• Mycobiology
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• 제35권4호
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• pp.235-237
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• 2007

Leaf blight and bulb rot symptoms were observed on narcissus plants grown in Yongin, Cheongwon and Namhae areas in Korea during disease survey from 1999 to 2002. A total of 15 isolates of Botrytis sp. were obtained from the infected plant parts of narcissus. All the isolates were identified as Botrytis narcissicola based on their morphological and cultural characteristics. Three isolates of B. narcissicola were tested for their pathogenicity to leaves and bulbs of narcissus by artificial inoculation. All the isolates induced leaf blight and bulb rot symptoms on the plants of narcissus by artificial inoculation. The symptoms induced by artificial inoculation were similar to those observed in the fields. This is the first report of narcissus smoulder caused by B. narcissicola in Korea.

• Mycobiology
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• 제35권4호
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• pp.238-240
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• 2007

Anthracnose symptoms often occurred on fruits of Asian pear trees grown in Anseong, Naju, Seonghwan and Pyeongtaek in Korea during the harvesting period from 2000 to 2005. A total of 28 isolates of Colletotrichum sp. were obtained from the anthracnose symptoms. All the isolates were identified as Colletotrichum acutatum based on their morphological and cultural characteristics. Four isolates of the fungus were tested for pathogenicity to fruits of Asian pear tree by artificial inoculation. All the isolates induced anthracnose symptoms on the fruits by wound inoculation but not by unwound inoculation. The anthracnose symptoms induced by artificial inoculation were similar to those observed in the orchard. This is the first report of anthracnose of Asian pear tree caused by Colletotrichum acutatum.

• The Plant Pathology Journal
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• 제37권5호
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• pp.455-464
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• 2021

Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of Fusarium graminearum by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of F. graminearum by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (r = 0.47, P < 0.01) and FHB incidence (r = 0.56, P < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (r = 0.12, P > 0.05) under natural infection and it was not correlated with DON concentration (r = -0.23, P > 0.05) and FHB incidence (r = 0.19, P > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.

• Journal of Forest and Environmental Science
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• 제29권2호
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• pp.173-180
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• 2013

Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.69.2-70
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• 2003

For the selection and breeding of chestnut varieties resistant to the chestnut blight fungus Cryphonectria parasitica, disease resistance and susceptibility of 28 varieties widely planted and growing in Korea were evaluated by artificial inoculation of a pathogenic fungus. For this experiment, a typical virulent strain (KCPC-19) was selected. Artificial inoculation was conducted into all varieties by using two different materials and methods, i.e., bark and wood tissue sections in the laboratory and living trees in the field. In the bark and wood tissue section method, the size of necrotic area and canker development on chestnut varieties were examined and compared 4 days after inoculation. There were wide variations of chestnut varieties in disease resistance and susceptibility against chestnut blight fungus, but 3 varieties, Daebo！, Ishizuchi, and Sandae, were shown to be relatively resistant to the disease with the necrotic area of 0.95-1.03 cm2, while Arima was the most susceptible with the size of 2.0 cm2. In the living tree inoculation examined 5 weeks after inoculation, 3 varieties, Daebo, Ishizuchi, and Riheiguri, showed the higher resistance, but Tono 2 did the highest susceptibility among tested varieties.

• The Plant Pathology Journal
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• 제19권5호
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• pp.257-259
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• 2003

This report described a simple, inexpensive, faster, and effective graft inoculation method for the artificial transmission of Mungbean yellow mosaic virus (MYMV). Success of grafting and disease transmission was 100％ in this method. Screening of mungbean germplasm using this method will prevent the chance of escape infection, probably as a consequence of non-preference mechanism and loss of vector infectivity. The grafting method described here is applicable to both screenhouse and field trials.

• The Plant Pathology Journal
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• 제19권5호
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• pp.231-234
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• 2003

Brown rot symptoms severely occurred on fruits of apricot and mume grown in Changnyeong, Suwon and Yeongi in Korea during a disease survey in June 2002. A total of 32 isolates of Phomopsis sp. was obtained from the fruit rot symptoms. All isolates were identified as Phomopsis vexans based on their morphological and cultural characteristics. Four isolates of the fungus were tested for pathogenicity to fruits of apricot and mume by artificial inoculation. All isolates induced brown rot symptoms on the fruits of apricot and mume by wound inoculation. Unwounded inoculation also induced symptoms on mume fruits but not on apricot fruits. The symptoms on the fruits induced by artificial inoculation were similar to those observed in the orchard. The pathogenicity tests revealed that mume was more susceptible to the pathogen than apricot. This is the first report of brown rot of apricot and mume caused by P. vexans.

• 한국균학회지
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• 제27권6호
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• pp.378-382
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• 1999

복령의 인공재배기술을 개선하고 복령추출물의 생리활성을 검토하고자 본 연구를 수행하였다. 종균의 종류 및 접종법에 따른 검토결과, 종목 양면접종법이 균핵형성정도, 균핵 갯수, 균핵 생산수율 및 개당 평균 중량에서 가장 좋았으며, 톱밥 양면접종법이 균핵형성 및 생성수율에서 가장 낮았다 복령의 품질과 생산성을 종합하여 판단할 때, 최적 종균 및 접종방법은 종목 양면접종 > 종목 절단면접종 > 균핵 양면접종 > 톱밥 양면접종의 순이었다. 복령균핵으로부터 분리한 생리활성물질인 조 복령당의 함량은 83%이었고, 농도가 증가함에 따라 상대점도가 상승하였으며, 염의 첨가 농도에는 큰 영향을 받지 않았다. 항산화 활성(EDA, POV, TBA)을 측정한 결과, TBA가의 경우에는 천연 항산화제인 비타민 E와 유사한 활성을 나타내었으나, POV와 EDA에 의한 항산화활성 측정 결과에서는 BHT나 비타민 C보다 낮은 활성을 나타내었다.

• 한국자원식물학회지
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• 제11권
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• pp.126-128
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• 1998

• 원예과학기술지
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• 제18권6호
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• pp.797-801
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• 2000

본 연구는 고추에서 Agrobacterium tumefaciens를 이용한 형질 전환시 형질전환율을 높이기 위하여 A. tumefaciens와의 접종시간과 인위적인 상처처리에 따른 효과를 조사하였던 바 고추 형질전환시 자엽절편체의 재분화는 인위적 상처처리 및 A. tumefaciens 접종시간에 의해 영향을 받았다. 접종시간을 3600초로 한 경우의 재분화는 상처처리에 의해 급격히 감소하였으며 자엽 절편체에 대한 인위적인 상처처리가 20초 또는 120초간의 접종시간처리와 병행됨으로써 형질 전환율을 58-72% 이상으로 높이는 효과를 보였다. 형질전환여부의 확인을 ${\beta}$-glucuronidase 염색법과 npt II primer를 이용한 polymerase chain reaction 분석에 의해 수행 한 바 효과적인 것으로 판단되었다.