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http://dx.doi.org/10.5352/JLS.2021.31.2.192

Evaluation of Lipid Accumulation's Inhibitory Activity on 3T3-L1 Cells with Red Yeast Barley Extracts  

Kwon, Gi-Seok (Division of Horticulture & Medicinal Plant, College of Life Sciences & Biotechnology, Andong National University)
Kim, Byung-Hyuk (Kyochon Research & Innovation Center, Kyochon Co., LTD.)
Lee, Jun-Hyeong (Division of Horticulture & Medicinal Plant, College of Life Sciences & Biotechnology, Andong National University)
Hwang, Hak-Soo (Kyochon Research & Innovation Center, Kyochon Co., LTD.)
Lee, Jung-Bok (Kyochon Research & Innovation Center, Kyochon Co., LTD.)
Publication Information
Journal of Life Science / v.31, no.2, 2021 , pp. 192-198 More about this Journal
Abstract
Red yeast rice has been extensively used as food and traditional medicine for thousands of years in East Asian countries. It is produced by the fermentation of a particular yeast (in general, Monascus purpureus) as rice and various cereals (barley, soybean, etc.). Monascus sp. produces many secondary metabolites during its growth, including pigments, monacolins, and γ-aminobutyric acid. Some metabolites―specifically, monacolin K, γ-aminobutyric acid, dimerumic acid, and monascus pigments―have been reported to lower cholesterol and blood pressure while showing anti-obesity effects. In this study, we investigated the anti-obesity effect of ethanol extract from red yeast barley (RYB) fermented with Monascus sp. BHN-MK 2 on 3T3-L1 cells. The anti-obesity effects of RYB extract were examined: its lipid accumulation inhibitory effect was tested by Oil Red O staining, and obesity-related mRNA expression levels were tested by real-time RT-PCR in MDI stimulated 3T3-L1 cells. The intracellular lipid content of MDI-stimulated 3T3-L1 cells decreased significantly to 5.04%, 12.24%, and 23.52% in response to 200, 400, and 800 ㎍/ml RYB, respectively. Moreovers, we evaluated that RYB extract significantly downregulated the expression of C/EBPα, SREBP-1, and PPAR-γ gene in a dose-dependent manner. As a result, red yeast barley ethanol extracts exerted the strongest anti-obesity effects. Also, the results indicate that red yeast barley could be used as a functional anti-obesity food material.
Keywords
3T3-L1; bioconversion; fermentation; Monascus sp.; red-yeast barley;
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1 Park, Y. S., Nam, G. H., Jo, K. J., Kawk, H. W., Kim, M. J., Kim, J. T., Jang, S. H., Kim, M. J. and Kim, Y. M. 2020. The anti-obesity effect of Barley sprout ethanol extract. KSBB J. 35, 72-77.   DOI
2 Patakova, P. 2012. Monascus secondary metabolites: production and biological activity. J. Ind. Microbiol. Biotechnol. 40, 169-181.   DOI
3 Patel, L., Buckels, A. C., Kinghorn, I. J., Murdock, P. R., Holbrook, J. D., Plumpton, C., Macphee, C. H. and Smith, S. A. 2003. Resistin is expressed in human macrophages and directly regulated by PPARγ activators. Biochem. Biophys. Res. Commun. 300, 472-476.   DOI
4 Srianta, I., Ristiarini, S., Nugerahani, I., Sen, S. K., Zhang, B. B., Xu, G. R. and Blanc, P. J. 2014. Recent research and development of Monascus fermentation products. Int. Food Res. J. 21, 1-12.
5 Tsukahara, M., Shinzato, N., Tamaki, Y., Namihira, T. and Matsui, T. 2009. Red yeast rice fermentation by selected Monascus sp. with deep-red color, lovastatin production but no citrinin, and effect of temperature-shift cultivation on lovastatin production. Appl. Biochem. Biotechnol. 158, 476-482.   DOI
6 Tu, T. H., Kim, C. S., Nam Goong, I. S., Nam, C. W., Kim, Y. I., Goto, T., Kawada, T., Park, T., Yoon Park, J. H., Ryoo, Z. Y., Park, J. W. Choi, H. S. and Yu, R. 2015. 4-1BBL signaling promotes cell proliferation through reprogramming of glucose metabolism in monocytes/macrophages. FEBS J. 282, 1468-1480.   DOI
7 Rosen, E. D. and Spiegelman, B. M. 2000. Molecular regulation of adipogenesis. Annu. Rev. Cell Dev. Biol. 16, 145-171.   DOI
8 Poppitt, S. D., Van Drunen, J. D., McGill, A. T., Mulvey, T. B. and Leahy, F. E. 2007. Supplementation of a high-carbohydrate breakfast with barley β-glucan improves postprandial glycaemic response for meals but not beverages. Asia Pac. J. Clin. Nutr. 16, 16-24.
9 Reynolds, C. J., Koszewski, N. J., Horst, R. L., Beitz, D. C. and Goff, J. P. 2019. Localization of the 1, 25-dihydroxyvitamin d-mediated response in the intestines of mice. J. Steroid Biochem. Mol. Biol. 186, 56-60.   DOI
10 Rosen, E. D. and MacDougald, O. A. 2006. Adipocyte differentiation from the inside out. Nat. Rev. Mol. Cell Biol. 7, 885.   DOI
11 Sargent, J. M. and Taylor, C. G. 1989. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia. Br. J. Cancer 60, 206-210.   DOI
12 Sferrazzo, G., Palmeri, R., Vanella, L., Parafati, L., Ronsisvalle, S., Biondi, A., Basile, F., Li Volti, G. and Barbagallo, I. 2019. Mangifera indica L. leaf extract induces adiponectin and regulates adipogenesis. Int. J. Mol. 20, 3211.   DOI
13 Gregoire, F. M., Smas, C. M. and Sul, H. S. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78, 783-809.   DOI
14 Shimizu, C., Kihara, M., Aoe, S., Araki, S., Ito, K., Hayashi, K., Watari, J., Sakata, Y. and Ikegami, S. 2008. Effect of high beta-glucan barley on serum cholesterol concentrations and visceral fat area in Japanese men--a randomized, double-blinded, placebo-controlled trial. Plant Foods Hum. Nutr. 63, 21-25.   DOI
15 Behall, K. M., Scholfield, D. J. and Hallfrisch, J. 2004. Diets containing barley significantly reduce lipids in mildly hypercholesterolemic men and women. Am. J. Clin. Nutr. 80, 1185-1193.   DOI
16 Chawla, A., Schwarz, E. J., Dimaculangan, D. D. and Lazar, M. A. 1994. Peroxisome proliferator-activated receptor (PPAR) gamma: adipose-predominant expression and induction early in adipocyte differentiation. Endocrinology 135, 798-800.   DOI
17 Cho, E. J., Lee, S. S. and Kwon, D. J. 2017. Manufacture technology of tofu shake added with red rice. Kor. J. Food Preserv. 24, 942-948.   DOI
18 Choi, B. H., Ahn, I. S., Kim, Y. H., Park, J. W., Lee, S. Y., Hyun, C. K. and Do, M. S. 2006. Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp. Mol. Med. 38, 599.   DOI
19 Endo, A. 1979. Monacolin K, a new hypocholesterolemic agent produced by a Monascus species. J. Antibiot. 32, 852-854.   DOI
20 Eun, C. S., Hwang, E. Y., Lee, S. O., Yang, S. A. and Yu, M. H. 2016. Anti-oxidant and anti-inflammatory activities of Barley sprout extract. J. Life Sci. 26, 537-544.   DOI
21 Jeon, T., Hwang, S. G., Hirai, S., Matsui, T., Yano, H., Kawada, T., Lim, B. O. and Park, D. K. 2004. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 75, 3195-3203.   DOI
22 Koutnikova, H. and Auwerx, J. 2001. Regulation of adipocyte differentiation. Ann. Med. 33, 556-561.   DOI
23 Lakota, K., Wei, J., Carns, M., Hinchcliff, M., Lee, J., Whitfield, M. L., Sodin-Semrl, S. and Varga, J. 2012. Levels of adiponectin, a marker for PPAR-gamma activity, correlate with skin fibrosis in systemic sclerosis: potential utility as biomarker? Arthritis Res. Ther. 14, R102.   DOI
24 Hong, H., Park, J., Lumbera, W. L. and Hwang, S. G. 2017. Monascus ruber-fermented buckwheat (Red yeast buckwheat) suppresses adipogenesis in 3T3-L1 cells. J. Med. Food 20, 352-359.   DOI
25 Huang, C. F., Li, T. C., Lin, C. C., Liu, C. S., Shih, H. C. and Lai, M. M. 2007. Efficacy of Monascus purpureus went rice on lowering lipid ratios in hypercholesterolemic patients. Eur. J. Cardiovasc. Prev. Rehabil. 14, 438-440.   DOI
26 Jeon, C. P., Lee, J. B., Choi, C. S. and Kwon, G. S. 2011. Physiological effect of Yogurt with powder two stage fermented Dioscorea batatas dence by Monascus sp. and Lactobacillus sp. Kor. J. Microbiol. 47, 151-157.
27 Kim, S. O., Kim, M. R., Hwang, K. A., Park, N. J. and Jeong, J. S. 2017. Inhibition of differentiation and anti-adipogenetic effect of the Salvia plebeia R. Br. ethanol extract in murine adipocytes, 3T3-L1 cells. J. Kor. Soc. Food Sci. Nutr. 46, 401-108.   DOI
28 McNeel, R. L. and Mersmann, H. J. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J. Nutr. Biochem. 14, 266-274.   DOI
29 Lee, K. S. and Park, G. S. 2014. Studies in the consumption and preference for sprout vegetable. J. East Asian Soc. Dietary Life 24, 896-905.
30 Ma, J., Li, Y., Ye, Q., Li, J., Hua, Y., Ju, D., Zhang, D., Cooper, R. and Chang, M. 2000. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agr. Food Chem. 48, 5220-5225.   DOI
31 Moreno, D. A., Ilic, N., Poulev, A., Brasaemle, D. L., Fried, S. K. and Raskin, I. 2003. Inhibitory effects of grape seed extract on lipases. Nutrition 19, 876-879.   DOI
32 Morrison, R. F. and Farmer, S. R. 2000. Hormonal signaling and transcriptional control of adipocyte differentiation. Nutr. J. 130, 3116S-3121S.   DOI
33 Ntambi, J. M. and Kim, Y. C. 2000. Adipocyte differentiation and gene expression. Nutr. J. 130, 3122S-3126S.   DOI
34 Ono, Y., Hattori, E., Fukaya, Y., Imai, S. and Ohizumi, Y. 2006. Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J. Ethnopharmacol. 106, 238-244.   DOI
35 Park, E. S., Lee, S. M. and Park, K. Y. 2018. In vitro anti-obesity effects of Kimchi prepared with solar salts without bittern on 3T3-L1 Adipocytes. J. Kor. Soc. Food Sci. Nutr. 47, 229-234.   DOI