• 생명과학회지
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• 제22권1호
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• pp.25-30
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• 2012

TSLP 유전자는 IL-7와 유사한 새로운 조혈성 사이토카인이다. 인간의 TSLP는 상피세포, 기질세포 및 비만세포에서 만들어진다. TSLP는 류마티스관절염 환자의 윤활성 활액에서 높은 발현을 나타낸다. 이전 연구에서 우리들은 사람의 TSLP유전자에서 4개의 유전자다형성 및 한 개의 변이를 발굴하였다. 이 연구에서는, 우리들이 발굴한 TSLP유전자의 유전자다형성의 유전자형 및 대립형질의 비율을 건강한 정상인과 류마티스관절염 환자에서 비교분석하였으며, 류마티스관절염 환자에 있어서 유전자형에 따른 RF 및 anti-CCP의 정도를 비교 분석하였다. 또한, 양쪽 그룹에서 이들 유전자다형성에 의한 일배체형 비율을 비교 분석하였다. 그 결과, 류마티스관절염 환자군과 건강한 정상인 군 사이에 있어서 유전자형, 대립형질 비율뿐만 아니라 일배체형 비율에 큰 차이를 보이지 않았다. 이 결과는 TSLP유전자의 유전자다형성은 류마티스관절염 감수성에 영향을 미치지 않음을 암시한다.

• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.474-481
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• 1996

Since the commercially available rabbit anti-cyclin D3, generated from c-terminal 16 amino acid residues which are common to human and murine cyclin D3, is highly cross-reactive with many other cellular proteins of mouse, a new rabbit polyclonal anti-cyclin D3 has been raised by using murine cyclin D3 protein expressed at a high level in Escherichia coli as the immunogen. To express murine cyclin D3 protein in E. coli, the cyclin D3 cDNA fragment encoding c-terminal 236 amino acid residues obtained by polymerase chain reaction (PCR) was inserted into the NcoI/BamHI site of protein expression vector, pET 3d. Molecular mass of the cyclin D3 overexpressed in the presence of IPTG (Isopropyl $\beta$-D-thiogalactopyranoside) was approximately 26 kDa as calculated from the reading frame on the DNA sequence, and the protein was insoluble and mainly localized in the inclusion bodies that could be easily purified from the other cellular soluble proteins. When renaturation was performed following denaturation of the insoluble cyclin D3 protein in the inclusion bodies using guanidine hydrochloride, 4.4 mg of soluble form of cyclin D3 protein was produced from the transformant cultured in 100ml of LB media under the optimum conditions. Four-hundred micrograms of the soluble form of cyclin D3 protein was used for each immunization of a rabbit. When the antiserum obtained 2 weeks after tertiary immunization was applied to Western blot analysis, it was able to detect 33 kDa cyclin D3 protein in both murine lymphoma cell line BW5147.G.1.4 and human Jurkat T cells at 3,000-fold dilution with higher specificity to murine cyclin D3, demonstrating that the new rabbit polyclonal anti-murine cyclin D3 generated against c-terminal 236 amino acid residues more specifically recognizes murine cyclin D3 protein than does the commercially available rabbit polyclonal antibody raised against c-terminal 16 amino acids residues.

• 동의생리병리학회지
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• 제20권6호
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• pp.1763-1768
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• 2006

Cancer is one of the most serious health problem in modern society, and there are many studies to treat this disease. We have performed the study about analyzing papers related with anti-cancerous herbs that is used in clinical treatment recently. We found 257 papers in 'Pub med', the internet site, using the key words 'cancer' and the scientific name of anti-cancerous herbs. We also have compared the herbs' efficacy in oriental medicine with the experimental results in those papers, and thought the practical uses in clinical treatments. However, research studies that reported data on anti-cancerous herbs were wholly lacking. It is recommended that further studies be performed from the point of view of oriental medicine.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.332-339
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• 2012

The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia $Extract^{TM}$, Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer's disease effects of extract products of Magnolia officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented $A{\beta}$ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia officinalis also decreased expression of ${\beta}$-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia officinalis could show preventive effect of memorial dysfunction and $A{\beta}$ accumulation, our ethanol extract of Magnolia officinalis (BioLand LTD, Korea) could be more effective than Magnolia $Extract^{TM}$ (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating ${\beta}$-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

• 한국지반공학회논문집
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• 제26권7호
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• pp.71-80
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• 2010

지리학적 특성으로 국내는 계절 동토지역으로 겨울철에는 동상현상이 발생하고 봄철에는 지반이 해빙된다. 도로는 다양한 재료와 단면으로 구성된 구조물이기 때문에 환경성과 재료 물성뿐만 아니라 포장체 각 층의 구조적 적정성 또는 지지력을 파악하는 것이 무엇보다 중요하다. 현재 기존 동상방지층 설계법에 따르면, 동상방지층은 포장체의 구조적 적정성과는 무관하게 온도조건에 따른 동결깊이에 따라 일률적으로 결정되고 있다. 이러한 동결깊이를 포장구조설계에 적용함으로써 포장의 과다설계 우려가 있다. 따라서 본 논문에서는 노상층, 동상방지층, 보조기층의 역학적 실험을 실시하여 동상민감성을 판단하고 실규모 현장도로 모형축소 실험을 실시하였다. 동상민감성을 판단하기 위하여 토층별 온도, 동결팽창량, 부동수분 및 동결깊이를 측정하였다.

• Applied Microscopy
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• 제32권3호
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• pp.257-263
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• 2002

복합 페로브스카이트$(Li_{1/2}Pr_{1/2})TiO_3$ (LPT)의 미세구조를 X-ray diffractometry와 투과전자현미경으로 관찰하였다. LPT는 A-site 양이온 결핍에 의한 vacancy ordering을 갖고 있으며 산소 팔면체의 antiphase tilting과 inphase tilting 그리고 A-site 양이온의 anti-parallel shift 가 관찰되었다. 스피노달 분해에 의한 상변태도 관찰되었다. 측정된 NPT 재료의 유전체 특성은 ${\varepsilon}_r=84.6,\;Q\;{\Large f}_o=776\;GHz,\;{\tau}_{f}=-233.66ppm/^{\circ}C$이었다.

• Corrosion Science and Technology
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• 제3권1호
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• pp.20-25
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• 2004

In order to clarify the durability of protective coatings for maritime steel structures, various anti-corrosive organic coated steel samples were exposed for twelve years in semitropical marine environment at Miyakojima Island, Okinawa, JAPAN. Samples were various organic coated steel pipes, 4.0 m in length and 150 mm in diameter. While the bare steel pipe entirely corroded in 4.5 mm thickness in four and half years, these organic coated steel pipes exhibited protective appearances after twelve-year-exposure except for the defect in the coatings. Polyethylene (PE) lining pipe exhibited a good protective performance. Urethane painted pipe was also good but some barnacles stuck to its surface. A combination of petrolatum tape and FRP cover showed sufficient corrosion resistance for steel surface. The correlation in results between exposure and laboratory acceleration test was examined. It was found that salt spray test (SST) results corresponded to rusted area of scratched portion and that adhesion change of coating layer corresponded to the rotating immersion test result. Among the on-site measured data, volume resistivity is utilized for the index of corrosion protection performance of organic coating.

• 한국식품영양과학회지
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• 제22권4호
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• pp.374-380
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• 1993

녹차추출액을 사용하여 Sprague Dawley 휜쥐를 63일간 사육한 뒤 십이지장궤양 유발제 cysteamineㆍHCI(400mg/1kg b.w.)을 투여하면 수도물에 의해 같은 기간 사육한 대조군 (TW군) 보다 cysteamine에 대한 감수성은 유의하게 저하되어 십이지장궤양 발병율이 TW군의 50%를 나타냈다. 또한 TW군은 cysteamine투여에 의해 십이지장점막 극재 효소인 alkaline phosphatase (ALP) 활성을 상부십이지장 점막에 있어서 현저하게 저하시켰으나 GT군은 유의하게 활성이 유지되고 ALP 분자종에 있어서도 TW군과 상이한 양상을 나타내 녹차 추출액이 cysteamine 궤양에 있어서 항 궤양적 생리활성을 발휘하고 있는 사실이 밝혀졌다.

• 멤브레인
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• 제15권4호
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• pp.289-296
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• 2005

MBR 공정에서 가장 문제시되는 부분은 실제 공정상에서 막 표면에 오염이 발생하는 문제이다. 일반적으로 막 오염은 활성 슬러지들이 막 표면에 퇴적되어서 일어나며 이로 인해 심각한 투과 유량의 감소를 야기하게 된다. 본 연구에서는 막 오염 저항성이 뛰어난 나노 입자를 분리막 표면에 함침시켜 MBR 막을 제작하였으며 이 입자들은 막 표면에서 활성 슬러지들이 쉽게 달라 붙지 못하는 역할을 수행하게 된다. 즉, 뛰어난 투수량을 지닌 정밀여과막에 나노 입자를 첨가함으로서 실제 MBR 공정에서 막 오염을 저감시킴으로서 투수량을 보전할 수 있게 하였다. 이들을 이용하여 MBR 공정에서 막 오염이 휠씬 적게 일어나는 것을 확인하였으며 이를 바탕으로 현장에 적용하여 막 오염 저항성을 확인하였다.

• Biomolecules & Therapeutics
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• 제6권2호
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• pp.139-144
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• 1998

Abstract -Phyllanthus ussuriensis which belongs to Euphorbiaceae has been used as a component of Korean traditional remedy against hepatitis and jaundice. Aqueous methanolic extract of P. ussuriensis was tested for antiviral activity of hepatitis B virus (HBV) in HepG2 2.2.15 cells which were derived through transfection of cloned HBV DNA into HepG2 human hrpatoblastoma cells. P. ussuriensis extract decreased the levels of extracellular HBV virion DNA and inhibited HBV replication at concentrations ranging from 50 to 500$\mu$g/ml. Partitioning of water suspension of the extract revealed that the activity mainly resides in the EtOAc fraction. Consecutive purification of the EtOAc fraction by silica-gel column chromatography resulted in the two active anti-HBV fractions. Southern blot analysis shows that the action mechanisms or active site of the two fractions seems to be different in terms of their inhibition of HBV replication steps.