성모병원 병리과와 진단검사의학과에 결핵의진 하에 조직검사와 신선조직을 이용한 항산균 도말검사, 배양검사, TB-PCR 검사가 공히 의뢰된 환자 42예를 대상으로 하였다. 결 과 : 신선 생검 조직 42예를 대상으로 실시한 결과, 임상소견에서 결핵으로 진단된 경우는 18예 이었으며, 그 중 림프절 12예와 폐 조직 2예, 충수 조직 1예, 총 16예(88.9%)에서 PCR 양성을 보였고, 민감도와 특이도, 양성예측도, 음성 예측도는 88.9%, 100%, 100%와 92.3%로 나타났다. 조직학적으로 육아종 소견과 건락성 괴사의 소견 보인 18예(100%)는 모두 결핵으로 진단되었고 그 중 16예(88.9%)에서 PCR 양성을 보였다. 결 론 : 신선 생검 조직 검체를 이용한 TB-PCR 자동분석기의 결과는 임상소견 및 현미경적 소견과 비교 분석 시 민감도와 특이도가 높은 유용한 검사라고 생각되었다.

• Agricultural and Biosystems Engineering
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• 제1권1호
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• pp.59-62
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• 2000

After ten years of implementing the agricultural automation program in Taiwan, some positive effects and satisfactory results have been recognized by both the agricultural industry and local administrative bureaux. The automation of agriculture is a response to sophisticated demands for production and quality in countries with high labor costs. The development of sensor systems, control systems, precision agriculture systems, and engineering for plant culture systems will determine the degree of automation used for crop production in the 21st century. The engineering system will capitalize upon expertise from physiologists, pathologists, systems analysts, agronomists, horticulturists, computer programmers, economists, crop producers and managers in order to efficiently implement automated crop production.

• International Journal of Advanced Culture Technology
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• 제10권3호
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• pp.393-396
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• 2022

Recently, rapid climate change has had a significant impact on the ecosystem of honeybees. In addition, the problem of Vespa Hornets invasion of colonies has a fatal impact on the bee ecosystem, independent of climate change. Especially in late summer. This study relates to a method for preventing Vespa Hornets attack. In this study, we developed a Vespa Hornets sound detection device was developed by collecting and analyzing the sound of a Vespa Hornets and applying IoT technology. The developed device detects the sound of a Vespa Hornets when Vespa Hornets appears around the hive of the bees and sends a signal to automatically close the door of the beehive. The device that receives the signal drives the motor that controls the honeycomb door to close the beehive door. The Vespa Hornets sound detection device operates until no Vespa Hornets sound is detected. The system developed by us is expected to be installed in the beehives of actual beekeeping farms to dramatically reduce the damage caused by by Vespa Hornets.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1868-1874
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• 2007

We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns${\times}$six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handler is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins.

• Biomolecules & Therapeutics
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• 제22권4호
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• pp.355-362
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• 2014

We have developed a fully automated high throughput drug screening (HTDS) system based on the microfluidic cell culture array to perform combinational chemotherapy. This system has 64 individually addressable cell culture chambers where the sequential combinatorial concentrations of two different drugs can be generated by two microfluidic diffusive mixers. Each diffusive mixer has two integrated micropumps connected to the media and the drug reservoirs respectively for generating the desired combination without the need for any extra equipment to perfuse the solution such as syringe pumps. The cell array is periodically exposed to the drug combination with the programmed LabVIEW system during a couple of days without extra handling after seeding the cells into the microfluidic device and also, this device does not require the continuous generation of solutions compared to the previous systems. Therefore, the total amount of drug being consumed per experiment is less than a few hundred micro liters in each reservoir. The utility of this system is demonstrated through investigating the viability of the prostate cancer PC3 cell line with the combinational treatments of curcumin and tumor necrosis factor-alpha related apoptosis inducing ligand (TRAIL). Our results suggest that the system can be used for screening and optimizing drug combination with a small amount of reagent for combinatorial chemotherapy against cancer cells.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1259-1264
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• 2009

We assessed the capacity of two liquid-medium culture methods with automated incubation and reading systems (MB/BacT ALERT 3D System and BACTEC MGIT 960 System) and one solid-medium culture method ($L\ddot{o}wenstein$-Jensen) to detect mycobacteria in different types of clinical samples. Out of 1,770 cultured clinical samples (1,519 of respiratory origin and 251 of non respiratory origin), mycobacteria were isolated in 156 samples (135 M. tuberculosis complex, 8 M. chelonae, 6 M. kansasii, 4 M. fortuitum, 2 M. gordonae, and 1 M. marinum) by at least one of the methods used. The BACTEC MGIT 960 System proved to be the most sensitive method (86.5%), especially in the detection of M. tuberculosis complex (89.1%). However, $L\ddot{o}wenstein$-Jensen culture was the most sensitive (76.2%) to detect nontuberculous mycobacteria. The BACTEC MGIT 960 System showed the lowest mean detection time for mycobacterial growth (15.3 days), significantly shorter than the other two methods. Highest sensitivity (95.5%) and specificity (99.6%) values were obtained using the BACTEC MGIT 960 System with the $L\ddot{o}wenstein$-Jensen culture method, which was also the only combination capable of detecting 100% of the nontuberculous mycobacteria.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.307-312
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• 2004

식물조직배양용 바이오리액터 8개의 배양액 P보, DO 농도를 on-line으로 계측하고 pH 농도 및 주입공기 량을 제어할 수 있는 시스템을 개발하였다. 시스템 제어용 컴퓨터 프로그램은 나리구근의 성장단계에 맞는 주입공기량을 추종제어방식으로 제어하며, 바이오리액터 내부의 pH 변화를 감지하여 오염을 감시하는 오염경보기능이 포함되어있다. 성장단계에 따라 적절한 주입공기량의 선정을 위하여 시뮬레이션 한 결과 배양초기에는 300 cc/min, 20일 경과 후에는 400 cc/mim, 40일 경과 후에는 500 cc/min, 60일 경과 후에는 600 cc/min, 그리고 80일 경과 후부터는 700 cc/min의 공기를 주입할 경우 바이오리액터내 나리구근의 분포가 고르게 나타났으며, 이 결과를 바이오리액터 배양실험에 이용하였다 배양액의 pH 농도 제어 시스템은 배양 전 기간동안에 제어 목표 값 (5.5$\pm$0.5)로 제어할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• 한국안전학회지
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• 제39권3호
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• pp.68-74
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• 2024

Despite the growing importance of safe railroad operation with the recent implementation of the Severe Accident Punishment Act, industrial accidents related to the shunting of railway vehicles persist. Therefore, the government and Korea Railroad Corporation are collaborating on an automated connection and disconnection system that enables remote control of shunting trains from a central control room, utilizing surveillance cameras. By eliminating the need for on-site workers during freight car operations at stations, it aims to reduce safety accidents inherent in manual receiving processes. A qualifying government-issued railway vehicle driver's license is necessary to handle this newly developed automatic connection and disconnection system. As a result, this study aims to establish an appropriate driver qualification system.