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Isolation and Characterization of Allelopathic Substances from Sorghum Stem (수수 줄기에 함유(含有)된 타감물질(他感物質)의 분리(分離) 및 특성(特性) 구명(究明))

  • Kim, S.Y.;De Datta, S.K.;Robles, R.P.;Kim, K.U.;Lee, S.C.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.156-162
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    • 1994
  • To better understand the exact nature of the major toxic compound responsible for phytotoxicity of sorghum stem, the most toxic compound from the stem extract was isolated by rapid chromatography and subsequently purified by thin-layer chromatography(TLC) and high pressure liquid chromatography(HPLC). Of the eight fractions isolated by rapid chromatography, the fraction with solvent combinations of butanol (8) : acetic acid (1) : water (1) had the highest toxicity. Further separation of the fraction by TLC in a solvent mixture of butanol (24) : acetic acid (16.4) : water (7) : propanol (1) showed that the spot with an $R_f$ 0.71 had one major peak with retention time of 20.40 minutes. Upon subjecting gas chromatography and the HPLC fraction to the mass spectrometry, the toxic compound is probably one of the four compounds ; 1-methyl-1-(2-propynyl)-hydrazine, 1-aziridineethanol, 5-chloro-2-pentanone, and 2-(methylseleno)-ethanamine.

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A Study of Mode of Action of Alachlor - I. Effects of Alachlor on Nucleic acid, Amino acid and Protein Synthesis in Oat(Avena sativa L.) (Alachlor의 제초기구(除草機構)에 관한 연구(硏究) - I.Alachlor가 귀리의 핵산(核酸), 아미노산 및 단백질합성(蛋白質合成)에 미치는 영향(影響))

  • Kwon, S.W.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.227-232
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    • 1990
  • The effects of alachlor [2-chloro-2', 6' diethyl-N-(methoxymethyl) acetanilide] treatment on nucleic acid, amino acid and protein synthesis were studied. The amide herbicide alachlor blocks the biosynthesis of the amino acids isoleucine, valine and aromatic amino acid in oat root tips. Nucleic acid was inhibited, but was not proportional to reduction in protein synthesis. $1{\times}10^{-4}M$ of alachlor treatment of oat roots inhibited 36% DNA synthesis, but DNA synthesis was not inhibited at $1{\times}10^{-5}M$. RNA synthesis was inhibited by $1{\times}10^{-5}M$ and $1{\times}10^{-4}M$ of alachlor 16 and 27%, respectively, while inhibition of protein synthesis did occur at same concentrations. Inhibition of protein synthesis also did not occur at concentration below $1{\times}10^{-4}M$ alachlor. It suggest that inhibition of protein sythesis caused significantly by alachlor($1{\times}l0^{-3}M$) result from secondary action.

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Late Fall Nitrogen Application and Turf Cover for Zoysiagrass (Zoysia japonica) Spring Green-up

  • Oh, Jun-Suk;Lee, Yu-Jin;Lee, Sang-Kook
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.383-389
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    • 2015
  • The use of zoysiagrass (Zoysia japonica Steud.) in the transition zone is limited because of a lack of cold hardiness although zoysiagrass has many advantages compared to other warm-season and cool-season grasses. Late-fall N fertilization is often applied for darker green color of turfgrass in early spring and more extensive root growth without rapid top growth. The objective of the study was to evaluate the effects of late fall N application and turf cover for zoysiagrass spring green-up. Clear polyvinyl chloride (PVC) film was used for turf cover. The amount of N applied were 5 and $10g\;N\;m^{-2}$ for the low and high N rate treatments, respectively. Covered zoysiagrass had greater turfgrass color and quality in early spring than non-covered zoysiagrass. The high N rate had 0.6 to 2.3 greater turfgrass quality than the low N rate on 7 of 9 rating dates. Slow-release N as late fall fertilization is more effective for turfgrass color and quality than fast-release N in spring. Turf cover could reduce the period of yellow zoysiagrass, and the earlier time of spring green-up could be advanced by increasing turfgrass quality and growth of zoysiagrass.

Weed Occurrence and Control in Simultaneous Wheat Sowing Culture with Rice Harvest under No-tilled Paddy Field (무경운(無耕耘) 벼 수확동시(收穫同時) 밀 파종에서 잡초(雜草)발생과 방제)

  • Kim, Dae-Ho;Kim, Su-Kyeong;Kim, Eun-Seok;Son, Beom-Young;Kang, Dong-Ju
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.186-190
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    • 1998
  • To establish weed control method in no-tilled and rice straw-covered wheat sown coincidentally with rice harvest using combine-attached drill seeder, several herbicides and their combination were tested for 2 years. Similar number of panicle to hand-weeding plot was secured at sprayed plot with Buta EC + Glyphosate SL mixture just after seeding. Water-foxtail(Alopeculus aequalis) and broadleaf weeds were controlled effectively by Buta EC + Glyphosate SL mixture, showing the control efficacy of 92 percent. In the 1st cropping year, weed species occurred was simple and water-foxtail predominated nearly half. However, weed species was diversified and water-foxtail predominated 83.5 percent in the 2nd cropping year. Wheat yield of Buta EC + Glyphosate SL plot showed no difference with hand-weeding plot. Dry weight of water-foxtail showed closer linear relationship with wheat yield reduction than that of broadleaf weed.

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Improvement of Herbicide Use in Crop Production IX. Tank-mix Use of Acifluorfen and Haloxyfop-methyl for Weeding on Soybeans (Glycine max L.) Cropped after Barley (제초제(除草劑)의 사용법(使用법法) 개발(開發)을 위한 연구(硏究) - 제9보(弟9報) 맥후작(麥後作) 대두(大豆)(Glycine max L.) 잡초(雜草) 방제(防除)를 위한 Acifluorfen 과 Haloxyfop - methyl 의 혼용가능성(混用可能性) 연구(硏究))

  • Guh, J.O.;Lee, K.S.;Kim, D.K.
    • Korean Journal of Weed Science
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    • v.4 no.2
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    • pp.204-210
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    • 1984
  • For weeding on the after-barley cropping soybean, the tank-mix use of postemergence herbicides acifluorfen and haloxyfop-methyl was tested. Acifluorfen has shown the transient leaf-burn on the newly formed soybean leaves, but no growth inhibitions. Under the treatment of acifluorfen only, grassy weeds occurred successively and explosively. However, under the combinated spraying of both herbicides, the grassy weeds could be controlled in a slight antagonistic, the broadleaved weeds in additive, and the total weed species including sedges in synergic tendencies, respectively. Also, to achieve the full-season weeding efficacies for the prominent crop yields, both herbicides are suggested to be mixed with more dosages than 0.245 kg ai/ha of acifluorfen and 0.15 kg ae/ha of haloxyfop-methyl, respectively.

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Soil and Morphological Characteristics of Native Zoysiagrasses by the Habitats (한국잔디류의 자생지 토양 및 생육지별 형태적 특성)

  • Lee, Sol;Yu, Han-Chun;Yoon, Byeong-Seon;Yang, Geun-Mo;Kim, Jong-Yeong;Kim, Yeong;Oh, Chan-Jin
    • Weed & Turfgrass Science
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    • v.2 no.1
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    • pp.55-61
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    • 2013
  • This study was carried out to investigate the morphological and soil characteristics of Zoysia spp. native to South Korea. Samples [41 ecotypes of Zoysia japonica, 28 ecotypes of natural hybride between Z. japonica and Z. sinica (Junggi), 22 ecotypes of Z. sinica, and 8 ecotypes of Z. matrella] were collected in seashores, levee and summit of the rock in southern Korea. Variations in leaf width, plant height, leaf angle, length of leaf sheath, leaf trichome, stolon length, and seed shape were measured at collection sites and experimental plots. Among the entries, most of the measurements did not show any significant differences between natural and experimental plot except for leaf angle of Z. sinica and the number of seeds per spike of Z. matrella, which might be caused by different environmental conditions. Soil pH was 6.0 at the most of the collection sites. $Mg^+$ was 0.06-0.02 $cmol{_c}^+kg^{-1}$, $Mg^{2+}$ was 0.09-0.03 $cmol{_c}^+kg^{-1}$, and $K^+$ was 0.02-0.007 $cmol{_c}^+kg^{-1}$ at most zoysiagrasses growing soils.

Influence of Light, Osmotic Potential, pH and Rice Straw Mulching on Growth of Barley(Hordeum vulgare L.) and Water Foxtail(Alopecurus aequalis var. amurensis(Kom.) Ohwi) (보리와 독새풀의 생장에 대한 삼투압, pH 및 볏짚멀칭의 영향)

  • Lee, Choon-Woo;Kim, Yong-Wook;Koo, Bon-Cheol
    • Korean Journal of Weed Science
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    • v.17 no.3
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    • pp.310-313
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    • 1997
  • The seedling of barley and weed would be generally inhibited by rice straw residues in the field. This experiment was carried out to investigate the allelopathic effects of rice straw and environmental conditions of germination such as osmotic potential, pH on the seedling of barley and water foxtail. The pH did not affect the germination of barley and water foxtail. Osmotic potential to -2.9bar, -0.6bar did not affect the germination of barley and water foxtail, respectively. Rice straw mixed with soil at the concentration of 4%(w/w) reduced the dry weight of shoot and root, and volume of barley root, as compared to the untreated control. Dry weight of water foxtail by rice straw mulching was decreased by 87.1%, 86.2%, respectively.

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Response of Systemic Fungicides of Rhizoctonia spp. Causing Rhizoctonia Blight on Turfgrass (잔디에 Rhizoctonia 마름병을 유발하는 Rhizoctonia spp.의 침투성 살균제에 대한 반응)

  • Chang, Taehyun;Lee, Seong Jun
    • Weed & Turfgrass Science
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    • v.2 no.4
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    • pp.387-394
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    • 2013
  • The Rhizoctonia blight causing by Rhizoctonia spp. is an important disease of turfgrass, requiring fungicide application to maintain acceptable conditions for turfgrass good qualities in the golf course. The experiment was conducted to determine the mean 50% effective concentration inhibiting mycelial growth ($EC_{50}$) value of to flutolanil, pyraclostrobine and hexaconazole to Rhizoctonia solani AG-1 IB, Rhizoctonia cerealis and Rhizoctonia solani AG2-2IV isolated from Gyeongbuk province of Korea in vitro. Five discriminatory concentrations of each fungicide were used to detect in vitro sensitivity. The mean of $EC_{50}$ values to three systemic fungicides was the lowest isolate of R. solani AG-1 IB. However, the sensitivity of fungicides to Rhizoctonia solani AG2-2IV were higher mean $EC_{50}$ value of 0.026 ${\mu}g\;a.i.\;ml^{-1}$ of pyraclostrobine and 0.044 ${\mu}g\;a.i.\;ml^{-1}$ of flutolanil. R. cerealis was the lowest sensitivity to hexaconazole which was an average $EC_{50}$ value of 0.022 ${\mu}g\;a.i.\;ml^{-1}$. Inhibition of mycelial growth rate (%) by three combine fungicides using the $EC_{50}$ value of each fungicide was the highest R. solani AG2-2IV. Results of this study were may confirmed in vitro response fungicide of three Rhizoctonia species for control of Rhizoctonia blight in the field.

A Study of Mode of Action of Alachlor - II. Effect of Alachlor on Peroxidase Synthesis in Oat(Avena sativa L.) (Alachlor의 제초기구(除草機構)에 관한 연구(硏究) - II. Alachlor가 귀리의 Peroxidase합성(合成)에 미치는 영향(影響))

  • Kwon, S.W.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.233-239
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    • 1990
  • The effect of alachlor treatment on peroxidase synthesis in oat root tips was studied. Alachlor caused increase in the amount of soluble peroxidase in oat root tips, peroxidase activity increase as the rate of alachlor application increased, Alachlor treatment of oats with $1{\times}10^{-6}M$, peroxidase activity increased 0.20 unit higher than that of nontreatment. After 12hr, 65mM of peroxide treatment of oats inhibited 16% root growth, and 130 mM peroxide treatment caused 59% inhibition. With PAGE of peroxidase extracted from normal root tips, PAGE give 4 species($P_2$, $P_3$, $P_4$, and $P_5$ band) of peroxidase. Alachlor significantly activated isoperoxidase. Three isoperoxidase($P_1$, $P_6$, and $P_7$) are synthesized at a increased concentration of alachlor, SDS-PAGE analysis of proteins extracted from oat root tips showed that they were made up of subunits blow 100 kD polypeptide.

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Moisture Sorption Isotherms of Four Echinochloa Species Seeds (피속 잡초 종자의 등온흡습곡선 특성)

  • Lee, Yong Ho;Byun, Ji Young;Na, Chae Sun;Kim, Tae Wan;Kim, Jeong-Gyu;Hong, Sun Hee
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.111-117
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    • 2015
  • The equilibrium moisture contents (EMC) in seeds of four Echinochloa (E. crus-galli var. crus-galli, E. crus-galli var, echinata, E. crus-galli var. praticola, E. esculenta) were measured at $20^{\circ}C$ with equilibration over a series of lithium chloride solutions with relative humidities ranging from 0.11 to 0.8 to determine sorption isotherms and safe storage relative humidity. Standard seed sorption isotherm models i.e. modified Henderson, modified Chung-Pfost, modified Halsey, modified Oswin and Guggenheim-Anderson-deBoer (GAB) equations were adopted to evaluate the goodness of fit to sorption isotherms. This study indicated that EMC of seeds was significantly different in four Echinochloa species at various relative humidity. The modified Oswin equations for E. crus-galli var. crou-galli, E. crus-galli var, echinata, E. esculenta and GAB equation for E. crus-galli var. praticola were adequate models for the EMC data. Seeds of four Echinochloa species have monolayer moisture contents when stored at RH < 0.1. These results show that seed moisture isotherm model should be selected according to genetic variation.