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http://dx.doi.org/10.5338/KJEA.2014.33.4.381

Determination of Dimethyl Disulfide, Diallyl Disulfide, and Diallyl Trisulfide in Biopesticides Containing Allium Sativum Extract by Gas Chromatography  

Lim, Sung-Jin (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Ji-Hye (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Jin-Hyo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Cho, Geun-Hyoung (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Cho, Nam-Jun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Park, Byung-Jun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Environmental Agriculture / v.33, no.4, 2014 , pp. 381-387 More about this Journal
Abstract
BACKGROUND: Garlic (Allium sativum) extract has been allowed as commercial biopesticide material for pesticidal activity in the Environmentally-friendly Agriculture Promotion Act. Nine commercial biopesticides containing A. sativum extract have been marketed in Korea. However, the analytical method of the active substances in these materials has not been studied. METHODS AND RESULTS: Cartridge clean-up method for the determination of dimethyl disulfide(DMDS), diallyl disulfide(DADS), and diallyl trisulfide(DATS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography(GC). The clean-up method was optimized using hydrophilic lipophilic balance (HLB) solid phase extraction(SPE) cartridges for the bioactive sulfides in biopesticides containing A. sativum extract, and the eluate was analyzed to quantify the DMDS, DADS, and DATS using the GC. The developed method was validated, and the LOQ and recovery rates of DMDS, DADS, and DATS were 0.226, 0.063, and $0.051mg\;L^{-1}$ and 80.6, 84.8, and 73.1%, respectively. From the nine commercial biopesticide samples, contents of DMDS, DADS, and DATS were analyzed using the developed method and results showed , respectively. CONCLUSION: The developed method could be used in determining the quality of biopesticides for the manufacture of commercial biopesticides containing A. sativum extract.
Keywords
Allium sativum; Biopesticide; Diallyl disulfide; Diallyl trisulfide; Dimethyl disulfide;
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