• 대한본초학회지
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• 제31권6호
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• pp.45-52
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• 2016

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were $24.6mg/m{\ell}$ and $14.3mg/m{\ell}$, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.

• 동아시아식생활학회지
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• 제21권5호
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• pp.677-682
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• 2011

This study explores the antioxidant activities of solvent extracts from Rhus verniciflua, and the results were as follows. Total phenolic compound and flavonoid contents of Rhus verniciflua were $4.32{\pm}0.17$ mg/100 g and $0.16{\pm}0.02$ mg/100 g, respectively. Electron-donating abilities of solvent extracts from Rhus verniciflua proportionally increased with concentration, and ethyl acetate extract ($95.4{\pm}0.4%$) showed higher activity than that of BHT ($93.3{\pm}1.7%$) at a concentration of 1,000 ${\mu}L$/mL. Reducing power of the solvent extracts from Rhus verniciflua was the highest in ethyl acetate extract ($2.45{\pm}0.03$) at a concentration of 1,000 ${\mu}L$/mL. Nitrite scavenging ability of the solvent extracts from Rhus verniciflua (pH 1.2, 1,000 ${\mu}L$/mL) was the highest in ethyl acetate extract ($95.5{\pm}0.7%$). Therefore, ethyl acetate extract from Rhus verniciflua has similar antioxidant activity as that of BHT.

• Food Science and Biotechnology
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• 제15권1호
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• pp.143-147
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• 2006

Physiological activities of Korean-grown ginger (KG) and Chinese-grown ginger (CG) extracts were examined. Ginger was extracted with water, and 50 and 100% ethanol, and then nitrite-scavenging activity (NSA), superoxide dismutase (SOD)-like activity, and electron-donating ability (EDA) of extracts were investigated. NSA at pH 1.2 was the most effective in 50% ethanol extracts of both origins. SOD-like activities of water and 50% ethanol extracts of both samples were 8.66-35.95% lower than those of 1 and 0.1% L-ascorbate solutions. SOD-like activity of KG extracts was higher than that of CG extracts, and water extracts of samples were the highest. EDA of KG extract was higher (22.23-86.95%) than that of CG extract, while both sample extracts showed lower EDA than those of 1 and 0.1% L-ascorbate solutions.

• 한국자원식물학회지
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• 제24권3호
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• pp.314-318
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• 2011

Saururus chinensis is used as a raw material of an anti-rheumatic, poultice, sedative and stomachic. The tea of dried Saururus chinensis leaves has a function of relaxing pains in back and breast and of treating stomach ailments. The plant has the functions of anti-inflammatory, depurative, diuretic, febrifuge and refrigerant. The extraction of functional material is carried out by methanol and ethanol solvents. Mechanochemical grinding is applied as a pretreatment process before extraction to enhance the extraction efficiency by increasing the surface area of the materials while changing the chemical properties of the materials. Extraction of functional materials from the Saururus chinensis after grinding as a pretreatment using the mechanochemical technology was accomplished in this study to investigate the effect of grinding on the antioxidant activities of the extract. Saururus chinensis was ground by the planetary ball mill and the morphology was analyzed by SEM. Mechanochemical pretreatment increased the yields of functional materials from 5.9 g to 6.4, 7.0, and 8.1 g after grinding of 30 minutes, 1 hour and 2 hours, respectively. Nitrite scavenging ability increased from 53.0-71.0% to 61.0-79.0% for the methanol extraction in the solution of pH 1.2. Also, Electron donating abilities were increased from 7.90-33.51% to 11.97-38.51% for the methanol extraction. The extract concentration for the half inhibition of DPPH radicals was reduced from $278{\mu}g/mL$ in the original sample to $263{\mu}g/mL$ in the sample after grinding for the methanol extraction.

• Food Science and Biotechnology
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• 제15권4호
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• pp.582-588
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• 2006

The antioxidant and antimicrobial activity of the fermentation extract (PFE) and the 50 and 80% ethanol extracts (PE 50, PE 80) of Pinus densiflora pine needles were evaluated. Electron donating ability, superoxide dismutase (SOD) ability, and antimicrobial activity were observed in PFE; those abilities differed in PE 80 and PE 50, depending on the ethanol concentration used for the extraction. PFE had the highest electron donating ability with a value of 92.20%, while PE 80 and PE 50 had values of 74.66 and 53.47%, respectively. For SOD activity, PE 80 exhibited a slightly higher value of 31.11% compared to that of PFE and PE 50, which were 29.65 and 25.43%, respectively. PFE, PE 50, and PE 80 were all found to inhibit bacteria, and the effectiveness of this inhibition was strongly related to the type of extracts used. PFE showed good antimicrobial effects for all of the tested Gram-positive strains and for most of the tested Gram-negative strains. These results suggest that PFE has superior functionality compared to the ethanol extracts (PE 80, PE 50), in terms of antioxidant and antimicrobial activity. On the basis of these results, pine needle fermentation extracts can be used for industrial applications as a functional material.

• 한국식품과학회지
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• 제36권3호
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• pp.461-464
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• 2004

매실의 항산화력을 알아보기 위해 매실과육과 매실즙 및 이들의 수용성 추출물을 시료로 하여 과산화물가, TBA가 및 전자공여능을 측정하였다. 과산화물가는 저장 4주 후 매실과즙 추출물의 경우 226.55 meq/kg으로 항산화제로 알려져 있는 BHT(217.38meq/kg) 및 ascorbic acid(223.32meq/kg)와 유사한 값을 나타내 이들과 유사한 항산화력을 갖는 것으로 분석되었다. TBA가를 통해 항산화력을 측정한 결과 합성산화제인 BHT가 45.35%로 가장 높은 항산화력을 보였고 매실과즙 추출물은 25.0%,로 29.94%를 나타낸 ascorbic acid와 유사한 수준의 값을 나타내었다. DPPH를 이용한 전자공여능을 측정한 결과 0.01% 매실과육 추출물은 34.84%, 0.01% 매실과즙 추출물 43.50%를 보였으며 0.02% 매실과육 추출물은 53.21%, 0.02% 매실과즙 추출물은 59.19%로 증가하는 양상을 나타내었으나 BHT(77.82%)와 ascorbic acid(96.69%)에 비해 낮은 활성을 나타내었다. 이상의 결과로 볼 때 매실과즙 추출물의 경우 BHT와 ascorbic acid와 유사한 항산화성을 나타내 천연 항산화제로의 이용 가능성이 있다고 생각되어진다.

• 한국식품영양학회지
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• 제28권3호
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• pp.458-464
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• 2015

본 연구에서는 진피의 활용도를 높이기 위한 연구의 일환으로 유기용매별에 따른 생리활성물질의 용출량을 측정하기 위해, 진피와 에탄올 진피 추출물을 대상으로 유기용매인 에틸 아세테이트, 아세톤, 염화 메틸렌, 메탄올을 이용하여 추출한 시료를 대상으로 총 폴리페놀 함량, 전자공여능, glutathione S-transferase(GST)의 활성 저해력을 측정한 결과는 다음과 같다. 1. 총 폴리페놀 함량은 ethyl acetate인 경우 진피는 $928.48{\pm}1.19{\mu}g\;GAE/mL$, 에탄올 추출 진피는 $664.64{\pm}0.74{\mu}g\;GAE/mL$로, acetone인 경우 진피는 $886.03{\pm}0.44{\mu}g\;GAE/mL$, 에탄올 추출 진피는 $702.67{\pm}0.85{\mu}g\;GAE/mL$로, methylene chloride인 경우 진피는 $413.08{\pm}1.39{\mu}g\;GAE/mL$, 에탄올 추출 진피는 $429.64{\pm}0.61{\mu}g\;GAE/mL$로, methanol인 경우 진피는 $12,648.60{\pm}0.56{\mu}g\;GAE/mL$, 에탄올 추출 진피는 $16,108.20{\pm}0.73{\mu}g\;GAE/mL$로 나타나, 진피나 에탄올 추출 진피는 모두 methanol로 추출한 것이 상대적으로 높게 나타났으며, 총 폴리페놀의 함량 차이는 유기용매별 통계적으로 유의한 차이가 나타났다(p<0.05). 2. 전자공여능은 ethyl acetate인 경우 진피는 62.80%, 에탄올 추출 진피는 51.49%로 나타났으며, acetone인 경우 진피는 97.43%, 에탄올 추출 진피는 63.17%로 나타났으며, methylene chloride인 경우 진피는 52.20%, 에탄올 추출 진피는 67.68%로 나타났으며, methanol인 경우 진피는 97.63%, 에탄올 추출 진피는 96.18%로 나타났다. Electron donating ability(EDA)는 유기용매 중 메탄올로 추출하였을 때가 상대적으로 가장 높게 나타났으며, 유기용매별로 통계적으로 유의한 차이가 나타났다(p<0.05). 3. Glutathione S-transferase(GST)에 대한 활성 저해능은 ethyl acetate인 경우 진피는 76.22%, 에탄올 추출 진피는 75.54%로 나타났으며, methylene chloride인 경우 진피는 31.73%, 에탄올 추출 진피는 73.53%로 나타났으며, methanol인 경우 진피는 97.48%, 에탄올 추출 진피는 48.70%로 나타났다. Glutathione S-transferase(GST)에 대한 활성 저해능은 진피인 경우 유기용매 중 메탄올로 추출하였을 때가 가장 높게 나타났고, 에탄올 추출 진피인 경우는 에탄올과 methylene chloride로 추출할 때가 높게 나타났으며, 유기용매별로 통계적으로 유의한 것으로 나타났다(p<0.05).

• 한국식품영양학회지
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• 제21권1호
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• pp.35-42
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• 2008

To develop physiological functionality of Omija extracted with water was evaluated on antioxidative activity. Omija, high acid material with pH 3.6, contain $57.5{\pm}1.03%$ of moisture, and $18.8{\pm}0.12%$ of crude fat. This material have $12.6{\pm}0.04%$ of carbohydrate and $11.1{\pm}0.07%$ of crude protein as well, but ash and crude protein contents were found less than 10%. 10 mineral contents were also found, too; K and Ca showed the highest level, then Al, Mg, Na and Mn were followed. In composition amino acid contents, glutamic acid took the largest portion, $131.7{\pm}1.3$ mg/100 g, aspartic acid $51.5{\pm}0.6%$, and other composition amino acid under 50%. In case of free sugar contents, 7 types were found. Most of them were glucose and fructose. Total phenolic compounds showed the highest level, $2,862.6{\pm}31.7$ mg/100 g. $197.8{\pm}14.6$ mg/100 g of flavonoid and $225.6{\pm}18.2$ mg/100 g were included. In terms of electron donating ability, radical scavenging ability activated as the amount of Omija extract increased. In particular, Omija extract in 1,000 ${\mu}g/m{\ell}$ demonstrated almost similar electron donating ability, $72.4{\pm}0.21%$, to BHT. It was also found that antioxidant activities of electron donating ability, SOD-like ability, hydroxyl radical scavenging ability and nitrite scavenging ability were highly promoted as Omija extract concentration increased. The nitrite scavenging ability was significant when the extract belonged to strong acid region and doping concentrations increased.

• 한국식품영양학회지
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• 제21권3호
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• pp.312-319
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• 2008

The principal objective of was to assess the effects of herb extracts on the antioxidant activity and quality characteristics of Maejakgwa. The total phenolic and flavonoid contents were found to be the highest by a significant degree in the rosemary, lavender, thyme, lemon balm and olive samples, in order. With regard to electron donating ability, increasing concentrations of added herbs resulted in a more significant rise in the DPPH radical scavenging effect in a range of concentrations of between $100{\sim}1000{\mu}g/m{\ell}$. In particular, the rosemary, lavender, and thyme samples were shown to be lower in antioxidant activity than ascorbic acid, but higher than a-tocopherol, thus the antioxidant activities detected in the rosemary, lavender, and thyme samples were considered to be excellent. With regard to reducing ability, increases in the concentration of added herb extract resulted in significant growth. However, the anti-oxidative activity detected in the rosemary extract was indicated to be superior even to that of $\alpha$-tocopherol when added to concentrations of $500{\sim}1,000{\mu}g/m{\ell}$. In our assessment of external appearance, color, flavor, texture, and overall quality, the highest preference was seen in the control group and in the group with 2% added rosemary extract. The lowest score was earned in the group with a 5% addition of rosemary. The functional ingredients and antioxidant activities of the samples were excellent. Our results appear to indicate that rosemary has some value as a natural antioxidant that can prevent oxidation in food.

• 한국식품조리과학회지
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• 제23권1호통권97호
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• pp.19-24
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• 2007

This study investigated the antioxidant and synergistic effects and nitrite scavenging ability of some phenolic compounds(catechin, rutin, quercetin and naringin), The electron donating abilities of naringin, quercetin, rutin and catechin were 6.7%, 92.8%, 87.6% and 92.21%, respectively, The antioxidant activities in O/W emulsion substrates were in order of rutin > quercetin > catechin > naringin. The antioxidant effect of rutin was stranger than that of BHT or ${\alpha}$-tocopherol. ${\alpha}$-tocopherol showed synergistic effect with catechin and quercetin, but ascorbic acid not showed effect. The nitrite scavenging abilities of catechin, quercetin, rutin and naringin were 99.9%, 98.6%, 25.5% and 0.2%, respectively. The nitrite scavenging abilities of quercetin and actechin were very potent as compared with those of BHT and ascorbic acid.