• 제목/요약/키워드: antioxidation

검색결과 426건 처리시간 0.021초

권백 추출물의 인간 피부 섬유아세포에서의 항산화와 Matrix Metalloproteinase-1 발현저해효과 (Effect of the Selaginella tamariscina Extract on Antioxidation and Inhibition of Matrix Metalloproteinase-1 in Human Skin Fibroblasts)

  • 심관섭;김진화;박성민;이범천;윤여표;표형배
    • 약학회지
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    • 제48권2호
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    • pp.165-170
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    • 2004
  • The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of extracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In this paper, to investigate the relationship between aging and Selaginella tamariscina extract (STE), we investigated the effects of antioxidant and expression of UVA-induced MMP-1 in human dermal fibroblasts. STE was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the $IC_{50}$/ values of 65.1 $\mu\textrm{g}$/$m\ell$ against 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and 40.9 $\mu\textrm{g}$/$m\ell$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. UVA induced MMP expression was reduced 75.5% by treatment with STE, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore STE was able to significantly inhibition of MMP expression in protein and mRNA level. All these results suggested that STE may act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.

$CCl_4$ 유도 간 독성에 대한 동충하초의 항산화 효과 (Effects of Cordyceps Sinensis and Cordyceps Militaris on Antioxidation in liver of $CCl_4$-treated rats)

  • 민건우;박종혁;신상국;윤철호;서운교;정지천;한영환;신억섭
    • 대한한의학회지
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    • 제22권3호
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    • pp.31-41
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    • 2001
  • Objectives : This study was performed to investigate the effects of Cordyceps Sinensis (CS) and Cordyceps Militaris (CM) on anti oxidation in the livers of ${CCl_4}-treated$ rats. Methods : Hepatotoxicity in rats was induced by carbon tetracWoride. $CCl_4-induced$ rats were administered with the extract of CS and CM. Results : In vitro, CS and CM didn't affect levels of lipid peroxide and the activities and type conversion ratio of xanthine oxidase. However, hydroxyl radicals and DPPHL radicals were decreased. In vivo, in the ${CCl_4}-treated$ rats, lipid peroxide, the activities and type conversion ratio of xanthine oxidase and superoxide radicals were increased but superoxide dismutase was decreased. After CS and CM were administered to ${CCl_4}-treated$ rats, levels of lipid peroxide, the activities and type conversion ratio of xanthine oxidase and superoxide radicals were decreased but superoxide dismutase was increased. Conclusions : These results suggest that CS and CM decrease the activities of free-radical-generating enzymes which form lipid peroxide and increase the activities of oxygen free radical scavenging enzymes.

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Effects of MOK, a pharmacopuncture medicine, on the TH1/TH2 immune response and antioxidation in Con A-stimulated primary mouse splenocytes

  • Hwang, Ji Hye
    • Journal of Acupuncture Research
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    • 제34권2호
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    • pp.39-48
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    • 2017
  • Objectives : In this study, we investigated the immunomodulatory and antioxidant effect of MOK, a pharmacopuncture medicine, in concanavalin A (Con A)-stimulated mouse splenocytes. Methods : Primary splenocytes were isolated from ICR mice. The splenocytes were treated with MOK extract (1.25, 2.5, 5, 10, and 20 mg/mL) for 30 min and then stimulated with Con A (200 ng/mL) for the indicated times. Cell viability of the splenocytes was measured using an MTT assay. The mRNA expression of Th1/Th2 cytokines ($IFN-{\gamma}$, IL-4, IL-10, and Foxp3) and antioxidant enzymes (HO-1 and MnSOD) was measured by RT-PCR. Results : Addition of MOK extract at 2.5, 5, and 10 mg/mL in Con A-stimulated splenocytes significantly decreased the production of $IFN-{\gamma}$ and significantly increased the expression of IL-4, IL-10, and Foxp3 mRNA. MOK extract also increased the mRNA expression of HO-1 and MnSOD in splenocytes. Conclusion : MOK extract modulated the Th1/Th2 immune response via the regulation of cytokine levels in splenocytes and exerted an antioxidant effect via the upregulation of antioxidant proteins.

삼출건비탕가미방(蔘出健脾湯加味方)이 흰쥐의 성장에 미치는 영향 (The Effect of Samchoolgunbitanggamibang of the growth of rats)

  • 백정한;구진숙
    • 대한한방소아과학회지
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    • 제20권1호
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    • pp.49-67
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    • 2006
  • Objectives : The purpose of this study is to find out the effect of antioxidation related to aging of Cheongeumyeonsudan which is written on Dongui-bogam experimentally. Method : 14 weeks aged SD albino rats were separated into uncontrolled group, controlled group and CGY group. As controlled and CGY groups were induced aging by subcutaneous injection of D-galactose, at the same time we administered the extract of Cheongeumyeonsudan to CGY group for 6 weeks. After then we drew blood from each group, and took measurements; the activity of SOD, GSH-px, catalase in erythrocytes, TBARS value, concentration of total lipid, tryglycende, total cholesterol, HDL-cholesterol in blood plasma. Results : The activities of SOD, GSH-px in erythrocytes were significantly increased in the CGY group compared with control group. The activity of catalase showed a tendency to increase, but it was nor remarkable. The concentration of total lipid, the values of TBARS and total cholesterol was significantly decreased in the CGY group compared with control group, and the concentration of plasma HDL-cholesterol was not remarkable. The concentration of tryglycende in plasma showed a tendency to decreased. Conclusions : it is suggested that Cheongeumyeonsudan decreased the activities of free radical, the concentration of lipid in plasma and generate enzyme which form lipid peroxide.

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현토고본환이 노화유발 백서의 항산화능에 미치는 영향 (Effect of Hyuntogobon-hwan on Antioxidation Activity in Induced Aging Rats)

  • 최우석;강석봉
    • 대한한방내과학회지
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    • 제25권4호
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    • pp.147-157
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    • 2004
  • Objectives : This experiment was done to evaluate the effects of Hyuntogobon-hwan(HTG) on antioxidant capability and lipidic concentration in blood, both of which are presumed to be related to aging. Methods : 12 week-old SD rats were divided into controlled group, uncontrolled group and HTG group. As controlled and HTG groups were given subcutaneous injection of D-galactose(50mg/kg/rat), at the same time HTG group was administered extract of Hyuntogobon-hwan(270mg/200g). HTG injections continued for 6 weeks. After initial injections, blood was drawn from each group and the following were measured: the activity of SOD, GSH-px, catalase in erythrocytes, TSARS value, concentration of total lipid, tryglyceride in blood plasma. Results : The activities of SOD and GSH-px in erythrocytes increased significantly in HTG group compared with controlled group. The activity of catalase seemed to increase slightly, but it was barely noticeable. The concentration of total lipid in plasma decreased significantly in HTG group compared with controlled group. The value of TBARS in plasma seemed to decrease slightly, but it was barely noticeable. Conclusions : According to the above results, Hyuntogobon-hwan has an influence on aging by virtue of activation of antioxidative enzyme systems in erythrocytes and concentrations of lipid in blood plasma.

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Differential antioxidation activities in two alfalfa cultivars under chilling stress

  • Wang, Wen-Bin;Kim, Yun-Hee;Lee, Haeng-Soon;Deng, Xi-Ping;Kwak, Sang-Soo
    • Plant Biotechnology Reports
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    • 제3권4호
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    • pp.301-307
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    • 2009
  • To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at $10^{\circ}C$. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

수산건제품의 갈변에 관한 연구 붕장어육 및 유의 산화, 갈변 물질의 성상 (Studies in Browning Reaction in Dried Fish Lipid Oxidative Browning in Dried Conger eel and Properties of Browning Products)

  • 서재수;이강호
    • 한국수산과학회지
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    • 제27권5호
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    • pp.454-461
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    • 1994
  • This studies was carried out in order to investigate the browning reaction of lipid originated compound with nitrogenous compound in dried conger eel. The major fatty acids were $C_{16:0},\;C_{16:1},\;C_{18:1},\;C_{20:5}\;and\;C_{22:6}$. The nonpolar lipid contained the highest percentage of $C_{16:0}$, while the polar lipid contained the highest percentage of $C_{22:6}$. The browning reaction there was a rapidly developed with the beginning of the decline in carbonyl value and remarkable decrease in polyunsaturated fatty acids such as $C_{20:5},\;C_{22:5},\;C_{22:6}$ compared with the other fatty acid, in the water soluble fraction of the browning product obtained from tile fish was detected some antioxidation activity but in the lipid soluble fraction which covers most of the browning reactions in the fish meat antioxidation activity was not detected. In the test of conger eel oil, the phosphatidylcholine was largest in quantity and browning products provided in this experiment showed very low reducing activity.

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쪽파(Allium wakegi Araki)의 아미노산 분석과 항산화 활성 (Amino Acid Analysis and Antioxidation Activity in Allium wakegi Araki)

  • 류상현;송원섭
    • 한국자원식물학회지
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    • 제17권1호
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    • pp.35-40
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    • 2004
  • 쪽파의 잎과 인경, 2,4-D 0.5, 1.0mg/L가 첨가된 MS배지에서 배양된 캘러스로부터 아미노산 분석과 항산화활성 을 분석하였다. 총아미노산 분석의 함량은 자연조건상태의 잎에서는 59.779 mg% 함유하였으나 2,4-D 0.5mg/L가 첨가된 배지에서 배양된 캘러스에서는 73.725 mg%를 함유하였으며 그 가운데 Glutamic acid가 26.555 mg%로서 가장 높게 나타났다. 총아미노산 함량에 대한 필수 아미노산의 함량 비율은 자연조건상태의 인경부분이 39.462 mg%를 함유하였고, 2,4-D 0.5mg/L가 첨가된 배지에서 배양된 캘러스에서는 13.072 mg%를 함유하고 있었다.

Triazine Thiol 유도체(誘導體)에 의한 1-Chlorobutadiene-Butadiene Copolymer의 안정화효과(安定化效果)에 관한 연구(硏究) (A Study on the Stabilization Effects of 1-Chlorobutadiene-Butadiene Copolymer by Triazine Thiol Derivative)

  • 유종선;산하진삼;백남철
    • Elastomers and Composites
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    • 제22권2호
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    • pp.109-120
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    • 1987
  • In this study, as one of the developing ways of the functional elastomer, improvement of the functionality of CB-BR was attemped through stabilization. At first the stabilization effect of CB-BR and the concentration dependancy in CB-BR were determined. Then, triazine thiol derivative(BPTT) was synthesized by reacting p-aminodiphenylamine with cyanuric chloride. Further the functional mechanism and the effects of the antioxidants were investigated using BPTT together with other various antioxidants in liquid and solid states. The results obtained are as follows: 1) The aging of CB-BR depended on the concentration and temperature. Thus, at a low temperature of $50^{\circ}C$, the aging proceeded with gel formation; at high temperature above $100^{\circ}C$ and in above 4wt% concentration, the aging occured by the formation of gel. And in concentrations below that, the aging proceeds with a decomposition caused by oxygen attacked to elastomer molecules. 2) The effect of antioxidation of CB-BR in the liquid state was at it's best when the MBIZ and BPTT were used at $110^{\circ}C$, 4hrs after the oxidation. 3) The effect of antioxidation of CB-BR in the solid state was the best choice the simultaneous use of NDBC and BPTT at $50^{\circ}C$, 30days after the oxidation.

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삼나무와 편백나무 정유의 미백 및 항산화 효능 평가 (Whitening and Antioxidant Activities of Essential Oils from Cryptomeria japonica and Chamaecyparis obtusa)

  • 김선홍;이수연;홍창영;곽기섭;여환명;이전제;최인규
    • Journal of the Korean Wood Science and Technology
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    • 제39권4호
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    • pp.291-302
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    • 2011
  • 본 연구는 삼나무와 편백나무 정유의 tyrosinase 저해 활성과 DPPH (1,1-diphenyl-2- picryl-hydrazyl) radical 소거 활성, SOD (superoxide dismutase) 유사활성을 구명하여 정유의 미백 및 항산화 효능을 평가하였다. 본 연구를 통해 삼나무와 편백나무 정유의 미백 및 항산화 기능성 소재로 개발하기 위한 기초자료로 이용하고, 건조 중 발생하는 nVOCs의 부가적 가치를 확인하고자 하였다. 삼나무 및 편백나무 잎의 정유는 수증기 증류법으로 24시간 추출하였고, 목부는 고온 열처리기를 이용하여 80, 100, $120^{\circ}C$의 온도조건에서 열기 건조하여 건조 중 목부로부터 발생하는 부산물인 천연 휘발성 유기화합물(nVOCs, Natural Volatile Organic Compounds)을 응축액의 형태로 추출하였다. 삼나무 잎의 정유가 편백나무 잎의 정유 보다 미백 및 항산화 활성에서 모두 높은 효과를 나타내었고 건조 시 발생한 nVOCs는 온도가 올라갈수록 미백 활성이 높아지는 것을 확인할 수 있었으며 항산화 활성은 $120^{\circ}C$에서 발생한 삼나무 nVOCs에서만 나타났다.