• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.897-902
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• 2004

To improve the methods used for the identification of Hanwoo, we performed a PCR using 3 -tailed primer associated with single nucleotide polymorphism(SNP) in Melanocortin 1 receptor(MCIR) gene. MCIR plays an important role in melanin synthesis and the SNP within MCIR was used as a target for PCRRFLP studies previously. A forward 3 -tailed primer, which matches with the template DNA of Hanwoo but not with others(blackhaired; Holstein and Black angus) at the site of 594th base sequence, and one reverse primer were designed for this study. When use this primer set, a size of 343bp was amplified by PCR only in Hanwoo, not in Holstein and Black angus. This result suggests that the PCR using our 3 -tailed primer would be very accurate, easy, reproducible and economic method to discriminate between Hanwoo meat and other black-haired ones.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.172-176
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• 2004

본 연구는 축우의 모색발현을 조절하는 MCIR, MGF 및 TYRP1 3종류의 모색 유전자를 이용하여 한우육 판별기술을 개발하고자 PCR-RFLP 기법으로 이들 모색유전자 좌위의 대립유전자를 검출하고 각 품종 간 RFLP 유전자형 출현빈도를 비교 분석하였다. MCIR 유전자의 RFLP 유전자형 출현빈도에서 한우는 e/e과 E+/e형이 출현되었고 이외의 다른 유전자형의 출현은 전혀 인정되지 않았다. 그러나, Holstein종 젖소는 $E^D/E^D$와 $E^D/e$ 2종류의 유전자형 그리고 Angus종에서는 $E^D/E^D$, $E^D/E^++$ 및 $E^D/e$ 3종류의 유전자형이 각각 출현하여 한우와 이들 두 품종간의 MCIR유전자형 출현빈도에 뚜렷한 차이가 인정되었다. MGF 유전자의 RFLP 유전자형 출현빈도에서 한우는 R/r과 r/r형이 각각 25%와 75%로 rr형의 출현율이 비교적 높았으며 Holstein종과 Angus 종은 R/r형이 100% 출현했으며, Charolais 종은 rr형이 100% 출현하였고 이외의 다른 유전자형은 인정되지 않았으며 Hereford종은 RR형이 80% 그리고 R/r형이 20%의 출현율을 보여 RR형의 출현율이 매우 높아 한우와 Holstein 및 육우 품종간의 MGF 유전자형 출현빈도에 명백한 차이가 인정되었다. 따라서, 소 모색관련 MCIR과 MGF 유전자의 품종 특이적 PCR-RFLP 유전자형은 한우육과 국내산 Hostein 젖소육 및 도입육우 품종을 식별하는데 매우 유용한 DNA marker로 이용될 수 있음이 확인되었다.

• Food Science of Animal Resources
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• v.31 no.5
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• pp.772-781
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• 2011

This study was conducted to investigate the proximate composition, meat color, Warner-Bratzler shear force (WBS), total collagen content (%), cooking loss (CL), fatty acid composition, amino acid composition, and mineral content of loin, strip loin, top round, and chuck tender Hanwoo beef and imported Australian beef. Protein content was significantly lower for strip loin and loin of Hanwoo QG $1^+$ and 1 beef when compared to that of Australian black Angus or cross beef (p < 0.05), whereas it was not significantly different for top round and chuck tender. Intramuscular fat content was higher for QG $1^+$ Hanwoo beef strip loin (15.48%) than that in Australian cross beef (8.83%) and it was also higher in loin for QG $1^+$ (17%) and 1 (15.52%) Hanwoo beef than that in Australian Angus beef (10.59%) and cross beef (9.21%) (p < 0.05). The CIE $L^*$ value was significantly higher for Australian cross beef strip loin than that of Hanwoo beef but the CIE $a^*$ value was not significantly different between the same cuts from different origins. However, CIE $b^*$ values were significantly higher for the Australian cross beef than those for four cuts of Hanwoo beef and Australian Angus beef (p < 0.05). No significant difference in WBS of loin, top round, or chuck tender was observed among the different beef sample origins except that strip loin had significant higher WBS for Australian cross beef (3.02 kg) but lower for Australian Angus beef (2.13 kg). Australian cross beef contained significantly higher palmitic acid, stearic acid, linolenic acid, but lower palmitoleic acid, oleic acid, eicosenoic acid content in strip loin, loin, and top round than those of QG $1^+$ and 1 Hanwoo beef (p < 0.05). QG $1^+$ Hanwoo beef had significant lower saturated fatty acid and higher monounsaturated fatty acid content than those in Australian cross beef (p < 0.05). Hanwoo $1^+$ beef had more glycine in top round and chuck tender, whereas Hanwoo QG 1 beef and Australian Angus beef had higher cysteine, methione, and glycine levels in strip loin and loin and valine and leucine in top round and chuck tender than the same cuts of Hanwoo $1^+$ beef (p < 0.05). No significant differences were observed for Ca, Fe, or Zn content among the different beef samples.

• Journal of Ginseng Research
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• v.27 no.3
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• pp.141-145
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• 2003

The multi-year production cycle for ginseng can be rapidly depreciated by inferior post-harvest activities. This research examines the character of high temperature drying regimes for North American ginseng root to assist management decision making. The objective is a very rapid drying regime, that will not result in physical or chemical damage to the root and that would not alter the actual dry root weight. Research is presented using drying temperatures of 55, 70 and 105 C. Temperatures above these rapidly cause substantive physical damage to the root samples and seriously compromise the dry root values determined. Temperatures below these behaved quite similar to actual dryer regimes (approximately 38 C). Laboratory results indicate that there are differences between the three temperature regimes tested. Careful usage of the 70 C regime, over a period of two to three days in a convection drying oven, has distinct merit.

• Journal of Embryo Transfer
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• v.9 no.3
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• pp.221-227
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• 1994

This study was conducted to examine the condition of in vitro culture system and the viability after embryo transfer of in vitro matured-in vitro fertilized (IVM-IVF) bovine embryos. The in vitro development to the blastocyst stage was enhanced by supplying bovine serum albumin(BSA) to co-culture medium with bovine oviduct epithelial tissue(BOET) compared with that in medium supplemented with fetal bovine serum(FBS) (41.2% vs. 26. 3%, P<0.05). After transfer of IVM-IVF blastocysts into the uterine horn of recipient females (Aberdeen Angus), one was pregnant to term and produced a head of male Korean native calf. These results confirm that the in vitro development of IVM-IVF bovine embryos is affected with different protein source in co-culture with BOET, and IVM-IVF embryos can develop to term after in vitro culture and embryo transfer.

• Journal of the korean veterinary medical association
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• v.16 no.1112
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• pp.363-367
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• 1980

An annual incidence of disease and mortality rate was surveyed to the 573 imported beef cattle in sunchun district, Jeonnam during 1979 inorder to evaluate to advanced animal health control and laboratory reutine examinations for piroplasmosis and interna

• Fisheries and Aquatic Sciences
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• v.10 no.4
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• pp.215-219
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• 2007

Long-tenn variation in krill (Euphausia superba) and salp (mainly Salpa thompsoni) stocks was compared to environmental changes in the Atlantic and Indian sectors of the Antarctic. Environmental conditions examined were air temperature, water temperature, salinity, and sea-ice extent from 1926 to 1938 and from 1982 to 2000. The long-term pattern of krill was opposite to that of salp: krill stock decreased while salp stock increased concurrently. Krill stock was about three-fold higher from 1926 to 1938 than from 1982 to 2000, but salp was about four -fold lower in 1926-1938 than in 1982-2000. A wanning trend was observed in the environmental data, and the long-term variation in krill and salp stocks was affected by this trend.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2006.05a
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• pp.192-195
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• 2006

Current study was conducted to investigate the effect of different hanging techniques (achilles tendon, aitch bone and pelvic ligament) on variations in sarcomere length and palatability within and between muscles using thirty-four Angus steer sides. Results showed that tenderstretch(by either suspension method) resulted in longer sarcomeres for most positions within the major leg muscle than did conventional hanging method, but in some minor muscles(eg., mm. gluteus profundus, gastronemius, and gracilis) tenderstetch allowed the muscles fibres to shorten. Some tenderstretched muscles(e.g., m. gluteus profundus, 1.5 ${\mu}m$) appeared not to toughen even at very low sarcomere lengths, while others toughened at higher sarcomere lengths. The current data demonstrated that the effect of tenderstretch on the length of sarcomeres and its influence on palatability varied between muscles, Overall the difference between the two tenderstretch methods was for the aitch method to produce meat that was 3.2 units more palatable than the ligament hanging method.

• Child Studies in Asia-Pacific Contexts
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• v.3 no.1
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• pp.1-12
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• 2013

Research with parents of children with Asperger Syndrome was conducted to assess whether the level of positive parental attachment correlated positively with positive parenting behaviors and negatively with negative parenting behaviors. Participants were recruited from internet. The Parental Bonding Inventory measured parents' perception of their bonding or attachment with three aspects of their own parents: warmth, control, and care. In the Parenting Behavior Inventory, parents reported recent interaction/reaction behaviors with their child, and results focused on two aspects of parenting, supportive/engaged and hostile/coercive behaviors: each identified as problematic to parenting and attributable to a variety of specific parenting behaviors. Analysis of demographic variables for correlations with positive parenting behaviors and negative parenting behaviors were carried out by Pearson correlations. Two separate standard multiple regressions, one for positive parenting behaviors and one for negative parenting behaviors, were conducted. Findings support the hypothesis that positive early attachment experience of parents has a significant impact upon their own positive parenting skills with their child with Asperger Syndrome. However, multiple regression of negative parenting behavior found no significant negative contribution by parental attachment. Demographic variables proved to be important.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.653-660
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• 2002

We considered KIT gene as a candidate gene for the white-spotting pattern in cattle. This study was carried out to detect genetic variation of c-KIT receptor gene and to investigate association between the mutation and the white-spotting pattern in cattle. PCR-RFLP analysis within intron 6 of c-KIT receptor gene were performed with 8 cattle breeds including Hanwoo, Angus, Brown Swiss, Charolais, Hereford, Holstein, Limousin and Simmental. When PCR product of approximately 2,440 bp including intron 6 of c-KIT receptor gene was sequenced, four nucleotide substitutions were found within intron 6 of the bovine c-KIT receptor gene. In PCR-RFLP analysis, three alleles (A, B and C), two alleles (A and B) and two alleles (A and B) at each locus were identified by MspⅠ, BsrBⅠ and NdeⅠ, respectively. Although frequencies of allele at each locus were different among cattle breeds, we could not get any evidence related with white or white spotting phenotypes in these mutations on intron 6 of c-KIT receptor gene. However, we can not entirely exclude the possibility that c-KIT receptor gene is responsible for white spotting phenotype in cattle. Thus, further studies need to detect other mutations in c-KIT receptor gene and to test association of those mutations and coat color phenotypes in cattle.