• The Korean Journal of Ecology
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• v.22 no.1
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• pp.39-43
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• 1999

Serratia marcescens, an enterobacterium of gram-negative bacteria, is characterized by resistance of the admium. Cadmium sensitive PM strain did not grow in the medium at cadmium concentration of 50 ppm. PA strain was induced to accommodate to cadmium by cultivating the mother strain (PC strain) in the medium with 50 ppm cadmium. As compared with PC and PM strains, PA strain revealed the excellent growth in cadmium media and accumulated four to five times higher cadmium concentration in cell than other strains. PA strain accumulated 23% of cadmium in cells when cultured in medium treated with 100 ppm cadmium and this cadmium was more accumulated in cytosol fractions than membrane fractions. Analysis by TEM indicated that cadmium was concentrated as a form of granule in cytosol. In protein patterns of cell after the treatment of cadmium, two inducible proteins (28 KDa and 64 KDa) and one reducible protein (45 KDa) were detected by SDS-PAGE. By Atomic Absorption Spectrophotometer, the amounts of cadmium attached to inducible proteins of 28 KDa and 64 KDa were 318.28 ㎍ and 325.37 ㎍ per gram of protein, respectively. It is assumed that these inducible proteins play an important role in the mechanism of cadmium accumulation in cells. A plasmid of 23Kbp was found in S. marcescens. The ability of resistance to cadmium in plasmid was confirmed by curing experiments.

• Applied Microscopy
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• v.23 no.2
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• pp.53-77
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• 1993

In this study, an attempt was made to investigate the probable organelles participating in the secretion of biligrafin. The animals (ICR male mice, 25-30gm) were divided into normal control and 6 biligrafin injected groups to which 30% biligrafin (0.006ml/gm b.w.) were injected at 10, 20, 40, 80, 160 and 320 min prior to the sampling. The mice of each group were perfused through the heart with ice-cold 2.5% glutaraldehyde buffered with 0.1M Na-cacodylate (pH. 7.4) under the Na-pentobarbital (Nembtal 0.0015mg/gm b.w.) anesthesia and liver tissues were taken from each group. Some specimens were immersed 1 hr in the same solution used in the perfusion. After an overnight rinse in 0.1M Na-cacodylate buffer containing 10% DMSO and 7.6% sucrose, $75{\mu}m$ fronzen sections were made for cytochemical study. The sections were incubated in thiamin pyrophosphatase (TPPase) and inosine diphosphatase (ID Pase) media for 70 min at $37^{\circ}C$ respectively and acid phosphatase (AcPase) medium for 40 min at $37^{\circ}C$. They were postfixed in 1 % $OsO_4$ for 1 hr. The other specimens were immersed for 8 hrs in the fixative consisting of 2.5% glutaraldehyde and 3.0% paraformaldehyde buffered with Na-cacodylate (pH. 7.4). All of the osmificated specimens were processed for electron microscopy. In both normal and biligrafin injected groups, endoplasmic reticulum (ER), vacuoles, Golgi apparatus and lysosomes were seen in the vicinity of bile canaliculus. In the biligrafin injected groups, however, the Golgi apparatus appeared to be decreased and ER and vacuoles were dilated and increased. The rough endoplasmic reticulum (RER) having a few attached ribosomes appeared to be the round saccule, especially at 20 min after biligrafin injection. Smooth endoplasmic reticulum (SER) seemed to be formed by the detachment of ribosomes at the cisternal end of RER. The cistern of SER showed saccules which probably budded off to form the vacuole. The vacuoles were devoid of visible centents. This finding seemed to be in agreement with the biochemical property of the bile constituents. The fusion between the vacuoles and bile canaliculus were frequently seen in the groups injected with biligrafin. The lysosome did not show any changes in the biligrafin injected groups. Accumulation of some material and lipid droplets were seen at the 40 and 80 min after biligrafin injection, especially at the latter. At 160 and 320 min after biligrafin injections, however, they were decreased successively while the RER stack, free ribosomes and polysomes were increased. Although the reactive products of TPPase and IDPase were observed in the ER saccules and vesicles of the normal control and biligrafin injected groups, the fusion between the bile canaliculus and saccules or vesicles could easily be seen in the latter. The AcPase activity, however, was observed in the cistern at the maturing face of Golgi apparatus and lysosomes in both normal and biligrafin groups. The results suggest that the biligrafin is excreted via the vesicles, vacuoles or sacoules probably derived from the SER without the participation of Golgi apparatus and lysosomes, and the excess amount of material is stored as inclusions during the repairing of the organelles being overactive.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.4
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• pp.861-866
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• 2007

This study was conducted to investigate an aeration tank with RBC process attached Bacillus sp. known as a suitable microorganism for the removing of organic carbon, nitrogen and phosphorus. An aeration tank was based on tapered aeration because Bacillus sp. was well grown in this like environment conditions. The biofilm process with Bacillus sp. as an advanced treatment process could be a best technology for the prominent removal of organic carbon, nitrogen and phosphorus if the mechanism in the process is verified. The operation conditions of DO in the tapered aeration tank were maintained as $1.2{\sim}1.5mg/L$ in aeration tank1, as $0.3{\sim}0.5mg/L$ in aeration tank 2 and less than 0.2 mg/L in aeration tank 3, respectively. Lab-scale experiments were conducted, at room temperature, internal recycle rate was from 200% to 50% and returned sludge rate was from 100% to 50%. As a result, concentration of organic carbons, nitrogen and phosphorus in Period 1 (the time of Bacillus sp. adapted to environment) were decreased gradually. Ultimately, each removal rate in this biological experiment were TCODCr 94%, BOD 87%, T-N 85%, T-P 89% in Period 2. Hence, this process showed an excellent performance of the removal of organic carbon, nitrogen and phosphorus and this is an effective system fur treating of wastewater.

• Journal of Soil and Groundwater Environment
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• v.9 no.3
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• pp.49-55
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• 2004

A soil enrichment LYF-1 culture from a contaminated site, which could reductively dechlorinate 900 $\mu$M (ca. 150 mg/L) of tetrachloroethylene (PCE) stoichimetrically into cis-1,2-dichloroethylene (cis-DCE), was established and characterized. The enrichment culture can use yeast extract, peptone, formate, acetate, lactate, pyruvate, citrate, succinate, glucose, sucrose, and ethanol as electron donors for dechlorination of PCE. Addition of NO$_2$$^{[-10]}$ and NO$_3$$^{[-10]}$ as alternative electron acceptors showed complete inhibition of PCE dechlorination, but S$_2$O$_3$$^{-2}$ , SO$_3$$^{-2}$ and SO$_4$$^{-2}$ had no significant effect on PCE dechlorination. The enrichment culture was attached to ceramic media in an anaerobic fixed-bed reactor. The fixed-bed reactor showed more than 99％ of PCE degradation in the range of PCE loading rate of 0.13-0.78 $\mu$moles/L/hr. The major end product of PCE dechlorination was cis-DCE.

• Journal of the Korean Society for Library and Information Science
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• v.57 no.4
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• pp.5-24
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• 2023

In ancient Egypt, temples were not only religious sanctuaries but also community centers. One of the core spaces created in the temple is the facility where priests and scribes copied and preserved texts on papyrus and other media. Its common designation was 'pr-mḏȝ'(House of Books) and the 'per-(nw)-seshw'(House of Scrolls). The general term used during that time was 'Per Ankh', and the modern term for it is 'temple library'. Therefore, this study first identified the character and identity of the Per Ankh attached to the temple, and then traced whether it is appropriate to designate 'healing place of the souls' depicted on the hypostyle hall(Per Ankh) in the Ramesseum(mortuary temple) built by Ramses II of the New Kingdom as a library. As a result, Per Ankh, a hieroglyph combining the Per(house) and Ankh(life), was revealed to be a multi-purpose complex facility consisting of a learning and research center, a treatment and healing center with medical facilities and sanatoriums, a religious ceremony and a center for the celebration of eternal life, a scriptorium and a library. Therefore, the traditional argument that Per Ankh refers to a library cannot be justified. In the same context, the inscription 'Ψυχῆς ἰατρεῖον' on the doorplate of the hypostyle hall of the Ramesseum, which was first introduced by Greek historian Hecataeus of Miletus in the 4th century BC, was translated into Latin as 'Psychēs Iatreion' by Diodorus Siculus in the 1st century BC and described as the motto of the sacred library. However, Psyche is the goddess of Greek and Roman mythology, and Iatreion means hospital(clinic, healing center) and pharmacy, so Per Ankh in the Ramesseum is a space to heal the soul of the pharaoh (Ka). Therefore, 'Psychēs Iatreion = library' is a distortion and a mistranslation. It is not the motto of the library, but a metaphor for the Per Ankh.

• Information Systems Review
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• v.22 no.4
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• pp.163-184
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• 2020

The information on SNS, which is created by the forms of postings and comments, is being magnified and redistributed to news media expanding its impacts on real words. This amplifying effects of SNS comments have been increasingly discussed but there still lacks the answers for which dimensions of information is magnified, and what affects the direction and the degree of the amplification. This study, therefore, explores the detailed dimensions that are magnified by SNS comments and how SNS posting structure and social network characteristics affect them by using sentiment analysis. By analyzing 2,378 Facebook postings and news articles and their 26,312 SNS and 74,730 news site comments, this study shows that SNS comments magnify the sentiments of the posting articles they are attached to. In comparison to news site comments, SNS comments magnify the cognitive and social dimensions more than the news site comments. In the affective dimension, they tend to magnify only the positive emotion more than news site comments. Also, the findings reveal that whether the article in the posting is written by the posting owner affects the degree of amplification when the comments are remained positive or switched positive, while the opposite determines the amplification when comments remain negatively, suggesting that the user relationship in social network is the important factor that affects the direction and the degree of the information amplification in SNS.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.1
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• pp.49-60
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• 2008

Objective: This study was carried out to investigate the effects of 3-dimensional co-culture of human endometrial cells decidualized with progesterone and TGF-${\beta}1$ on the development of 2-cell mouse embryos. Methods: Stromal and epithelial cells isolated from human endometrial tissue were immunostained for cytokeratin and vimentin. Expression of TGF-${\beta}1$, its receptor-1, -2, integrin-${\beta}3$ and prolactin in mono or co-culture according to three different hormone conditions was investigated by RT-PCR. Differential staining was used to investigate the number of ICM and trophectoderm of hatched mouse blastocysts in different three conditions. Results: The immunohistochemical study was positive for cytokeratin or vimentin and confirmed that epithelial and stromal cells were isolated from endometrial tissue successfully. In co-culture, TGF-${\beta}1$, its receptor-1, integrin-${\beta}3$ and prolactin except TGF-${\beta}1$-r2 were expressed in progesterone dominant condition. The hatching and attaching rate were higher in the co-culture with decidualized cells (p<0.05). However, we observed that lots of the incomplete hatched blactocysts attached on non-decidualized cells. The ICM number of hatched mouse blastocysts was higher in co-culture with decidualized and non decidualized cells than media only culture (p<0.05). The trophectoderm number of hatched blastocyst was higher in the co-culture with decidualized cells than non-decidualized cells or media only culture (p<0.05). Conclusion: The administration of progesterone, estrogen and TGF-$\beta$ could induce decidualization of stromal and epithelial cells isolated from human endometrial tissue using 3-dimensional co-culture, and the decidualization of human endometrial cells could increase the hatching and attaching rate of 2-cell mouse embryos.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.3
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• pp.231-239
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• 2006

In this study, we evaluated the efficiency of using sulfur-$CaCO_3$ complex pellet in the sulfur oxidizing autotrophic denitrification process for synthetic wastewater with high $CaCO_3$ concentration. The sulfur-$CaCO_3$ complex pellet was packed in reactor(R4). Influent ${NO_3}^--N$ loading rate was from 200 to $1,000g/m^3{\cdot}day$. During the operation, average denitrification efficiency of R4 was above 95%. Particularly, the denitrififation rate at $1,000g/m^3{\cdot}day$ loading was 98.96% for R4. High ${NO_3}^--N$ removal efficiency was determined in R4 compared with other reactors. Through $Ca^{2+}$ and alkalinity analyses, we calculated the supplied alkalinity from the packed $CaCO_3$ in the reactor. Sulfur-$CaCO_3$ complex pellet more effectively supplied alkalinity through the dissociation of $CaCO_3$ as compared with other media. Based on these results, sulfur-$CaCO_3$ complex pellet increased the pH buffering capacity while also providing the carbon source to the denitrifying bacteria. Denitrification efficiency of R4 was also higher than other reactors. ESEM pictures of sulfur-$CaCO_3$ complex pellet show higher porosity than that of the granular sulfur. Hence, more denitrifying bacteria attached on the sulfur-$CaCO_3$ complex pellet than on granular sulfur. It can be concluded that the sulfur-$CaCO_3$ complex pellet is a more suitable media for a sulfur oxidizing autotrophic denitrification process as it provides high denitrification efficiency.

• Journal of Intelligence and Information Systems
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• v.26 no.3
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• pp.91-108
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• 2020

Korea's famous soccer players are steadily performing well in international leagues, which led to higher interests of Korean fans in the international leagues. Reflecting the growing social phenomenon of rising interests on international leagues by Korean fans, the study examined the overall consumer perception in the consumption of uniform by domestic soccer fans and compared the changes in perception following the transfers of the players. Among others, the paper examined the consumer perception and purchase factors of soccer fans shown in social media, focusing on periods before and after the recruitment of Heung-Min Son to English Premier League's Tottenham Football Club. To this end, the EPL uniform is the collection keyword the paper utilized and collected consumer postings from domestic website and social media via Python 3.7, and analyzed them using Ucinet 6, NodeXL 1.0.1, and SPSS 25.0 programs. The results of this study can be summarized as follows. First, the uniform of the club that consistently topped the league, has been gaining attention as a popular uniform, and the players' performance, and the players' position have been identified as key factors in the purchase and search of professional football uniforms. In the case of the club, the actual ranking and whether the league won are shown to be important factors in the purchase and search of professional soccer uniforms. The club's emblem and the sponsor logo that will be attached to the uniform are also factors of interest to consumers. In addition, in the decision making process of purchase of a uniform by professional soccer fan, uniform's form, marking, authenticity, and sponsors are found to be more important than price, design, size, and logo. The official online store has emerged as a major purchasing channel, followed by gifts for friends or requests from acquaintances when someone travels to the United Kingdom. Second, a classification of key control categories through the convergence of iteration correlation analysis and Clauset-Newman-Moore clustering algorithm shows differences in the classification of individual groups, but groups that include the EPL's club and player keywords are identified as the key topics in relation to professional football uniforms. Third, between 2002 and 2006, the central theme for professional football uniforms was World Cup and English Premier League, but from 2012 to 2015, the focus has shifted to more interest of domestic and international players in the English Premier League. The subject has changed to the uniform itself from this time on. In this context, the paper can confirm that the major issues regarding the uniforms of professional soccer players have changed since Ji-Sung Park's transfer to Manchester United, and Sung-Yong Ki, Chung-Yong Lee, and Heung-Min Son's good performances in these leagues. The paper also identified that the uniforms of the clubs to which the players have transferred to are of interest. Fourth, both male and female consumers are showing increasing interest in Son's league, the English Premier League, which Tottenham FC belongs to. In particular, the increasing interest in Son has shown a tendency to increase interest in football uniforms for female consumers. This study presents a variety of researches on sports consumption and has value as a consumer study by identifying unique consumption patterns. It is meaningful in that the accuracy of the interpretation has been enhanced by using a cluster analysis via convergence of iteration correlation analysis and Clauset-Newman-Moore clustering algorithm to identify the main topics. Based on the results of this study, the clubs will be able to maximize its profits and maintain good relationships with fans by identifying key drivers of consumer awareness and purchasing for professional soccer fans and establishing an effective marketing strategy.

• Clean Technology
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• v.12 no.2
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• pp.67-77
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• 2006

A simple dual sludge process, called as $KNR^{(R)}$ (Kwon's Nutrient Removal) system, was developed for small sewage treatment. It is a hybrid system that consists of an UMBR (Upflow multi-layer bioreactor) as anaerobic and anoxic reactor with suspended denitrifier and a post aerobic biofilm reactor, filled with pellet-like media, with attached nitrifier. To evaluate the stability and performance of this system for small sewage treatment, the pilot-scale $KNR^{(R)}$ plant with a treatment capacity of $50m^3/d$ was practically applied to the actual sewage treatment plant, which was under retrofit construction during pilot plant operation, with a capacity of $50m^3/d$ in a small rural community. The HRTs of a UMBR and a post aerobic biofilm reactor were about 4.7 h and 7.2 h, respectively. The temperature in the reactor varied from $18.1^{\circ}C$ to $28.1^{\circ}C$. The pilot plant showed stable performance even though the pilot plant had been the severe fluctuation of influent flow rate and BOD/N ratio. During a whole period of this study, average concentrations of $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, and TP in the final effluent obtained from this system were 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, and 0.87/0.17 mg/L (with/without poly aluminium chloride(PAC)), which corresponded to a removal efficiency of 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, and 55.4/90.3%, respectively. Excess sludge production rates were $0.026kg-DS/m^3$-sewage and 0.220 kg-DS/kg-BOD lower 1.9 to 3.8 times than those in activated sludge based system such as $A_2O$ and Bardenpho.