DOI QR코드

DOI QR Code

Quality characteristics of Hijikia fusiforme extracts with different extraction method

추출방법에 따른 톳 추출물의 품질특성

  • Kwon, Yu-Ri (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu)
  • 권유리 (대구가톨릭대학교 식품공학전공) ;
  • 윤광섭 (대구가톨릭대학교 식품공학전공)
  • Received : 2014.10.01
  • Accepted : 2014.12.19
  • Published : 2015.02.28

Abstract

The physiological properties of water extracts from Hizikia fusiformis extracted using different extraction methods (water extraction, WE; autoclave extraction, AE; high pressure extraction, HPE) were investigated. The freeze-dried powder yields from HPE, AE and WE were 29.33, 27.84 and 23.63%, respectively. The $L^*$ and $b^*$ color values were higher in WE, while the $a^*$ color values were higher in WE and AE. The total sugar content of AE (60.14%) was higher than those of WE (47.10%), HPE (40.97%). The reducing sugar content (7.88%) and protein content (42.83%) of AE was higher than those of WE, and HPE. The uronic acid (5.04%), total free amino acid (785.19 mg/g), taurine (19.16 mg/g), aspartic acid (66.63 mg/g), asparagine (204.84 mg/g), alanine (188.87 mg/g) and ammonium chloride (243.91 mg/g) contents, however, were the highest in HPE. Additionally, the crude polysaccharide yield was higher in HPE (4.75%) than in AE and WE, and the crude saccharide (fucose, galactose, glucose, xylose and fucose) yields were higher in AE. It can be concluded that optimum conditions for the efficient extraction of Hizikia fusiformis depending on components are high pressure and a lower temperature than in the typical process.

본 연구에서는 다양한 추출방법(열수, 가압가열, 초고압 추출방법)을 통해 톳 추출물을 제조하였으며 품질특성을 실시하여 효과적인 톳 추출방법을 제시하고자 하였다. 추출 수율은 초고압 추출물, 가압가열 추출물, 열수 추출물 순으로 높은 수율을 확인하였으며 색도의 경우 열수 추출물에서 높은 명도와 황색도를 나타내었고 가압가열 추출물에서는 적색도가 높았다. 추출방법에 따른 톳 추출물의 전당 함량을 측정한 결과, 전체적으로 모든 추출물이 40~60%의 높은 전당 함량을 보였으며 특히 가압가열 추출물의 함량이 높았다. 또한 시료별 전당 함량의 경우 가압가열, 열수, 초고압 추출물의 순으로 높은 함량을 보였으며 이는 환원당과 단백질 함량에서도 동일한 결과를 나타내었다. 그러나 우론산 및 총 아미노산 함량의 경우 초고압 추출물이 다른 추출물보다 높은 함량을 보였으며, 특히 asparagine의 경우 초고압 추출물에서만 검출되었다. 톳의 다당류 성분을 알아보고자 에탄올을 이용하여 조다당류를 추출한 결과 수율의 경우 초고압 추출물에서 4.75%로 높았으며 가압가열, 열수 추출물의 순으로 나타났다. 또한 high performance anion exchange chromatography를 통하여 조다당류의 구성당을 살펴본 결과 fucose가 주 당성분인 polysaccharide임을 알 수 있었으며 galactose, glucose, xylose가 함유되어 있음을 알 수 있었다. 이러한 결과를 종합해 볼 때 높은 온도와 압력을 가해 추출할 경우 해조류의 수율 및 유용성분의 추출이 용이해짐을 알 수 있었으며 추후 적절한 압력과 온도 설정관련 연구를 통해 해조 가공산업에 유용할 것으로 판단된다.

Keywords

References

  1. Baek G, Goo BG, Ahn BJ, Park JK (2013) Effects of water-soluble polysaccharides from Tott on lipid absorption and animal body weight. J Korean Soc Food Sci Nutr, 42, 556-562 https://doi.org/10.3746/jkfn.2013.42.4.556
  2. Kim JH, Song HS, Yang JY (2012) Nutritional characteristics of Kochujang added with fermented extracts of Hizikia fusiforme. J Food Hyg Safety, 27, 473-478 https://doi.org/10.13103/JFHS.2012.27.4.473
  3. Kim SJ, Moon JS, Kang SG, Jung ST (2003) Extraction of porphyran from decolored laver. Korean J Food Sci Technol, 35, 1017-1021
  4. Song BB, Kim SK, Jeong GT (2011) Enzymatic hydrolysis of marin algae Hizikia fusiforme. Korean Soc Biotechnol Bioeng J, 26, 247-351
  5. Ponce NMA, Pujol CA, Damonte EB (2003) Fucoidans from the brown seaweed Adenocystis utricularis : extraction methods, antiviral activity and structural studies. Carbohydr Res, 338, 153-165 https://doi.org/10.1016/S0008-6215(02)00403-2
  6. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY (2004) Effect of ultrasonification process on enhancement of immuno-stimulatory activity of Ephedra sinica Stapf and Rubus coreanus Miq. Korean J Biotechnol Bioeng, 19, 113-117
  7. Kwon YR, Youn KS (2012) Quality and antioxidan characteristics of granule tea prepared with sea tangle (Laminaria japonica) and sea mustard (Undaria pinnatifida) powder as affected by extraction method. Korean J Food Preserv, 19, 525-531 https://doi.org/10.11002/kjfp.2012.19.4.525
  8. Hwang IG, Woo KS, Jeong HS (2011) Biological activity and heat treatment processing of foods. Food Sci Ind, 44, 56-65
  9. Jeong HS, Han JG, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH, Choi GP, Park UY, Lee HY (2009) Enhancement of anticancer activities of Ephedra sinica. Angelica gigas by ultra high pressure extraction. Korean J Medicinal Crop Sci, 17, 102-108
  10. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH, Lee HY (2007) Toxicity reduction and improvement of anticancer activities from Rhodiola sachalinensis A. Bor by ultra high pressure extracts process. Korean J Medicinal Crop Sci, 15, 411-416
  11. Lee GJ, Choi SD (2008) Application of biological industry using high hydrostatic pressure (HHP) system. Korean J Biotechnol Bioeng, 23, 362-368
  12. Saha SK, Brewer CF (1994) Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glyco-proteins using the phenol - sulfuric acid method. Carbohydr Res, 254, 157-167 https://doi.org/10.1016/0008-6215(94)84249-3
  13. Lowry OH, Rosebrough NJ, Farr AI, Randall RJ (1954) Protein measurement with the Folin phenol reagents. J Biol Chem, 193, 265-275
  14. Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem, 4, 333-334
  15. Park SJ, Choi YB, Ko JR, Kim YE, Lee HY (2014) Enhancement of antioxidant activities of blueberry (Vaccinium ashei) by using high-pressure extraction process. J Korean Soc Food Sci Nutr, 43, 471-476 https://doi.org/10.3746/jkfn.2014.43.3.471
  16. Choi WY, Lee HY (2014) Enhancement of antioxidant activities of Curcuma longa leaves by ultra high pressure extraction. Korean J Medicinal Crop Sci, 22, 121-126 https://doi.org/10.7783/KJMCS.2014.22.2.121
  17. Na HS, Kim JY, Park JS, Choi GC, Yang SI, Lee JH, Cho JY, Ma SJ (2014) Characteristics of marine algae extracts using subcritical water extract method. Korean J Food Preserv, 21, 62-68 https://doi.org/10.11002/kjfp.2014.21.1.62
  18. Fang Z, Sato T, Smith-Jr RL, Inomata H, Arai K, Kozinski JA (2008) Reaction chemistry and pahse behavior of lignin in high-temperature and supercritical water. Bioresour Technol, 99, 3424-3430 https://doi.org/10.1016/j.biortech.2007.08.008
  19. Koo SY, Cha KH, Lee DU (2007) Effects of high hydrostatic pressure on foods and biological system. Food Sci Industry, 40, 23-30
  20. Zhang S, Zhu J, Wang C (2004) Novel high pressure extraction technology. Int J Pharm, 278, 471-474 https://doi.org/10.1016/j.ijpharm.2004.02.029
  21. Lahaye M (1991) Marine algae as sources of fibers : determination of soluble and insoluble dietary fiber contents in some sea vegetables. J Sci Food Agric, 54, 587-594 https://doi.org/10.1002/jsfa.2740540410
  22. Grid JP, Marion C, Liutkus M, Boucard M, Rechencq E, Vidal JP, Rossi JC (1988) Hypotensive constituents of marine algae. 1 Phamacological studies of laminine. Plantamed, 54, 193-196
  23. Jung BM, Ahn CB, Kang SJ, Park JH, Chung DH (2001) Effects of Hizikia fusiforme extracts on lipid metabolism and liver antioxidative enzyme activities in triton-induced hyperlipidemic rats. J Korean Soc Food Sci Nutr, 30, 1184-1189
  24. Lee SH (2011) Current status and prospect of nutraceuticals from marine algae. Bull Food Technology, 24, 165-175
  25. Lee SD, Lee KS, Okuda H, Hwang WI (1990) Inhibitory effect of crude acidic polysaccharide of Korean ginseng on lipolytic action of toxohormone-L from cancerous ascites fluid. Korean J Ginseng Sci, 14, 10-13
  26. Jee HK, Cho YJ, Kim CT, Jang YS, Kim CJ (2006) Increase of solubility of Ginseng Radix by extrusion cooking. Korean J Food Sci Technol, 38, 361-368
  27. Choi YJ, Hwang KH (2011) Analysis of the extraction condition of soluble acidic polysaccharides from Ginseng Marc. Kor J Pharmacogn, 42, 82-88
  28. Park NY, Kim JS, Jeong YJ (2008) Effects of extraction conditions on the componential extraction of brown seaweed (Undaria pinnatifida). J Korean Soc Food Sci Nutr, 13, 321-326 https://doi.org/10.3746/jfn.2008.13.4.321
  29. Ministry of agriculture and forestry (2012) Material of Korean food Jeotgal fuctional investigation study. Republic of Korea
  30. Noda N (1993) Health benefits and nutritional properties of nori. J Appl Phycol, 5, 225-258 https://doi.org/10.1007/BF00004022
  31. Oishi K, Tamura Y, Kanai E (1961) Quality of Kombu, edible seaweeds belonging to the Laminariaceae. IV. Relation between quality and amino acid composition of extractives. Nippon Suisan Gakkaishi, 27, 601-605 https://doi.org/10.2331/suisan.27.601

Cited by

  1. Processing and Quality Characteristics of Glutinous Barley Gruel Containing Hizikia fusiformis vol.49, pp.3, 2016, https://doi.org/10.5657/KFAS.2016.0310
  2. Solubilization of Polysaccharide and Functional Components by High-pressure Enzyme Treatment from Ginger (Zingiber officinale Rosc.) vol.22, pp.2, 2018, https://doi.org/10.13050/foodengprog.2018.22.2.173
  3. 추출용매에 따른 톳(Hizikia fusiformis) 추출물의 항산화 및 생리활성 비교 vol.53, pp.6, 2015, https://doi.org/10.5657/kfas.2020.0886