• 한국수의병리학회지
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• 제1권1호
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• pp.13-25
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• 1997

The anticarcinogenic effects of dimethyl disulfide(DMDS, methyl disulfide)and diallyl disulfide(DADA, allyl disulfide) were studied in a 28 weeks rat multi-organ carcinogenesis model. neoplastic and preneoplastic lesions were observed in the liver kidney thyroid gland esophagus duodenum colon, rectum and adrenal gland. Tesults showed that neoplastic lesions in the kidney liver and thyroid gland were inhibited by DADS but those in the liver and colon were enhanced by DMDS when compared to positive control group. incidence of neoplastic lesions in the other organs were not changed by DMDS or DADS exposure. While GST-p positive foci in the liver were increased by DMDS, DADS had no effect. There was no significant histopathological lesion in DMDS or DADS treated group without pretreatment with carcinogens.

• Journal of Korean Society for Atmospheric Environment
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• 제18권E3호
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• pp.165-174
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• 2002

A dual -column biofilter system with two different composts was used to investigate the macro-kinetics of dim-ethyl disulfide (DMDS) degradation. The biofilter columns were filled with compost mixtures up to one meter, The gas How rate and DMDS concentration to the biofilters were varied to study their effect on the removal characteris-tics of DMDS. It was found that the biodegradation of DMDS was governed by zero-order reaction -limited macro-kinetics for inlet DMDS concentrations between 10 and 55 ppmv. The overall average zero-order kinetic coeffi-cient for DMDS removal by compost was 0.50 ($\pm$0.1) ppm/sec for both compost mixtures studied. Variations in individual kinetic coefficients were observed due to varying environmental conditions, such as pH and temperature. The kinetic coefficients determined are specific to the system discussed in this work. During high acidity conditions in the filter beds, methyl mercaptan (MM) was observed in the gas samples collected. Appearance of MM was pro-bably due to decreased microbial activity in the lower portions of the biofilter. Considering the neutral pH range required and the presence of methyl mercaptan, it is likely that the microorganisms present in the biofilters used in this research are similar to the T. thioparus (strain E6) species.

• 한국환경과학회지
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• 제11권8호
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• pp.783-791
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• 2002

A laboratory-scale dual-column biofilter system was used to study the biofiltration of dimethyl disulfide(DMDS). The biofiltration of DMDS was found to depend on the pollutant loadings rather than the inlet concentrations. It was estimated that the pollutant was only inhibitory to the operation of the biofilters at DMDS concentrations greater than 5500 ppmv A residence time of 30 seconds(120 m$^3$/m$^2$/h volumetric loading) was determined as appropriate for efficient operation(>90%). The maximum elimination capacity for both compost mixtures under the current experimental conditions was found to range from 7.5 to 10 g-DMDS/m$^3$/h. A lower DMDS maximum elimination capacity was exhibited under acidified conditions.

• Toxicological Research
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• 제9권2호
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• pp.167-175
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• 1993

Protective effects of dithiol malonate derivatives (DMDs), YH-100, YH-150 and YH-439 on carbon tetrachloride-induced hepatotoxicity were investigated in primary rat hepatocytes culture. Treatment of DMDs to hepatocytes culture did not affect total cytochrome P-450 content and ECOD and AHH activities. Protein and RNA synthesis was also similar to control. Meanwhile, DMDs significantly decreased LDH release and in vitro lipid peroxidation induced by $CCI_4$. Accumulation of cellular triglyceride and decreased secretion of VLDL from liver cells by $CCI_4$ treatment were also significantly protected.

• Journal of Korean Society for Atmospheric Environment
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• 제18권E3호
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• pp.153-163
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• 2002

A laboratory- scale dual-column biofilter system was used to study the biofiltration of dimethyl disulfide (DMDS). The gas flow rate and DMDS concentration to the biofilter were varied to study their effect on the remov-al of dimethyl disulfide. Operating parameters such as pH, temperature, and water content were monitored during the biofilter operation and necessary precautions were taken to keep these parameters within the acceptable limits. It was observed that the removal efficiency of DMDS was optimal at neutral pH values. After five month op-eration, the neutralization of the filter beds with sodium carbonate became necessary for the optimum operation of the biofilters. The microbial population already present in the compost mixtures was found to be adequate in treat-ing DMDS. The compost mixtures were found to be similar in terms of biofiltration efficiency of DMDS. However, pressure drops observed in the first column compost mixture (compost/ peat mulch) was extremely high, making this compost economically not feasible. The second mixture (compost/bark) provided pressure drops within accept-able limits. A minimum residence time of 30 seconds at the optimal operating conditions appeared to be adequate for achieving high removal efficiencies (>90%).

• 한국환경과학회지
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• 제18권11호
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• pp.1215-1224
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• 2009

This study evaluated the applicability of visible-light-driven N- and S-doped titanium dioxide($TiO_2$) for the control of low-level dimethyl sulfide(DMS) and dimethyl disulfide(DMDS). In addition, a photocatalytic unit(PU)-adsorption hybrid was evaluated in order to examine the removal of DMS and DMDS which exited the PU and a gaseous photocatalytic byproduct($SO_2$) which was generated during the photocatalytic processes. Fourier-Tranform-Infrared(FTIR) spectrum exhibited different surface characteristics among the three-types of catalysts. For the N- and S-doped $TiO_2$ powders, a shift of the absorbance spectrum towards the visible-light region was observed. The absorption edge for both the N- and S-doped $TiO_2$ was shifted to $\lambda$ 720 nm. The N-doped $TiO_2$ was superior to the S-doped $TiO_2$ in regards to DMS degradation. Under low input concentration(IC) conditions(0.039 and 0.027 ppm for DMS and DMDS, respectively), the N-doped $TiO_2$ revealed a high DMS removal efficiency(above 95%), but a gradual decreasing removal efficiency under high IC conditions(7.8 and 5.4 ppm for DMS and DMDS, respectively). Although the hybrid system exhibited a superior characteristic to PU alone regarding the removal efficiencies of both DMS and DMDS, this capability decreased during the course of a photocatalytic process under the high IC conditions. The present study identified the generation of sulfate ion on the catalyst surface and sulfur dioxide(maximum concentrations of 0.0019 and 0.0074 ppm for the photocatalytic processes of DMS and DMDS, respectively) in effluent gas of PU. However, this generation of $TiO_2$ would be an insignificant addition to indoor air quality levels.

• Toxicological Research
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• 제3권2호
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• pp.97-110
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• 1987

The effects of 5 novel hepatotrophic agents, dithiol malonate derivatives (DMDs; DMD1-DMD5), on the liver microsomal lipid peroxidation induced by carbon tetrachloride $(CCl_4)$ and the correlations with the changes of microsomal electron transport system were investigated. All DMDs were found to inhibit the lipid peroxidation induced by $CCl_4$ in mice and rats as well in vitro liver microsomal system. Therefore, each DMD seemed to have direct mode of action on liver microsomes to inhibit the lipid peroxidation. As an ex vivo study, the induced lipid peroxidation by $CCl_4$ and the changes in electron transport system were determined with liver microsomes obtained from rats chronically treated with DMDs for 7 days. The induced lipid peroxide contents in liver microsomal system were lower in DMD1, DMD2 and DMD3 treated group, but higher in DMD4 and DMD5 group when compared to the control group. Cyt. p.450 contents in the microsomes were decreased by the treatment with DMD1, DMD2 and DMD3, but increased significantly by DMD4 with great extent and by DMD5 with less extent. The cyt. p-450 isozymes induced by treatment of DMD4 and DMD5 were identified as 3-methylcholanthrene (MC) type. The NADPH cyt. -C reductase activities of the microsomes treated with DMD1, DMD2, DMD4 and DMD5 were increased in the range of around 20% to 50%, but decreased with DMD3, All DMDs increased dyt. $-b_5$ content and did not alter NAdH-cyt, $-b_5$ reductase activities in the microsomes. In summary, the 5 novel hepatotrophic agents (DMDs) markedly protected against lipid peroxidation induced by $CCl_4$ in vivo and in vitro possibly through the mechanism of direct action on the liver microsomes. The degree of inhibition produced by DMDs on lipid peroxidation induced by $CCl_4$ seemed to coincide rather with cyt. p-450 contents than with other components of liver microsomal electron transport system including NADPH-cyt, -C reductase.

• 분석과학
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• 제24권3호
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• pp.212-218
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• 2011

본 연구에서는 5종류의 황화합물질($H_2S$, $CH_3SH$, DMS, $CS_2$, DMDS)을 10 ppb (nmol/mol) 수준으로 포함하는 기체상 표준시료를 Teldlar bag에 담은 후 시차를 두고 30일 동안의 기간에 걸쳐 농도변화를 분석하였다. 황성분의 시간적 안정도를 파악하기 위해, 백에 보존한 시료의 보존방식과 분석시점에 대한 결정을 두 가지 상이한 접근방식으로 비교하였다. 이를 통해 시료의 장기적 보관방식과 분석시스템의 일관성에 대한 특성을 동시에 평가하고자 하였다. 이를 위하여, 첫 번째로 전체 보관기간 동안 분석할 시료를 일괄적으로 준비하여 이들을 시간대별로 하나씩 분석하는 Forward(F)보전방식으로 진행하였다. 두 번째로는 시료를 시간의 경과에 따라 순차적으로 준비하고, 종료시점에 모두 일괄적으로 분석하는 Reverse(R)방식으로 접근하였다. 이를 유의수준 90% 기준으로 t-테스트를 적용하여, 두 가지 분석방식의 자료들간에 차이점을 짝비교 방식으로 검정하였다. 이때 $H_2S$, $CH_3SH$, DMDS성분의 결과는 유의한 차이가 존재하는 것으로 나타났다. 본 실험의 결과, 분자량이 가벼운 황성분들($H_2S$와 $CH_3SH$)과 무거운 황성분들(DMS, $CS_2$, DMDS)간의 경시적 안정도에는 현저한 차이가 존재하는 것을 확인였다. 그러나 두 방식 모두 농도가 감소하는 상대적 경향은 성분 별로 일치하였다.

• 환경생물
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• 제41권4호
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• pp.455-462
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• 2023

고구마뿌리혹선충(Meloidogyne incognita)은 국내 오이 재배지에서 문제되고 있는 식물기생선충으로 연간 500억원의 경제적 피해를 일으키고 있다. 국내에서는 시설재배지 문제 고구마뿌리혹선충의 방제를 위해 화학적 살선충제를 주로 이용하고 있다. 본 연구는 겨울철 휴경기 화학적 살선충제를 이용하여 오이 시설재배지에 문제되고 있는 고구마뿌리혹선충의 사전 방제 효과를 확인하기 위해 수행하였다. 2022년 10월부터 12월, 전북 장수군 천천면 소재 오이 비닐하우스에서 훈증성 살선충제인 디메틸디설파이드(dimethyl disulfide, DMDS)와 비훈증성 살선충제인 플루오피람(fluopyram, FL)을 처리하여 고구마뿌리혹선충 방제 효과를 검정하였다. 살선충제의 방제 효과는 약제처리 43일 경과 후 평가하였으며, 오이의 뿌리혹지수는 정식 4개월 후 평가하였다. 그 결과, DMDS는 고구마뿌리혹선충에 대한 91%의 방제 효과를 보였으나, 플루오피람은 방제 효과가 없었다. 뿌리혹지수는 무처리(4.3±0.58) 대비 DMDS 처리구(1.0±0.00)에서 현저히 낮았으며 통계적으로 유의미한 차이를 보였다(p=0.020). 연구 결과에 따라 DMDS는 겨울철 휴경하는 오이 시설재배 작형의 고구마뿌리혹선충 방제에 효과적인 살선충제로 판단된다.

• 한국환경농학회지
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• 제33권4호
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• pp.381-387
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• 2014

BACKGROUND: Garlic (Allium sativum) extract has been allowed as commercial biopesticide material for pesticidal activity in the Environmentally-friendly Agriculture Promotion Act. Nine commercial biopesticides containing A. sativum extract have been marketed in Korea. However, the analytical method of the active substances in these materials has not been studied. METHODS AND RESULTS: Cartridge clean-up method for the determination of dimethyl disulfide(DMDS), diallyl disulfide(DADS), and diallyl trisulfide(DATS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography(GC). The clean-up method was optimized using hydrophilic lipophilic balance (HLB) solid phase extraction(SPE) cartridges for the bioactive sulfides in biopesticides containing A. sativum extract, and the eluate was analyzed to quantify the DMDS, DADS, and DATS using the GC. The developed method was validated, and the LOQ and recovery rates of DMDS, DADS, and DATS were 0.226, 0.063, and $0.051mg\;L^{-1}$ and 80.6, 84.8, and 73.1%, respectively. From the nine commercial biopesticide samples, contents of DMDS, DADS, and DATS were analyzed using the developed method and results showed $2.3mg\;L^{-1}$, respectively. CONCLUSION: The developed method could be used in determining the quality of biopesticides for the manufacture of commercial biopesticides containing A. sativum extract.