• 한국식품저장유통학회지
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• 제12권3호
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• pp.267-274
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• 2005

천식과 관련하여 산화스트레스 (Oxidant stress)는 그 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 농산물과 한약재를 이용하여 항산화 물질을 찾고자 하였으며, 시간과 비용의 단축을 위하여 HTS인 throughput screening)을 이용을 하였다. 항산화 실험과 관련하여서 DPPH(1,1-diphenyl-2-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), HO(hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 시행하였다. 이후 $H_{2}O_2$에 의한 산화스트레스를 이용한 세포사멸을 유도하여 세포생존을 확인해 보았다. 실험결과 해바라기씨(Helianthus annuus), 신선초(Angelica utilis Makino), 시금치(Rehmannia glutinosa Libo) 등이 활성이 높게 나타났다. 본 연구는 여기에서 나온 hit를 이용하여 동물모델 실험을 진행하고자 한다.

• 한국통신학회논문지
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• 제35권3B호
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• pp.440-452
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• 2010

소프트웨어 제품의 평가를 위해 국제표준 ISO/IEC 품질인증 시스템을 기반으로 국내 외 기관 및 연구소에서 품질에 대한 많은 방법론이 연구 및 적용되고 있으나, 복잡한 차원의 특수한 성질을 지닌 안티바이러스 소프트웨어를 평가하기에는 많은 문제를 동반한다. 따라서 본 논문에서는 적정 수준 이상의 요건을 갖춘 안티바이러스 소프트웨어의 품질평가 방법론을 마련하고자 평가항목 도출을 위한 프로세스와 정량화 방안을 정립하였으며 각 요인간의 상대적 중요도를 분석함으로써 가중치 정보를 객관화하였다. 정의된 정보(평가 항목, 가중치)를 기반으로 포털 사이트에서 수집한 공개용 안티바이러스 소프트웨어 70종에 대하여 리얼 테스트 환경에서 품질평가를 수행하였으며, 사용자들의 오랜 시간동안의 경험을 이용한 실증분석 결과 본 논문에서 정의한 평가항목과 가중치에 대한 정당성을 마련할 수 있었다.

• Toxicological Research
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• 제16권2호
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• pp.163-172
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• 2000

Recently, there is a worldwide concern that a great number of man-made chemicals have a hormone-like action both in humans and in animals. DECD is developing screening programs using validated test systems to determine whether certain substances may have an effect in humans. In the present study. the establishment oj repeated-dose toxicity test method was tried. Flutamide. an anti-androgenic agent. was administered by gavage to Sprague-Dawley rats for 28 days at dose levels of 0. 0.5. 3 and 18 mg/kg body weight (10-15 rats/sex/group) to examine the effects on general findings. especially reproductive and endocrine parameters. Clinical signs. body weights, food consumption, and sexual cycle were checked and measured. For the gross and microscopic examinations. 10 rats/sex/group were sacrificed at the end of dosing period and the remaining animals of control and high dose groups (5 each) were sacrificed after 14 days recovery. Examinations for hematology and clinical chemistry were carried out at necropsy. There were no treatment-related changes in clinical signs. body weights, food consumption. gross necropsy. hematology and clinical chemistry at all doses of both sexes. The period and regularity of sexual cycle were not adversely affected at all doses by the test agent. At 18 mg/kg. both decreased weights of prostate, seminal vesicle and epididymis in males and increased weights of spleen and thymus in females were observed. In addition, decreased number of spermatids and sperms. increased serum testosterone concentration and increased incidence (100%) of interstitial cell hyperplasia were seen in males. At 18 mg/kg of the recovery group. decreased prostate weight. reduced sperm count and increased incidence (20%) of interstitial cell hyperplasia in males and increased thymus weight in females were observed. At 3 mg/kg. reduced sperm count was found. There were no adverse effects on parameters examined at 0.5 mg/kg of both sexes. The results suggested that the potential target organs of flutamide may be accessory sexual glands including testes for males and spleen and thymus for females. Taken together. this test method was found to be a useful screening test system for endocrine disrupting chemicals.

• 복식
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• 제52권5호
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• pp.173-186
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• 2002

Street Style, occurred in British and America, has been expressed the character of the new generation by repeating developments and changes. Nowadays, pop music reflects the phase of society. and simultaneously it has influence on from culture to society. Rock Music was rooted in Country 8l western of America. Upon Country & Western, Afro-Americans Rhythm & Blues was added, and that was the birth of Rockabilly. Rockabilly developed to Rock'n 'roll and it started to change to various forms of Rock since 1950s. As the commercial impact and the breakthrough of teenagers emotion, the rock culture comprised the base of the youth culture. However. it formed the anti-establishment culture against the established value, accepting working class subculture. The teenager culture was affected by the Rock culture, also found relief in the opulence provided by the established generation, imitating it as it was. Simultaneously, it had a contrary aspect as anti-establishment form under the banner of revolt against languor in richness. The youth culture created street style that was escaped from high fashion, every time Rock music had changes. Since Street style was based on resistance of established culture and it fully refused vogue, it was indifferent from high fashion. The results of this study were as following. First, every time Rock music had changes, the new youth culture was concomitant with, the youth culture created street style. Secondly, rockahibilly style was characterized as diamond shaped design, embroidery, extra wide shirt collar, vivid contrast color . Rockers style was represented as metal studs, beads, denim, leather jacket, boots. Fusion of hippies style and Psychedelic, long hair, beads. worn denim were elements of headbangers style. Punks style was characterized as ripped T-shirt. rooster hair, over decorated jacket, short skirt. net or plastic T-shirt. Lastly, the firm relation between popular art and fashion was proved by examining the history of Rock Music and Street Style.

• 한국한의학연구원논문집
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• 제14권3호
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• pp.41-47
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• 2008

Objective : The purpose of this report was to provide the information about activity and safety of Ssanghwa-tang by analyzing domestic papers and theses about Ssanghwa-tang. Methods : Domestic papers and theses related to Ssanghwa-tang were reviewed and analyzed. These papers were then classified by year, experimental method, and activity subject. Results : The following result s were obtained in this study. 1. The study of Ssanghwa-tang started from 1981 and continued steadily 1990s and it was decreased 2000s. 2. The paper of Ssanghwatang was entire domestic paper, 20 volumes. 3. In classified by experimental method and animal of Ssanghwa-tang paper, more than a half study used rat in vivo experiment. Furthermore clinical experiments were performed about anti-fatigue effect of Ssanghwa-tang and Ssanghwatang was proved a safety herbal medicine in acute toxicity experiment. 4. Decursin, glycyrrhizin, paeoniflorin were determined in Ssanghwa-tang by using HPLC analysis. 5. As these studies were classified by subject, papers related to anti-fatigue effect were most abundant by 7 papers. Besides there were several papers related to liver funtion, anti-inflammatory, alleviation, smooth muscle relaxation effect and so on. 6. According to experimental data, it is supported that Ssanghwa-tang was safety herbal medicine and it has a several activity about anti-fatigue, anti-inflammatory, alleviation, smooth muscle relaxation. Conclusion : Many study of basic science experiments and clinical experiments were performed and reported on Ssanghwa-tang in domestic journal. Ssanghwa-tang is being used in various ways associating with anti-fatigue, anti-inflammatory, alleviation and smooth muscle relaxation. furthermore Ssanghwatang was proved a safety herbal medicine in acute toxicity experiment. However, studies on randomized controlled trials of Ssanghwatang need to be completed at the clinical level.

• 한국콘텐츠학회논문지
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• 제21권12호
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• pp.408-422
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• 2021

본 연구는 항노화 상품의 신뢰형성요인을 발굴하고 측정변수를 개발하여 결과변수인 신뢰와 구매의도 간의 영향관계를 실증적으로 검증하였다. 기존의 신뢰형성요인인 지각된 품질, 지각된 평판, 브랜드 인지도, 맞춤화에 더하여 새롭게 발굴된 요인은 '공인된 인증'이다. 우리나라의 항노화산업은 소비자 건강에 직접 영향을 미치는 산업이기 때문에 기존의 신뢰형성요인이 소비자 신뢰 구축에 한계가 있는 것으로 판단하여 새로운 척도를 발굴하였다. 본 연구는 항노화 상품 소비자를 대상으로 설문조사를 실시하여 문항의 견실성과 관계성을 분석하였다. 분석결과, 항노화 상품 신뢰형성요인 가운데 지각된 품질, 브랜드 인지도, 맞춤화, 공인된 인증이 신뢰에 정(+)의 영향을 미치는 것으로 나타났고, 지각된 평판은 신뢰에 부(-)의 영향을 미치는 것으로 나타났다. 후행변수인 신뢰와 구매의도 간에는 정(+)의 영향을 미치는 것으로 나타났다. 본 연구는 전략적으로 실효성 높은 유의미한 신뢰형성요인 발굴과 측정문항을 개발하여 실증적, 실무적으로 검증함으로써 신뢰형성요인과 신뢰와의 유의미한 관계를 입증하였다. 동시에 항노화 산업의 마케팅에 중요한 신뢰형성 핵심요인 발굴을 통해 향후의 항노화 상품관련 마케팅전략 수립에 본 연구가 상당부분 기여할 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2943-2948
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• 2012

Arsenic exposure is a serious health hazard worldwide. We have previously established that it may result in immune suppression by upregulating Th2 cytokines while downregulating Th1 cytokines and causing lymphocytic death. Treatment modalities for arsenic poisoning have mainly been restricted to the use of chelating agents in the past. Only recently have combination therapies using a chelating agent in conjunction with other compounds such as anti-oxidants, micronutrients and various plant products, been introduced. In the present study, we used T11TS, a novel immune potentiating glycopeptide alone and in combination with the sulfhydryl-containing chelator, mono-iso-amyl-dimarcaptosuccinic acid (MiADMSA) as a therapeutic regimen to combat arsenic toxicity in a mouse model. Results indicated that Th1 cytokines such as TNF-${\alpha}$, $IFN{\gamma}$, IL12 and the Th2 cytokines such as IL4, IL6, IL10 which were respectively downregulated and upregulated following arsenic induction were more efficiently restored to their near normal levels by T11TS alone in comparison with the combined regimen. Similar results were obtained with the apoptotic proteins studied, FasL, BAX, BCL2 and the caspases 3, 8 and 9, where again T11TS proved more potent than in combination with MiADMSA in preventing lymphocyte death. The results thus indicate that T11TS alone is more efficient in immune re-establishment after arsenic exposureas compared to combination therapy with T11TS+MiADMSA.

• 한국인터넷방송통신학회논문지
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• 제20권4호
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• pp.165-170
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• 2020

빠르게 성장하는 신재생 ESS 부품 소재 시장 선점과 급변하는 세계 신재생 ESS 시장에서 국내 중소기업이 대응하기에는 한계가 있으며 이를 극복하기 위한 대·중소기업 협력 기반구축과 중·장기적으로 신재생에너지 산업육성을 촉진할 수 있는 신재생 ESS 융복합 사업화 지원센터를 구축할 필요성이 커지고 있다. 따라서 신재생 ESS 융복합 전문평가센터 기반구축을 통해 신재생 ESS 제품에 대한 성능평가 및 표준화를 통해 제품의 신뢰성을 높이고, 신재생 ESS의 신뢰성 확보를 통해 국내 보급사업 및 수출 활성화를 촉진할 수 있다. 또한, 국내 연구기관, 제조업체의 개발 역량 제고 및 경쟁력 강화를 지원하고 Total-test 시험평가 시스템에 따른 시험비용 및 시험 기간의 단축을 실현하여 국내 기업이 해외 시험기관을 이용할 때 발생할 수 있는 국내 기술의 유출방지 효과를 기대할 수 있다. 따라서 본 논문에서는 이러한 성능 평가 및 실증을 위해 표준화를 정립할 수 있는 선진국 수준 이상의 전문평가센터 구축을 연구하고자 한다.

• 한국동물생명공학회지
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• 제35권1호
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• pp.21-27
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• 2020

Somatic cell nuclear transfer derived embryonic stem cells (NT-ESCs) have significant advantages in various fields such as genetics, embryology, stem cell science, and regenerative medicine. However, the poor establishment of NT-ESCs hinders various research. Here, we applied fasudil, a Rho-associated kinase (ROCK) inhibitor, to develop somatic cell nuclear transfer (SCNT) embryos and establish NT-ESCs. In the study, MII oocytes were isolated from female B6D2F1 mice and performed SCNT with mouse embryonic fibroblasts (MEFs). The reconstructed NT-oocytes were activated artificially, and cultured to blastocysts in KSOM supplemented with 10 μM fasudil. Further, the blastocysts were seeded on inactivated MEFs in embryonic stem cell medium supplemented with 10 μM fasudil. A total of 26% of embryos formed into blastocysts in the fasudil treated group, while this ratio was 44% in the fasudil free control group. On the other hand, 30% of blastocysts were established NT-ESCs after exposure of fasudil, which was significantly higher than the control group (10%). The results suggest that fasudil reduced blastocyst development after SCNT due to inhibition of 2 cell cleavage while improved the establishment of NT-ESCs through the anti-apoptotic pathway.

• 약학회지
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• 제54권6호
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• pp.488-493
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• 2010

Therapeutic manipulation of angiogenesis, the formation of new vascular sprouts from existing capillaries, is one of the promising strategies for treatment of human diseases such as cancer, arthritis, and cardiovascular disorder. In the present study, we examined the effects and molecular mechanism of tissue inhibitor of metalloproteinases-2 (TIMP-2) and endostatin on fibroblast growth factor-2 (FGF-2)-stimulated endothelial cell proliferation, migration and adhesion in vitro, and angiogenesis in vivo. TIMP-2 and endostatin showed potent anti-angiogenic activity in vitro and in vivo. These effects appear to be mediated through different angiogenic signaling pathways. Collectively, our findings demonstrate that TIMP-2 and endostatin strongly inhibit FGF-2-induced angiogenic responses, and the establishment of fast and reproducible evaluation system in vitro and in vivo for the development of anti-angiogenic biomaterials and therapeutics.