Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of MOK, a pharmacopuncture medicine, on the TH1/TH2 immune response and antioxidation in Con A-stimulated primary mouse splenocytes

  • Received : 2017.04.06
  • Accepted : 2017.05.08
  • Published : 2017.05.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In this study, we investigated the immunomodulatory and antioxidant effect of MOK, a pharmacopuncture medicine, in concanavalin A (Con A)-stimulated mouse splenocytes. Methods : Primary splenocytes were isolated from ICR mice. The splenocytes were treated with MOK extract (1.25, 2.5, 5, 10, and 20 mg/mL) for 30 min and then stimulated with Con A (200 ng/mL) for the indicated times. Cell viability of the splenocytes was measured using an MTT assay. The mRNA expression of Th1/Th2 cytokines ($IFN-{\gamma}$, IL-4, IL-10, and Foxp3) and antioxidant enzymes (HO-1 and MnSOD) was measured by RT-PCR. Results : Addition of MOK extract at 2.5, 5, and 10 mg/mL in Con A-stimulated splenocytes significantly decreased the production of $IFN-{\gamma}$ and significantly increased the expression of IL-4, IL-10, and Foxp3 mRNA. MOK extract also increased the mRNA expression of HO-1 and MnSOD in splenocytes. Conclusion : MOK extract modulated the Th1/Th2 immune response via the regulation of cytokine levels in splenocytes and exerted an antioxidant effect via the upregulation of antioxidant proteins.

Keywords

References

  1. Cesta MF. Normal structure, function, and histology of the spleen. Toxicol Pathol. 2006;34(5):455-65. https://doi.org/10.1080/01926230600867743
  2. Romagnani S. Biology of human TH1 and TH2 cells. J Clin Immunol. 1995;15(3):121-9. https://doi.org/10.1007/BF01543103
  3. Murphy KM, Reiner SL. The lineage decisions of helper T cells. Nat Rev Immunol. 2002;2(12):933-44. https://doi.org/10.1038/nri954
  4. Abbas AK1, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature. 1996;383(6603):787-93. https://doi.org/10.1038/383787a0
  5. Korean Acupuncture & Moxibution Soc. The Acupuncture and Moxibution Medicine. Paju: Hanmi medical publishing co. 2016:204-8.
  6. Jung C, Jung JH, Lee MS. Immune Pharmacopuncturology. Chungnam: Kyungrak medical publishing co. 2011:127-33.
  7. Hwang JH, Hwang MS, Park YK. MOK, a Pharmacopuncture Medicine, Reduces Inflammatory Response through Inhibiting the Proinflammatory Cytokine Production in LPS-stimulated Mouse Peritoneal Macrophages. The Acupunct. 2017:34(1):11-21 https://doi.org/10.13045/acupunct.2017070
  8. Kim HJ, Gwan R, Han JW et al. Analysis of physioactivities on OK yakchim. J Immuno-Pharmacopuncture. 2013;2(1):9-16.
  9. The National College of Oriental Medicine Herbology Classroom. Herbology. Seoul: Youngrimsa. 2016:216-8, 221-3, 250-3, 257-8, 273-4, 395-6, 560-2, 617-8.
  10. Kim HJ, Gwan R, Han JW, Jung C. Anti-oxidant and anti-inflammatory effects of V yakchim. J Immuno-Pharmacopuncture. 2013;2(1):1-8.
  11. Hwang JH, Cho HS, Lee HJ et al. Effect of Inhibition Macrophage Migration Inhibitory Factor Activation by Hominis Placenta Herbal Acupuncture on Rheumatic Arthritis. The Acupunct. 2008:25(3):41-51.
  12. Zijlstra FJ, van den Berg-de LangeI, Huygen FJ, Klein J. Anti-inflammatory actions of acupuncture. Mediators Inflamm. 2003;12(2):59-69. https://doi.org/10.1080/0962935031000114943
  13. Cho SY, Yang SB, Shin HS et al. Anti-inflammatory and immune regulatory effects of acupuncture after craniotomy: study protocol for a parallel-group randomized controlled trial. Trials. 2017;18:10. https://doi.org/10.1186/s13063-016-1712-7
  14. Feng Y, Siu K, Wang N et al. Bear bile: dilemma of traditional medicinal use and animal protection. J Ethnobiol Ethnomed. 2009;5(2):1-45. https://doi.org/10.1186/1746-4269-5-1
  15. Li X, Xu Y, Zhang C et al. Protective Effect of Calculus Bovis Sativus on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice. Evid Based Complement Alternat Med. 2015;2015:469506.
  16. Jang SY, Park JW, Bu Y, Kang JO, Kim J. Protective effects of hominis placenta hydrolysates on radiation enteropathy in mice. Nat Prod Res. 2011;25(20):1988-92. https://doi.org/10.1080/14786419.2010.513035
  17. Park SY, Phark S, Lee M, Lim JY, Sul D. Antioxidative and anti-inflammatory activities of placental extracts in benzo[a]pyrene-exposed rats. Placenta. 2010;31(10):873-9. https://doi.org/10.1016/j.placenta.2010.07.010
  18. De D, Datta Chakraborty P, Mitra J et al. Ubiquitin- like protein from human placental extract exhibits collagenase activity. PLoS One. 2013;8(3):e59585. https://doi.org/10.1371/journal.pone.0059585
  19. Park JY, Lee JY, Jeong MS et al. Effect of Hominis Placenta on cutaneous wound healing in normal and diabetic mice. Nutr Res Pract. 2014;8(4):404-9. https://doi.org/10.4162/nrp.2014.8.4.404
  20. Pan C, Kumar C, Bohl S, Klingmueller U, Mann M. Comparative Proteomic Phenotyping of Cell Lines and Primary Cells to Assess Preservation of Cell Type-specific Functions. Mol Cell Proteomics. 2009;8(3):443-50. https://doi.org/10.1074/mcp.M800258-MCP200
  21. Iwashima S, Ozaki T, Maruyama S et al. Novel culture system of mesenchymal stromal cells from human sub-cutaneous adipose tissue. Stem Cells Dev. 2009;18(4):533-43. https://doi.org/10.1089/scd.2008.0358
  22. Kubo N, Narumi S, Kijima H et al. Efficacy of adipose tissue-derived mesenchymal stem cells for fulminant hepatitis in mice induced by concanavalin A. J Gastroenterol Hepatol. 2012;27(1):165-72. https://doi.org/10.1111/j.1440-1746.2011.06798.x
  23. Mizuhara H, Kuno M, Seki N et al. Strain difference in the induction of T-cell activation-associated, interferon gamma-dependent hepatic injury in mice. Hepatology 1998;27(2):513-9. https://doi.org/10.1002/hep.510270227
  24. Sun JC, Lanier LL. NK cell development, homeostasis and function: Parallels with $CD^{+}$ T cells. Nat Rev Immunol. 2011;11(10):645-57. https://doi.org/10.1038/nri3044
  25. Takada K, Jameson SC. Naive T cell homeostasis: From awareness of space to a sense of place. Nat Rev Immunol. 2009;9(12):823-32. https://doi.org/10.1038/nri2657
  26. O'Garra A, Robinson D. Development and function of T helper 1 cells. Adv Immunol. 2004;83:133-62.
  27. Barthlott T, Moncrieffe H, Veldhoen M et al. CD25+CD4+ T cells compete with naive CD4+ T cells for IL-2 and exploit it for the induction of IL-10 production. Int Immunol. 2005;17:279288.
  28. Setoguchi R, Hori S, Takahashi T, Sakaguchi S. Homeostatic maintenance of natural Foxp3+ CD25+ CD4+ regulatory T cells by interleukin (IL)-2 and induction of autoimmune disease by IL-2 neutralization. JExpMed. 2005;201(5):723-35. https://doi.org/10.1084/jem.20041982
  29. Wan YY, Flavell RA. Regulatory T-cell functions aresubvertedandconvertedowingtoattenuated Foxp3 expression. Nature. 2007:445(7129):766-70. https://doi.org/10.1038/nature05479
  30. Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and non-self. Nat Immunol. 2005;6(4):345-52. https://doi.org/10.1038/ni1178
  31. Belkaid Y. Regulatory T cells and infection: a dangerous necessity. Nat Rev Immunol. 2007;7(11):875-88. https://doi.org/10.1038/nri2189
  32. Davies KJ. Oxidative stress: the paradox of aerobic life. Biochem Soc Symp. 1995;61:1-31. https://doi.org/10.1042/bss0610001
  33. Sareen SG, Jack LS, James LG. The antioxidant nutrients, reactive species, and disease. In: Advanced nutrition and human metabolism 4th edited. California, USA: Thomson Wadsworth. 2004:368-77.
  34. LeopoldJA, LoscalzoJ. Oxidativemechanismsand atherothrombotic cardiovascular disease.Drug Discov Today Ther Strateg. 2008;5(1):5-13. https://doi.org/10.1016/j.ddstr.2008.02.001
  35. Frankel D, Mehindate K, Schipper HM. Role of heme oxygenase-1 in the regulation of manganese superoxide dismutase gene expression in oxidatively-challenged astroglia. J Cell Physiol. 2000;185(1):80-6. https://doi.org/10.1002/1097-4652(200010)185:1<80::AID-JCP7>3.0.CO;2-W

Cited by

  1. Therapeutic effects of acupuncture with MOK, a polyherbal medicine, on PTU-induced hypothyroidism in rats vol.16, pp.1, 2017, https://doi.org/10.3892/etm.2018.6190
  2. Jiang Zhi Granule protects immunological barrier of intestinal mucosa in rats with non-alcoholic steatohepatitis vol.59, pp.1, 2017, https://doi.org/10.1080/13880209.2021.1979594