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Study on the Protective Effect of Corni Fructus against Free Radical Mediated Liver Damage  

Ha, Ki-Tae (Department of Pathology, College of Oriental Medicine, Dongguk University)
Kim, Young-Mi (Department of Pathology, College of Oriental Medicine, Dongguk University)
Kim, Cheorl-Ho (Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University)
Kim, Dong-Wook (Department of Pharmacognostic Resources, College of Natural Science, Mokpo National University)
Choi, Dall-Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University)
Kim, June-Ki (Department of Pathology, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.6, 2007 , pp. 1415-1423 More about this Journal
Abstract
Carbon tetrachloride ($CCl_4$)-induced liver injury depends on a toxic agent that has to be metabolized by the liver NAPDH-cytochrome P450 enzyme system to a highly reactive intermediate. Alternations in the activity of cytochrome P450 enzymes affect the susceptibility to hepatic injury from $CCl_4$. In this study, we evaluated the potential protective activity of the traditional Korean medicinal herb, Corni fructus (CF), against an experimental model of hepatotoxicity induced by $CCl_4$. The CF exhibited a hepatoprotective activity against $CCl_4-induced$ liver damage in Sprague-Dawley (SD) rats, as measured by GOT, GPT, ALP and histological observation. The CF also showed significant decrease of malodialdehyde (MDA) and increase of glutathion (GSH), catalase activity in rat liver homogenate. In addition, the expression of CYP2E1, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, was significantly decreased in the liver of CF treated SD rats. But $CCl_4$ and CF has no significant effect on 1A1 and 3A1 isoform of cytochrome P450. Based on these findings, it is suggested that hepatoprotective effects of CF possibly related to antioxidative effects and regulation of CYP2E1 expression.
Keywords
Corni Fructus; cytochrome P450 2E1; carbon tetrachloride; hepatoprotection; antioxidative effect;
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