• Title/Summary/Keyword: antifungal effects

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Anti-Aspergillus Activities of Plant Essential Oils and Their Combination Effects with Ketoconazole or Amphotericin B

  • Shin, Seung-Won
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.389-393
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    • 2003
  • The essential oils from Cedrus atlantica, Styrax tonkinensis, Juniperus communis, Lavandula angustifolia, Melaleuca alternifolia, Pelargonium graveolens, Pogestemon patchouli and Rosmarinus officinalis were analyzed by GC-MS. Antifungal activities of the oils were investigated by disk diffusion assay and the broth dilution method against Aspergillus niger and A. flavus. The effects of geraniol and the essential oil fraction from P. graveolens on the antifungal activity of amphotericin Band ketoconazole were examined using a checkerboard microtiter assay against both Aspergillus fungi. Most of the tested essential oils, with the exception of C. atlantica, J. communis, and P. patchouli, significantly inhibited growth of A. niger and to a lesser extent that of A. fIavus, with MICs (minimal inhibitory concentrations) in the range 0.78-12.5 mg/mL. The essential oil fraction of P. graveolens and its main components, geraniol and citronellol, exhibited additive effects with amphotericin B and with ketoconazole against both Aspergillus species, resulting in fractional inhibitory concentration (FIC) indices ranging from 0.52 to 1.00.

Experimental Study on Antifungal effects of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortex and those mixture, Phellodendri Cortex, Sophorae Radix, Torilis Fructus on various species of the dermatophytes (足部白癬茵의 韓方治療劑 開發을 위한 硏究)

  • Choi, Gyu-dong;Kwon Young kyu, Yeong-Gyu
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.13 no.1
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    • pp.267-279
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    • 2000
  • Minimum inhibitory concentration(MIC) of Sophorae Subprostratae Radix. Aconiti Radix, Hibisci Syriaci Cortex and those mixture. Phellodendri Cortex, Sophorae Radix, Torilis Fructuson on various species of the dermatophytes on Microsporium gypseum, Trichophytone rubrum and Epidermophytone floccosum were determined after 4 weeks of incubation at $25^{\circ}C$ in Sabouraud dextrose broth. The results were summarized as followes : 1. Antifungal effects of Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortex on T rubrum which is the most common organism of tinea pedis were more effective than that of the others. 2 These results suggested that herbal medicines such as Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortexse can be used to treat tinea pedis and to improve untoward effects associated with the administration of antifugual agents.

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Styraxjaponoside A and B, Antifungal Lignan Glycosides Isolated from Styrax japonica S. et Z.

  • Park, Cana;Cho, Jae-Yong;Hwang, Bo-Mi;Hwang, In-Sok;Kim, Mi-Ran;Woo, Eun-Rhan;Lee, Dong-Gun
    • Biomolecules & Therapeutics
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    • v.18 no.4
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    • pp.420-425
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    • 2010
  • The antifungal effects and action mechanisms of styraxjaponoside A and B were investigated. Devoid of hemolytic effect, the compounds had significant effect against several human pathogenic fungal strains, with energy-independent manners. To understand the action mechanisms of the compounds, the flow cytometric analysis plotting the forward scatter and the side scatter, $DiBAC_4$(3) staining and DPH fluorescence analysis were conducted. The results indicated that the actions of the compounds were dependent upon the membrane-active mechanisms. The present study suggests that styraxjaponoside A and B exert their antimicrobial effects via membrane-disruptive mechanisms.

Inhibitory effects of 15 mushroom culture extracts on the growth of Alternaria alternata causing potato brown spot (감자갈색잎점무늬병균(Alternaria alternata)에 대한 버섯 배양액 추출물 15종의 생장 억제 효과)

  • Hyon Jin Park;Ha Thi Kim Nguyen;Sook-Young Park;Jaehyuk Choi
    • Journal of Mushroom
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    • v.21 no.4
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    • pp.195-199
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    • 2023
  • The fungus Alternaria alternata, responsible for causing brown to black spotting on numerous fruits and vegetables globally, was identified in 2022 as the causative pathogen of brown spot disease in potatoes in Korea. In pursuing potential inhibitors against A. alternata growth, we evaluated 15 mushroom culture filtrates: eight from Trametes spp. and seven from Polyporus spp., known for their antibacterial and anticancer properties. Antifungal activity was assessed by exposing each filtrate to A. alternata on a paper disc. Four filtrates displayed inhibitory action against the fungus, albeit with mild effects. Our findings highlight the potential of Trametes and Polyporus fungi as emerging antifungal candidates, offering promise in preventing potato brown spots.

Antifungal Activity of Some Essential Oils and Their Major Constituents on 3 Plant Pathogenic Fungi (식물병원성 곰팡이에 대한 몇 가지 식물정유 및 주성분의 성장억제 효과)

  • Cho Hyun Ji;Shin Dongill
    • Journal of Life Science
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    • v.14 no.6 s.67
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    • pp.1003-1008
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    • 2004
  • 11 plant essential oils are screened in vitro for their antifungal activities against Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani, which are causative agents of serious plant diseases. The radial growth of the test fungi were reduced in response to the oils. Among them, the essential oil from the bark of Cinnamomum zeylanicum inhibited 3 tested fungi growth, strongly, followed by those of oregano and thyme. The major constituents of the three essential oils, cinnaldehyde, carvacrol and thymol were tested for their effects on the fungi. From the results obtained, cinnamaldehyde, the major constituents of C. zeylanicum bark esential oil, has potential to be developed as a biopesticide for controlling phytopathogenic fungi causing serious damages on the important crops cultivated in Korea.

Biological Control of Fusarium Wilt by Antagonistic Microorganism in Greenhouse Grown Cucumber Plants (오이의 온실재배에서 발생하는 위조병의 미생물학적 제어)

  • Cho, Jung-Il;Cho, Ja-Yong
    • Korean Journal of Organic Agriculture
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    • v.12 no.1
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    • pp.101-114
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    • 2004
  • This study was carried out to clarify the effects of antagonistic microorganism, Bacillus sp. JC181 isolated from the greenhouse soil grown cucumber plants on the growth inhibition of plant pathogen, fusarium wilt (Fusarium oxysporum) occurred in cucumber plants in greenhouse. Antagonistic bacterial strains were isolated and were investigated into the antifungal activity of the antagonistic microorganism against fusarium wilt. Screened fourteen bacterial strains which strongly inhibited F. oxysporum were isolated from thc greenhouse soil grown cucumber plants, and the best antagonistic bacterial strain designated as JC181, was finally selected. Antagonistic bacterial strain JC181 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. JC181 showed 58.2% of antifungal activity against the plant pathogen growth of F. oxysporum. By the bacterialization of culture broth and heated filtrates of culture broth, Bacterial strain, Bacillus sp. JC181. showed 91.2% and 260% of antifungal activity against F. oxysporum, respectivrly.

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Allelopathic Effects on Seed Germination and Fungus Growth from the Secreting Substances of Some Plants (수종식물의 분비물질이 종자 발아와 균류 생장에 미치는 알레로파시 효과)

  • 이호준;김용옥;장남기
    • The Korean Journal of Ecology
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    • v.20 no.3
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    • pp.181-189
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    • 1997
  • Phenolic compounds from 7 species of naturalized invader species and Korean wild plants were analyzed by high performance liquid chromatography. Eleven phenolic compounds including benzoic acid were identified. The extract of naturalized plants was significantly more inhibitory to seed germination and seedling growth of the both naturalized and Korean wild plants. The content of total phenolic compounds in each extract were 43.5 mg/l in Ailanthus altissima as the maximum amount and 25.5 mg/l in Phytolacca americana as the minimum. Phytotoxic substances of ethanol extracts was investigated for antifungal activity against 23 selected fungus species. The antifungal activity of Phytolacca americana showed the greatest clear zone of 23 mm in Aspergillus awamori and its activity had an effect against 6 fungus species. Ailanthus altissima formed the greatest clear zone of 26 mm in Erwinia carotovora sub. sp. carotovora and had an effect agsinst 2 fungus species.

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Selection and Bacterialization into Rootzone of the Various Plant Growth Promoting Rhizobacteria in Peatmoss Compost on the Early Growth of Cucumber and Tomato Plug Seedlings (수종의 식물생장촉진 근권미생물의 분리 및 근권처리가 오이와 토마토 플러그묘의 초기생장에 미치는 영향)

  • 조자용;김광수;정순주
    • Korean Journal of Organic Agriculture
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    • v.6 no.1
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    • pp.151-160
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    • 1997
  • Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

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Screening of antifungal activities of Bacillus thuringiensis strains for the development of biocontrol agents of plant diseases

  • Kim, G. H.;Kim, D. S.;Lee, D.H.;J. S. Hur;Y. J. Koh
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.99.2-99
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    • 2003
  • An attempt was made to screen antifungal activities of Bacillus thuringiensis strains on various plant pathogens, Botryosphaeria dothidea, Diaporthe actinidiae, Botrytis cinerea, Glomerella cingulata, Colletorichum cocodes, Sclerotinia scierotiorum, Alternaria alternata, Helicobuidium mompa, Bipolaris coicis, Fusarium graminearum and Rhizoctosnia solani. Ten and forty-five strains of B. thuringiensis were isolated from animal feces in Korea and Japan, respectively. Inhibitory effects of the strains on the mycelial growth of the pathogens were examined on the mixed media of potato dextrose agar and nutrient agar. Approximately half of the strains inhibited the mycelial growth of one or more pathogens. Most of the pathogens were inhibited by any of the strains but Fusarium graminearum and Rhizoctonia solani were not inhibited at all. This is the first report that B. thuringiensis shows a potent antifungal activity on plant pathogens in Korea.

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