• 생명과학회지
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• 제31권2호
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• pp.192-198
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• 2021

홍국은 동아시아 국가에서 예로부터 음식과 전통의학에 이용되어 왔다. 홍국은 특정 미생물(일반적으로 Monascus purpureus)이 쌀을 발효시켜 생산되어진다. Monascus sp.는 2차 대사과정을 통해 Monascus 색소, monacolins, γ-aminobutyric acid 등을 생산할 수 있다. Monascus 종의 대사산물인 monacolin K, γ-aminobutyric acid와 dimerumic acid 및 monascus pigments는 항산화 효과, 콜레스테롤과 혈압과 항비만 효과들이 알려져 있다. 본 연구에서는 Monascus sp.로 발효된 홍맥 에탄올 추출물의 항비만 활성을 알아보고자 지방 세포를 이용하여 실험을 실시하였다. 홍국 에탄올 추출물의 항비만 효과는 MDI로 유도된 3T3-L1 전지방세포의 Oil Red O staining과 Real time RT-PCR을 이용하여 비만 유전자 발현을 통해 확인하였다. 홍맥 추출물을 3T3-L1 전지방세포에 각각 200 ㎍/ml, 400 ㎍/ml, 800 ㎍/ml을 처리한 결과 5.04%, 12.24%, 23.52%로 지방축적을 감소시키는 것을 확인하였다. 또한, 홍맥 추출물은 3T3-L1 전지방세포로부터의 C/EBPα, SREBP-1, PPAR-γ 유전자의 발현이 농도 의존적으로 감소되는 것을 확인하였다. 본 연구를 통해 홍맥 추출물의 지방 합성 억제활성을 확인하였으며, 향후 항비만 기능성 식품소재로 활용될 수 있을 것으로 사료된다.

• Food Science and Biotechnology
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• 제15권3호
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• pp.469-473
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• 2006

Film-forming yeasts generate an undesirable yeasty flavor in fermented vegetables such as kimchi in the presence of oxygen. Antimicrobial materials including garlic oil (GO), heated garlic (HG), and allyl alcohol (AA) were investigated for use as alternative natural food preservatives to inhibit the growth of film-forming yeasts in fermented vegetables. Using the fermentation of cucumber pickles as a model system, GO, HG, and AA were effective in preventing film formation at concentrations of 0.006, 3.0, and 0.02%, respectively. The effectiveness of HG in preventing the growth of a film yeast, Hansenula anomala, was not influenced by pH, while that of potassium sorbate, a typical anti-yeast food preservative, was highly dependent on pH. All tested materials were effective when added at the beginning of fermentation due to their negligible inhibitory activity toward lactic acid bacteria.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.44-44
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• 2014

Yeasts were isolated from wild flowers of some islands and mountains such as Jeju-do, Ulleungdo, Yokjido, Seonyudo and Gyejoksan, Oseosan, Beakamsan and Deogyusan in Korea and were identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA or internal transcribed pacer(ITS) 1 and 2 including 5.8S rDNA using BLAST. Seventy two yeast strains of two hundred eighty nine species were isolated from wild flowers in islands and mountains, Korea. Among them, Cryptococcus species were isolated the most dominantly, and Metschnikowia reukaufii were also isolated thirty species, 10.3% of total strains. Twenty-three species including Cryptococcus aureus were overlapped between yeast strains of the islands and mountains. Some physiological functionality of the culture broth and cell-free extracts from two hundred eighty nine yeast strains were determined. The supernatant of Candida sp. 78-J-2 showed antioxidant activity of 22.5%, and supernatant of Metschnilowia reukaufii SY44-6 showed anti-gout xanthine oxidase inhibitory activity of 49.6% and whitening tyrosinase inhibitory activity of 38.4%, respectively.

• 대한화장품학회지
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• 제43권3호
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• pp.265-271
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• 2017

본 연구에서는 홍차 흑효모 발효물의 기능성화장품 소재로서의 가능성을 규명하고자 항산화, 미백, 항주름 효능을 확인하였다. 홍차 흑효모 발효물의 결과는 홍차 추출물, 흑효모 발효물과 비교하여 나타내었다. 항산화능 평가 결과 홍차 흑효모 발효물은 흑효모 발효물보다 뛰어난 DPPH 라디칼 소거능을 나타냈으며 홍차 추출물과는 유사한 결과를 나타냈다. 과산화수소로 유도된 세포 내 활성산소종 실험에서도 홍차 흑효모 발효물은 유의적 제거 활성을 보였다. 또한 홍차 흑효모 발효물은 tyrosinase 저해 활성을 보이며 Melan-a, B16F10 세포를 이용한 멜라닌 형성이 억제됨을 확인하였다. 항주름 효능 확인을 위해 콜라겐 분해 효소(MMP-1)를 측정한 결과 홍차 흑효모 발효물이 홍차 추출물, 흑효모 발효물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통해 홍차 흑효모 발효물은 항산화능 효능을 보이며 미백, 항주름 효능을 보아 기능성 화장품 소재로 개발될 수 있음을 확인하였다.

• Environmental Analysis Health and Toxicology
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• 제18권2호
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• pp.131-136
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• 2003

Anti-fungal materials producing bacteria were isolated from soil by bennett's agar and actinomycete isola-tion agar medium. The bacterla were identified as synonym of Actinomycetes. Based on the data obtained from its morphological and colony characteristics. The medium for production of anti-fungal materials was YEME (yeast extract 4 g, malt extract l0g, glucose 4 g, D.W 1ι, pH 7.0${\pm}$0.2). The culture conditions were 30$^{\circ}C$, 7 days and 200 rpm in shaking incubator. No. 13, No. 15 and No.28 strains were produced anti-fungal materials against fungal plant pathogens. Specially, The No. 28 strain showed a powerful biopesticide activity and broad spectrum effects of anti -fungal materials on Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

• 한국환경보건학회지
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• 제31권1호
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• pp.31-38
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• 2005

An anti-fungal material produced by actinomycetes was isolated from domestic soil. This actinomycetes was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and $25^{\circ}C$. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

• Natural Product Sciences
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• 제9권4호
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• pp.256-259
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• 2003

The pet ether extract of Caesalpinia pulcherrima, leaves was studied for its antinociceptive, anti-inflammatory and antipyretic property. The extract at doses of 50 and 200 mg/kg, p.o., significantly (p<0.05) reduced the number of writhing induced by acetic acid and inhibited the late phase (20-30 min) in formalin test in mice. The extract failed to increase the pain threshold level in tail immersion test in mice. In carrageenan induced paw edema in rats and in acetic acid induced increase in vascular permeability test in mice, the extract (50-600 mg/kg, p.o.) failed to produce any significant activity. While in cotton pellet granuloma test, the extract at doses of 200 and 600 mg/kg (p.o.) significantly (p<0.05) reduced the granuloma formation and was comparable to reference drug, dexamethasone. In ethylpheylpropiolate ear edema test 0.5 mg and 1 mg/ear application of extract significantly (p<0.05) inhibited ear edema. In yeast induced hyperthermia in rats, the extract did not produce any reduction in temperature. The results suggest that the extract acts peripherally to produce analgesic action and anti-inflammatory activity through steroidal mechanism.

• Animal Bioscience
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• 제35권6호
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• pp.858-868
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• 2022

Objective: This study was conducted to evaluate the effects of dietary yeast hydrolysate (YH) supplementation on intestinal morphology, barrier, and anti-inflammatory functions of broilers. Methods: A total of 320 one day old male broilers were randomly allocated into four groups with eight replicates of ten broilers each. The broilers were supplemented with a basal diet (the control group) or basal diets adding 50, 100, 150 mg/kg YH, respectively. This trial lasted for 42 days. The orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing levels of YH. Results: In our previous research, supplementing YH improved growth performance by enhancing body weight gain but decreased feed-to-gain ratio. In this study, compared with the control group, dietary YH addition linearly and quadratically decreased serum diamine oxidase activity (p<0.05). Additionally, supplementing YH linearly and/or quadratically decreased jejunal crypt depth (CD), tumor necrosis factor-alpha (TNF-α) concentration as well as mucin 2, interleukin-6 (IL-6), IL-1β, TNF-α, nuclear factor kappa B, and myeloid differentiation factor 88 gene expression levels (p<0.05). Whereas the jejunal villus height (VH), VH/CD, IL-10 concentration as well as zonula occludens-1 and IL-10 gene expression levels were linearly and/or quadratically increased by YH supplementation (p<0.05). Conclusion: Dietary YH supplementation improved intestinal morphology, barrier and anti-inflammatory functions while decreased intestinal permeability of broilers, which might be related with altering pertinent genes expression. This study provides evidence of YH as a promising feed additive for broilers.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.660-664
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• 2005

We have previously isolated specific RNA aptamers with high affinity against the helicase domain of hepatitis C virus (HCV) nonstructural protein 3 (NS3). The RNA aptamers competitively and efficiently inhibited the helicase activity, partially impeding HCV replicon replication in human hepatocarcinoma cells. In this study, the RNA aptamers were tested for binding to the HCV NS3 proteins in eukaryotic cells, using a yeast three-hybrid system. The aptamers were then recognized by the HCV NS3 proteins when expressed in the cells, while the antisense sequences of the aptamers were not. These results suggest that the in vitro selected RNA aptamers can also specifically bind to the target proteins in vivo. Consequently, they could be potentially utilized as anti-HCV lead compounds.

• Journal of Microbiology
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• 제40권4호
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• pp.313-318
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• 2002

To perform the prophylactic study of a vaccine derived from human papillomavirus (HPV) using Balb/c mice, we produced virus like particles consisting of HPV capsid protein L1 which has been reported to induce significant humoral and cellular immunity using various animal model systems. In order to produce HPV16 VLPs, the cDNA of L1 capsid protein in HPV type 16, obtained by polymerase chain reaction, was inserted into yeast expression vector, YEG$\alpha$-HIR525 under the control of GAL10 promoter. The transformation of YEG$\alpha$-HPV16 L1 was performed into the yeast Saccharomyces cerevisiae Y2805 by the lithium acetate method and the yeast clone expressing the highest level of L1 capsid protein of human papillomavirus type 16 was selected by Western blot analysis using anti-HPV16 L1 antibody. The purification of HPV16 VLP has been performed by the ultracentrifugation and gel-filtration methods. To validate the vaccine efficacy of the purified HPV16 VLPs and investigate the properties of HPV16 VLPs to induce humoral immunity, ELISA assay was performed. A significantly increased production of anti-HPV16 VLP antibodies was observed in sera from immunized mice. The neutralization activity of antibodies in the sera from the vaccinated mice was demonstrated by a rapid and simple assay to detect hemagglutihation inhibition activity.