Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of yeast hydrolysate supplementation on intestinal morphology, barrier, and anti-inflammatory functions of broilers

  • Wang, Ting (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Cheng, Kang (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Li, QiMing (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Wang, Tian (College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2021.08.19
  • Accepted : 2021.11.16
  • Published : 2022.06.01
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Abstract

Objective: This study was conducted to evaluate the effects of dietary yeast hydrolysate (YH) supplementation on intestinal morphology, barrier, and anti-inflammatory functions of broilers. Methods: A total of 320 one day old male broilers were randomly allocated into four groups with eight replicates of ten broilers each. The broilers were supplemented with a basal diet (the control group) or basal diets adding 50, 100, 150 mg/kg YH, respectively. This trial lasted for 42 days. The orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing levels of YH. Results: In our previous research, supplementing YH improved growth performance by enhancing body weight gain but decreased feed-to-gain ratio. In this study, compared with the control group, dietary YH addition linearly and quadratically decreased serum diamine oxidase activity (p<0.05). Additionally, supplementing YH linearly and/or quadratically decreased jejunal crypt depth (CD), tumor necrosis factor-alpha (TNF-α) concentration as well as mucin 2, interleukin-6 (IL-6), IL-1β, TNF-α, nuclear factor kappa B, and myeloid differentiation factor 88 gene expression levels (p<0.05). Whereas the jejunal villus height (VH), VH/CD, IL-10 concentration as well as zonula occludens-1 and IL-10 gene expression levels were linearly and/or quadratically increased by YH supplementation (p<0.05). Conclusion: Dietary YH supplementation improved intestinal morphology, barrier and anti-inflammatory functions while decreased intestinal permeability of broilers, which might be related with altering pertinent genes expression. This study provides evidence of YH as a promising feed additive for broilers.

Keywords

Acknowledgement

The technical assistance of colleagues in our laboratories is gratefully acknowledged.

References

  1. Zhang H, Chen YN, Chen YP, et al. Dietary pterostilbene supplementation attenuates intestinal damage and immunological stress of broiler chickens challenged with lipopolysaccharide. J Anim Sci 2020;98:skz373. https://doi.org/10.1093/jas/skz373
  2. Zhang YY, Liu YS, Li JL, et al. Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers. Asian-Australas J Anim Sci 2020;33:2008-20. https://doi.org/10.5713/ajas.19.0967
  3. Bu XY, Huang JS, Tao SQ, et al. Yeast cultures alleviate gossypol induced inflammatory response in liver tissue of Ussuri catfish (Pseudobagrus ussuriensis). Aquaculture 2020;518:734828. https://doi.org/10.1016/j.aquaculture.2019.734828
  4. Waititu SM, Yin F, Patterson R, Rodriguez-Lecompte JC, Nyachoti CM. Short-term effect of supplemental yeast extract without or with feed enzymes on growth performance, immune status and gut structure of weaned pigs challenged with Escherichia coli lipopolysaccharide. J Anim Sci Biotechnol 2016;7:64. https://doi.org/10.1186/s40104-016-0125-5
  5. Waititu SM, Yin F, Patterson R, Yitbarek A, Rodriguez-Lecompte JC, Nyachoti CM. Dietary supplementation with a nucleotide-rich yeast extract modulates gut immune response and microflora in weaned pigs in response to a sanitary challenge. Animal 2017;11:2156-64. https://doi.org/10.1017/S1751731117001276
  6. Yuan XY, Jiang GZ, Wang CC, et al. Effects of partial replacement of fish meal by yeast hydrolysate on antioxidant capability, intestinal morphology, and inflammation-related gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). Fish Physiol Biochem 2019;45:187-97. https://doi.org/10.1007/s10695-018-0552-7
  7. Zhang JY, Park JW, Kim IH. Effect of supplementation with brewer's yeast hydrolysate on growth performance, nutrients digestibility, blood profiles and meat quality in growing to finishing pigs. Asian-Australas J Anim Sci 2019;32:1565-72. https://doi.org/10.5713/ajas.18.0837
  8. Bi S, Zhang J, Qu Y, Zhou B, He X, Ni J. Yeast cell wall product enhanced intestinal IgA response and changed cecum micro-flora species after oral vaccination in chickens. Poult Sci 2020;99:6576-85. https://doi.org/10.1016/j.psj.2020.09.075
  9. Ma J, Shah AM, Shao YQ, Wang ZS, Zou HW, Kang K. Dietary supplementation of yeast cell wall improves the gastrointestinal development of weaned calves. Anim Nutr 2020;6:507-12. https://doi.org/10.1016/j.aninu.2020.06.003
  10. Ahiwe EU, Abdallh ME, Chang'A EP, et al. Influence of dietary supplementation of autolyzed whole yeast and yeast cell wall products on broiler chickens. Asian-Australas J Anim Sci 2020;33:579-87. https://doi.org/10.5713/ajas.19.0220
  11. Bilal RM, Hassan F, Saeed M, et al. Prospects of yeast based feed additives in poultry nutrition: Potential effects and applications. Indian J Anim Sci 2020;90:495-505. https://doi.org/10.56093/ijans.v90i4.104177
  12. Yousefi S, Monsef Shokri MM, Allaf Noveirian HA, Hoseinifar SH. Effects of dietary yeast cell wall on biochemical indices, serum and skin mucus immune responses, oxidative status and resistance against Aeromonas hydrophila in juvenile Persian sturgeon (Acipenser persicus). Fish Shellfish Immunol 2020;106:464-72. https://doi.org/10.1016/j.fsi.2020.08.007
  13. Wang WW, Li Z, Han QQ, Guo YM, Zhang B, D'Inca R. Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers. Br J Nutr 2016; 116:1878-88. https://doi.org/10.1017/S0007114516004116
  14. Chaiyasut C, Pengkumsri N, SivamaruthiI BS, et al. Extraction of β-glucan of Hericium erinaceus, Avena sativa L., and Saccharomyces cerevisiae and in vivo evaluation of their immunomodulatory effects. Food Sci Technol 2018;38(Suppl 1):138-46. https://doi.org/10.1590/fst.18217
  15. Duan XD, Tian G, Chen DW, et al. Mannan oligosaccharide supplementation in diets of sow and (or) their offspring improved immunity and regulated intestinal bacteria in piglet. J Anim Sci 2019;97:4548-56. https://doi.org/10.1093/jas/skz318
  16. Fu RQ, Chen DW, Tian G, et al. Effect of dietary supplementation of Bacillus coagulans or yeast hydrolysates on growth performance, antioxidant activity, cytokines and intestinal microflora of growing-finishing pigs. Anim Nutr 2019;5:366-72. https://doi.org/10.1016/j.aninu.2019.06.003
  17. Jin M, Xiong J, Zhou Q, Yuan Y, Wang X, Sun P. Dietary yeast hydrolysate and brewer's yeast supplementation could enhance growth performance, innate immunity capacity and ammonia nitrogen stress resistance ability of Pacific white shrimp (Litopenaeus vannamei). Fish Shellfish Immunol 2018;82:121-9. https://doi.org/10.1016/j.fsi.2018.08.020
  18. Timothee Andriamialinirina HJ, Irm M, Taj S, Lou JH, Jin M, Zhou Q. The effects of dietary yeast hydrolysate on growth, hematology, antioxidant enzyme activities and non-specific immunity of juvenile Nile tilapia, Oreochromis niloticus. Fish Shellfish Immunol 2020;101:168-75. https://doi.org/10.1016/j.fsi.2020.03.037
  19. Wang T, Cheng K, Yu CY, Tong YC, Yang ZB, Wang T. Effects of yeast hydrolysate on growth performance, serum parameters, carcass traits, meat quality and antioxidant status of broiler chickens. J Sci Food Agric 2022;102:575-83. https://doi.org/10.1002/jsfa.11386
  20. Wang T, Cheng K, Yu CY, et al. Effects of a yeast-derived product on growth performance, antioxidant capacity, and immune function of broilers. Poult Sci 2021;100:101343. https://doi.org/10.1016/j.psj.2021.101343
  21. National Research Council. Nutrient requirements of poultry. 9th revised. edn. Washington, DC, USA: National Academy Press; 1994.
  22. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  23. SAS Institute. Users guide. Release 8.1 ed. Cary, NC, USA: SAS Institute Inc.; 2000.
  24. Zhang C, Wang C, Chen KK, Zhao XH, Geng ZY. Effect of L-theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers. J Sci Food Agric 2020;100:1718-25. https://doi.org/10.1002/jsfa.10192
  25. Yang L, Liu G, Lian KX, et al. Dietary leonurine hydrochloride supplementation attenuates lipopolysaccharide challenge-induced intestinal inflammation and barrier dysfunction by inhibiting the NF-κB/MAPK signaling pathway in broilers. J Anim Sci 2019;97:1679-92. https://doi.org/10.1093/jas/skz078
  26. Fu RQ, Liang C, Chen DW, et al. Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function in weaned piglets. J Anim Physiol Anim Nutr 2021;105:898-907. https://doi.org/10.1111/jpn.13529
  27. Wang SQ, Zhu SL, Zhang JJ, et al. Supplementation with yeast culture improves the integrity of intestinal tight junction proteins via NOD1/NF-κB P65 pathway in weaned piglets and H2O2-challenged IPEC-J2 cells. J Funct Foods 2020;72:104058. https://doi.org/10.1016/j.jff.2020.104058
  28. Ortega A, Gil A, Sanchez- Pozo A. Exogenous nucleosides modulate expression and activity of transcription factors in Caco-2 cells. J Nutr Biochem 2011;22:595-604. https://doi.org/10.1016/j.jnutbio.2010.05.003
  29. Han FF, Fan HX, Yao M, Yang SS, Han JZ. Oral administration of yeast β-glucan ameliorates inflammation and intestinal barrier in dextran sodium sulfate- induced acute colitis. J Funct Foods 2017;35:115-26. https://doi.org/10.1016/j.jff.2017.05.036
  30. Kim K, Ehrlich A, Perng V, et al. Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli. Anim Feed Sci Technol 2019;248:114-25. https://doi.org/10.1016/j.anifeedsci.2018.12.004
  31. Kazempour F, Shargh MS, Jahanian R, Hassani S. Effect of dietary beta-glucan supplementation on growth performance, carcass characteristics and gut morphology in broiler chicks fed diets containing different theronine levels. Anim Feed Sci Technol 2017;234:186-94. https://doi.org/10.1016/j.anifeedsci.2017.07.015
  32. Agazzi A, Perricone V, Omodei Zorini FO, et al. Dietary mannan oligosaccharides modulate gut inflammatory response and improve duodenal villi height in post-weaning piglets improving feed efficiency. Animals 2020;10:1283. https://doi.org/10.3390/ani10081283
  33. Ducray HAG, Globa L, Pustovyy O, Morrison E, Vodyanoy V, Sorokulova I. Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats. J Appl Microbiol 2019;127:1192-206. https://doi.org/10.1111/jam.14361
  34. Wu C, Yang Z, Song C, et al. Effects of dietary yeast nucleotides supplementation on intestinal barrier function, intestinal microbiota, and humoral immunity in specific pathogenfree chickens. Poult Sci 2018;97:3837-46. https://doi.org/10.3382/ps/pey268
  35. Zhang Q, Chen X, Eicher SD, Ajuwon KM, Applegate TJ. Effect of threonine on secretory immune system using a chicken intestinal ex vivo model with lipopolysaccharide challenge. Poult Sci 2017;96:3043-51. https://doi.org/10.3382/ps/pex111
  36. Chen YP, Zhang H, Cheng YF, Li Y, Wen C, Zhou YM. Dietary L-threonine supplementation attenuates lipopolysaccharideinduced inflammatory responses and intestinal barrier damage of broiler chickens at an early age. Br J Nutr 2018;119:1254-62. https://doi.org/10.1017/S0007114518000740
  37. Andrianifahanana M, Moniaux N, Batra SK. Regulation of mucin expression: mechanistic aspects and implications for cancer and inflammatory diseases. Biochim Biophys Acta Rev Cancer 2006;1765:189-222. https://doi.org/10.1016/j.bbcan.2006.01.002
  38. Alizadeh A, Akbari P, Difilippo E, et al. The piglet as a model for studying dietary components in infant diets: effects of galacto-oligosaccharides on intestinal functions. Br J Nutr 2016;115:605-18. https://doi.org/10.1017/S0007114515004997
  39. Ye MB, Bak JP, An CS, et al. Dietary beta-glucan regulates the levels of inflammatory factors, inflammatory cytokines, and immunoglobulins in interleukin-10 knockout mice. J Med Food 2011;14:468-74. https://doi.org/10.1089/jmf.2010.1197
  40. Song ZH, Cheng K, Zhang LL, Wang T. Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers. J Therm Biol 2017;69:184-90. https://doi.org/10.1016/j.jtherbio.2017.07.015
  41. Bu XY, Lian XQ, Wang Y, et al. Dietary yeast culture modulates immune response related to TLR2-MyD88-NF-κB signaling pathway, antioxidant capability and disease resistance against Aeromonas hydrophila for Ussuri catfish (Pseudobagrus ussuriensis). Fish Shellfish Immunol 2019;84:711-8. https://doi.org/10.1016/j.fsi.2018.10.049
  42. Yuan K, Mendonca LGD, Hulbert LE, et al. Yeast product supplementation modulated humoral and mucosal immunity and uterine inflammatory signals in transition dairy cows. J Dairy Sci 2015;98:3236-46. https://doi.org/10.3168/jds.2014-8469
  43. Jawhara S, Habib K, Maggiotto F, et al. Modulation of intestinal inflammation by yeasts and cell wall extracts: strain dependence and unexpected anti-Inflammatory role of glucan fractions. PLoS One 2012;7:e40648. https://doi.org/10.1371/journal.pone.0040648
  44. Penney J, Lu Y, Pan B, Feng Y, Walk C, Li J. Pure yeast beta-glucan and two types of yeast cell wall extracts enhance cell migration in porcine intestine model. J Funct Foods 2019;59:129-37. https://doi.org/10.1016/j.jff.2019.05.037
  45. Choi EY, Lee SS, Hyeon JY, et al. Effects of beta-glucan on the release of nitric oxide by macrophages stimulated with lipopolysaccharide. Asian-Australas J Anim Sci 2016;29:1664-74. https://doi.org/10.5713/ajas.16.0418