Inhibition of HBV ]Replication by the Extract of Phyllanthus ussuriensis

여우주머니(Phyllanthus ussuriensis) 추출물의 B헝 간염바이러스 증식 억제

  • Kim, Chul-Young (College of Pharmacy, Seoul National University) ;
  • Kim, Jeong-Min (LG Biotech Research Institute) ;
  • Kim, Tae-Gyun (Korea Food and Drug Administration) ;
  • Kim, Seung-Hee (Korea Food and Drug Administration) ;
  • Huh, Hoon (College of Pharmacy, Seoul National University)
  • 김철영 (서울대학교 약학대학) ;
  • 김정민 ;
  • 김태균 (식품의약품안전청) ;
  • 김승희 (식품의약품안전청) ;
  • 허훈 (서울대학교 약학대학)
  • Published : 1998.06.01


Abstract -Phyllanthus ussuriensis which belongs to Euphorbiaceae has been used as a component of Korean traditional remedy against hepatitis and jaundice. Aqueous methanolic extract of P. ussuriensis was tested for antiviral activity of hepatitis B virus (HBV) in HepG2 2.2.15 cells which were derived through transfection of cloned HBV DNA into HepG2 human hrpatoblastoma cells. P. ussuriensis extract decreased the levels of extracellular HBV virion DNA and inhibited HBV replication at concentrations ranging from 50 to 500$\mu$g/ml. Partitioning of water suspension of the extract revealed that the activity mainly resides in the EtOAc fraction. Consecutive purification of the EtOAc fraction by silica-gel column chromatography resulted in the two active anti-HBV fractions. Southern blot analysis shows that the action mechanisms or active site of the two fractions seems to be different in terms of their inhibition of HBV replication steps.