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http://dx.doi.org/10.5187/JAST.2004.46.6.897

Identification of Hanwoo Using 3'-tailed Primer Associated with Single Nucleotide Polymorphism(SNP) in Melanocortin 1 Receptor(MC1R) gene  

Kim, T.J. (College of Veterinary Medicine, Chonnarn National University)
Park , S.D. (College of Veterinary Medicine, Chonnarn National University)
Lee , J. I. (College of Veterinary Medicine, Chonnarn National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.6, 2004 , pp. 897-902 More about this Journal
Abstract
To improve the methods used for the identification of Hanwoo, we performed a PCR using 3 -tailed primer associated with single nucleotide polymorphism(SNP) in Melanocortin 1 receptor(MCIR) gene. MCIR plays an important role in melanin synthesis and the SNP within MCIR was used as a target for PCRRFLP studies previously. A forward 3 -tailed primer, which matches with the template DNA of Hanwoo but not with others(blackhaired; Holstein and Black angus) at the site of 594th base sequence, and one reverse primer were designed for this study. When use this primer set, a size of 343bp was amplified by PCR only in Hanwoo, not in Holstein and Black angus. This result suggests that the PCR using our 3 -tailed primer would be very accurate, easy, reproducible and economic method to discriminate between Hanwoo meat and other black-haired ones.
Keywords
MC1R gene; Coat color; Tailed-primer; Hanwoo;
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