한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Rapid determination of baculovirus titers an antibody-based assay

  • Kwon, M.S. (Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University) ;
  • Dojimal, T. (Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University) ;
  • Park, Enoch-Y. (Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University)
  • Published : 2003.04.11
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Abstract

A novel method is developed to yield virus titers in 10 h, is easy to .perform using 96-well plates, and applicable to both any Autographa californica nucleopolyhyderovirus (AcNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV)-based recombinant baculovirus. This assay uses an antibody to a DNA-binding protein to detect the infected cells via immune-staining. The titer is determined by counting foci produced due to infection of virus under a fluorescent microscopy. The required incubation period was shortened considerably because infected cells expressed viral antigens at the post infection time of 4 h. Therefore, 10 hours were enough to estimate the virus titer including virus infection time, insect cell culture, and estimation of virus titer.

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