Browse > Article
http://dx.doi.org/10.4062/biomolther.2012.20.3.332

A Comparison between Extract Products of Magnolia officinalis on Memory Impairment and Amyloidogenesis in a Transgenic Mouse Model of Alzheimer's Disease  

Lee, Young-Jung (College of Pharmacy, Chungbuk National University)
Choi, Dong-Young (College of Pharmacy, Chungbuk National University)
Han, Sang-Bae (College of Pharmacy, Chungbuk National University)
Kim, Young-Hee (R&D Center, Bioland Ltd.)
Kim, Ki-Ho (R&D Center, Bioland Ltd.)
Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
Oh, Ki-Wan (College of Pharmacy, Chungbuk National University)
Hong, Jin-Tae (College of Pharmacy, Chungbuk National University)
Publication Information
Biomolecules & Therapeutics / v.20, no.3, 2012 , pp. 332-339 More about this Journal
Abstract
The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia $Extract^{TM}$, Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer's disease effects of extract products of Magnolia officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented $A{\beta}$ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia officinalis also decreased expression of ${\beta}$-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia officinalis could show preventive effect of memorial dysfunction and $A{\beta}$ accumulation, our ethanol extract of Magnolia officinalis (BioLand LTD, Korea) could be more effective than Magnolia $Extract^{TM}$ (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating ${\beta}$-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.
Keywords
Magnolia; Tg2576; Alzheimer's disease; Natural product;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Xu, Q., Yi, L. T., Pan, Y., Wang, X., Li, Y. C., Li, J. M., Wang, C. P. and Kong, L. D. (2008) Antidepressant-like effects of the mixture of honokiol and magnolol from the barks of Magnolia officinalis in stressed rodents. Prog. Neuropsychopharmacol. Biol. Psychiatry. 32, 715-725.   DOI
2 Yan, R., Bienkowski, M. J., Shuck, M. E., Miao, H., Tory, M. C., Pauley, A. M., Brashier, J. R., Stratman, N. C., Mathews, W. R., Buhl, A. E., Carter, D. B., Tomasselli, A. G., Parodi, L. A., Heinrikson, R. L. and Gurney, M. E. (1999) Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 402, 533-537.   DOI   ScienceOn
3 Zhou, H. Y., Shin, E. M., Guo, L. Y., Youn, U. J., Bae, K., Kang, S. S., Zou, L. B. and Kim, Y. S. (2008) Anti-infl ammatory activity of 4-methoxyhonokiol is a function of the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-kappaB, JNK and p38 MAPK inactivation. Eur. J. Pharmacol. 586, 340-349.   DOI   ScienceOn
4 Morris, R. (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J. Neurosci. Methods 11, 47-60.   DOI   ScienceOn
5 Munroe, M. E., Arbiser, J. L. and Bishop, G. A. (2007) Honokiol, a natural plant product, inhibits inflammatory signals and alleviates inflammatory arthritis. J. Immunol. 179, 753-763.   DOI
6 Selkoe, D. J. (1998) The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 8, 447-453.   DOI   ScienceOn
7 Song, W. Z., Cui, J. F. and Zhang, G. D. (1989) Studies on the medicinal plants of Magnoliaceae tu-hou-po of Manglietia. Yao. Xue. Xue. Bao. 24, 295-299.
8 Tachikawa, E., Takahashi, M. and Kashimoto, T. (2000) Effects of extract and ingredients isolated from Magnolia obovata thunberg on catecholamine secretion from bovine adrenal chromaffin cells. Biochem. Pharmacol. 60, 433-440.   DOI   ScienceOn
9 Tsai, T. H., Chou, C. J. and Chen, C. F. (1996) Pharmacokinetics and brain distribution of magnolol in the rat after intravenous bolus injection. J. Pharm. Pharmacol. 48, 57-59.   DOI
10 Watanabe, K., Watanabe, H., Goto, Y., Yamaguchi, M., Yamamoto, N. and Hagino, K. (1983) Pharmacological properties of magnolol and honokiol extracted from Magnolia officinalis: central depressant effects. Planta Med. 49, 103-108.   DOI   ScienceOn
11 Weeks, B. S. (2009) Formulations of dietary supplements and herbal extracts for relaxation and anxiolytic action: Relarian. Med. Sci. Monit. 15, RA256-262.
12 Westerman, M. A., Cooper-Blacketer, D., Mariash, A., Kotilinek, L., Kawarabayashi, T., Younkin, L. H., Carlson, G. A., Younkin, S. G. and Ashe, K. H. (2002) The relationship between Abeta and memory in the Tg2576 mouse model of Alzheimer's disease. J. Neurosci. 22, 1858-1867.
13 Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A. and Tang, J. (2000) Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 97, 1456-1460.   DOI
14 Lee, Y. K., Choi, I. S., Ban, J. O., Lee, H. J., Lee, U. S., Han, S. B., Jung, J. K., Kim, Y. H., Kim, K. H., Oh, K. W. and Hong, J. T. (2011c) 4-O-methylhonokiol attenuated $\beta$-amyloid-induced memory impairment through reduction of oxidative damages via inactivation of p38 MAP kinase. J. Nutr. Biochem. 22, 476-486.   DOI
15 Lee, Y. K., Yuk, D. Y., Kim, T. I., Kim, Y. H., Kim, K. T., Kim, K. H., Lee, B. J., Nam, S. Y. and Hong, J. T. (2009) Protective effect of the ethanol extract of Magnolia offi cinalis and 4-O-methylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity. J. Nat. Med. 63, 274-282.   DOI
16 Lin, S. P., Tsai, S. Y., Lee Chao, P. D., Chen, Y. C. and Hou, Y. C. (2011) Pharmacokinetics, bioavailability, and tissue distribution of magnolol following single and repeated dosing of magnolol to rats. Planta Med. 77, 1800-1805.   DOI
17 Lin, Y. R., Chen, H. H., Ko, C. H. and Chan, M. H. (2007) Effects of honokiol and magnolol on acute and infl ammatory pain models in mice. Life Sci. 81, 1071-1078.   DOI   ScienceOn
18 Lin, Y. R., Chen, H. H., Ko, C. H. and Chan, M. H. (2006) Neuroprotective activity of honokiol and magnolol in cerebellar granule cell damage. Eur. J. Pharmacol. 537, 64-69.   DOI   ScienceOn
19 Luo, L., Nong Wang, J., Kong, L. D., Jiang, Q. G. and Tan, R. X. (2000) Antidepressant effects of Banxia Houpu decoction, a traditional Chinese medicinal empirical formula. J. Ethnopharmacol. 73, 277-281.   DOI   ScienceOn
20 Matsui, N., Takahashi, K., Takeichi, M., Kuroshita, T., Noguchi, K., Yamazaki, K., Tagashira, H., Tsutsui, K., Okada, H., Kido, Y., Yasui, Y., Fukuishi, N., Fukuyama, Y. and Akagi. M. (2009) Magnolol and honokiol prevent learning and memory impairment and cholinergic deficit in SAMP8 mice. Brain Res. 1305, 108-117.   DOI
21 Lee, M., Kwon, B. M., Suk, K., McGeer, E. and McGeer, P. L. (2012a) Effects of obovatol on GSH depleted glia-mediated neurotoxicity and oxidative damage. J. Neuroimmune. Pharmacol. 7, 173-186.   DOI
22 Lee, Y. J., Choi, D. Y., Choi, I. S., Kim, K. H., Kim, Y. H., Kim, H. M., Lee, K., Cho, W. G., Jung, J. K., Han, S. B., Han, J. Y., Nam, S. Y., Yun, Y. W., Jeong, J. H., Oh, K. W. and Hong, J. T. (2012b) Inhibitory effect of 4-O-methylhonokiol on lipopolysaccharide-induced neuroinfl ammation, amyloidogenesis and memory impairment via inhibition of nuclear factor-kappaB in vitro and in vivo models. J. Neuroinfl ammation 9, 35.   DOI
23 Lee, Y. J., Choi, D. Y., Han, S. B., Kim, Y. H., Kim, K. H., Hwang, B. Y., Kang, J. K., Lee, B. J., Oh, K. W. and Hong, J. T. (2012c) Inhibitory Effect of Ethanol Extract of Magnolia offi cinalis on Memory Impairment and Amyloidogenesis in a Transgenic Mouse Model of Alzheimer's Disease via Regulating $\beta$-Secretase Activity. Phytother. Res. [Epub ahead of print]
24 Lee, Y. J., Choi, D. Y., Lee, Y. K., Lee, Y. M., Han, S. B., Kim, Y. H., Kim, K. H., Nam, S. Y., Lee, B. J., Kang, J. K., Yun, Y. W., Oh, K. W. and Hong, J. T. (2012d) 4-O-methylhonokiol prevents memory impairment in the Tg2576 transgenic mice model of Alzheimer's disease via regulation of $\beta$-secretase activity. J. Alzheimers Dis. 29, 677-690.
25 Lee, Y. J., Choi, I. S., Park, M. H., Lee, Y. M., Song, J. K., Kim, Y. H., Kim, K. H., Hwang, D. Y., Jeong, J. H., Yun, Y. P., Oh, K. W., Jung, J. K., Han, S. B. and Hong, J. T. (2011a) 4-O-Methylhonokiol attenuates memory impairment in presenilin 2 mutant mice through reduction of oxidative damage and inactivation of astrocytes and the ERK pathway. Free Radic. Biol. Med. 50, 66-77.   DOI
26 Iwasaki, K., Wang, Q., Seki, H., Satoh, K., Takeda, A., Arai, H. and Sasaki, H. (2000) The effects of the traditional chinese medicine, "Banxia Houpo Tang (Hange-Koboku To)" on the swallowing refl ex in Parkinson's disease. Phytomedicine 7, 259-263.   DOI
27 Lee, Y. J., Lee, Y. M., Lee, C. K., Jung, J. K., Han, S. B. and Hong, J. T. (2011b) Therapeutic applications of compounds in the Magnolia family. Pharmacol. Ther. 130, 157-176.   DOI
28 Hsiao, K., Chapman, P., Nilsen, S., Eckman, C., Harigaya, Y., Younkin, S., Yang, F. and Cole, G. (1996) Correlative memory deficits, Abeta elevation, and amyloid plaques in transgenic mice. Science 274, 99-102.   DOI   ScienceOn
29 Ito, K., Iida, T., Ichino, K., Tsunezuka, M., Hattori, M. and Namba, T. (1982) Obovatol and obovatal, novel biphenyl ether lignans from the leaves of Magnolia obovata Thunb. Chem. Pharm. Bull. (Tokyo) 30, 3347-3353.   DOI
30 Janus, C. and Westaway, D. (2001) Transgenic mouse models of Alzheimer's disease. Physiol. Behav. 73, 873-886.   DOI
31 Kang, J. S., Lee, K. H., Han, M. H., Lee, H., Ahn, J. M., Han, S. B., Han, G., Lee, K., Park, S. K. and Kim, H. M. (2008) Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus. Phytother. Res. 22, 883-888.   DOI
32 Kong, C. W., Tsai, K., Chin, J. H., Chan, W. L. and Hong, C. Y. (2000) Magnolol attenuates peroxidative damage and improves survival of rats with sepsis. Shock 13, 24-28.   DOI   ScienceOn
33 Kuribara, H., Stavinoha, W. B. and Maruyama, Y. (1998) Behavioural pharmacological characteristics of honokiol, an anxiolytic agent present in extracts of Magnolia bark, evaluated by an elevated plus-maze test in mice. J. Pharm. Pharmacol. 50, 819-826.   DOI   ScienceOn
34 Dikalov, S., Losik, T. and Arbiser, J. L. (2008) Honokiol is a potent scavenger of superoxide and peroxyl radicals. Biochem. Pharmacol. 76, 589-596.   DOI   ScienceOn
35 Lee, J. W., Lee, Y. K., Lee, B. J., Nam, S. Y., Lee, S. I., Kim, Y. H., Kim, K. H., Oh, K. W. and Hong, J. T. (2010) Inhibitory effect of ethanol extract of Magnolia offi cinalis and 4-O-methylhonokiol on memory impairment and neuronal toxicity induced by beta-amyloid. Pharmacol. Biochem. Behav. 95, 31-40.   DOI
36 Cui, H. S., Huang, L. S., Sok, D. E., Shin, J., Kwon, B. M., Youn, U. J. and Bae, K. (2007) Protective action of honokiol, administered orally, against oxidative stress in brain of mice challenged with NMDA. Phytomedicine 14, 696-700.   DOI
37 De Strooper, B. and Annaert, W. (2000) Proteolytic processing and cell biological functions of the amyloid precursor protein. J. Cell Sci. 113, 1857-1870.
38 Duff, K. and Suleman, F. (2004). Transgenic mouse models of Alzheimer's disease: how useful have they been for therapeutic development? Brief. Funct. Genomic. Proteomic. 3, 47-59.   DOI   ScienceOn
39 Ferri, C. P., Sousa, R., Albanese, E., Ribeiro, W. S. and Honyashiki, M. (2009) World Alzheimer Report 2009 - Executive Summary. (M. Prince and J. Jackson, Eds.), pp. 1-22. Alzheimer's Disease International, London.
40 Fujita, S. and Taira, J. (1994) Biphenyl compounds are hydroxyl radical scavengers: their effective inhibition for UV-induced mutation in Salmonella typhimurium TA102. Free Radic. Biol. Med. 17, 273-277.   DOI   ScienceOn
41 Golde, T. E., Dickson, D. and Hutton, M. (2006) Filling the gaps in the abeta cascade hypothesis of Alzheimer's disease. Curr. Alzheimer Res. 3, 421-430.   DOI
42 Chapman, P. F., White, G. L., Jones, M. W., Cooper-Blacketer, D., Marshall, V. J., Irizarry, M., Younkin, L., Good, M. A., Bliss, T. V., Hyman, B. T., Younkin, S. G. and Hsiao, K. K. (1999) Impaired synaptic plasticity and learning in aged amyloid precursor protein transgenic mice. Nat. Neurosci. 2, 271-276.   DOI
43 Hoi, C. P., Ho, Y. P., Baum, L. and Chow, A. H. (2010) Neuroprotective effect of honokiol and magnolol, compounds from Magnolia officinalis, on beta-amyloid-induced toxicity in PC12 cells. Phytother. Res. 24, 1538-1542.   DOI
44 Hou, Y. C., Chao, P. D. and Chen, S. Y. (2000) Honokiol and magnolol increased hippocampal acetylcholine release in freely-moving rats. Am. J. Chin. Med. 28, 379-384.   DOI
45 Hsiao, K. (1998) Transgenic mice expressing Alzheimer amyloid precursor proteins. Exp. Gerontol. 33, 883-889.   DOI
46 Chen, C. L., Chang, P. L., Lee, S. S., Peng, F. C., Kuo, C. H. and Chang, H. T. (2007) Analysis of magnolol and honokiol in biological fluids by capillary zone electrophoresis. J. Chromatogr. A 1142, 240-244.   DOI
47 Choi, D. Y., Lee, J. W., Lin, G., Lee, Y. K., Lee, Y. H., Choi, I. S., Han, S.B., Jung, J. K., Kim, Y. H., Kim, K. H., Oh, K. W., Hong, J. T. and Lee, M. S. (2012a) Obovatol attenuates LPS-induced memory impairments in mice via inhibition of NF-${\kappa}B$ signaling pathway. Neurochem. Int. 60, 68-77.   DOI   ScienceOn
48 Choi, D. Y., Lee, J. W., Peng, J., Lee, Y. J., Han, J. Y., Lee, Y. H., Choi, I. S., Han, S. B., Jung, J. K., Lee, W. S., Lee, S. H., Kwon, B. M., Oh, K. W. and Hong, J. T. (2012b) Obovatol improves cognitive functions in animal models for Alzheimer's disease. J. Neurochem. 120, 1048-1059.
49 Choi, J. H., Ha, J., Park, J. H., Lee, J. Y., Lee, Y. S., Park, H. J., Choi, J. W., Masuda, Y., Nakaya, K. and Lee, K. T. (2002) Costunolide triggers apoptosis in human leukemia U937 cells by depleting intracellular thiols. Jpn. J. Cancer Res. 93, 1327-1333.   DOI
50 Choi, I. S., Lee, Y. J., Choi, D. Y., Lee, Y. K., Lee, Y. H., Kim, K. H., Kim, Y. H., Jeon, Y. H., Kim, E. H., Han, S. B., Jung, J. K., Yun, Y. P., Oh, K. W., Hwang, D. Y. and Hong, J. T. (2011) 4-O-methylhonokiol attenuated memory impairment through modulation of oxidative damage of enzymes involving amyloid-$\beta$ generation and accumulation in a mouse model of Alzheimer's disease. J. Alzheimers Dis. 27, 127-141.
51 Cole, S. L. and Vassar, R. (2007) The Alzheimer's disease beta-secretase enzyme, BACE1. Mol. Neurodegener. 2, 22.   DOI   ScienceOn
52 Castellani, R. J., Zhu, X., Lee, H. G., Moreira, P. I., Perry, G. and Smith, M. A. (2007) Neuropathology and treatment of Alzheimer disease: did we lose the forest for the trees? Expert Rev. Neurother. 7, 473-485.   DOI
53 Blennow, K., de Leon, M. J. and Zetterberg, H. (2006) Alzheimer's disease. Lancet 368, 387-403.   DOI   ScienceOn