• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.429-436
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• 2003

Pediocin K1 is a bacteriocin produced by Pediococcus sp. KCA 1303-10, isolated from traditionally fermented flatfish in Korea. Pediocin K1-dependent antibiosis and pediocin K1-independent antibiosis against Listeria monocyrogenes were investigated by comparing antibiosis potential of the ped＋ wild-type strain of Pediococcus sp. KCA1303-10 with that of the ped- mutant strain in 3 different media at 3 different temperatures. In the synthetic MRS-APT medium, bacteriocin (pediocin K1)-dependent antibiosis (BDA) acted as the major driving force of overall antibiosis at the initial stage before the pH of the media was not sufficiently lowered, while bacteriocin-independent antibiosis (BIA) took over the major role at the late stage of antibiosis by killing otherwise resistant cells in the modium. The role of BDA increased as the temperature of the system decreased. The antibiosis potential of BDA among the overall antibiosis of Pediococcus against Listeria at $37^{\circ}C$ was calculated as 46%, and as 75% at $25^{\circ}C$. In the skim milk medium, antibiosis of Pediococcus against Listeria was weakened more than 4 log cycles compared to that of the synthetic medium; however, BDA worked as the main antibiosis force regardless of the culturing temperature in the skim milk medium. In the bean soup medium, BDA also worked as the major killing mechanism against Listeria, but BIA played as another suppressing mechanism against otherwise pediocin-resistant Listeria population. These results suggest that a large portion of the inhibitory action of the ped+Pediococcus sp. KCA1303-10 was attributable to the bacteriocin produced by the strain and that viable Pediococcus sp. KCA1303-10 was superior to the purified bacteriocin in suppressing the occurrence of the bacteriocin-resistant Listeria monocytogenes in food systems.

• Journal of the Korean Society of Clothing and Textiles
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• v.36 no.3
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• pp.324-334
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• 2012

In this research, the dyeing properties of $Lycopus$ $lucidus$ Turcz on cotton and silk fabrics were evaluated to establish a scientific database of natural dyes. The optimum dyeing conditions were identified with K/S values depending on the dyeing concentration, temperature, time, and pH. For the optimum conditions, the color changes for different synthetic mordants were observed as L, $a^*$, $b^*$ and H, V, C. The color fastness after dyeing and mordanting was also examined. Additionally, the antibiosis of $Lycopus$ $lucidus$ Turcz was examined. The results were as follows: For the cotton, the optimized dyeing conditions were 300% (o.w.f.), $50^{\circ}C$, 40 min., and pH 5. For the silk fabric, the conditions were 450% (o.w.f.), $70^{\circ}C$, 60 min., and pH 4. The K/S value of dyed cotton improved about 1.03-2.78 folds after mordanting. The fabric color was yellow in the absence of mordanting, while it was reddish yellow after post-mordanting with FFC. Although the color changed with the mordanting method and mordant, the overall hue was yellow. Thus, $Lycopus$ $lucidus$ Turcz appears to have the potential for use as a natural dyeing material for a yellow hue. The K/S value of the dyed silk fabric improved about 1.05-1.40 folds after mordanting. The fabric showed a reddish yellow color when pre-mordanted with Al and Cu. The washing and dry-cleaning fastness of the cotton and silk fabrics were at the 4-5 level. The light fastness of the cotton improved after mordanting; however, pre-mordanting was more effective than post-mordanting or the absence of mordanting. The dyed cotton, silk fabrics showed excellent antibiosis.

• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.62-67
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• 1997

A potential biocontrol bacterium, YB-70 was isolated from a rhizosphere in suppressive soil and identified as a strain of Bacillus subtilis. In several biochemical and in vitro antibiosis tests on Fusarium solani with the culture filterates from B. subtilis YB-70, we found that antifungal mechanism of B. subtilis YB-70 was mediated by antibiotic substances produced from the bacterium. These antifungal substances were appeared to be hear-resistant, micromolecular, and ethy alcohol soluble. Antifungal agents produced by B. subtilis YB-70 showed strong inhibified against root-rotting fungi F. solani in in vivo pot test. An antifungal substance. YBS-1s, was purified from the culture broth of B. subtilis YB-70 by isoelectronic precipitation, silica gel column chromatography and Sephadex LH-20 column chromatography analysis by Fab-MASS, $^{1}$H-NMR, $^{13}$C-NMR, DEPT, and amino acid analyzer revealed that the YBS-1A was a peptide antibiotics of iturin class containing seven amino acids from five different groups, and the other(YBS-1B) was an analogue of iturin group composed of 11 amino acids with larher molecular weight of about 1, 500 dalton, which was lager than that of iturin A.

• Mycobiology
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• v.38 no.4
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• pp.286-294
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• 2010

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.

• The Korean Journal of Pesticide Science
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• v.13 no.4
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• pp.275-282
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• 2009

The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.

• Food Science of Animal Resources
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• v.31 no.5
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• pp.741-747
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• 2011

Gardenia fructus has been reported to have bioactivities of lowering blood glucose, antitumor, antithrombosis, repression of neogenesis of blood vessels, antioxidant and antibiosis. However, the nitrite scavenging activity and utilization in meat products have not been studied. The substitution effect for nitrite and antibiosis of Gardenia fructus extract (GFE) were investigated by measuring the residual nitrite contents and storage properties of pork patties prepared with nitrite (50, 100, and 150 ppm) and GFE (0, 0.25, 0.5%). The CIE $L^*$ and CIE $a^*$ of pork patties decreased, while CIE $b^*$ increased as the addition of GFE increased. Patties with more GFE added tended to be lower in pH when stored at $4^{\circ}C$ for 6 wk, but TBARS and VBN were not affected by the addition of GFE. Residual nitrite in patties was lowered as the storage period was lengthened and as the GFE addition was increased. During the storage at $4^{\circ}C$, Escherichia coli was not detected, and the total aerobic bacterial count was decreased as more GFE was added, showing the substitution effect of GFE for nitrite in antimicrobial activity. In conclusion, the results show that GFE has nitrite scavenging and antibiotic activities in meat products, suggesting its potential use in healthy and sustainable foods with diverse biofunctionalities.

• The Plant Pathology Journal
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• v.23 no.1
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• pp.22-25
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• 2007

Biocontrol activity of five strains of selected rhizo-bacteria were tested in tomato against bacterial wilt caused by Ralstonia solanacearum. After root bacterization the plants were grown in a perlite-hydroponic system. Upon challenge inoculation with the pathogen, all of the rhizobacterial strains efficiently suppressed the bacterial wilt in tomato in various rates, at maximum by the strain, Bacillus vallismortis strain EXTN-1. While the percent of infected plants in the non-bacterized control plants were 95%, it was only 65% in plants pre-treated with EXTN-1. It was also demonstrated that the movement of R. solanacearum within the stem was significantly hampered when the plants were root bacterized. As EXTN-1 has no antagonistic properties against R. solanacearum, the bacterial wilt was probably suppressed by a mechanism other than antibiosis. Previously, the strain had been proven to produce an efficient elicitor for inducing systemic resistance in many crops. As the present study confirmed that EXTN-1 has the ability for reducing the pathogen spread in tomato, the strain could be effectively used as a potential biocontrol agent against bacterial wilt.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1027-1036
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• 2020

Stipa purpurea is a unique and dominant herbaceous plant species in the alpine steppe and meadows on the Qinghai-Tibet Plateau (QTP). In this work, we analyzed the composition and diversity of the culturable endophytic fungi in S. purpurea according to morphological and molecular identification. Then, we investigated the bioactivities of these fungi against plant pathogenic fungi and 1-aminocyclopropane-1-carboxylate deaminase (ACCD) deaminase activities. A total of 323 fungal isolates were first isolated from S. purpurea, and 33 fungal taxa were identified by internal transcribed spacer primers and grouped into Ascomycota. The diversity of endophytic fungi in S. purpurea was significantly higher in roots as compared to leaves. In addition, more than 40% of the endophytic fungi carried the gene encoding for the ACCD gene. The antibiosis assay demonstrated that 29, 35, 28, 37 and 34 isolates (43.9, 53.1, 42.4, 56.1, and 51.5%) were antagonistic to five plant pathogenic fungi, respectively. Our study provided the first assessment of the diversity of culture-depending endophytic fungi of S. purpurea, demonstrated the potential application of ACCD activity and antifungal activities with potential benefits to the host plant, and contributed to high biomass production and adaptation of S. purpurea to an adverse environment.

• Korea Science and Art Forum
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• v.37 no.5
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• pp.239-248
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• 2019

This study started from the need to improve one of shortcomings of synthetic PVAc adhesives - potential physical harm and environmental hazards to the workers or their users. As a matter of fact, PVAc adhesives are currently mainly used because of their convenience and economy for the production of woodcrafts. The purpose of this study was to develop natural adhesives through research on natural adhesives in step with the current increase of societal attention to environmental friendliness and rapid surge in their demand in the face of such problems. So, the study attempted research on the bases to develop natural adhesives for woodcraft, using cellulose extracts from wood - natural adhesive material. The findings of the study were as follows. Firstly, natural adhesives showed the improved effect in the field of adhesive strength, a basic physical property by 0.2 - 4 times compared with the existing materials and the study confirmed they had the similar or stable pH value. Besides, they had good reversibility, demonstrating their basic physical property as a natural adhesive for woodcraft. While, their durability to ultraviolet ray degradation also showed an excellent result value being better by 1.5 - 8.5 times than the existing materials. The study expects natural adhesives with improved and better performances compared with the existing materials could be developed, if further research on adhesive strength, antibiosis, conservative property were to continue by developing refinery technology for cellulose extracts from wood and rendering the functionality to them.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.422-428
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• 2014

Endophytic bacterial strains from root tissue of strawberry were screened for their efficacy in growth improvement and control of Phytophthora blight disease of chili pepper plant under greenhouse condition. Plants treated with the strain EP103, identified as Pseudomonas fluorescens, showed growth improvement in terms of fresh weight and root length compared to the untreated control and other endophytic strains. When challenged with Phytophthora capsici, there was significant reduction of disease in EP103 treated plants with an efficacy of 78.7%. There was no direct inhibition of the target pathogen by EP103 when tested under in vitro antibiosis assay. Analysis of differential expression of selected marker genes for induced systemic resistance (ISR) in plants treated with EP103 and challenged with P. capsici showed up-regulation of PR1 and PR10 pathogenesis-related (PR) proteins. PCR analysis showed that EP103 produced secondary metabolites such as pyoluteorin, pyrrolnitrin, hydrogen cyanide and orfamide A. This study indicated the potential of endophytic P. fluorescens strain EP103 as an efficient biocontrol agent against P. capsici in chili pepper plant.