• 제목/요약/키워드: animal experiment

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Probing Equivocal Effects of Heat Processing of Legume Seeds on Performance of Ruminants - A Review -

  • Yu, P.;Tamminga, S.;Egan, A.R.;Christensen, D.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권6호
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    • pp.869-876
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    • 2004
  • Published studies show that effects of heat processing of legume seeds on animal performance are equivocal. In this article, we used a nutrition model - the DVE/OEB system to re-analyze nutrient supply (such as truly absorbed intestinal protein DVE value and protein degradation balance OEB value) to ruminants from published studies to probe reasons for such equivocal effects and provided some explanation why equivocal effects occurred. The analysis results showed that an unsuitable supply of nutrients in terms of DVE and OEB intakes (negative total OEB intake, oversupply of total DVE values) resulted in an inability to detect the effectiveness of heat processing in altering bypassing protein (BCP) and/or starch (BST) and their effects. The overall nutrient supply to animal in an experiment should be the context in which any animal performance study is developed. The information described in this article may give better understanding of animal performance in relation to nutritive changes occurring upon processing of legume seeds.

Effects of Betaine on Performence, Carcass Characteristics and Hepatic Betaine-homocysteine Methyltransferase Activity in Finishing Barrows

  • Feng, J.;Liu, X.;Wang, Y.Z.;Xu, Z.R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권3호
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    • pp.402-405
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    • 2006
  • This experiment was conducted to determine the effect of dietary betaine (0, 0.125%) on performance, carcass composition, pork quality and hepatic betaine-homocysteine methyltransferase (BHMT) activity of crossbred finishing barrows. Three replicates of ten pigs were used for each treatment. The results showed that average daily gain, feed intake and feed conversion were not affected by betaine. Compared with the control group, pigs treated with betaine had a 8.17% (p<0.05) decrease in carcass fat percentage, and a 8.84% (p<0.05) reduction in 10th-rib backfat thickness, but dressing percentage, percentage lean, longissimus muscle area, and average backfat thickness were not affected. There were also no significant differences in muscle color score, marbling score, pork pH value and water loss rate between the control and betaine-treated groups. Hepatic betaine-homocysteine methyltransferase (BHMT) activity was significantly increased by 13.97% (p<0.05) when pigs were offered 0.125% betaine.

임신돈 군사장치의 개발 (Development of an Electronic Sow Feeder for Gestation Sows)

  • 송준익;이준엽;천시내;김두환;박규현;전중환
    • 한국축산시설환경학회지
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    • 제19권2호
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    • pp.117-122
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    • 2013
  • The study was conducted to develop an ESF (electronic sow feeder) for improving animal welfare of group housed gestation sows. Feed intake of both individual and group housed gestation sows was restricted 2 kg a day. ESF system was very effective in reducing feed loss. The amount of feed loss in treatment group (group housed gestation sows with ESF) was only about 55 g, while the amount of feed loss in control group (individually housed gestation sows) was about 200 g. In spend time to feed intake, there was no difference in the result between the control (about 30 min) and the treatment (about 22 min) group. The ESF system ran well without any mechanical problems throughout the entire experiment. These results suggest that the EST is expected to improve the performance feeding of sows.

Gene Co-Expression Network Analysis of Reproductive Traits in Bovine Genome

  • Lim, Dajeong;Cho, Yong-Min;Lee, Seung-Hwan;Chai, Han-Ha;Kim, Tae-Hun
    • Reproductive and Developmental Biology
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    • 제37권4호
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    • pp.185-192
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    • 2013
  • Many countries have implemented genetic evaluation for fertility traits in recent years. In particular, reproductive trait is a complex trait and need to require a system-level approach for identifying candidate genes related to the trait. To find the candidate gene associated with reproductive trait, we applied a weighted gene co-expression network analysis from expression value of bovine genes. We identified three co-expressed modules associated with reproductive trait from bovine microarray data. Hub genes (ZP4, FHL2 and EGR4) were determined in each module; they were topologically centered with statistically significant value in the gene co-expression network. We were able to find the highly co-expressed gene pairs with a correlation coefficient. Finally, the crucial functions of co-expressed modules were reported from functional enrichment analysis. We suggest that the network-based approach in livestock may an important method for analyzing the complex effects of candidate genes associated with economic traits like reproduction.

Genetic Polymorphisms of the Bovine NOV Gene Are Significantly Associated with Carcass Traits in Korean Cattle

  • Kim, B.S.;Kim, S.C.;Park, C.M.;Lee, S.H.;Cho, S.H.;Kim, N.K.;Jang, G.W.;Yoon, D.H.;Yang, B.S.;Hong, S.K.;Seong, H.H.;Choi, B.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권6호
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    • pp.780-787
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    • 2013
  • The objective of this study was to investigate single nucleotide polymorphisms (SNPs) in the bovine nephroblastoma overexpressed (NOV) gene and to evaluate whether these polymorphisms affect carcass traits in the Korean cattle population. We resequenced to detect SNPs from 24 unrelated individuals and identified 19 SNPs within the full 8.4-kb gene, including the 1.5-kb promoter region. Of these 19 SNPs, four were selected for genotyping based on linkage disequilibrium (LD). We genotyped 429 steers to assess the associations of these four SNPs with carcass traits. Statistical analysis revealed that g.7801T>C and g.8379A>C polymorphisms in the NOV gene were associated with carcass weight (p = 0.012 and 0.008, respectively), and the g.2005A>G polymorphism was associated with the back fat thickness (BF) trait (p = 0.0001). One haplotype of the four SNPs (GGTA) was significantly associated with BF (p = 0.0005). Our findings suggest that polymorphisms in the NOV gene may be among the important genetic factors affecting carcass yield in beef cattle.

Effect of Production Phase on Growth, Enzyme Activities and Feed Selection of Broilers Raised on Vegetable Protein Diet

  • Hossain, M.A.;Islam, A.F.;Iji, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권11호
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    • pp.1593-1599
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    • 2014
  • This study consisted of two experiments, conducted to assess the impact of phase at which vegetable protein (VP) diets are introduced to broiler chicks, and preference of birds for diets based on soybean or canola meal (CM). Two hundred and ten day-old Cobb 500 chicks were randomly distributed into five dietary groups in the main experiment. One group was fed on animal protein (AP) diet all through to 21 days of age; two other groups were started on AP diet for 7 days and then switched to diets containing soybean meal (AP-SBM) or AP-CM, while two other diets (SBM-AP and CM-AP) were started on one of the VP diets for 7 days and then switched to AP diet. A sub-experiment on thirty birds raised on a commercial diet to 7 days was used in a feed selection test to quantify the preference of birds for the diets containing mainly CM or SBM. Chicks were reared under similar care and management conditions and the diets were iso-caloric and iso-nitrogenous. Results of the main experiment showed that chicks on CM-AP diet ate more (p<0.05) than those on the other diets up to day 7. Body weight gain was highest (p<0.001) on the AP-SBM diet while birds on the CM-AP diet weighed the least at 7 d. Feed intake, body weight gain, feed conversion ratio, mortality, bone growth, visceral organ development, and activities of digestive enzymes were similar between the groups from hatch to 21 days of age. Results of the second sub-experiment showed that chicks preferred the CM-based diets to the SBM-based diets at 8 to 14 d (p<0.001) and 15 to 21 d (p<0.01) when given a choice. Overall, the birds were not affected by the nature of the starter diet although they tended to prefer the canola to soybean diets.

Influence of Feeding Enzymatically Hydrolyzed Yeast Cell Wall on Growth Performance and Digestive Function of Feedlot Cattle during Periods of Elevated Ambient Temperature

  • Salinas-Chavira, J.;Arzola, C.;Gonzalez-Vizcarra, V.;Manriquez-Nunez, O.M.;Montano-Gomez, M.F.;Navarrete-Reyes, J.D.;Raymundo, C.;Zinn, R.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권9호
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    • pp.1288-1295
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    • 2015
  • In experiment 1, eighty crossbred steers ($239{\pm}15kg$) were used in a 229-d experiment to evaluate the effects of increasing levels of enzymatically hydrolyzed yeast (EHY) cell wall in diets on growth performance feedlot cattle during periods of elevated ambient temperature. Treatments consisted of steam-flaked corn-based diets supplemented to provide 0, 1, 2, or 3 g EHY/hd/d. There were no effects on growth performance during the initial 139-d period. However, from d 139 to harvest, when 24-h temperature humidity index averaged 80, EHY increased dry matter intake (DMI) (linear effect, p<0.01) and average daily gain (ADG) (linear effect, p = 0.01). There were no treatment effects (p>0.10) on carcass characteristics. In experiment 2, four Holstein steers ($292{\pm}5kg$) with cannulas in the rumen and proximal duodenum were used in a $4{\times}4$ Latin Square design experiment to evaluate treatments effects on characteristics of ruminal and total tract digestion in steers. There were no treatment effects (p>0.10) on ruminal pH, total volatile fatty acid, molar proportions of acetate, butyrate, or estimated methane production. Supplemental EHY decreased ruminal molar proportion of acetate (p = 0.08), increased molar proportion of propionate (p = 0.09), and decreased acetate:propionate molar ratio (p = 0.07) and estimated ruminal methane production (p = 0.09). It is concluded that supplemental EHY may enhance DMI and ADG of feedlot steers during periods of high ambient temperature. Supplemental EHY may also enhance ruminal fiber digestion and decrease ruminal acetate:propionate molar ratios in feedlot steers fed steam-flaked corn-based finishing diets.

Effects of Early Feed Restriction on Breast and Leg Meat Composition and Plasma Lipid Concentration in Unsexed Broiler Chickens Reared in Cages

  • Santoso, U.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권10호
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    • pp.1475-1481
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    • 2002
  • The study was conducted to evaluate the effect of early feed restriction on growth, fat accumulation and meat composition in unsexed broiler chickens. In experiment 1, three hundred and fifty one-day-old broiler chicks were divided into 7 groups. Each treatment group was represented by five replicates of ten broilers each. One group was fed ad libitum as the control group and the other six groups were fed 25% ad libitum (25% multiplied by amount of feed intake of ad libitum chicks at the previous day) for 4 or 6 days, 50% ad libitum for 4 or 6 days, and 75% ad libitum for 4 or 6 days. In experiment 2, five hundred broiler chicks were divided into 10 groups. Each treatment group was represented by five replicates of ten broilers each. One group was fed ad libitum as the control group. Three initial age at which broilers were restricted (2, 4 or 6 days of age) and three type of feed restriction (physical restriction, meal feeding and diet dilution) ($3{\times}3$) were examined. They were restricted feeding for 6 days. Experimental results showed that broilers fed 25% ad libitum for 4-6 days tended to reduce leg meat fat with lower abdominal fat (p<0.05) (experiment 1). Breast meat fat was significantly higher in restricted broilers (p<0.01). Plasma triglyceride was higher in broilers fed 75% ad libitum for 4-6 days. In experiment 2, abdominal fat was lower in restricted broilers (p<0.05). Breast meat fat was significantly higher (p<0.01), whereas leg meat fat was significantly lower (p<0.05) in restricted broilers. Plasma triglyceride was significantly higher in physical feed restriction for 4 days, meal feeding for 4 days and diet dilution for 6 days (p<0.05). In conclusion, to reduce fat accumulation in abdomen and leg meat, broilers should be fed 25% ad libitum for 6 days started at 4 days, or subjected to meal feeding (6 hours per day for 6 days) started at 6 days.

Effects of Early Feed Restriction on the Occurrence of Compensatory Growth, Feed Conversion Efficiency, Leg Abnormality and Mortality in Unsexed Broiler Chickens Reared in Cages

  • Santoso, U.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권9호
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    • pp.1319-1325
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    • 2002
  • Two experiments were conducted to evaluate the effect of early feed restriction on growth, feed conversion ratio (FCR) and mortality in unsexed broiler chickens. In Experiment 1, 350 one-day-old broiler chickens were divided into 7 groups. Each treatment group was represented by five replicates of ten broilers each. One group was fed ad libitum as the control group and the other six groups were fed 25% ad libitum (25% multiplied by amount of feed intake of ad libitum chickens at the previous day) for 4 or 6 days, 50% ad libitum for 4 or 6 days, and 75% ad libitum for 4 or 6 days. In experiment 2, 500 broiler chickens were divided into 10 groups. Each treatment group was represented by five replicates of ten broilers each. One group was fed ad libitum as the control group. Three ages of feed restriction initial timing (2, 4 or 6 days of age) and three types of feed restriction (physical restriction, meal feeding and diet dilution) were used ($3{\times}3$) in both experiments. They were feed-restricted for 6 days. Results showed that restricted broilers exhibited compensatory growth in both experiments except for diet dilution groups. In both experiments, FCR of restricted broilers was higher, whereas feed intake of them was lower during restriction period. FCR of restricted broilers was lower upon refeeding. Mortality was inconsistently affected by early feed restriction. Leg abnormality was lower in restricted broilers in both experiments. Level of feed restriction significantly influenced body weight, FCR and feed intake of restricted broilers (p<0.05), but duration of feed restriction had no effect. Type of restriction significantly affected body weight, feed intake and FCR (p<0.05). It appeared that to achieve the best result (complete compensatory growth and better FCR), broilers should be restricted at 25% ad libitum for 6 days (Experiment 1). Meal feeding started at 2 days of age would show the best performance (Experiment 2).