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Isolation and Identification of Activated Microorganisms for Biocide Development  

Lee, Jang-Hoon (College of Environment & Safety Engineering, Hoseo University)
Kang, Byeong-Kon (College of Environment & Safety Engineering, Hoseo University)
Kwon, Hyuk-Ku (College of Environment & Safety Engineering, Hoseo University)
Jung, Joon-Oh (College of Environment & Safety Engineering, Hoseo University)
Nam, Youn-Ku (College of Environment & Safety Engineering, Hoseo University)
Publication Information
Journal of Environmental Health Sciences / v.31, no.1, 2005 , pp. 31-38 More about this Journal
Abstract
An anti-fungal material produced by actinomycetes was isolated from domestic soil. This actinomycetes was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and $25^{\circ}C$. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.
Keywords
biocide; isolation; identification; actinomycetes; Antifungal activity;
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