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Effects of 3-dimensional Co-culture of Human Endometrial Cells Decidualized with Progesterone and TGF-${\beta}1$ on the Development of Mouse 2-cell Embryos In Vitro  

Kwon, Wook-Hyun (Department of Obstetrics and Gynecology, School of Medicine, Pusan National University)
Kim, Hwi-Gon (Department of Obstetrics and Gynecology, School of Medicine, Pusan National University)
Lee, Dong-Hyung (Department of Obstetrics and Gynecology, School of Medicine, Pusan National University)
Ko, Kyung-Rae (Infertility Clinic, Pusan National University Hospital)
Lee, Kyu-Sup (Department of Obstetrics and Gynecology, School of Medicine, Pusan National University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.35, no.1, 2008 , pp. 49-60 More about this Journal
Abstract
Objective: This study was carried out to investigate the effects of 3-dimensional co-culture of human endometrial cells decidualized with progesterone and TGF-${\beta}1$ on the development of 2-cell mouse embryos. Methods: Stromal and epithelial cells isolated from human endometrial tissue were immunostained for cytokeratin and vimentin. Expression of TGF-${\beta}1$, its receptor-1, -2, integrin-${\beta}3$ and prolactin in mono or co-culture according to three different hormone conditions was investigated by RT-PCR. Differential staining was used to investigate the number of ICM and trophectoderm of hatched mouse blastocysts in different three conditions. Results: The immunohistochemical study was positive for cytokeratin or vimentin and confirmed that epithelial and stromal cells were isolated from endometrial tissue successfully. In co-culture, TGF-${\beta}1$, its receptor-1, integrin-${\beta}3$ and prolactin except TGF-${\beta}1$-r2 were expressed in progesterone dominant condition. The hatching and attaching rate were higher in the co-culture with decidualized cells (p<0.05). However, we observed that lots of the incomplete hatched blactocysts attached on non-decidualized cells. The ICM number of hatched mouse blastocysts was higher in co-culture with decidualized and non decidualized cells than media only culture (p<0.05). The trophectoderm number of hatched blastocyst was higher in the co-culture with decidualized cells than non-decidualized cells or media only culture (p<0.05). Conclusion: The administration of progesterone, estrogen and TGF-$\beta$ could induce decidualization of stromal and epithelial cells isolated from human endometrial tissue using 3-dimensional co-culture, and the decidualization of human endometrial cells could increase the hatching and attaching rate of 2-cell mouse embryos.
Keywords
Decidualization; TGF-${\beta}1$; Co-culture; Progesterone; RT-PCR;
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