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Antioxidative activity, including Inhibitory activities of ACE, APN and $\alpha$-amylase, in Theaceae Plants Native to Jeju Island  

Oh, Soon-Ja (Department of Biology & Research Institute for Basic Sciences, Jeju National University)
Lee, Jin-Ho (Sulloc Cha Institute of Jangwon Co. Ltd.)
Ko, Kwang-Sup (Sulloc Cha Institute of Jangwon Co. Ltd.)
Shin, Dong-Bum (Department of Food Science & Nutrition, Jeju National University)
Koh, Seok-Chan (Department of Biology & Research Institute for Basic Sciences, Jeju National University)
Publication Information
Korean Journal of Plant Resources / v.23, no.5, 2010 , pp. 406-414 More about this Journal
Abstract
Antioxidative activity, including inhibitory activities of angiotensin I converting enzyme(ACE), aminopeptidase N(APN) and $\alpha$-amylase, was investigated in the methanol extracts from Theaceae plants native to Jeju island, in order to select the plant species containing bioactive materials for functional food or medicines. ACE inhibitory activity was above 50% in Ternstroemia japonica(stem bark) and Cleyera japonica(leaf), and APN inhibitory activity was low to be positive only in C. japonica(leaf, stem bark) and T. japonica(stem bark). $\alpha$-Amylase inhibitory activity was above 30% in Camellia japonica(fruit), Eurya emarginata(stem), T. japonica(stem bark) and Thea sinensis(stem). The antioxidative activity, estimated by the DPPH radical scavenging capacity, was above 30% in C. japonica(stem bark), T. japonica(stem bark) and T. sinensis(leaf). Particularly, the antioxidative activity analyzed by dot-blot test was very high in C. japonica(stem bark) relatively to those of other plants, and remained high in the low concentration($1.25\;{\mu}g/m{\ell}$). From the TLC analysis of antioxidative compounds, EGC(Rf 0.26) was found to have high activity in stem bark of C. japonica and EGCG(Rf 0.09) was found to have high activity in stem bark of C. japonica, E. emarginata, and T. japonica. Five bands (Rf 0.54, 0.46,0.44, 0.16, 0.03) which were not identified as compared with catechins were detected as polyphenolic compounds on the TLC plates sprayed with the Folin-Ciocalteu solution or the Ferric chloride-alcohol solution. These results suggests that Theaceae plants except E. japonica could be potentially used as a resource of bioactive materials for functional foods or medicines and further research is reguired to identify the bioactive substances and determine the functions of them.
Keywords
Theaceae plants; inhibitory activity; angiotensin I converting enzyme(ACE); aminopeptidase N(APN); $\alpha$-amylase; antioxidative activity; bioactive materials;
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