• 식품과학과 산업
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• 제50권2호
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• pp.2-11
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• 2017

소비자들의 전반적인 생활환경 향상에 따른 웰빙 라이프 추구에 따라 소비자는 맛뿐만 아니라 식품의 질적인 측면 또한 중요시하게 되었으며, 이에 따라 안전한 식품 섭취에 대한 요구가 확대되었다. 또한 가공 곡류 등의 저장 식품에서의 해충의 피해가 빈번히 일어나며, 이는 식중독 유발 등 인체에 위해를 가할 수 있다는 보고가 계속 됨에 따라 이에 대한 해결 방안이 필요하다는 목소리가 커지고 있는 실정이다. 따라서 전 세계적으로 식품에의 해충 오염 원인을 파악하고 이에 대한 해결 방안을 제시하기 위한 연구들이 지속적으로 진행되고 있으나 궁극적인 해결 방안이 제시되지 않고 있는 실정이며, 특히 방충 식품 포장재 개발 연구에 뒤따른 실질적인 상용화는 상당히 미흡한 실정이다. 따라서 식품 업계에서는 식품포장 본연의 목적을 충실히 만족하며 기능성 포장으로서 해충의 침입을 방지하는 합리적인 방충 포장재 개발기술을 발전시키는데에 총력을 기울여야 할 것이다. 더불어 앞으로 산학연의 협력하에 저장해충을 방지할 수 있는 안전성이 입증된 적합한 방충 물질 탐색에 관한 연구는 물론, 실제 산업에도 도입시킬 수 있도록 경제성을 갖춘 포장재 개발에 대한 연구가 활발히 이루어져야 할 것이다.

• 대한본초학회지
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• 제30권5호
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• pp.29-43
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• 2015

Objectives : This study was aimed to analyze the correlation between the Herbology and contemporary research results, KCD-codes.Methods : Papers were searched in OASIS and PubMed, then they were categorized. Medicine or pharmacy articles about Menthae Herba were matched with the Herbology treatment and KCD-codes. Other articles were analyzed by abstract of the papers. KCD-codes and terms were arranged by the Herbology treatment. The Degree of Herbology research (HDR) was calculated by numbers of papers, study method, citation rates.Results : There were 97 articles about Menthae Herba. Among these there were 47 medicine and pharmacy articles about Menthae Herba, and 15 articles were matched to the Herbology treatment. Studies about Headache and Wind-warmth was more active than others. Analysis of other articles showed that studies about contraceptive and anti-oxidative effect, plant growth, protective effect from insect, component extraction technics were active, too. In HDR, headache was scored by 136, Wind-warmth by 104, Eye hemorrhage by 51, Discomport in the throat by 50, Distention and fullness in the chest and hypochondrium by 15, Rubella and Measles by 0.Conclusions : 97 articles about Menthae Herba were analyzed and 15 articles were matched to the Herbology treatment. Studies about headache, wind-warmth, eye-hemorrhage were more active than others of the Herbology treatment. And studies about contraceptive and anti-oxidative effect, plant growth, protective effect from insect, component extraction technics could be a new subject of the Herbology.

• Parasites, Hosts and Diseases
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• 제59권5호
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• pp.513-518
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• 2021

The incidence of vivax malaria in Korea was reduced to a low plateau. For successful elimination of vivax malaria, socio-behavioral changes in the communities are essential. This study aimed to figure out awareness of the inhabitants on the vivax malaria endemicity. The 407 participants including vivax malaria patients and uninfected inhabitants in Gimpo- and Paju-si, Gyeonggi-do, known as high-risk areas in Korea. We used a community-based study design and non-probability sampling method using primary data. Except for the perception about the public health facilities' capability to cope with anti-malaria programs, the 2 groups of participants shared the same level of awareness about public promotional and educational measures and opinions for malaria elimination from the community. Thus, our future goals for malaria prevention and elimination are to develop more active and well-organized community-based education and evaluation programs collaborating with the community healthcare authorities and local governments.

• International Journal of Industrial Entomology and Biomaterials
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• 제24권1호
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• pp.23-27
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• 2012

Porcine circovirus type 2 (PCV2) is a single-stranded circular DNA virus associated with Postweaning multisystemic wasting syndrome (PMWS), which is considered to be an important infectious swine viral disease. PCV2 capsid protein encoded by ORF2 is a structural protein and expected as the high immunogenicity protein. In this study, we generated recombinant baculovirus containing ORF2 of PCV2 and analyzed the optimal conditions for the production of capsid protein in insect cell. Production and status of recombinant capsid protein in insect cell were confirmed by SDS-PAGE and Western blot analysis using His tag antibody and anti-PCV2 serum. The yield of recombinant capsid protein was high like as shown visible on SDS-PAGE. Optimal multiplicity of infection (MOI) and infection time of recombinant virus were determined as 5 MOI and 4 days, respectively. ORF2 is known to have N-linked glycosylation site, but we couldn't detect the glycosylation of recombinant protein in insect cells.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1583-1590
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• 2006

T4 endonuclease V (T4 endo V) [EC 3. 1. 25. 1], found in bacteriophage T4, is responsible for excision repair of damaged DNA. The enzyme possesses two activities: a cyclobutane pyrimidine dimer DNA glycosylase (CPD glycosylase) and an apyrimidic/apurinic endonuclease (AP lyase). T4 denV (414 bp cDNA) encoding T4 en do V (138 amino acid) was synthesized and expressed using either an expression vector, pTriEx-4, in E. coli or a baculovirus AcNPV vector, pBacPAK8, in insect cells. The recombinant His-Tag/T4 endo V (rHis-Tag/T4 endo V) protein expressed from bacteria was purified using one-step affinity chromatography with a HiTrap Chelating HP column and used to make rabbit anti-His-Tag/T4 endo V polyclonal antibody for detection of recombinant T4 endo V (rT4 endo V) expressed in insect cells. In the meantime, the recombinant baculovirus was obtained by cotransfection of BacPAK6 viral DNA and pBP/T4 endo V in Spodoptera frugiperda (Sf21) insect cells, and used to infect Sf21 cells to overexpress T4 endo V protein. The level of rT4 endo V protein expressed in Sf21 cells was optimized by varying the virus titers and time course of infection. The optimal expression condition was set as follows; infection of the cells at a MOI of 10 and harvest at 96 h post-infection. Under these conditions, we estimated the amount of rT4 endo V produced in the baculovirus expression vector system to be 125 mg/l. The rT4 endo V was purified to homogeneity by a rapid procedure, consisting of ion-exchange, affinity, and reversed phase chromatographies, based on FPLC. The rT4 endo V positively reacted to an antiserum made against rHis-Tag/T4 endo V and showed a residual nicking activity against CPD-containing DNA caused by UV. This is the first report to have T4 endo V expressed in an insect system to exclude the toxic effect of a bacterial expression system, retaining enzymatic activity.

• 생명과학회지
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• 제31권5호
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• pp.475-480
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• 2021

국내에서 2016년 흰점박이꽃무지 등 일부 곤충이 식품원료로 등록되면서 곤충사육 농가가 폭발적으로 증가하고 있다. 하지만 곤충사육 농가가 보유하고 있는 사육기술, 병해충 관리기술이 농가별로 차이가 있고, 대량으로 곤충을 사육하면서 높은 사육밀도, 병충해 감염 등으로 인해 곤충이 대량 폐사하게 됨으로써 곤충사육 농가에 심각한 경제적 손실을 입고 있다. 이에 본 연구에서는 다양한 영양성분을 함유할 뿐만 아니라 항균, 항염증 등의 효과를 가진 새싹보리를 흰점박이꽃무지 유충의 주 먹이원인 발효톱밥에 첨가하여 주 먹이원 첨가제로써 적용 가능성 및 곤충병원성 세균에 대한 항균 효과를 확인하고자 하였다. 흰점박이꽃무지 유충의 주 먹이원에 5%와 10% 새싹보리를 첨가했을 때 유충의 증체율이 19.2%와 23.1%로 높았고, 사육 기간이 대조군 대비 단축된 것으로 조사되었다. 또한 새싹보리는 곤충병원성 세균 Serratia marcescens의 성장을 저해하고, 세균에 감염된 흰점박이꽃무지 유충의 생존율과 증체율이 건강한 유충의 수준으로 회복되는 경향을 보였다. 뿐만 아니라 새싹보리를 첨가한 먹이원으로 사육한 유충의 영양성분 분석 결과, 조단백질, 조지방, 구리, 아연, 칼륨 등 미네랄 및 아미노산 함량이 대조군보다 높게 나타났다. 결론적으로 흰점박이꽃무지 유충의 주 먹이원에 새싹보리를 첨가한다면 단기간에 대량생산이 가능하고, 곤충병원성 세균 감염에 의한 폐사를 효율적으로 예방할 수 있어 안정적인 곤충 생산이 가능할 것으로 판단된다.

• 한국버섯학회지
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• 제20권1호
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• pp.7-12
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• 2022

The ascomycete fungus Cordyceps militaris infects lepidopteran insect pupae, forming characteristic fruiting bodies called "Dong Chung Ha Cho" in Korean. They have been used as medicines owing to their anti-allergic, anti-inflammatory, and immune-enhancing effects. This fungus can be grown on the geminated soybeans Rhynchosia nulubilis, which also contains several novel isoflavones. We performed a comparative transcriptome analysis to determine core gene sets or pathways contributing to biologically active products such as isoflavone. Initially, we sequenced 2-week-old fungal cultures on different soybean agar media, where different amounts of water agar were implemented to show different surface topology. We selected 830 upregulated and 188 downregulated genes by comparing linear models of the samples (two-fold change threshold). Gene ontology analysis identified that the "IMP biosynthesis" term was significantly found in the upregulated gene sets. The pathway is involved in the synthesis of cordycepin, the reference chemical for C. militaris. This finding in the transcriptome data is consistent with the previous observation: increased cordycepin concentrations in the C. militaris cultured on germinated soybean.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.315-319
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• 2003

A novel method is developed to yield virus titers in 10 h, is easy to .perform using 96-well plates, and applicable to both any Autographa californica nucleopolyhyderovirus (AcNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV)-based recombinant baculovirus. This assay uses an antibody to a DNA-binding protein to detect the infected cells via immune-staining. The titer is determined by counting foci produced due to infection of virus under a fluorescent microscopy. The required incubation period was shortened considerably because infected cells expressed viral antigens at the post infection time of 4 h. Therefore, 10 hours were enough to estimate the virus titer including virus infection time, insect cell culture, and estimation of virus titer.

• Journal of Microbiology and Biotechnology
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• 제2권2호
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• pp.122-128
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• 1992

Post-translational modifications of the nucleocapsid protein of bovine coronavirus (Quebec strain) were investigated. Coronavirions were radiolabelled in vivo with inorganic $[^{32}P]$orthophosphate and analysed by SDS-PAGE, followed by autoradiography. A single polypeptide with a migration rate of 55 KDa was identified by metabolic phosphate labelling, demonstrating that the nucleocapsid protein of bovine coronavirus was a phosphoprotein. A gene encoding the nucleocapsid protein was inserted immediately downstream from the polyhedrin promoter of Autographa californica nuclear polyhedrosis baculovirus. Spodoptera frugiperda cells infected with this recombinant baculovirus synthesized a 55 KDa polypeptide, as demonstrated by immunoprecipitation with anti-nucleocapsid monoclonal antibody. The recombinant nucleocapsid protein synthesized in Spodoptera cells could also be labelled by $[^{32}P]$orthophosphate. Phosphoamino acid analysis showed that both serine and threonine residues were phosphorylated in authentic, as well as in recombinant nucleocapsid proteins, with a relative phosphorylation ratio of 7:3. Our studies demonstrated that the nucleocapsid protein of bovine coronavirus was a serine and threonine-phosphorylated protein and that Spodoptera insect cells were able to properly phosphorylate the relevant foreign proteins.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1994년도 하계학술대회 논문집 C
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• pp.1643-1645
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• 1994

It has been reported frequently that damage to underground electric power cable was brought about, due to attack by Insect such as termite (white-ant) and by rodent such as rat, in tropical and semitropical areas. Among these cable damages, we are likely to describe the damage to cable by termites as causing the severe trouble in cable operation and to solve this trouble with termite-proof method regarded as the most effective and economical one. This report covers our experiences of manufacturing the cable for anti-termite purpose and its intrinsic properties in cable operation.