• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.351-351
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• 2017

Arbuscular mycorrhizal fungi (AMF) are particular soil fungi that benefit many crops and require a symbiosis with plant roots to survive. In our previous study, there was a positive correlation between AMF root colonization and soybean grain yield in a four-year consecutive winter cover crop-soybean rotational system without phosphorus fertilizer. It is suggested that higher AMF root colonization can be a better solution for improving soybean growth and grain yield in P-limited soil. Our purpose in this study was to test the hypothesis that a P application is the main factor improving soybean growth, P nutrition and grain yield, and the benefit from AMF to soybean P uptake and growth in a P-limited soil. Impact of a P application on AMF root colonization and communities in soybean roots and their potential contribution to soybean growth and P nutrition under a five-year P-unfertilized crop rotational system were investigated over two-years. In this study, four cover crop treatments included 1) wheat (Triticum aestivum); 2) red clover (Trifolium pratense); 3) rapeseed (Brassica napus); and 4) fallow in the crop rotation. The amount of triple superphosphate as a P fertilizer applied rate after cultivation of cover crops was 120 and $360k\;ha^{-1}$ in 2014 and 2015, respectively. Soybean roots were sampled at full-flowering and analyzed for AMF communities using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative real-time PCR (qPCR) techniques. The AMF root colonization in the soybean roots at full bloom stage was significantly influenced by cover crop and P application throughout the two-year rotation. The two-year rotation of different cover crops or fallow impacted the molecular diversity of AMF communities colonizing roots of soybean. Redundancy analysis (RDA) indicated that AMF communities colonizing roots of soybean were significantly different among cover crop rotations. The AMF communities colonizing roots of soybean were clearly influenced by a P application in the two-year trial. Moreover, a P application may have positively impacts on the AMF communities under P-deficit soil due to the continuous cover crop-soybean rotational system without a P fertilizer.

• 한국토양비료학회지
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• 제48권2호
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• pp.81-86
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• 2015

Soil management for orchard depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 orchard (5 sites for sandy loam, 7 sites for silt loam, and 13 sites for loam) in Gyeongnam Province by fatty acid methyl ester (FAME) method. The average values for 25 orchard soil samples were $270nmol\;g^{-1}$ of total FAMEs, $72nmol\;g^{-1}$ of total bacteria, $34nmol\;g^{-1}$ of Gram-negative bacteria, $34nmol\;g^{-1}$ of Gram-positive bacteria, $6nmol\;g^{-1}$ of actinomycetes, $49nmol\;g^{-1}$ of fungi, and $7nmol\;g^{-1}$ of arbuscular mycorrhizal fungi. In addition, silt loam soils had significantly low ratio of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ compared with those of loam soils (p < 0.05), indicating that microbial activity increased. The average soil microbial communities in the orchard soils were 26.7% of bacteria, 17.9% of fungi, 12.6% of Gram-negative bacteria, 12.5% of Gram-positive bacteria, 2.5% of arbuscular mycorrhizal fungi, and 2.2% of actinomycetes. The soil microbial community of Gram-negative bacteria in silt loam soils was significantly higher than those of sandy loam and loam soils (p < 0.05).

• 한국토양비료학회지
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• 제46권5호
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• pp.373-378
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• 2013

Information on the effective application method of arbuscular mycorrhizal fungi (AMF) inoculum is still inadequate. This work was performed to assess two AMF inoculation methods (dispersed and proximate) on root colonization of sorghum-sudangrass hybrid (Sorghum bicolor L.). In dispersed inoculation method, spores were inoculated in 2 kg pots of soil in which 5 day-old seedlings were transplanted and maintained for 50 days. In the proximate inoculation method, spores were first introduced in 500 mL pots where seeds were sown. After 10 days, the seedlings with the 500 mL soil were transferred to 2 kg pots without disturbing the contents. After 50 days of growth, root colonization and arbuscule abundance significantly increased (over 100%) in proximate method of inoculation. Moreover, sorghum-sudangrass hybrid had higher shoot growth (182.5 cm) and Glomalin related soil protein (GRSP) production in proximate method. Nutrient accumulation, particularly total nitrogen (82.61 mg $plant^{-1}$), was also found to be higher in proximate method of inoculation. Our results demonstrate that the proximate method of inoculation may improve the early stage mycorrhizal symbiosis and inoculum performance in Saemangeum reclaimed soil.

• 한국토양비료학회지
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• 제36권3호
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• pp.145-153
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• 2003

조사지역의 평균 토양화학성은 pH 5.93, 유기물함량 $25.9g\;kg^{-1}$, 유효인산 $742mg\;kg^{-1}$, $NO_3-N$ $44.7mg\;kg^{-1}$ 이었으며, 토양 1 g당 균근균 포자는 양파 12.1, 마늘 11.7, 고추 10.1로 작물별 큰 차이를 보이지 않았다. 토성에 따른 포자수는 식토와 미사질 식토에서 토양 1 g 당 15개 이상의 포자밀도를 보였으며, 토양 pH, OM, $NO_3-N$, Av. $P_2O_5$등 토양양분함량과 포자밀도는 유의성 있는 상관관계를 보이지 않았으나, 포자수는 양분농도와 관계없이 일정한 수준을 보였다. 종이 확인된 균근균 수집 포자는 Glomus속 2종과 Gigaspora속 3종으로 Glomus clarum, Glomus intraradices, Glomus etunicatum와 Gigaspora rosea, Gigaspora margarita 등 이었다.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.147-154
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• 2005

A survey for vesicular-arbuscular mycorrhizae (VAM) status was undertaken in three different industrially polluted sites at Uyyakondan channel of Senthanneerpuram area in Trichy, India. The soils and the effluents were acidic, and contained higher Zn (621 to 711 ppm) than the other heavy metals, such as Cu, Pb, and Ni. Eighteen plant species were collected from the rhizosphere soils, and 13 species were positive for VAM colonization. Fifteen VAM fungal species were isolated from the plant species. The number of VAM fungal spores from the soils ranged from 45 to 640 per 100 g of soil. There was a significant correlation observed between the number of spores and percentage root colonization, as exemplified by Acalypha indica (45 and 20%, respectively) and Paspalum vaginatum (640 and 98%, respectively). Hostspecific and site-specific associations were observed in site 2; particular VAM species, Gigaspora gigantea and Glomus fasciculatum, were specific to particular host plants, Phyllanthus maderaspatensis and A. indica, respectively, even though Eclipta prostrata and Physalis minima were maximally associated with 8 VAM species. G. fasciculatum was found in 11 plant species and predominant VAM species. These results led us to conclude that VAM fungi are associated with a majority of the plants in the industrial polluted sites and support the plants to survive in the acidic soils, polluted with heavy metals of the industrial effluents.

• 한국토양비료학회지
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• 제43권4호
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• pp.498-505
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• 2010

AMF 접종원 처리가 원예작물의 생육에 미치는 효과를 구명하기 위하여 상추 (Lactuca sativa L.)에 파종단계와 이식단계에서 수단그라스를 기주식물로 증식한 g 당 30개의 포자와 AMF가 감염된 뿌리, 균사 및 토양이 혼합된 접종원을 처리한 후 이식 전후에 걸쳐 작물의 생장반응과 수량성을 비교 검토하였으며, AMF 포자밀도와 감염률 및 감염양상을 조사하였고, 작물의 무기양분의 흡수양태를 분석하였다. 상추의 파종단계에서 AMF 접종원을 처리한 후 생육반응을 조사한 결과, 파종 후 3주가 경과한 초기에서 AMF 접종원 처리구의 상추는 대조구보다 엽수는 9%, 엽의 생체중은 59%, 엽면적은 58% 및 엽장과 엽폭은 21-22%가 증가하였으며 엽록소함량은 2%가 향상되었다. 파종 후 9주가 경과하여서는 파종단계와 이식단계에서의 AMF 접종원 처리구는 대조구에 비해 엽수는 21%와 18%, 엽의 생체중은 51%와 41%, 근체중은 56%와 47%가 각각 증가하였으며 엽록소함량은 18%와 19%가 향상된 것으로 조사되었다. 상추 엽의 P 함량을 분석한 결과 이식단계에서 AMF 접종원 처리구가 대조구보다 217%가 증가된 P 함량을 보임으로서 P의 흡수이용이 증진되었고 상추의 생육에 영향을 미친 것으로 판단된다. 상추 근권토양의 AMF 포자밀도는 상추의 생육초기에 AMF 접종원 처리구가 대조구의 3배를 상회하는 높은 수준으로 나타났고, 9주가 경과하였을 때는 276-295%가 증가된 것으로 조사되었다. AMF 감염률 또한 대조구에 비해 크게 향상된 것으로 조사되었고 생육 후기에는 낭상체와 균사가 치밀하게 발달됨으로서 AMF 의존도가 높음을 확인하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 추계학술발표회 요지
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• pp.174-175
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• 2005

• Journal of Ginseng Research
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• 제19권1호
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• pp.73-76
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• 1995

Newly sprouted American ginseng (Panax quinquefolium L.) seedlings were transplanted to forest pots with mycorrhizae-infested soil and grown in screenhouse for 2 years. Growth patterns, mortality rate and fresh root weight were investigated. Plants in VAM soil had lower mortality rales than control. In soils infested with two species of mycorrhizal fungi (Glomu deseyicola, frappe, Bloss and Merge and G. intraradices, Schenck and Smith), 28-35% of plants produced 3-prongs in the second season and significantly increased fresh root weight by 41 to 43%.

• Mycobiology
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• 제51권3호
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• pp.164-168
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• 2023

This study examined the effects of myristate on an asymbiotic culture of Rhizoglomus intraradices, a species of arbuscular mycorrhizal fungi (AMF; Glomeromycota). Mycelial growth and sporulation in a modified medium containing myristate were observed. The findings demonstrated that myristate induced R. intraradices spore formation, with daughter spores having a smaller diameter than the parent spores. This observation is consistent with previous studies on other Rhizoglomus species. Further studies are needed to investigate the potential for continuous culture, mass production using daughter spores, and the application of AMF colonization techniques in plants.

• 한국토양비료학회지
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• 제40권6호
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• pp.476-481
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• 2007

친환경 재배지 토양과 관행토양간 토양화학성은 큰 차이를 보이지 않았으며, 토양 유효인산 함량은 친환경 재배지 토양이 관행 재배지 토양과 비교하여 다소 높은 수준이었다. 토양 관리방법에 따른 균근균 포자밀도는 친환경 재배지 토양이 건토 30 g 당 140여개의 포자밀도를 나타낸 반면 관행 재배지 토양은 60여개 내외의 포자밀도를 보였다. 각 토양 화학성과의 상관관계를 비교한 결과, pH, EC, OM, $Av.P_2O_5$, K Ca+Mg), CEC에 대한 관행재배지 토양의 균근균 포자밀도는 각각 $-0.48^*$, -0.05, $0.48^*$, -0.12, -0.13, 0.31의 상관계수를 나타냈다. 반면, 친환경 재배지 토양에 대한 균근균 포자밀도는 각각 $-0.68^*$, $0.69^*$, $0.96^{**}$, $0.75^*$, $0.63^*$, $0.92^{**}$의 상관계수를 보여 모든 무기성분이 유의성을 보였다. 뿌리의 균 조직은 주로 내부균사가 많았으며, 수지상체와 낭상체는 매우 낮은 수준이었다. 지역별로는 친환경 재배관리를 한 순천과 천안에서 다소 높은 수준의 감염정도를 나타냈다.