• Title/Summary/Keyword: asparagine

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Physio-chemical studies on the after-ripening of hot pepper fruits -(Part 4) Changes in amino acids, organic acids and sugars- (신미종(辛味種)고추의 추숙(追熟)에 관(關)한 생리화학적(生理化學的) 연구(硏究) -제4보(第4報) Amino acids.유기산(有機酸).당(糖)의 변화-)

  • Lee, Sung-Woo
    • Applied Biological Chemistry
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    • v.14 no.1
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    • pp.43-50
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    • 1971
  • Measuring the changes of free amino acids, organic acids, free sugars, for after-ripening of the peel of hot pepper, the writer has obtained the following results; 1. Glutamine, asparagine, and glutamic acid as free amino acid of hot pepper are rich, while the total amount of free amino acids is greatly decreased through after-ripening. 2. The major organic acids of hot pepper is malic acid and citric acid, and their total amount comes to 80% of the total acid through the whole after-ripening period. Malic acid, however, is greatly decreased while citric acid is increased through after-ripening. And in the course of after-ripening the total amount of acid is decreasing, particularly with a sharp decrease in the post-cli. stage. 3. As free sugar in hot pepper, glucose is the major one, fructose, galactose and sucrose are identified, and existence of raffinose is presumed. Through after-ripening the total amount of free sugar is decreased about 25% in the post-cli. stage, and reducing sugars and non-reducing sugars also are decreased.

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Elution Profiles of Volatile Compounds and Free Amino Acids during Alcohol Soaking of Garlic(Allum sativum L.) (마늘의 alcohol 침지 중 휘발성 향기성분과 침출유리아미노산 함량)

  • Lee, Young-Guen
    • Journal of Life Science
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    • v.17 no.2 s.82
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    • pp.286-292
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    • 2007
  • Free amino acids and volatile compounds of fresh garlic and its liqueur were investigated to search elution profile of those components as basic data for development of garlic liqueur. The garlic was soaked in 20% alcohol solution and then sampled every week for 5 weeks. The major free amino acids were L-aspartic acid, L-glutamic acid, L-arginine, L-alanine, L-proline, L-asparagine and L-serine. Neutral amino acids such as L-threonine, L-proline, L-valine and L-leucine, and aromatic amino acids such as tyrosine and phenylalanine were eluted over 80% of those content in fresh garlic after 3 weeks of soaking, but acidic, basic and sulfur containing amino acids were below 80% even after 5 weeks. Sulfide compounds such as diallyl trisulfide, diallyl disulfide, methyl allyl disulfide, 2-vinyl-4H-1,3-dithi in, 3-vinyl-3,4-dihydro-1,2-dithiin, 3,5-diethyl-1,24-trithiolane, isobutyl isothiocyanate and diallyl sulfide were identified as major volatile compounds of fresh garlic by using GC/MS. Among volatile compounds of fresh garlic, allyl alcohol, diallyl disulfide, 3,5-diethyl-1,2,4-trithiolane, diallyl trisulfide and 3,4-dimethoxy furan were eluted to liqueur, but those compounds except 3,5-diethyl-1,2,4-trithiolane were lowered in liqueur during soaking. Furfural, 5-methylfurfural, 5-hydroxymethylfurfural, dimethyl pyrazine, furfuryl alcohol, 3-hydroxy-2-bytanone and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyr-an-4-one were generated newly and their content increased in liqueur during soaking.

Chemical Components of Gastrodia elata Blume Powder (천마 분말의 화학적 성분)

  • Kim, Hyeon-Ju;Chung, Shin-Kyo;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.7 no.3
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    • pp.278-284
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    • 2000
  • The chemical components in freeze dried Korean Gastrodia elata blume powder were analyzed. The proximate composition was composed of moisture(2.12%), crude protein (4.41%), crude fat(1.20%), crude ash(2.25%), crude fiber(4.60%) and N-free extract(85.15%). Free sugar was composed of glucose(1,314mg%), sucrose(1,081mg%) and fructose(869mg%). Total organic acid content was 2,095mg% and composed of succinic acid(1,238mg%), citric acid, malic acid, oxalic acid and fumaric acid in the order. Among the 11 fatty acids detected, linoleic acid was the most predominant one and unsaturated fatty acid was 80.55 area percent. 18 and 39 amino acids were detected from total and free amino acid, respectively. Arginine, asparagine, proline and sarcosine were the major components of free amino acid. The content of crude saponin and total phenol were 3,768mg% and 821mg%, respectively. Ascorbic acid(6.4mg%), vitamin B1(1.2mg%), vitamin B2(1.7mg%) and niacinamide(0.2m%) were detected as soluble vitamins of the powder. The content of vitamin A and vitamin E was 1.22mg% and 0.32%, respectively.

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Comparison of Free Amino Acids and Nucleotides Content in the Olive Flounder Paralichthys olivaceus Fed with Extruded Pellet (건조 배합사료로 사육한 넙치 어육의 유리아미노산 및 핵산 관련 성분의 비교)

  • Jang, Mi-Soon;Park, Hee-Yeon;Kim, Kang-Woong;Kim, Kyoung-Duck;Son, Maeng-Hyun
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.746-754
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    • 2011
  • This study analyzed the taste components of dorsal muscle and fin muscle collected from olive flounder cultured with extruded pellet (EP) compared with those of olive flounder cultured with raw fish moist pellet (MP) as a control. The olive flounder in this study were cultured for 10 months with either the formulated extrude pellets (FEP), commercial extruded pellets (CEP) or the MP feed, and the average weight was 1.15 kg. The major nucleotides and related compounds in the dorsal muscles of the fish fed with MP, CEP and FEP diets were AMP and IMP. The fin muscle of the olive flounder contained the largest amounts of IMP($5.91{\mu}mol/g$). The K value of the dorsal muscle in olive flounder fed with MP, CEP and FEP showed 9.63%, 9.83% and 5.84%, respectively. The free amino acids in the dorsal muscle of olive flounder showed significantly differences in the composition depending on the feed, but the distribution of free amino acids showed similar patterns. Among free amino acids, taurine showed the highest content in all the experimental groups, and the asparagine and citrulline contents were significantly higher in the groups fed with CEP and FEP than in the group fed with MP. The significant differences were observed in the contents of specific free amino acids in dorsal muscle and fin flesh depending on the feed for cultured olive flounder.

Amino Acid Nutrition on Growth and Reproduction of Potato Dry Rot Fusarium roseum ‘Sambucinum' Variants (감자 건부(乾腐) Fusarium roseum ‘Sambucinum’ 변이체(變異體)의 균사생장(菌絲生長) 및 분생포자(分生胞子) 형성(形成)에 미치는 아미노산(酸) 영양(營養))

  • Lee, Chang-Un
    • The Korean Journal of Mycology
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    • v.10 no.4
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    • pp.187-192
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    • 1982
  • The effects of amino acids on mycerial growth and sporulation of the decreased and increased pathogenicity variants of potato dry rot Fusarium roseum ‘Sambucinum' were as following. The increased pathogenicity variants Ic52 and Ic116 showed the trend of greater mycerial growth, marking up to 67mg on leucine, than the decreased pathogenicity variants Dc14 and Dc91, and produced markedly low conidia below $25{\times}10^{4}/ml$ or no sporulation. On methionine all the variants and the wild type showed the lowest growth recording 14mg to 20mg with no sporulation. Cystine similarly as glysine supported the poor growth 30mg to 19mg of the variants Dc14 and Dc91, respectively, and did not support the sporulation. The former amino acid like asparagine supported the growth of the variants Ic52 and Ic1l6 marking 47mg and 36mg, respectively. On all the eleven amino acids the variant Dc14 showed the trend of more mycerial growth and sporulation than the variant Dc91 which marked the exceptionally high sporulation $195{\times}10^{4}/ml$ on glutamic acid.

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Extractive Nitrogenous Constituents of Toha (Caridina denticulata denticulata, Freshwater Shrimp) and Jeotsaeu (Acetes japonicus , Seawater Shrimp) (토하(土蝦) 및 젓새우의 함질소(含窒素) 엑스성분(成分)에 관(關)한 연구(硏究))

  • Park, Choon-Kyu;Park, Jung-Nim
    • Korean Journal of Food Science and Technology
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    • v.28 no.6
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    • pp.1111-1118
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    • 1996
  • The extracts of toha, a kind of freshwater shrimp (Caridina denticulata denticulata) being cultivated in Naju district, Chonnam of Korea and jeotsaeu, a kind of seawater shrimp (Acetes japonicus) being caught in Shinan district, Chonnam of Korea, were analyzed for the taste constituents-extractive nitrogen, free amino acids, oligopeptides, nucleotides and related compounds, quaternary ammonium bases, and guanidino compounds-using specimens collected from December in 1994 to November in 1995. The content of extractive nitrogen. free amino acids, oligopeptides, nucleotides and related compounds in toha extract was higher than that in jeotsaeu extract. On the contrary, the amount of homarine, trimethylamine oxide and trimethlamine in toha extract was lower than that in jeotsaeu extract. But in case of creatine and creatine both species showed almost the same level. Arginine, lysine, glutamine, glutamic acid, phenylalanine, leucine, alanine, asparagine and glycine were the major amino acids in toha extract, while arginine, glutamic acid, proline, glutamine, lysine, leucine, glycine, alanine and taurine were the major amino acids in jeotsaeu extract. Methionine, glutamine, valine were almost the same level between the two species. As for nucleotides and related compound, AMP was the principal constituent in toha extract, and inosine was the principal constituent in jeotsaeu extract. In conclusion, the content of major nitrogenous constituents in toha extract was more abundant than that in jeotsaeu extract. So toha was more excellent raw material than jeotsaeu.

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Constituents of the DRIED TOMATO FRUITS(Lycopersicon esculentum, Mi Soo) (건조 토마토의 성분조성에 관하여)

  • Chung, Tae-Yung;Hayase, Fumitaka;Okitani, Akihiro;Kato, Hiromichi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.3
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    • pp.1-10
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    • 1987
  • For surveying of constituents concerning the flavor of the dried tomato fruits, nonvolatile components such as minerals, sugars, free amino acids, nucleotides and lipids were analyzed and determined. Potassium, calcium and phosphorus were the major conatituents of ashes, but trace amounts of cadmium and lead were determined. Glucose and fructose were the major constituents in sugars, and the latter slightly showed higher value than the former. The content of total free amino acids was 8322.5mg%, and that of the major components such as asparagine, aspartic acid, urea. glutamic acid, ${\beta}-alanine$ and ${\gamma}-aminobuthyric$acid showed 58.01% to the total amount. The nucleotides were composed of CMP, UMP, GMP and IMP, and CMP among them showed the highest value as about 58.07% to the total amount. On the other hand, IMP showed the lowest one. The major components of the total fatty acids from the saponifiable fraction in the lipids were $C_{18:2}$, $C_{18:1}$ and $C_{16:0}$ and those of the total sterols from unsaponifiable one were stigmasterol and ${\beta}-sitosterol$.

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Quality Characteristics of Hwanggeumju as a Traditional Home-Brewed Liquor (전통 가양주인 황금주의 품질 특성)

  • Baek, Seong Yeol;Kim, Joo-Yeon;Baek, Chang Ho;Choi, Ji-Ho;Choi, Han-Seok;Jeong, Seok-Tae;Yeo, Soo-Hwan
    • Food Science and Preservation
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    • v.20 no.1
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    • pp.127-133
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    • 2013
  • The quality characteristics of Hwanggeumju (a type of traditional Korean rice wine), which has been described in an ancient document (Sangayorok), were investigated. During its fermentation, its pH gradually decreased from 4.23 to 3.96); and after four days of fermentation, its sugar content significantly decreased. After seven days, its alcohol content rapidly increased to 15.8 percent. Its major organic acid is lactic acid, but malic, succinic, citric and acetic acid were also detected in it. The free amino acid analysis showed relatively high alanine, arginine, asparagine, glutamic acid and leucine contents. The sensory evaluation resulted in high scores for color, turbidity and taste.

Quality characteristics of Hijikia fusiforme extracts with different extraction method (추출방법에 따른 톳 추출물의 품질특성)

  • Kwon, Yu-Ri;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.22 no.1
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    • pp.70-77
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    • 2015
  • The physiological properties of water extracts from Hizikia fusiformis extracted using different extraction methods (water extraction, WE; autoclave extraction, AE; high pressure extraction, HPE) were investigated. The freeze-dried powder yields from HPE, AE and WE were 29.33, 27.84 and 23.63%, respectively. The $L^*$ and $b^*$ color values were higher in WE, while the $a^*$ color values were higher in WE and AE. The total sugar content of AE (60.14%) was higher than those of WE (47.10%), HPE (40.97%). The reducing sugar content (7.88%) and protein content (42.83%) of AE was higher than those of WE, and HPE. The uronic acid (5.04%), total free amino acid (785.19 mg/g), taurine (19.16 mg/g), aspartic acid (66.63 mg/g), asparagine (204.84 mg/g), alanine (188.87 mg/g) and ammonium chloride (243.91 mg/g) contents, however, were the highest in HPE. Additionally, the crude polysaccharide yield was higher in HPE (4.75%) than in AE and WE, and the crude saccharide (fucose, galactose, glucose, xylose and fucose) yields were higher in AE. It can be concluded that optimum conditions for the efficient extraction of Hizikia fusiformis depending on components are high pressure and a lower temperature than in the typical process.

Chemical Analysis on Biologically Active Substances among Habitats of Allium victorialis for a High Income Crop (산마늘의 고소득 작물화를 위한 기능성 물질 분석)

  • 박희준
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.51-59
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    • 1998
  • When the contents of the constituents such as total amino acids, free amino acids, volatile organosulfuric compounds and steroidal saponins among three origins in the aerial-and underground parts of Allium victorialis, it was suggested that the characteristic components regarding to quality evaluation could be differed according to the purpose of utilization. For the utilization of amino acids, underground parts of this plant was shown to be better than aerial part. In addition, Ulung island origin was found to contain the highest amino acids content among the three origins though the difference was small. The amino acids showing remarkably high contents were appeared to be arginine, glutamine and asparagine. In the volatile organosulfuric compounds, the origina of Mt. Odae and Mt. Chiri positioned in inland showed higher contents than Ulung island origin geographically positioned in the ocean. Inland origins were shown to contain higher organosulfurie component contents in aerial parts than in underground parts while those of Ulung island origin were higher in underground parts than aerial parts. Underground parts, regarding to saponin constituents, showed higher contents than aerial parts. Underground parts of Ulung island origin were shown to contain more saponins than those of other two origins and the sequence of the contents was in the order of Ulung island>Mt. Chiri>Mt Odac.

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