Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Joh, Seong-Joon (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Choi, Kang-Seuk (Avian Diseases Division, Animal and Plant Quarantine Agency) ;
  • Kim, Aeran (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Seo, Min-Goo (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Young (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Yun, Seon-Jong (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency)
  • Received : 2016.04.18
  • Accepted : 2016.07.01
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Keywords

References

  1. Alexander DJ, Bracewell CD, Gough RE. Preliminary evaluation of the haemagglutination and haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathol 1976, 5, 125-134. https://doi.org/10.1080/03079457608418177
  2. Alexander DJ, Chettle NJ. Procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathol 1977, 6, 9-17. https://doi.org/10.1080/03079457708418208
  3. Alexander DJ, Gough RE. A long-term study of the pathogenesis of infection of fowls with three strains of avian infectious bronchitis virus. Res Vet Sci 1978, 24, 228-233.
  4. Blackman NJM, Koval JJ. Interval estimation for Cohen's kappa as a measure of agreement. Stat Med 2000, 19, 723-741. https://doi.org/10.1002/(SICI)1097-0258(20000315)19:5<723::AID-SIM379>3.0.CO;2-A
  5. Britton P, Armesto M, Cavanagh D, Keep S. Modification of the avian coronavirus infectious bronchitis virus for vaccine development. Bioeng Bugs 2012, 3, 114-119.
  6. Cavanagh D. Coronavirus IBV: structural characterization of the spike protein. J Gen Virol 1983, 64, 2577-2583. https://doi.org/10.1099/0022-1317-64-12-2577
  7. Cavanagh D, Davis PJ, Cook JKA, Li D, Kant A, Koch G. Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathol 1992, 21, 33-43. https://doi.org/10.1080/03079459208418816
  8. Cavanagh D, Gelb J. Infectious bronchitis. In: Saif YM, Barnes HJ (eds.). Diseasse of Poultry. 12th ed. Blackwell, Ames, 2008.
  9. Dawson PS, Gough RE. Antigenic variation in strains of avian infectious bronchitis virus. Arch Gesamte Virusforsch 1971, 34, 32-39. https://doi.org/10.1007/BF01250243
  10. de Wit JJ, Mekkes DR, Kouwenhoven B, Verheijden JH. Sensitivity and specificity of serological tests for infectious bronchitis virus antibodies in broilers. Avian Pathol 1997, 26, 105-118. https://doi.org/10.1080/03079459708419198
  11. King DJ, Hopkins SR. Evaluation of the hemagglutination-inhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination. Avian Dis 1983, 27, 100-112. https://doi.org/10.2307/1590376
  12. Kim BY, Lee DH, Jang JH, Lim TH, Choi SW, Youn HN, Park JK, Lee JB, Park SY, Choi IS, Song CS. Cross-protective immune responses elicited by a Korean variant of infectious bronchitis virus. Avian Dis 2013, 57, 667-670. https://doi.org/10.1637/10519-022213-ResNote.1
  13. Lashgari MS, Newman JA. Determination of the antigenic relationships within the Massachusetts (M41) type of infectious bronchitis virus using the hemagglutination-inhibition test. Avian Dis 1984, 28, 444-452. https://doi.org/10.2307/1590351
  14. Yan F, Zhao Y, Yue W, Yao J, Lihua L, Ji W, Li X, Liu F, Wu Q. Phylogenetic analysis of S1 gene of infectious bronchitis virus isolates from China. Avian Dis 2011, 55, 451-458. https://doi.org/10.1637/9446-070510-ResNote.1

Cited by

  1. Effect of high dietary levels of α-tocopherol acetate on immune response of light and heavy weight male broiler breeders vol.27, pp.5, 2018, https://doi.org/10.1007/s00580-018-2736-z