• Food Science of Animal Resources
• /
• v.37 no.1
• /
• pp.134-138
• /
• 2017

Salmonella enterica infects a broad range of host animals, and zoonostic infection threatens both public health and the livestock and meat processing industries. Many antimicrobials have been developed to target Salmonella envelope that performs essential bacterial functions; however, there are very few analytical methods that can be used to validate the efficacy of these antimicrobials. In this study, to develop a potential biosensor for Salmonella envelope stress, we examined the transcription of the S. enterica serovar typhimurium spy gene, the ortholog of which in Escherichia coli encodes Spy (${\underline{s}}pheroplast$ ${\underline{p}}rotein$ ${\underline{y}}$). Spy is a chaperone protein expressed and localized in the periplasm of E. coli during spheroplast formation, or by exposure to protein denaturing conditions. spy expression in S. typhimurium was examined by constructing a spy-gfp transcriptional fusion. S. typhimurium spy transcription was strongly induced during spheroplast formation, and also when exposed to membrane-disrupting agents, including ethanol and the antimicrobial peptide polymyxin B. Moreover, spy induction required the activity of regulator proteins BaeR and CpxR, which are part of the major envelope stress response systems BaeS/BaeR and CpxA/CpxR, respectively. Results suggest that monitoring spy transcription may be useful to determine whether a molecule particularly cause envelope stress in Salmonella.

• Journal of Digital Convergence
• /
• v.16 no.1
• /
• pp.337-342
• /
• 2018

This study was started to study the artistic aesthetic value of the antique artwork which is gradually declining in the field of metal crafts. The aesthetic interpretation of the lines and faces that have been identified through previous researches has been defined as a vitality and repetitive beauty. The researchers here have tried to express the formative works which can be repeated and change. As a result, it was found that the object of inquiry which can express the aesthetic beauty felt in the simple change of the line and the face is possible without the advanced technique of the difficult technique or the molding method through the natural and gradual change of the line and the face rather than the regular repetition. Through this study, it was found that the use of future lines and surfaces could be utilized as a variety of aesthetic expression methods as molding elements.

• Molecules and Cells
• /
• v.43 no.7
• /
• pp.645-661
• /
• 2020

Leaf senescence is a developmental process by which a plant actively remobilizes nutrients from aged and photosynthetically inefficient leaves to young growing ones by disassembling organelles and degrading macromolecules. Senescence is accelerated by age and environmental stresses such as prolonged darkness. Phytochrome B (phyB) inhibits leaf senescence by inhibiting phytochrome-interacting factor 4 (PIF4) and PIF5 in prolonged darkness. However, it remains unknown whether phyB mediates the temperature signal that regulates leaf senescence. We found the light-activated form of phyB (Pfr) remains active at least four days after a transfer to darkness at 20℃ but is inactivated more rapidly at 28℃. This faster inactivation of Pfr further increases PIF4 protein levels at the higher ambient temperature. In addition, PIF4 mRNA levels rise faster after the transfer to darkness at high ambient temperature via a mechanism that depends on ELF3 but not phyB. Increased PIF4 protein then binds to the ORE1 promoter and activates its expression together with ABA and ethylene signaling, accelerating leaf senescence at high ambient temperature. Our results support a role for the phy-PIF signaling module in integrating not only light signaling but also temperature signaling in the regulation of leaf senescence.

• Journal of Powder Materials
• /
• v.17 no.3
• /
• pp.190-196
• /
• 2010

In this paper, rapid solidified Mg-4.3Zn-0.7Y (at.%) alloy powders were prepared using an inert gas atomizer, followed by a severe plastic deformation technique of high pressure torsion (HPT) for consolidation of the powders. The gas atomized powders were almost spherical in shape, and grain size was as fine as less than $5\;{\mu}m$ due to rapid solidification. Plastic deformation responses during HPT were simulated using the finite element method, which shows in good agreement with the analytical solutions of a strain expression in torsion. Varying the HPT processing temperature from ambient to 473 K, the behavior of powder consolidation, matrix microstructural evolution and mechanical properties of the compacts was investigated. The gas atomized powders were deformed plastically as well as fully densified, resulting in effective grain size refinements and enhanced microhardness values.

• ETRI Journal
• /
• v.37 no.3
• /
• pp.450-459
• /
• 2015

Small cell networks, as an important evolution path for next-generation cellular networks, have drawn much attention. Different from the traditional base stations (BSs) always-on model, we proposed a BSs on-off model, where a new, simple expression for the probabilities of active BSs in a heterogeneous network is derived. This model is more suitable for application in practical networks. Based on this, we develop an analytical framework for the performance evaluation of small cell networks, adopting stochastic geometry theory. We derive the system coverage probability; average energy efficiency (AEE) and average uplink power consumption (AUPC) for different association strategies; maximum biased received power (MaBRP); and minimum association distance (MiAD). It is analytically shown that MaBRP is beneficial for coverage but will have some loss in energy saving. On the contrary, MiAD is not advocated from the point of coverage but is more energy efficient. The simulation results show that the use of range expansion in MaBRP helps to save energy but that this is not so in MiAD. Furthermore, we can achieve an optimal AEE by establishing an appropriate density of small cells.

• Membrane Journal
• /
• v.7 no.3
• /
• pp.142-149
• /
• 1997

The extended analysis on the diverging flow through asymmetric membrane pores has been performed in this study. Afore rigorous equations of velocity profile relevant to the divergent slit and cone shaped channels, which are widely used as a general pore model, have been obtained by employing a creeping flow approach of Newtonian fluids. As a degree of asymmetry (i.e., diverging angle) is increased, the predicted flow function shifts Toward the center region due to the incorporated wall effect, so that the overall velocity profile becomes decreased. It is true, as expected, that when the divergent channel is in the low diverging angle limit, the channel flow results in the Poiseuillean fashion by utilizing a lubrication approximation. The flow rate equation of each type of channel has been developed from the combined solution of velocity profile and pressure fields. The effect of diverging flow on the flow rate enhancement has been remarkably predicted, in which the flow rate increases with the increase of pore asymmetry. The advantage of our theoretical results lies in the analytical expression for the diverging flow behavior through pore channels as well as its ability to play a fundamental role on the related membrane filtrations such as microfiltration and ultrafiltration.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.19 no.1
• /
• pp.79-84
• /
• 1983

The test specimen, designated the double cantilever beam, was employed for a fracture mechanics study of stress corrosion cracking (SCC) of type 5083 Al-alloy in seawater. Stress intensities for this DCB specimen were calculated by using compliance, strain energy release rate and relation between stress intensity and strain energy release rate. Analytical expression for compliance as a function of crack length was obtained by applying beam theory. It was investigated that the polarization potentials affected the growth rate and surface of stress corrosion cracking. The results are as follows, The critical stress intensity was 134.81-148.38kg/mm super(3/2) and K sub(Ii) under polarization potentials was 75.92-145.78kg/mm super(3/2). The minimum stress corrosion crack growth rate was occurred at-987mV SCE. Insoluble compound on $\beta$ phase was looked into through SCC. The greater anodic potential is, the larger insoluble compound on $\beta$ phase becomes.

• Geophysics and Geophysical Exploration
• /
• v.25 no.1
• /
• pp.38-43
• /
• 2022

In case axial symmetrical bodies with varying cross sections such as volcanic conduits and unexploded ordnance (UXO), it is efficient to approximate them by adding the response of thin disks perpendicular to the axis of symmetry. To compute the vector magnetic and magnetic gradient tensor respones by such bodies, it is necessary to derive an analytical expression of the circular disk. Therefore, in this study, we drive closed-form expressions of the vector magnetic and magnetic gradient tensor due to a circular disk. First, the vector magnetic field is obtained from the existing gravity gradient tensor using Poisson's relation where the gravity gradient tensor due to the same disk with a constant density can be transformed into a magnetic field. Then, the magnetic gradient tensor is derived by differentiating the vector magnetic field with respect to the cylindrical coordinates converted from the Cartesian coordinate system. Finally, both the vector magnetic and magnetic gradient tensors are derived using Lipschitz-Hankel type integrals based on the axial symmetry of the circular disk.

• Steel and Composite Structures
• /
• v.44 no.2
• /
• pp.155-169
• /
• 2022

In the present paper an analytical model was developed to study the non-linear vibrations of Functionally Graded Carbon Nanotube (FG-CNT) reinforced doubly-curved shallow shells using the Multiple Scales Method (MSM). The nonlinear partial differential equations of motion are based on the FGM shallow shell hypothesis, the non-linear geometric Von-Karman relationships, and the Galerkin method to reduce the partial differential equations associated with simply supported boundary conditions. The novelty of the present model is the simultaneous prediction of the natural frequencies and their mode shapes versus different curvatures (cylindrical, spherical, conical, and plate) and the different types of FG-CNTs. In addition to combining the vibration analysis with optimization algorithms based on the genetic algorithm, a design optimization methode was developed to maximize the natural frequencies. By considering the expression of the non-dimensional frequency as an objective optimization function, a genetic algorithm program was developed by valuing the mechanical properties, the geometric properties and the FG-CNT configuration of shallow double curvature shells. The results obtained show that the curvature, the volume fraction and the types of NTC distribution have considerable effects on the variation of the Dimensionless Fundamental Linear Frequency (DFLF). The frequency response of the shallow shells of the FG-CNTRC showed two types of nonlinear hardening and softening which are strongly influenced by the change in the fundamental vibration mode. In GA optimization, the mechanical properties and geometric properties in the transverse direction, the volume fraction, and types of distribution of CNTs have a considerable effect on the fundamental frequencies of shallow double-curvature shells. Where the difference between optimized and not optimized DFLF can reach 13.26%.

• Structural Engineering and Mechanics
• /
• v.87 no.3
• /
• pp.255-272
• /
• 2023

Spatial cable systems can provide more transverse stiffness and torsional stiffness without sacrificing the vertical bearing capacity compared with conventional vertical cable systems, which is quite lucrative for long-span earth-anchored suspension bridges' development. Higher economy highlights the importance of refined form-finding analysis. Meanwhile, the internal connection between the lateral and vertical sags has not yet been specified. Given this, an analytic algorithm of form-finding for the earth-anchored suspension bridge with spatial cables is proposed in this paper. Through the geometric compatibility condition and mechanical equilibrium condition, the expressions for cable segment, the recurrence relationship between catenary parameters and control equations of spatial cable are established. Additionally, the nonlinear general reduced gradient method is introduced into fast and high-precision numerical analysis. Furthermore, the analytic expression of the lateral and vertical sags is deduced and discussed. This is very significant for the space design above the bridge deck and the optimization of the sag-to-span ratio in the preliminary design stage of the bridge. Finally, the proposed method is verified with the aid of two examples, one being an operational self-anchored suspension bridge (with spatial cables and a 260 m main span), and the other being an earth-anchored suspension bridge under design (with spatial cables and a 500 m main span). The necessity of an iterative calculation for hanger tensions on earth-anchored suspension bridges is confirmed. It is further concluded that the main cable and their connected hangers are in very close inclined planes.