• 한국미생물·생명공학회지
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• 제30권3호
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• pp.270-281
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• 2002

장내 유용세균인 L. acidophilur및 L. plantarum은 선택되어진 천연산물의 한 종류 단독으로서도 촉진되었으며, 또한 두 종류, 세 종류 및 네 종류의 배합에 의하여 특징적으로 성장이 촉진되었으며, L. acidophii요는 이들 천연산물의 배합 중에서 한 종류의 천연산물로서는 백작약, 석창포, 진피 등이 좋은 효과를 보였고, 두 종류 천연산물의 배합에서는 석창포와 계피, 진피와 갈근 등의 조합이, 세 종류의 천연산물 배합에서는 승마와 계피 및 감초 등의 조합이, 네 종류의 천연산물 배합에서는 감초, 녹차, 황금 및 계피 등의 조합에서 잘 성장함을 보였으며, 네 종류의 조합에 의한 세균의 성장 촉진 효과는 대조군보다는 1.3배 이상의 효과를 보였으며 이때의 항산화력은 대조 군보다도 4배 이상의 강함을 보였고, hydroxyl radical의 소거활성은 천연산물을 100 배 희석하였을 때 70% 이상의 소거능력을 보였다. 또한,1. plantaurm은 한 종류의 천연산물로서는 산수유, 연자육, 괴화 등이 좋은 효과를 보였고, 두 종류의 천연산물의 배합에서는 오미자와 녹차, 백작약과 음양곽, 현초와 녹차 등의 조합이, 세 종류의 천연산물 배합에서는 산수유와 녹차 및 백작약, 산수유와 황련 및 오미자, 산수유와 오미자 및 백작약 등의 조합이,4종류의 천연산물 배합에서는 산수유, 녹차, 황련 및 황금의 조합, 산수유, 오미자, 백작약 및 녹차의 조합, 산수유, 녹차, 황련 및 현초 등의 조합에서 L. plantarum가 잘 성장함을 보였고, 이 례 종류의 조합에 의한 세균의 성장 촉진 효과는 대조군보다 1.5배 이상의 효과를 보였고, 항산화력은 수-5배 이상으로 나타났으며, hydroxyl radical의 소거활성은 천연산물을 100배 희석하였을 때 75% 이상의 소거능력을 보였다. 이러한 천연산물의 배합으로 생체의 특정부위를 활성화시킬 수 있고, 항산화력으로 유해 산소를 제거 할 수 있으며, 천연산물 고유의 약성을 활용할 수 있고, 여러 가지의 유용세균의 성장 촉진 효과를 기대할 수 있어 생체에 긍정적인 효과를 제공할 수 있을 것으로 사료되며, 기능성 항산화 차등의 건강 식품의 원료로 사용할 수 있을 것으로 판단된다.

• Toxicological Research
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• 제25권4호
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• pp.159-173
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• 2009

Nuclear factor erythroid derived 2-related factor-2 (Nrf2) is a master transcription regulator of antioxidant and cytoprotective proteins that mediate cellular defense against oxidative and inflammatory stresses. Disruption of cellular stress response by Nrf2 deficiency causes enhanced susceptibility to infection and related inflammatory diseases as a consequence of exacerbated immune-mediated hypersensitivity and autoimmunity. The cellular defense capacity potentiated by Nrf2 activation appears to balance the population of $CD4^+$ and $CD8^+$ of lymph node cells for proper innate immune responses. Nrf2 can negatively regulate the activation of pro-inflammatory signaling molecules such as p38 MAPK, NF-${\kappa}B$, and AP-1. Nrf2 subsequently functions to inhibit the production of pro-inflammatory mediators including cytokines, chemokines, cell adhesion molecules, matrix metalloproteinases, COX-2 and iNOS. Although not clearly elucidated, the antioxidative function of genes targeted by Nrf2 may cooperatively regulate the innate immune response and also repress the expression of pro-inflammatory mediators.

• Food Science and Biotechnology
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• 제18권4호
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• pp.1051-1054
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• 2009

This study was conducted to investigate the antioxidant activity of the extracts of lotus (Nelumbo nucifera Gaertner). The total phenolic contents in leaf, stem, and root were 165, 74, and 30 tannic acid equivalent mg/g of dried extract or fraction respectively. The butanol and ethylacetate fractions of lotus parts showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than other fractions. 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity also showed the similar result as the DPPH radical scavenging activity. The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.91-92
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• 2003

Oxidative stress induced by reactive oxygen intermediates (ROIs) has been implicated in a variety of human diseases including cancer, diabetes, rheumatoid arthritis and neurodegenerative disorders. Hydrogen peroxide ($H_2O$$_2$), a representative ROI which is produced during the cellular redox process, can cause cell death via apoptosis and/or necrosis depending on its concentrations. (omitted)

• Journal of Applied Biological Chemistry
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• 제44권2호
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• pp.71-76
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• 2001

Methanol extract and solvent fractions of Aster scaber Thunb. were tested to elucidate antioxidative characteristics in vitro. Methanol extract showed strong inhibition on linoleic acid autoxidation and conjugated diene production. Hexane and diethyl ether fractions showed stronger chelating effect on $10^{-4}M\;FeCl_3$ than the other fractions. Butanol fraction had the strongest capacity on $10^{-3}M\;CuSO_4$ similar to ${\alpha}$-tocopherol. Synergistic effect of butanol fraction with histidine, methionine, and lysine on linoleic acid autoxidation had been observed. Contents of total phenol, vitamin E, vitamin C, and carotenoid were evaluated. From the results, Aster scaber Thunb. could be expected to play a role as an antioxidant in vivo.

• 생약학회지
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• 제46권4호
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• pp.303-308
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• 2015

Ten phenolic compounds were isolated from the twigs of Stewartia pseudocamellia. The isolated compounds were identified as 5,7,3',5'-tetrahydroxyflavanone (1), 3,5,7,3',5'-pentahydroxyflavanone (2), quercetin (3), (+)-aromadendrin (4), (+)-ampelopsin (5), myricetin (6), (+)-catechin (7), (-)-epicatechin (8), kaempferol (9), and fraxin (10) by spectroscopic analysis. Compounds 1, 2, 4, 6, 8, and 9 were isolated from this plant for the first time. The antioxidant activities of compounds 1-10 were evaluated by the DPPH and/or ORAC (oxygen radical absorbance capacity) assay. Compounds 3, 5-9 showed significant antioxidative effects on DPPH assay. Among the active compounds, 6 exhibited higher activity compared to trolox on ORAC assay.

• 생명과학회지
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• 제7권4호
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• pp.289-296
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• 1997

Antioxidative activity of fraction extracted from cultivation of Aspergillus sp. A-5 against oxidation of human low density lipoprotein(LDL) was investigated. Fractions of Aspergillus sp. A-5 cultivation was sucessively purified with ethyl acetate and silica gel column chromatography. The concentration of fraction 4 inhibited Cu$^{2+}$-induced oxidation of LDL almost completely. Band 3 isolated by the further purification of fraction 4 was higher than that of same concentration of $\alpha$-tocopherol, BHA and BHT. The elcetrophoretic mobility of oxidized LDL by addition of Band 3 was faster than that of native LDL, but slower than that of oxidzed LDL. It is concluded that fraction of Aspergillus cultivation contained antioxidants with the capacity to inhibit oxidative modification of LDL.

• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.298-304
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• 2006

This study was carried out to elucidate the relationship among exercise, bone mineral density and antioxidant enzyme activity of postmenopausal women. 60 women residing in the Iksan, Korea area were recruited. The questionnaires were designed to find out exercise habits. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. Parameters of antioxidative capacity, including the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TA) were analyzed in fasting blood. The mean age, height, weight, and BMI of subjects were 65.0 years, 151.1 cm, 59.5 kg $26.0\;m/kg^2$, respectively. The mean BMDs of subjects were $0.85\;g/cm^2$ (lumbar spine), $0.6\;g/cm^2$ (Femoral neck), $0.49\;g/cm^2$ (trochanter), and $0.40\;g/cm^2$ (Ward's triangle). There was a significant difference in BMD among different age groups (50's, 60's and 70's) showing lower value with increasing age (p<0.05). The mean SOD, GPx, and CAT activities were 138.5 U/mL, 1,273.8 U/mL and 314.3 kU/L respectively, and TA was 1.16 mmol/L. TA of the group which exercised 3$\sim$4 times a week was significantly higher than those of the other exercise groups (p<0.05). The subjects with higher SOD activity also have a higher the T values in the lumbar spine, femoral neck, trochanter, and Ward's triangle. In conclusion, this study revealed that the levels of antioxidant enzyme activity were closely associated with the exercise status and bone mineral density in postmenopausal women.

• 한국자원식물학회지
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• 제23권5호
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• pp.406-414
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• 2010

본 연구는 제주도에 자생하는 차나무과 식물을 대상으로 식품소재 또는 생약으로의 활용 방안을 모색하고자 angiotensin I converting enzyme(ACE) 저해활성, aminopeptidase N(APN) 저해활성 및 $\alpha$-amylase 저해활성을 조사하고, 항산화활성을 검색하고 TLC를 이용하여 분석하였다. ACE 저해활성은 후피향나무(수피)와 비쭈기나무(잎)에서 50% 이상의 저해활성을 보였으며, APN 저해활성은 비쭈기나무(잎과 수피)와 후피향나무(수피)에서만 양의 활성을 보였다. $\alpha$-amylase 저해활성은 동백나무(열매), 우묵사스레피나무(수피), 후피향나무(수피)와 차나무(줄기)에서 30% 이상의 저해활성을 보였다. 항산화활성은 비쭈기나무(수피), 후피향나무(수피), 차나무(잎)에서 30% 이상의 다소 높은 전자공여능을 나타내었다. 특히, 비쭈기나무(수피)는 dot-blot test에 의해 다른 종에 비해 활성이 높아 $1.25\;{\mu}g/ml$의 낮은 농도에서도 높은 항산화활성을 보였다. TLC 분석에 의해 비쭈기나무(수피)에서 EGC(Rf 0.26) 활성이 높았으며, 비쭈기나무, 우묵사스레피나무, 후피향나무의 수피에서 EGCG(Rf 0.09) 활성이 높게 검출되었다. 그리고, 표준 catechin류와는 다른 것으로 보이는 5개의 밴드(Rf 0.54, 0.46, 0.44, 0.16, 0.03)는 Folin-Ciocalteu Reagent 방법과 Ferric chloride-alcohol 방법을 이용하여 polyphenol류인 것으로 추정되었다. 이상의 결과를 토대로 사스레피나무를 제외한 차나무과 식물들은 생리활성이 높아 식품 소재 또는 생약으로의 활용이 가능할 것으로 보이며, 활성성분의 분리 및 동정 그리고 이들 물질을 이용한 임상실험 등 보다 심도있는 연구가 필요할 것으로 보인다.

• Nutritional Sciences
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• 제6권3호
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• pp.141-147
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• 2003

Fruits and vegetables reportedly have a protective effect against hyperlipidemia and oxidative disease. Accordingly, this study aimed to investigate the lipid-lowering effect and antioxidative capacity of persimmon leaf extract (PLE) in rats fed a high-cholesterol diet. Male rats were fed a high-cholesterol (1% wt/wt) or high-cholesterol diet supplemented with Lovastatin (0.02% wt/wt) or PLE (0.2% wt/wt) for 5 weeks. The concentration of plasma total cholesterol was significantly lower in the PLE group than in the lovastatin group. However, the concentration of plasma HDL-cholesterol and the ratio of HDL-cholesterol/total-cholesterol (%) were significantly higher in the PLE group than in the control group. The PLE supplement also significantly lowered the contents of hepatic cholesterol and triglyceride. In comparing fecal sterol contents, the PLE group saw a significant increase of both neutral and acidic sterol compared to the other groups. The PLE supplement significantly lowered plasma GOT and GPT activity, which ave indices of hepatic toxicity. Plasma TBARS concentration was significantly lower in the PLE group than in the control group, while hepatic TBARS level was not significantly different between the groups. In a comparison of hepatic antioxidant parameters, SOD, catalase and GSH-Px activity were significantly higher in the PLE group than in the control group. However, the PLE supplement significantly towered antioxidant enzyme activity in the erythrocyte. Furthermore, these results suggest that supplementation of PLE promoted the excretion of fecal sterols, thereby leading to decreased absorption of dietary cholesterol. In addition, PLE may play an important role in regulating antioxidative capacities by altering SOD and ChT activity.