• 제목/요약/키워드: animal test

검색결과 2,415건 처리시간 0.029초

Evaluation of hemagglutination inhibition test for canine respiratory coronavirus antibodies and seroprevalence in Korean dogs

  • Lee-Sang Hyeon;Dong-Kun Yang;Yu-Ri ,Park;Hye Jeong Lee;Ha-Hyun Kim;Bang-Hun Hyun
    • 대한수의학회지
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    • 제63권4호
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    • pp.37.1-37.7
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    • 2023
  • Canine respiratory coronavirus (CRCoV) is a significant pathogen that causes respiratory diseases in dogs, collectively known as a canine infectious respiratory disease. The virus is highly contagious and exhibits high seroprevalence worldwide. Currently, bovine coronavirus (BCoV) enzyme-linked immunosorbent assay (ELISA) kits are used to detect CRCoV antibodies. However, BCoV-ELISA kits cannot differentiate between infections caused by BCoV and those caused by CRCoV. In this study, we evaluated the hemagglutination inhibition (HI) test for CRCoV by comparing it with the virus neutralization (VN) test. Subsequently, we evaluated the seroprevalence of CRCoV in 383 dog serum samples collected from South Korea utilizing the HI test. The HI test for CRCoV showed a strong correlation with the VN test (R = 0.83, p < 0.001). The analysis of seroprevalence revealed that 52.2% (95% confidence interval [CI], 47.2%-57.1%) of the Korean dog serum samples were positive. The seroprevalence exhibited varied with age, with a positivity rate of 43.9% in dogs under 1 year of age and 66.7% in dogs aged 3 to 5 years (odds ratio, 2.54; 95% CI, 1.43-4.59). In conclusion, the HI test to monitor CRCoV antibody proved to be closely related to the VN test. Furthermore, over half of the dogs in Korea tested positive for CRCoV antibodies. These findings contribute to a better understanding of the sero-epidemiology of CRCoV.

Effect of Fomes fomentarius Extract in Muscle Atrophy Rat Model

  • Gil-Hyun Lee;Kyung-Yae Hyun
    • 대한의생명과학회지
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    • 제29권4호
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    • pp.296-301
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    • 2023
  • This study was conducted to induce muscle loss using dexametasone and then use the extract to determine its effectiveness in a muscle loss animal model. Animal experimental groups were divided by five groups. Changes in the weight of the animals were measured for a total of 5 weeks. After animal sacrifice, muscle mass was measured, and animal behavior evaluation was conducted using grip strength test and pole test. The expression levels of MAFbx protein was measured using muscle samples. Oral administration of Fomes fomentarius extract was effective in suppressing muscle atropy and increasing muscle, which was confirmed through animal behavior evaluation and muscle-related protein expression.

Comparison of the fit of automatic milking system and test-day records with the use of lactation curves

  • Sitkowska, B.;Kolenda, M.;Piwczynski, D.
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권3호
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    • pp.408-415
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    • 2020
  • Objective: The aim of the paper was to compare the fit of data derived from daily automatic milking systems (AMS) and monthly test-day records with the use of lactation curves; data was analysed separately for primiparas and multiparas. Methods: The study was carried out on three Polish Holstein-Friesians (PHF) dairy herds. The farms were equipped with an automatic milking system which provided information on milking performance throughout lactation. Once a month cows were also subjected to test-day milkings (method A4). Most studies described in the literature are based on test-day data; therefore, we aimed to compare models based on both test-day and AMS data to determine which mathematical model (Wood or Wilmink) would be the better fit. Results: Results show that lactation curves constructed from data derived from the AMS were better adjusted to the actual milk yield (MY) data regardless of the lactation number and model. Also, we found that the Wilmink model may be a better fit for modelling the lactation curve of PHF cows milked by an AMS as it had the lowest values of Akaike information criterion, Bayesian information criterion, mean square error, the highest coefficient of determination values, and was more accurate in estimating MY than the Wood model. Although both models underestimated peak MY, mean, and total MY, the Wilmink model was closer to the real values. Conclusion: Models of lactation curves may have an economic impact and may be helpful in terms of herd management and decision-making as they assist in forecasting MY at any moment of lactation. Also, data obtained from modelling can help with monitoring milk performance of each cow, diet planning, as well as monitoring the health of the cow.

구제역 백신의 국가출하승인검정을 통한 품질 평가 (Evaluation for foot-and-mouth disease vaccines in South Korea by the test for national lot release)

  • 김문현;서민구;이향심;김지연;김지예;김용상;김연희
    • 한국동물위생학회지
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    • 제42권4호
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    • pp.285-288
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    • 2019
  • Foot-and-mouth disease (FMD) causes economic problems in livestock industry because of fast spread and inducing low productivity. FMD outbreaks occurred in South Korea over the period from 2000 to 2019. Vaccination is the most practical and effective means of controlling or preventing these outbreaks, and a national vaccination policy has been in place for all FMD-susceptible animals since 2010. To prevent and control of FMD, South Korea has been using vaccines imported from the United Kingdom, Argentina, and Russia. The Animal and Plant Quarantine Agency of South Korea oversees continuous quality control of imported FMD vaccines. FMD vaccines were evaluated characteristics, sterility, pH, inactivation, safety, potency test by Korean FMD vaccine standard assay (Test for National Lot Release). The 6 company vaccines (A~F) were used Test for National Lot Release by each method. We evaluated quality of each FMD vaccine from 2015 to 2019. All batch of vaccine showed good quality control and were passed the Test for National Lot Release. The serotypes of vaccine are increasingly changing to multiple vaccine because the FMD was outbreak by various serotype virus in South Korea. Furthermore, this data may be useful as a basis for ensuring the quality of FMD vaccines and for base data to manage them. Additional study is required to simple approach for rapid evaluation of quality and antigen content identification in vaccines.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja;Joh, Seong-Joon;Choi, Kang-Seuk;Kim, Aeran;Seo, Min-Goo;Song, Jae-Young;Yun, Seon-Jong
    • 대한수의학회지
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    • 제56권3호
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    • pp.189-192
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    • 2016
  • The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

국내 가축에서 브루셀라병에 대한 혈청학적 모니터링 (Serological monitoring on brucellosis in livestock of Korea)

  • 성소라;김지연;허문;이기찬;강성일;이향근;조효림;이진주;정석찬
    • 대한수의학회지
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    • 제54권4호
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    • pp.197-201
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    • 2014
  • In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

Impact of environmental factors on milk β-hydroxybutyric acid and acetone levels in Holstein cattle associated with production traits

  • Ranaraja, Umanthi;Cho, Kwang Hyun;Park, Mi Na;Choi, Tae Jung;Kim, Si Dong;Lee, Jisu;Kim, Hyun Seong;Do, Chang Hee
    • 농업과학연구
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    • 제43권3호
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    • pp.394-400
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    • 2016
  • The objective of this study was to estimate the environmental factors affecting milk ${\beta}$-hydroxybutyric acid (BHBA) and acetone (Ac) concentrations in Holstein cattle. A total of 264,221 test-day records collected from the Korea Animal Improvement Association (KAIA) during the period of 2012 to 2014 were used in this study. Analysis of variance (ANOVA) was performed to determine the factors significantly affecting ketone body concentrations. Parameters considered in the model were season of test, season of calving, parity, lactation stage, and milk collecting time (AM and PM). According to the ANOVA, the $R^2$ for milk BHBA and Ac were 0.5226 and 0.4961, respectively. 'Season of test' showed a considerable influence on ketone body concentration. Least square (LS) means for milk BHBA concentrations was the lowest ($39.04{\mu}M$) in winter while it increased up to $62.91{\mu}M$ in summer. But Ac concentration did not significantly change along with 'season of test'. The means of milk BHBA and Ac concentrations were high at first lactation stage, low around second lactation stage, and then gradually increased. Cows milked in the morning had lower mean BHBA and Ac concentrations ($48.49{\mu}M$ and $121.69{\mu}M$, respectively) in comparison to those milked in the evening ($53.46{\mu}M$ and $130.42{\mu}M$, respectively). The LS means of BHBA and Ac slightly increased over parities. These results suggest that proper maintenance of milk collection, herd management programs, and evaluation of ketone body levels in milk should be considered for the efficient management of resistance to ketosis.

Effects of the multimodal intervention program including animal-assisted therapy on depression and self-esteem among university students

  • Kil, Taeyoung
    • Journal of Animal Science and Technology
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    • 제63권6호
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    • pp.1443-1452
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    • 2021
  • This study aimed to investigate the effects of the multimodal group intervention that combined animal-assisted therapy (AAT) and integrated play therapy (IPT) on depression and self-esteem in undergraduate university students. The subjects were 40 students attending animal-related and social welfare departments of universities located in a metropolitan city. The multimodal intervention program was conducted for eight sessions (twice a week, 60 min each) in the experimental group. Data analysis was conducted using the independent sample t-test, ANCOVA, and paired sample t-test for pre- and post-test. Therefore, the multimodal intervention program applying AAT and IPT showed positive effects on depression and self-esteem in university students. Based on these results, this study proposed the operation of multidisciplinary education and practical and policy utilization methods to reduce depression among university students and help improve their self-esteem.

장기폭기시킨 축사뇨오수의 벼재배 시비효과 (The Fertilizing Effect of the Long-Time Aerated Animal Wastewater for the Paddy Rice)

  • 오인환
    • 한국축산시설환경학회지
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    • 제3권1호
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    • pp.35-42
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    • 1997
  • The purpose of this study is to investigate the effect of the biologically treated animal wastewater as a liquid fertilizer in the paddy rice. The treated water has content of the ammonia nitrogen 24mg/I and nitrate 40 mg/I on an average in the first year. A trace of nitrite has been shown. The phosphate content was 92 mg/I on an average. The amount of yielded rice was 365kg/10a for test 1 (no fertilizer), 388dg/10a for test 2(chemical fertilizer), 526kg/10a for test 3 (treated water) respectively. The treated water showed 35% more in production than the chemical fertilizer. The treated water has content of the ammonia nitrogen 40mg/I and nitrate 67mg/I on an average in the second year. A trace of nitrite has been shown. The phosphate content was 57mg/I on an average. The amount of yielded rice was 402kg/10a for test 1, 505kg/10a for test 2, 607kg/10a for test 3 respectively. Yield of unhulled rice was 20% more in the plot of treated water than that of chemical fertilizer.

젖소의 준임상형유방염 검진을 위한 Rolling Ball Viscometer법과 California Mastitis Test법의 비교시험 (Comparisons of the Rolling Ball Viscometer Test and California Mastitis Test for the Subclinical mastitis Screening Test of Dairy Cow)

  • 박동수;하영주;이주홍;조희택;강호조
    • 대한수의사회지
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    • 제23권8호
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    • pp.537-543
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    • 1987
  • In order to compare the diagnosis values the Rolling Ball Viscometer(RBV) and the California Mastitis Test(CMT) for detection of bovine subclinical mastitis, A total of 2204 quarter milk samples from 565 heads of Holstein in Gyeongnam area were examined d

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