• 약학회지
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• 제44권3호
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• pp.257-262
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• 2000

The roots of Cynanhum caudatum (Asclepiadaceae) have been used in folk medicine in Japan and China for the prevention and treatment of various geriatric diseases and also as a cardiotonic agent. Constituents of this plant have mainly been examined for glycosides: besides two steroidal alkaloids, gagaminine and gagamine which was firstly isolated by us, more than 35 polyoxypregnane glycosides and aglycones have also been identified. Gagaminine inhibits potently the hepatic aldehyde oxidase activity and lipid peroxidation in vitro. The present work deals with the comparison of antioxidative activities of gagamine, a new pregnane alkaloid, three isolated polyoxypregnanes containing a keto group at C-20 with those of gagaminine, a potent antioxidant, in order to explain the structure-activity relationships. The results of this study further prove that the cinnamoyl group of gagaminine is very important for the inhibition on the aldehyde oxidase activity while the nicotinoyl group is necessary for anti-lipid peroxidation. Besides that, the keto compounds having no ester group at C-12 were found to be more active than the others except gagaminine.

• Journal of Pharmaceutical Investigation
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• 제28권4호
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• pp.241-247
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• 1998

Ketoconazole is an imidazole antifungal agent which inhibits the biosynthesis of fungal cellmembrane ergosterol and has immunosuppressive properties in vitro. Biphenyl dimethyl dicarboxylate (PMC) has been utilized for antioxidative action and for liver-protective purposes. Studies were undertaken to investigate effects of biphenyl dimethyl dicarboxylate (PMC) on the immunosuppression of ketoconazole in ICR mice. In the combination of PMC and ketoconazole, as compared with the treatment of ketoconazole alone, there were significant increases in activities of natural killer (NK) cells and phagocytes along with circulation leukocytes. The elevation of serum glutamic-pyruvic transaminase (S-GPT) and total protein levels caused by ketoconazole were reduced by the combination of PMC and ketoconazole. In addition, lower serum albumin and albumin/globulin (A/G) ratio were also increased to normal level.

• 한국응용과학기술학회지
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• 제28권2호
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• pp.219-224
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• 2011

In this study, we evaluated the anti-oxidative, whitening and anti-wrinkle effect of Tetrapanax papyriferum extract. Tetrapanax papyriferum was extracted by two different solvents which were n-Hexane, ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase, elastase for anti-wrinkle were investigated. For anti-oxidant activity, ethyl acetate fraction of Tetrapanax papyriferum extract showed more significant activity than n-Hexane fraction of Tetrapanax papyriferum extract. For whitening activity, n-Hexane fraction of Tetrapanax papyriferum extract exhibited strong inhibition effects compared with reference. Therefore, Tetrapanax papyriferum extract may be useful as a new antioxidant and whitening agent.

• 약학회지
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• 제47권2호
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• pp.93-97
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• 2003

Kombucha fermentation broth has been used as a popular health beverage and an alternative therapy with prophylactic and therapeutic benefit. We tried to establish optimal culture conditions for Kombucha fermentation in milk and to investigate cytotoxicity and antioxidative enzyme activity of Kombucha broth against normal human fibroblasts. The optimal conditions of Kombuch culture were established to 3$0^{\circ}C$, 20∼23 hours by DPPH radical scavenging test. There were positive effects on cell growth while no cytotoxicity against primary normal human diploid fibroblasts was found. The activites of glutathione peroxide and catalase in the cells treated by hydrogen peroxide (1 mM) alone and by hydrogen peroxide with Kombucha broth (1 mg/mι) were significantly different (p＜0.05). These results suggest that Kombucha broth could be developed as an antioxidant agent for a new cosmetic material.

• KSBB Journal
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• 제18권4호
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• pp.324-328
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• 2003

도꼬마리 추출물의 발암성의 유무를 검토하기 위하여 시험균 S. typhimurium TA98 및 TA100에 대한 변이원성 실험 결과, 추출물 XE-N과 XEA-N의 농도가 증가해도 TA 98 및 TA100 두 균주의 복귀변이 colony 수가 대조구에 비해 변화가 크지 않은 점을 미루어 보아 각 추출물은 변이원성이 없는 것으로 확인되었다. 도꼬마리 추출물 XEA-A, XEA-B 및 XE-A)의 $H_2O$$_2$로 유도된 세포 독성에 대한 억제효과는 각각 58%, 53%, 63%로서 생존율이 2배 이상 증가하였다. 면역 증강 활성은 TNF의 경우 XE-N 추출물에서 2.7배로 가장 높은 활성을 나타내었으며 XEA-N 추출물에서도 2.3배로 높은 활성을 나타내었다. IL-1$\beta$의 생성은 XE-B 추출물에서 높게 나타났으며, IL-2의 생성에는 뚜렷한 활성을 나타내지 않았다. 도꼬마리 추출물 및 정제물을 이용하여 동물에 강력한 발암물질인 MNNG를 투여한 후 항산화 효소계를 측정한 결과, 조직의 해독물질인 glutathione (GSH)의 함량이 도꼬마리 정제물 0.2% 투여군에서 약 44.6%, 도꼬마리 추출물 투여군에서 약 41.5% 증가되었다. 또한 도꼬마리 추출물 및 정제물질은 암억제 유전자인 p53의 발현을 매우 증가시켰다. 이상의 결과에서 도꼬마리 추출물은 전반적으로 항산화능, 면역증강효과, 암 예방효과 등의 기능성이 있는 것으로 입증되었으며, 각 기능에 따른 다양한 물질을 분리하여 신소재로의 개발 가능성이 큰 약제임이 확인되었다.

• 대한본초학회지
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• 제30권6호
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• pp.17-24
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• 2015

Objectives : Persicaria tinctoria belongs to the Polygonaceae family and it has been used as the natural dye traditionally. Also, it is well known that the Persicaria tinctoria is used for treating the following symptoms such as fever, inflammation and edema. The purpose of this study is to investigate the effective source of antioxidants and anti-inflammatory agent from various parts of Persicaria tinctoria.Methods : We investigated the antioxidative and anti-inflammatory properties of the Persicaria tinctoria extracts. Antioxidant activities were measured by 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, Fe²⁺ chelating activity and Reducing power of Persicaria tinctoria extracts. And its inhibitory effect against oxidative DNA damage was evaluated in non-cellular system using φX-174 RF I plasmin DNA. The anti-inflammatory effect of Persicaria tinctoria was measured by using the inhibitory efficacy for the amount of nitric-oxide (NO) produced in LPS induced RAW264.7 cells.Results : The extracts from stem part showed better DPPH scavenging activity compared to those of the leaf and root extracts. Their IC₅₀s were measured as 7.17, 144.40 and 165.07 ug/ml, respectively. These results were similar to that of ABTS radical scavenging assay and reducing power. Also, Persicaria tinctoria showed the protective effects of DNA damage against oxidative stress and anti-inflammatory effect by suppression of NO production in LPS induced RAW264.7 cells.Conclusions : These results showed that various parts of Persicaria tinctoria can be used as an effective source of antioxidants and anti-inflammatory agents via antioxidative activities and anti-inflammatory effect.

• 동의생리병리학회지
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• 제21권6호
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• pp.1415-1423
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• 2007

Carbon tetrachloride ($CCl_4$)-induced liver injury depends on a toxic agent that has to be metabolized by the liver NAPDH-cytochrome P450 enzyme system to a highly reactive intermediate. Alternations in the activity of cytochrome P450 enzymes affect the susceptibility to hepatic injury from $CCl_4$. In this study, we evaluated the potential protective activity of the traditional Korean medicinal herb, Corni fructus (CF), against an experimental model of hepatotoxicity induced by $CCl_4$. The CF exhibited a hepatoprotective activity against $CCl_4-induced$ liver damage in Sprague-Dawley (SD) rats, as measured by GOT, GPT, ALP and histological observation. The CF also showed significant decrease of malodialdehyde (MDA) and increase of glutathion (GSH), catalase activity in rat liver homogenate. In addition, the expression of CYP2E1, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, was significantly decreased in the liver of CF treated SD rats. But $CCl_4$ and CF has no significant effect on 1A1 and 3A1 isoform of cytochrome P450. Based on these findings, it is suggested that hepatoprotective effects of CF possibly related to antioxidative effects and regulation of CYP2E1 expression.

• 생약학회지
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• 제50권2호
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• pp.141-147
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• 2019

The aim of this study was to investigate the potential of Acanthopanax sieboldianum (Makino) Koidz (ACS) as a potent antioxidant and antidiabetic agent. The antioxidative and alpha-glucosidase inhibitory activities were examined using the methanol extracts and solvent fractions from ACS-leaf and ACS-stem. Antioxidative activities were measured by in vitro methods such as DPPH and ABTS radical scavenging activity and superoxide dismutase (SOD) activity. When the chloroform and ethyl acetate fractions of ACS-leaf and ethyl acetate fractions of ACS-stem were compared with the control, the SOD-like activity was impaired even at the low treatment concentrations. In addition, the ethyl acetate fractions of ACS-leaf and ACS-stem showed alpha-glucosidase inhibition activities at low treatment concentrations. Analysis of the major components in the fractions of ACS-leaf and ACS-stem was also performed using HPLC. Finally, astragalin, isoqurecetin, chlorogenic acid and caffeic acid contents were measured. Based on this work, we propose that ACS-leaf and ACS-stem have great potential as natural antioxidant and antidiabetic materials related to health benefits.

• 대한임상검사과학회지
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• 제52권2호
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• pp.150-157
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• 2020

본 연구는 이산화망간(MnO₂)의 신경독성과 이에 대한 노박 덩굴 추출물의 보호 효과를 C6 glioma 세포를 배양하여 조사하였다. 본 연구를 위하여 세포생존율을 비롯하여 지질산화(LP) 억제능 및 XO 저해능과 같은 항산화 분석을 시행하였다. 그 결과 MnO₂는 배양 세포에 처리한 농도에 비례하여 세포생존율을 유의하게 감소시켰으며, 이 과정에서 XTT₅₀값은 146.7 μM로 나타나 Borenfreund and Puerner의 독성판정기준에 따라 중간독성(mid-cytotoxic)인 것으로 나타났다. 또한, 항산화제의 일종인 kaempferol (KAE)은 MnO₂의 독성에 의해 손상받은 세포의 생존율을 유의하게 증가시킴으로써 MnO₂의 독성을 방어하였다. 한편, MnO₂ 독성에 대한 노박덩굴(CO) 추출물의 영향 조사에 있어서, CO 추출물은 배양 세포에 MnO₂만을 처리한 것에 비하여 세포생존율을 유의하게 증가시킴으로써 MnO₂의 독성을 효과적으로 방어하였다. 이와 동시에 높은 LP 억제능과 XO 저해능과 같은 항산화 효과를 보여주었다. 이상의 결과에서 MnO₂의 독성에 산화적 손상이 관여하고 있음을 알 수 있었으며, CO 추출물은 LP 억제능과 XO 저해능과 같은 항산화능에 의하여 MnO₂의 독성을 효과적으로 방어한 것을 확인였다. 따라서, CO 추출물과 같은 천연소재는 파킨슨병 유발제인 MnO₂와 같이 산화적 손상과 관련된 질환적 중금속 독성을 경감 내지는 개선할 수 있는 유용한 물질이라 생각된다.

• 한국응용과학기술학회지
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• 제28권4호
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• pp.482-490
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• 2011

In this study, the antioxidative effects and inhibitory effects on tyrosinase and elastase of Sorbus commixta (S. commixta) twig extracts were investigated. The aglycone fraction of S. commixta twig extract showed the prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity($FSC_{50}$, $13{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities of S. commixta twig extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated by the luminol-dependent chemiluminescence assay. The 50 % ethanol extract among extracts showed the most prominent ROS scavenging activity ($OSC_{50}$, $0.189{\mu}g/mL$). The cellular protective effects of extract/fractions of S. commixta twig on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The 50 % ethanol extract and ethyl acetate fraction showed the cellular protective effects against ROS in a concentration dependent manner ($5{\sim}50{\mu}g/mL$). The inhibitory effect of S. commixta twig extract on tyrosinase was investigated to assess the whitening efficacy. The ethyl acetate ($IC_{50}$, $113.2{\mu}g/mL$) and aglycone fraction($IC_{50}$, $105.3{\mu}g/mL$) on tyrosinase showed more remarkable inhibitory effect than arbutin($IC_{50}$, $226.88{\mu}g/mL$), known as the whitening agent. The inhibitory effect of aglycone fraction ($IC_{50}$, $6.9{\mu}g/mL$) on elastase was simillar to quercetin($IC_{50}$, $6.1{\mu}g/mL$), flavonoid known as reference compound. These results indicate that extract/fractions of S. commixta twig can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. S. commixta twig extracts can be applicable to new functional cosmetics for anti-aging products.