• Journal of fish pathology
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• v.6 no.2
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• pp.205-218
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• 1993

On the development of hirame(Paratichtys olivaceus) culture, outbreak of scuticociliata infection was reported to cause severe damage in Japan. To establish effective measures for isolation and cultivation of this ciliate, we tried to culture this pathogenic ciliate using medium for bacteria and fish cell lines in vitro. Scuticociliata from the brain tissues of infected fish was aseptically inoculated to CHSE-214 cells cultured in MEM-10 without antibiotic. Scuticociliata grew well and the number of ciliate reached $10^6\;cells/ml$ at temperatures of $15^{\circ}C$ to $20^{\circ}C$ for 10d. The number of ciliate cultured in the cell lines is 10 times higher than the numbers cultured in the liquid medium alone. This ciliata could be cloned by dilution method. Scuticociliata isolated could grow well on 42 different cell lines that were established from marine fish, warm freshwater fish, and salmonids. This ciliate could be preserved in liquid nitrogen for more than 6 months. Subsequently, we observed the optimal temperature and salinity for growth, and tested the sensitivities of this organism to formaldehyde, flagyl(Metronidazole), Ekuteshin(Combination compound of sulfamonometoxin and ormethoprim), and ozonixation. Optimal temperature for growth was $25^{\circ}C$ and salinity was 1.0 to 1.5%. Washed scuticociliata was killed by formaldehyde at the concentration of 50ppm for 10min, but was not completely killed even at a high concentration of 400ppm for 20min in MEM-5. Flagyl and Ekuteshin can inhibit the growth of scuticociliata at the concentration of 1,000 and $100{\mu}g/ml$ in MEM-10, respectively. More than 99% of this scuticociliata could be killed by ozonization at a dose equivalent to $1.0mg/\ell$ oxidant for 30sec in sea water. Isolated scuticociliata showed the pathogenicity to the cultured hirame by artificial infection(I. P. injection, $10^5\;cells$/fish). The number of scuticociliata in the water could be counted by most probable number(MPN) method using tissue culture, and the minimum detectable number was $1.8\;cells/\ell$. The number in the reservoir tank for water supply to the culture tank was 110 cells/l. After cleaning by elimination of the sediments from of the reservoir tank and disinfected with formaldehyde, number of scuticociliata decreased and was counted less than $1.8\;cells/\ell$ and infection rate of cultured hirame was decreased.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.11
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• pp.2025-2036
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• 2000

A physicochemical process called ultra rapid coagulation(URC) was applied as a pilot scale to recover eutrophicated lake water at Inkyung Lake located on-campus in Inha university. The URC was uniquely designed to completely remove the presence of phosphorus up to 95%, which in turn leading to lessen the level of eutrophication of lake. For a pilot test of period, the lake was restored showing Carlson's Trophic State Index(TSI) of mesotrophic state which accounts for the reduction of total phosphorus and chlorophyll-a down to approximately 55 from 70 and 73, respectively. A residual presence of aluminum and additional coagulants in the effluent was tested for their potential effects on Photobacterium phosphoreum. The effect concentration ($EC_{50}$-15 min) observed in the effluent revealed that the bioactivity of Photobacterium phosphoreum was not influenced by the residual presence of coagulants. After the pilot test of period, the lake was consequently restored as a mesotrophic state in obtaining the second grade of lake water quality. The URC may be further applied for restoration of greater scale of lake in eutrophication.

• Journal of the Korean earth science society
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• v.31 no.6
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• pp.608-624
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• 2010

Morphological surveys of southern Gyeonggi Bay in the Yellow Sea were conducted for2 years (2006 and 2007) by using multibeam echosounder for investigating the morphological features of bedforms. The subaqueous dunes are shown in various shapes (A~F type) and continuous spectrum of heights and lengths of transverse-to-current dunes on the wide range of sedimentary types. The height-length power-law correlation of dunes is $H_{mean}=0.0393L^{0.8984}$ (r=0.66). The comparison between Flemming (1988)'s correlation and height-length correlation of this study indicates that the subaqueous dunes in the study area are equilibrated in the present hydrological and sedimentary environment. The major controlling factors to thedevelopment and maintenance of subaqueous dunes are both strong tidal currents and the abundant availability of sand. Marine sand mining, artificial impact, changes from the original shape to an irregular shape of the subaqueous dunes with a shorter wavelength and lower height, which has influence on the development and maintenance of bedform because it causes a decrease of the availability of sediment. Water depth and sedimentary characteristics, and othercontrolling factors seem to play limited roles in the development and maintenance of subaqueous dunes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.4
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• pp.497-502
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• 1996

Artificial fertilization of ice fish, Mypomesus transpaciticus nipponensis caught at Milyang-river and Osib-chun brook was performed in March 24, 1990, and the hatched larvae were reared for 25 days to describe the development of eggs and larvae. Fertilized eggs were spherical in shape, measuring $0.85\~1.05\;mm$ in diameter (mean: 0.97 mm) and translucent adhesive with many small-sized oil globules on the surface. Hatching in the indoor tank started from the 170 hours after fertilization under $16.5^{\circ}C$ water temperature. Newly-hatched larvae were measured $3.85\~4.25\;mm$ in total length (mean: 4.05 mm), and mouth and anus were not yet open. They had one yolk sac on the anterior part of abdomen, straight-type's notochord, and $52\~54$ myotomes. The larva of 5 days old transformed to postlarval stage and measured $5.20\~5.65\;mm$ (mean: 5.37 mm) in total length. As the yolk sac was completely absorbed, mouth and anus were open, and they fed rotifers vigorously. In 20 days after hatching, the larvae grew to 8.38 mm in TL, and the caudal notochord flex at $45^{\circ}$. In 25 days after hatching, total length reached 9.63 mm. The pan of the fin-fold of the future dorsal and anal fins became high.

• Journal of Korean Society of Forest Science
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• v.89 no.2
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• pp.145-160
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• 2000

This study is focused to the national park of Korean typical Sea Hally$\check{o}$ Haesang, and its visual resources and practiced inspect course by the way of suppositions and tests, to show the visual resource management objectively, and that of qualitative basic data. Accordingly by measuring the physical amount spatial structure with the visual amount originated from the Mesh Analyzing Method and the Visual Preference from the Scenic Beauty Estimation(S.B.E.) method and analyzed the valuation of the visual resource by Iverson method. Spatial image structure measured by Semantic Differential(S.D.) Scale was shown through the factor analysis algorithm for the analyzing psychological amount and examined the flowing out of decisive factor and the objective importance related to the mutual factors by appling the measurement of the visual quality. As a national Park, the visual factors that have natural landscape harmonized with forest, sky, surface of the water, curious stones and rocks, and temples should be escalated their values affirmatively so as to be the scenery of pointed direction and enjoyable, and it is of more needed for visual resource and its' controlling technique to make artificial structures more intentional planning and systemical setting. When we are viewing the improvement for the national park along with the visual resource management, reasonable level of development is needed, because when men interference surpass plantations and leasts will be damaged and the quality of natural landscape can be lowered, so it is needed to set up a management end, tangibly or clearly; and it is permitted limit coming and going ablably by accounting the suitable number for availing. But the controling end should be set in every level, positive management, very actively within the permissive varcability. It is the main business for the national park to prevent the damage from human for their gay life or to prevent the damage of a land carpet, and to restorate for the visual resource management.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.3
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• pp.27-27
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• 1987

The purpose of this study is to find the light intensity which induced maximum gathering rate and to observe the variation of the gathering rate both at daytime and night by using Sea-bass, Epinephelus septemfasciatus (Thunberg). An experimental tank (360 L×50 W×55H cm) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Eight artificial light sources were prepared by combination of three with white light bulbs (10W, 60W, 100W) and eight filters. During the experiment water depth was maintained 50cm level in the tank. The tank was marked into six longitudinal sections each being 60cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes before the main experiment. Upon turning on the light, the number of fish in each section was counted 60 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 24.13 lux (15.25~35.93 lux) at daytime and 0.41 lux (0.25~0.63 lux) at night. The variation of the gathering rate of fish in illumination time was great and did not show any definite pattern but fluctuated irregularly. Its different between daytime and night is remarkable.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.3
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• pp.137-143
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• 1987

The purpose of this study is to find the light intensity which induced maximum gathering rate and to observe the variation of the gathering rate both at daytime and night by using Sea-bass, Epinephelus septemfasciatus (Thunberg). An experimental tank (360 L$\times$50 W$\times$55H cm) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Eight artificial light sources were prepared by combination of three with white light bulbs (10W, 60W, 100W) and eight filters. During the experiment water depth was maintained 50cm level in the tank. The tank was marked into six longitudinal sections each being 60cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes before the main experiment. Upon turning on the light, the number of fish in each section was counted 60 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 24.13 lux (15.25~35.93 lux) at daytime and 0.41 lux (0.25~0.63 lux) at night. The variation of the gathering rate of fish in illumination time was great and did not show any definite pattern but fluctuated irregularly. Its different between daytime and night is remarkable.

• Journal of the Korean earth science society
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• v.23 no.7
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• pp.565-574
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• 2002

Based on previous investigations of aerial photographs and topographical surveys, this study focuses on the sedimentologic features of the Daebudo area including sedimentation processes, sedimentary facies and hydrologic conditions of the erosional coast. A total of 137 surface sediments and one core (by hand auger) sediment were obtained to interpret the depositional environment of the erosional coast in the macro-tidal coast. Surface sediments are distributed from sandy gravel (sG) to silt (Z). Textural parameters are characterized not only by coarse, poorly sorted, positive skewed and multi-modal distribution in the supra-tidal flat, but also finer, relatively well-sorted, symmetric distribution in the intertidal flat. According to the C/M diagram, sediment transport modes of study area are characterized by the mixed mode of suspension and bedload in the upper-, middle-tidal flat and by uniform suspension in the lower-tidal flat due to tidal effect. Vertical sediment distribution of the core, collected near shoreline, shows coarsening-upward, poorly sorted pattern by the input of detritus resulting from coastal erosion. Considering the sedimentological features of the study area, it appears to be composed of a coastal zone changed by not only artificial reclamation, but also by natural processes such as strong wave action due to typhoons and storms during high water level and long/short-term sea level rising. As a result, tide-dominated erosional coasts show that the shore is affected by local, temporal and hydrological conditions near high tide level and that the intertidal flat is represented by a general tide-dominated sedimentary environment.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.225-236
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• 2003

In order to understand the relationship of depositional environment between fluvial and estuarine-embayment in Han River system, including depositional change in main Han River, more than 250 bottom sediment and 70 suspended sediment were analyzed with hydrologic data. Based on the previous data, the study area can be divided into two environment(fluvial and estuarine-embayment) by Singok underwater dam. The gravelly facies occurs in the South and North Han Rivers and sandy and silty facies occupies in the main Han River. Depositional environment of main Han River changed mainly because of limited sediment transport and hydrological condition. In the estuarine-embayment environment, coarse-grained sediments are dominant in tidal channel and of shore whereas fine and poorly sorted sediments are observed in coastal area. During moderate period, relationship between fluvial-estuarine-embayment system is discontinuou s because of flow restriction by artificial construction such as dam and underwater dam, so that each river system characterizes the individual environment. Fluvial and estuarine system is influenced by tide and, thus, transition zone of estuarine- embayment system moves landward. During flooding period, however, each river system is integrated as continuous depositional system by high discharge and, thus, transition zone of fluvial-estuarine-embayment system moves seaward. For further detailed systems about the lower Singok under-water dam, joint research of South-North Korea should be necessary.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.2
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• pp.153-159
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• 2016

Gwangyang traffic safety designated area is composed of 3 fairways (Deep water fairway, inbound fairway, and outbound fairway). However, inbound vessels can't use this passage because of Samyeo rock and artificial fishing banks in inbound route. The problem with the rocks and artificial fishing banks has been raised by ship navigators and authorities of the port. This research is about the safety evaluation and management plan of the passage, and we conducted maritime traffic simulation using a model based on a ship operator risks. As a result, assuming that future marine traffic volume is the same as the present, and if the ship operators use 3 fairways and not two, it showed risk reduction of 46.4 % (vessels over 50,000 DWT using DW route) and 57.1 % (vessels over 10,000 DWT using DW route). Also, in a traffic volume condition which is the same as the present, to induce vessels over 50,000 DWT to use DW route is effective in mitigating of risks. Meanwhile, in a condition which increased the traffic volume by 150 %, it is more effective to induce vessels to use DW route. This research is the result of analysis using the model based on ship operator risks, and not cost-effectiveness analysis on the removal of Samyeo rock and artifical fishing banks. This research is expected to be used on setting up the sea route and management plan (particularly, restriction on passing DW route).