• YAKHAK HOEJI
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• v.58 no.4
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• pp.255-261
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• 2014

Poly-pharmacy has been on the rise because of aging of population and chronic disease. Most of drug metabolism happens in the liver by CYP isozymes and the metabolism by CYP450 enzymes. The Cytochrome P450 (CYP) is a superfamily of enzymes that catalyzes the oxidations of many endogenous and exogenous compounds. Primary human Hepatocytes (HH) are considered as the gold standard model for In vitro drug interaction studies. However, there are several limitations (cost, limited life span) for using HH cells. HepaRG cells are being used as a possible alternative. HepaRG cells were cultured in William E medium containing the positive control inducers (1A2: 10, 25, 50 ${\mu}M$ omeprazole, 2C9 and 2C19: 10 ${\mu}M$ rifampin, 3A4: 10, 25, 50 ${\mu}M$ rifampin) at $37^{\circ}C$, 5 % $CO_2$ in a humidified atmosphere. This study was to evaluate the induction of CYP isozymes (1A2, 2C9, 2C19 and 3A4) using LC-MS/MS. We evaluated the potential induction ability of Bosentan, as a drug of pulmonary artery hypertension, in HepaRG cells. For reference, dose of the Bosentan is determined to the basis of the $C_{max}$ (835 mg/ml) value. The enzyme activity demonstrated that CYP2C9 and 3A4 were induced up to 20 times by Bosentan. Like as In vivo, the enzyme activity of CYP2C9 and CYP3A4 is significantly induced in a dose-dependent by Bosentan.

• Spatial Information Research
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• v.22 no.4
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• pp.49-58
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• 2014

Natural disasters of large scale such as typhoon, heat waves and snow storm have recently been increased because of climate change according to global warming which is most likely caused by greenhouse gas in the atmosphere. Increase of greenhouse gases concentration has caused the augmentation of earth's surface temperature, which raised the frequency of incidences of extreme weather in northern hemisphere. In this paper, we present spatial analysis of future typhoon genesis based on IPCC AR5 RCP 8.5 scenario, which applied latest carbon dioxide concentration trend. For this analysis, we firstly calculated GPI using RCP 8.5 monthly data during 1982~2100. By spatially comparing the monthly averaged GPIs and typhoon genesis locations of 1982~2010, a probability density distribution(PDF) of the typhoon genesis was estimated. Then, we defined 0.05GPI, 0.1GPI and 0.15GPI based on the GPI ranges which are corresponding to probability densities of 0.05, 0.1 and 0.15, respectively. Based on the PDF-related GPIs, spatial distributions of probability on the typhoon genesis were estimated for the periods of 1982~2010, 2011~2040, 2041~2070 and 2071~2100. Also, we analyzed area density using historical genesis points and spatial distributions. As the results, Philippines' east area corresponding to region of latitude $10^{\circ}{\sim}20^{\circ}$ shows high typhoon genesis probability in future. Using this result, we expect to estimate the potential region of typhoon genesis in the future and to develop the genesis model.

• Clean Technology
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• v.21 no.3
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• pp.191-199
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• 2015

The SO_x emission from the ship diesel engines will do a negative influence to the human health and the environment. To reduce the negative environmental effect of the SO_x emission caused by the high traffic of ship movements, the SECA (SO_x emission control area) has been set on several province around world to carry out the severe emissions control and to meet the emissions control standard. To cut down the SO_x emission from the ships, the wet type scrubber is being used widely. In this work, we prepared a numerical model to simulate the spray type scrubber to study the motion of liquid droplets in the flow of the scrubber. For the analysis, the CFD (computational fluid dynamics) method was adopted. As a special topic of the study, we designed the wave plate type of mist eliminator to check the carry over of the uncontrolled water droplet to the exhaust. Numerical analysis is divided into two stages. At the first stage, the analysis was done on the basic scrubber without the mist eliminator, and then the second stage of analysis was done on the scrubber with the mist eliminator on several condition to check and compare with the basic scrubber. On the condition of the basic scrubber, 42.0% of the distributed water droplets were carried over to the exhaust. But by adding the designed droplet eliminator at the exhaust of the scrubber, only 3.4% of the distributed water droplets supplied to the scrubber was emitted to the atmosphere.

• Management & Information Systems Review
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• v.17
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• pp.191-214
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• 2005

Recently, most enterprises are having a knowlege management boom. A number of books associated with the knowlege management are being published, countless public seminars are held, and many research councils have been organized studying it formally or informally as if not importing the system is like falling behind a fashion. However, there are not many cases that achieved success by constructing the system of knowledge management. Then, why the knowledge management is not so much effective despite so many voices wanting the change of management system and a lot of public lectures about it? I guess the reason is that most companies do not have concrete methodology. Seeing a result of a survey which reported that with spread of venture boom and successful examples being known widely, the outflow of precious human resources is accelerated and a large number of employees of conglomerates have already resigned or are considering separation from their positions, we can realize that are occurring a change which can be nearly called severance in an occupational view and an organization culture. The preference to a large enterprise or a public institution of labor is low today and the notion about a lifelong job is regarded as past remains. As for this, it could be said that the social atmosphere that pursued the stability of a job has been changed to the practical one that attaches importance to ability and pay. The way of thinking of employees has been changed while established organizations cannot satisfy their desire and this explains why important members of a company are flown out. The reason why superior human resources move to venture businesses is that they can do their likable work and also prove their ability as well as unconventional rewards. Although existing companies are trying to preserve important human resources through performance compensating stock option, temporary patching up of personnel management cannot retard the rushing wind of foundation and the outflow of labor. On the contrary, clumsy import of performance-based reward system not only fails to hire superior labor power but also can bring about a sense of incompatibility and conflicts among the remaining employees. Therefore, this thesis, focusing on how to choose, develop, and maintain the human resources, will suggest a future-aiming human resources management model of Korean enterprises after comparing and analyzing the actual condition of domestic companies and the trends of advanced corportaions.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.1
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• pp.94-98
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• 1985

A laboratory experiment was conducted to find out the denitrification rate upon the different levels of nitrogen fertilizer in submerged sandy soil. The results obtained were summarized as follows: 1. The highest denitrification rate was observed at 25 days after incubation. The amount was reached at 1830 ug/100g soil for 20mg nitrogen was applied in 100g soil. 2. Increases of fertilizer nitrogen was enhanced the rate of ammonification and nitrification during the incubation time. 3. Deep correlation was observed between the denitrification capacities which was determined as nitrous oxide and Mitchaelis-Menten kinetic with relation to nitrate concentration. More higher denitrification rates were observed in Mitchaelis-Menten kinetic than dentrification rate with determined as nitrous oxide. 4. A Zero order (with relation to nitrate concentration) kinetic model for denitrification was presented in this experiment condition to illustrate the variability of nitrous oxide concentrations in the submerged soil atmosphere.

• Clinical and Experimental Pediatrics
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• v.46 no.2
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• pp.167-172
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• 2003

Purpose : Pulmonary vascular hypertension is a common problem in congenital heart disease, the most common cardiac condition in childhood. However, the mechanisms responsible for this pathologic change, treatment, and prevention are poorly understood. Therefore, we studied the gene expression of vascular endothelial growth factor(VEGF) by using a hypoxic model of the pulmonary artery smooth muscle cells. Methods : The main pulmonary artery and its proximal branches of a 6 wk old Fischer rat were excised. They were cut into multiple small pieces and suspended in DMEM medium supplemented with 20% fetal bovine serum and incubated in 5% $CO_2$-95% air atmosphere. The smooth muscle cells were confirmed by immunostaining with smooth muscle myosin and ${\alpha}$-smooth muscle actin antibodies. The VEGF gene expression in the hypoxic group was compared with the one in control the group as well as the one in the starved group by RT-PCR and Northern blot hybridization. Results : There was no statistically significant difference among the control, hypoxic and starved groups. Conclusion : There are few studies of pulmonary vascular hypertension at the molecular level in Korea. Therefore, we studied the expression of VEGF gene in hypoxic pulmonary vascular smooth muscle cells. Further studies will be needed to find the difference between newly born and adult rats, or human and rat pulmonary vascular smooth muscle cells in gene expression. We hope that the study will lead to a better understanding of pulmonary vascular hypertension.

• Journal of the Korean Association of Geographic Information Studies
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• v.12 no.1
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• pp.73-80
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• 2009

Recently in order to mange better life quality much effort was spent for environmental-friendly urban development project and environmental restoration project. During these projects, there should be deep understanding about atmospheric environment change analysis and long term monitoring so that it would be helpful for better environment promotion such as heat island mitigation effect and wind way construction. In this study, the surface temperature environment change between before and after Cheonggye Stream Restoration Project was mapped and analyzed by using ASTER(Advanced Spaceborne Thermal Emission Reflection Radiometer) TIR(Thermal Infrared) satellite imagery and finally the fact, that the heat island effect was mitigated, was clarified. For this study, the correlation analysis was conducted through comparing the difference between atmosphere temperature of AWS(Automatic Weather System) and surface temperature of ASTER. Furthermore, this study will be the infrastructure of urban meteorology model development by understanding surface temperature pattern change and executing quantitative analysis of heat island.

• Journal of the Korean earth science society
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• v.39 no.2
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• pp.193-207
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• 2018

The purpose of this study was to analyze trends in conceptual change research in the field of Earth Science education conducted in Korea and to provide them as basic data of future concept change research. The trends of conceptual change research were analyzed in terms of keywords, year of publication, source of research, purpose of research, subject, area of research, and research method. This study was carried out on a total of 126 conceptual change papers of Earth Science education published from 1989 to July 2017. This study showed the following conclusions: First, the conceptual change research papers used more the term 'conceptual change' than 'misconceptions' and 'preconceptions'. Second, the conceptual change paper has achieved a large amount of growth in the 2000s. Third, conceptual change research tended to proceed to the subject of master's dissertation. Fourth, there were a lot of researches to find out the misconceptions and change them into the right concept, the conceptual change paper used free response test for misconceptions, and cognitive conflict instruction model and CAI (Computer Assisted Instruction) teaching strategy for misconceptions. Fifth, elementary school students were studied the most. Sixth, the study of conceptual changes in the field of Earth Science was high in the astronomical and atmospheric areas. Finally, although quantitative analysis methods were used in the early days of research, in the 2010s, qualitative analysis methods were widely used. In this paper, we proposed research on conceptual change for pre- and in-service teachers, expansion of research on conceptual changes in various fields such as geology, oceanography, atmosphere in Earth Science, investigation of causes of misconception in Earth Science and research on individual's affective factors, and so on.

• Journal of Fashion Business
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• v.13 no.1
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• pp.152-166
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• 2009

By virtue of the development of mass media, the cinema, the composite space art taking the visual and auditory elements together, exhibits the actual life of the realities, thereby having a mutually close relationship to social, cultural and economic fields and continuing to generate the fashion code as well as reflecting the image of the times. Especially, fashion style in movies delivers their image and atmosphere and becomes the means for containing the personality, spiritual world and inner thinking of the characters in the movie and inducing its plot. Therefore, this study was intended to make clear that fashion fuses and shares with a diversity of genres such as movies and the like, becomes the cultural model that proceeds to create a new culture in relation to daily life and induces and presents the trend of contemporary fashion. For this purpose, this study attempted to analyze fashion style in the movie. Lolita is the fiction published by the Russian?American writer Vladimir Nabokov($1899{\sim}1977$) in 1954. It is the fiction that portrays the unethical love between Humbert, a middleaged man, and Lolita, a girl in her 10s. It was cinematized by the director Stanley Kubrick for the first time in 1962 and revived by the movie director Adrian Lyne in 1997. The character of Lolita has a younger look like a girl and looks immature in the movie directed by the movie director Stanley Kubrick and the movie director Adrian Lyne. But the character of Lolita has the commonality that she showed an incomplete female image of having a sexually freewheeling thinking. Thereby, this study sought to prove that the created fashion style of the character in the film not only became the clue to enable us to know the time and space background in the film but also helped the film develop effectively by performing a role of portraying the character in the movie. And it attempted to present that it becomes both the foundation for leading the fashion trend shown in contemporary fashion and the code of mass culture. Fashion style of Lolita in the movie appears to be reflected diversely in mass culture as well as fashion style in the contemporary times.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.4105-4105
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• 2001

Near infrared spectroscopy (NIRS) was employed to qualify and quantify on survival, the injury rate and apoptosis of the human breast cancer cell line MCF-7 cells. MCF-7 cells were cultured in RPMI medium supplemented with 10% FCS in a 95% air and 5% CO2 atmosphere at 37$^{\circ}C$. For the viable cells preparation, cells were de-touched by 0.1% of trypsin treatment and washed with RPMI supplemented with 10% FCS medium by centrifugation at 1000 rpm for 3min. For the dead cells preparation, cells were de-touched by a cell scraper. The cells were counted by a hemacytometer, and the viability was estimated by the exclusion method with frypan blue dye. Each viable and dead cells were suspended in PBS (phosphate bufferred saline) or milk at the cell density desired. For the quantitative determination of cell death by measuring the LDH (lactate dehydrogenase) activity liberated from cells with cell membrane injuries, LDH-Cytotoxic Test Wako (Wako, Pure Pharmaceutical Co. Ltd., Japan) was used. We found that NIRS measurement of MCF-7 cells at the density range could evaluate and monitor the different characteristics of living cells and dead cells. The spectral analysis was performed in two wavelength ranges and with 1,4, 10 mm pathlength. Different spectral data pretreatment and chemometrics methods were used. We applied SIMCA classificator on spectral data of living and dead cells and obtained good accuracy when identifying each class. Bigger variation in the spectra of living cells with different concentrations was observed when compared to the same concentrations of dead cells. PLS was used to measure the number of cells in PBS. The best model for measurement of dead cells, as well as living cells, was developed when raw spectra in the 600-1098 nm region and 4 mm pathlength were used. Smoothing and second derivative spectral data pretreatment gave worst results. The analysis of PLS loading explained this result with the scatter effect found in the raw spectra and increased with the number of cells. Calibration for cell count in the 1100-2500 nm region showed to be very inaccurate.